IndraLab

Statements

USP9X affects MCL1
1 | 24
USP9X deubiquitinates MCL1. 10 / 24
1 | 23
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"USP9X deubiquitinates and stabilizes MCL1 in distinct human cancers including human follicular lymphomas, diffuse large B cell lymphomas, glioblastoma, colon and lung cancers 19."
29449692
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"Conversely, ubiquitination of Mcl1 can be reversed by the deubiquitinase USP9X and the more recently identified USP13 ."
30479840
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"USP9X interacts with and deubiquitinates MCL-1, thereby stabilising it."
34079080
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"It has been reported that Usp9x deubiquitinates Mcl-1 by removing the conjugated ubiquition."
24991768
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"The overexpression of USP9X stabilizes the MCL1 protein in human lymphomas, and the depletion of USP9X increases MCL1 ubiquitination, which leads to MM cell apoptosis [31]."
| PMC
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"In that study, USP9X, but not a catalytically inactive form of USP9X, was shown to decrease MCL1 ubiquitination in vitro."
23667531
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"Previous studies showed that USP9X deubiquitinates MCL1 and promotes cancer cell survival in human follicular lymphomas and diffuse large B cell lymphomas 19."
29449692
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"USP9X deubiquitinates and stabilizes MCL-1, a pro survival BCL2 family member XREF_BIBR, whose overexpression is associated with several neoplastic conditions XREF_BIBR - XREF_BIBR."
21283576
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"Disruption of Mcl-1:Noxa interaction followed by Noxa degradation, enhanced Mcl-1 de-ubiquitination by USP9x, and Mule destabilization are the major effects of these inhibitors."
36346691
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"USP9X deubiquitylation of MCL1 inhibits its proteasomal degradation, thus promoting its anti-apoptotic functions."
25672900
USP9X leads to the deubiquitination of MCL1-K40Q. 1 / 1
| 1
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"Given that USP9X inhibition by WP1130 restored poly-ubiquitination of acetylation-mimetic K40Q MCL1 (Figure 4C) and effectively reduced its protein abundance (Figures 4F and 4G), we next evaluated MCL1 levels in breast and prostate cancer cells treated with WP1130."
34758305
USP9X affects SMAD4
3 1 | 16
USP9X deubiquitinates SMAD4. 10 / 13
1 | 12
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"For example USP9X may upregulate ID2 gene expression by deubiquitinating and stabilizing the transcription factor SMAD4 (Dupont et al., 2009)."
21925315
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"The deubiquitination of Smad4 by FAM is direct."
19135894
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"FAM and USP9X is required for TGFbeta induced migration of MDA-MB-231 breast cancer cells; mechanistically, FAM and USP9X inhibits the monoubiquitination of SMAD4, a modification that blocks the association of SMAD4 with phospho-SMAD2 [XREF_BIBR] (XREF_FIG)."
27844253
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"Subsequently it was shown that USP9X deubiquitylates SMAD4 that is monoubiquitylated at K519 (XREF_FIG)."
25007997
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"Usp9x promotes TGFbeta pathway signalling by deubiquitylating Smad4, allowing it to complex with phosphorylated receptor Smads and then shuttle into the nucleus to execute transcriptional responses to TGFbeta family ligands [XREF_BIBR]."
25672900
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"Taken together, these results demonstrate that USP9x selectively binds to SMAD4 in competition with TIF1gamma and deubiquitinates SMAD4, promoting nuclear SMAD4 retention, SMAD3 and SMAD4 complex formation and target gene expression."
28115363
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"USP9X (also known as FAM) deubiquitylates SMAD4 and thereby sustains TGF-beta signaling (Dupont etal."
26085183
ubibrowser
"Multiple mono-ubiquitination of SMAD3 can be removed by USP15 and mono-ubiquitination of SMAD4 seems to be removed by FAM/USP9X."
25317929
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"This inhibitory event is readily reversed by FAM and USP9x, which deubiquitinates Smad4 and restores its responsiveness to TGF-beta [XREF_BIBR]."
20940046
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"USP9X reverses Smad4 ubiquitination to reactivate the pathway and reinstate TGFbeta signaling."
25606592
Modified USP9X leads to the deubiquitination of SMAD4. 3 / 3
| 3
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"Loss of USP9X therefore prevents deubiquitination of SMAD4, enhancing tumour progression."
24292195
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"Strikingly, overexpression of wild-type FAM, but not of the catalytically inactive FAM mutant, inhibited Smad4 monoubiquitination in vivo."
19135894
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"Overexpression of FAM also inhibits monoubiquitination of endogenous Smad4."
19135894
USP9X deubiquitinates SMAD4 on K519. 3 / 3
3 |
signor
"Smad4 is monoubiquitinated in lysine 519 in vivo, a modification that inhibits smad4 by impeding association with phospho-smad2. Fam reverts this negative modification, re-empowering smad4 function;control of smad4 is a good way to regulate bone formation. Fam and ectodermin/tif1gamma (ecto) were reported to respectively regulate the de-ubiquitination and ubiquitination of smad4."
19135894
signor
"Smad4 is monoubiquitinated in lysine 519 in vivo, a modification that inhibits smad4 by impeding association with phospho-smad2. Fam reverts this negative modification, re-empowering smad4 function;control of smad4 is a good way to regulate bone formation. Fam and ectodermin/tif1gamma (ecto) were reported to respectively regulate the de-ubiquitination and ubiquitination of smad4."
20016939
signor
"Smad4 is monoubiquitinated in lysine 519 in vivo, a modification that inhibits smad4 by impeding association with phospho-smad2. Fam reverts this negative modification, re-empowering smad4 function;control of smad4 is a good way to regulate bone formation. Fam and ectodermin/tif1gamma (ecto) were reported to respectively regulate the de-ubiquitination and ubiquitination of smad4."
22298955
USP9X deubiquitinates ubiquitinated SMAD4. 1 / 1
| 1
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"FAM and Usp9x deubiquitinates the monoubiquitinated SMAD4 (which is exported from the nucleus (XREF_FIG)) and thereby enables SMAD2 and SMAD4 complex formation."
25317929
USP9X affects SNCA
2 | 5
USP9X deubiquitinates SNCA. 7 / 7
2 | 5
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"We recently found that USP9X deubiquitinates alpha-synuclein, and that this process determines the partition of alpha-synuclein between the proteasomal and autophagy pathways."
22361583
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"Moreover, USP9X can increase MAPT phosphorylation via a second mechanism, by deubiquitinating the protein alpha-synuclein (SNCA) [XREF_BIBR], which functions as a connecting mediator between the glycogen synthase kinase 3beta (GSK3B) and MAPT and has been shown to stimulate MAPT phosphorylation via GSK3B in vitro [XREF_BIBR]."
27878758
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"In the brain tissues of PD patients, USP9X colocalises with alpha-synuclein inclusions, and in vitro studies show a functional interaction; whilst monoubiquitylated alpha-synuclein is degraded by the proteasome, USP9X deubiquitylation of alpha-synuclein directs its degradation by the less efficient autophagy pathway [XREF_BIBR]."
25672900
ubibrowser
"Deubiquitination of α-synuclein by USP9X impairs SIAH-dependent α-synuclein proteasomal degradation and promotes degradation by autophagy"
22065755
ubibrowser
"We recently found that USP9X deubiquitinates α-synuclein, and that this process determines the partition of α-synuclein between the proteasomal and autophagy pathways."
22361583
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"Usp9x also deubiquitylates mono-ubiquitylated alpha-synuclein raising the possibility it may play a role in the progression of neurodegenerative diseases such as Parkinson 's disease XREF_BIBR."
23861879
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"We now report that the deubiquitinase USP9X interacts in vivo with and deubiquitinates alpha-synuclein."
22065755
USP9X affects ERG
1 | 6
USP9X deubiquitinates ERG. 7 / 7
1 | 6
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"Previously, we found that ERG undergoes ubiquitination and then is deubiquitinated by USP9X in prostate cancer cells to prevent its proteasomal degradation."
27626314
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"Here, we show that ubiquitin specific peptidase 9, X linked (USP9X), a deubiquitinase enzyme, binds ERG in VCaP prostate cancer cells expressing TMPRSS2-ERG and deubiquitinates ERG in vitro."
24591637
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"USP9X binds to the ETS domain in ERG protein; therefore, USP9X deubiquitinates and stabilizes full-length ERG and TMPRSS2 - ERG fusion gene products."
30217566
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"Wang et al. demonstrated that ubiquitin specific peptidase 9, X linked (USP9X), a deubiquitinase enzyme, binds ERG in VCaP prostate cancer cells expressing TMPRSS2-ERG and deubiquitinates ERG in vitro XREF_BIBR."
27208692
reach
"A role for ERG ubiquitination in prostate cancer cells was also demonstrated by Wang et al. who showed that the enzyme USP9X, which is highly expressed in ERG positive prostate tumours, mediates ERG deubiquitination and thus its stabilization XREF_BIBR."
27208692
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"Indeed, previous reports have shown Usp9x deubiquitinates and stabilizes ERG, and our previously described DUB inhibitor (WP1130) demonstrated anti-tumour efficacy in ERG driven prostate cancer XREF_BIBR."
28198367
ubibrowser
"we show that ubiquitin-specific peptidase 9, X-linked (USP9X), a deubiquitinase enzyme, binds ERG in VCaP prostate cancer cells expressing TMPRSS2-ERG and deubiquitinates ERG in vitro."
24591637
USP9X affects IRS2
| 6
USP9X deubiquitinates IRS2. 6 / 6
| 6
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"Thus, it is possible that USP9X preferentially deubiquitinates IRS-2 much more than IRS-1 because of the difference of such ubiquitination patterns."
30338032
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"IRS-2 deubiquitination by USP9X maintains anchorage independent cell growth via Erk1/2 activation in prostate carcinoma cell line."
30338032
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"Knockdown of USP9X dramatically reduced IRS-2 protein level, increased IRS-2 ubiquitination, and promoted the proteasomal degradation of IRS-2 in PC3 cells, suggesting that USP9X also deubiquitinates IRS-2 to prevent its proteasomal degradation."
30338032
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"These data suggest that USP9X deubiquitinates IRS-2 and prevents its proteasomal degradation."
30338032
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"In addition, USP9X in LNCaP cells was not co-immunoprecipitated with IRS-2 (XREF_SUPPLEMENTARY), suggesting that USP9X does not deubiquitinate IRS-2 in LNCaP cells."
30338032
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"In addition, the interaction between USP9X and IRS-2 was not observed in LNCaP cells (XREF_SUPPLEMENTARY), suggesting that USP9X in LNCaP does not deubiquitinate and stabilize IRS-2 because of the absence of interaction between them."
30338032
USP9X affects ZBTB38
1 | 4
USP9X deubiquitinates ZBTB38. 5 / 5
1 | 4
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"Together these data show that USP9X deubiquitinates ZBTB38, both in basal conditions, and in conditions where ZBTB38 ubiquitination is augmented by RBBP6."
29490077
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"We thus conclude that USP9X activity triggers the deubiquitination of ZBTB38 in cells."
29490077
ubibrowser
"Molecularly, these functions depend on a deubiquitinase, USP9X, which interacts with ZBTB38, deubiquitinates it, and stabilizes it."
29490077
reach
"USP9X deubiquitinates ZBTB38."
29490077
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"By poly-ubiquitination assays, we observed that USP9X causes deubiquitination of ZBTB38, even in cells over-expressing RBBP6 and conversely that inactivation of USP9X amplifies the polyubiquitination induced by RBBP6 over-expression (XREF_SUPPLEMENTARY)."
29490077
USP9X affects YAP1
1 | 4
USP9X deubiquitinates YAP1. 5 / 5
1 | 4
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"Mechanistically, USP9X deubiquitinates and stabilizes YAP1."
29449692
ubibrowser
"Here we demonstrate that the deubiquitination enzyme USP9X deubiquitinates and stabilizes YAP1, thereby promoting cancer cell survival."
29449692
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"Here we demonstrate that the deubiquitination enzyme USP9X deubiquitinates and stabilizes YAP1, thereby promoting cancer cell survival."
29449692
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"Since YAP1 plays a key role in human cancer development, it is possible that USP9X promotes deubiquitination and stabilization of YAP1 in human cancers."
29449692
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"Mechanistically, it was found that hsa_circ_0024093 could regulate the expression of USP9X, which further induced YAP1 deubiquitination to stabilize YAP1 protein."
34631281
USP9X affects CTNNB1
1 | 1 3
USP9X deubiquitinates CTNNB1. 5 / 5
1 | 1 3
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"As shown in Figure XREF_FIG, knockdown of USP9X increased the ubiquitination of beta-catenin and subsequent degradation."
27783990
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"Deubiquitinase USP9X deubiquitinates beta-catenin and promotes high grade glioma cell growth."
27783990
trips
"Deubiquitinase USP9X deubiquitinates β-catenin and promotes high grade glioma cell growth."
27783990
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"In the Wnt pathway, FAM deubiquitinates betacatenin, preventing its degradation in mammalian cells, but the effect of FAM activity on Wnt signaling was not noted."
18797952
ubibrowser
"Additionally, DUBs USP14, USP15, USP47, and Fam/USP9X have been reported to prevent β-catenin turnover by inhibiting its Ub-proteasomal degradation"
29080116
USP9X affects AMOT
| 5
USP9X deubiquitinates AMOT on K496. 2 / 2
| 2
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"USP9x acts to deubiquitylate Angiomotin at lysine 496, resulting in stabilization of Angiomotin and lower YAP and TAZ activity."
27462448
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"USP9X leads to deubiquitinate AMOT at Lys 496, resulting in stabilization of AMOT and reduced YAP and TAZ activity."
29366444
USP9X deubiquitinates AMOT. 2 / 2
| 2
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"USP9X in the DUBs (ubiquitin-specific protease family) could bind to and deubiquitinate AMOT."
34539895
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"Thus, it appears that the direct effect of limiting deubiquitylation of AMOT by USP9x offsets the reduced potential for ubiquitylation due to lower E3 ligase levels."
27462448
Modified USP9X leads to the deubiquitination of AMOT. 1 / 1
| 1
reach
"We demonstrate that loss of USP9x leads to increased ubiquitylation of AMOT, resulting in reduced AMOT levels."
27462448
USP9X affects XIAP
| 4
USP9X deubiquitinates XIAP. 3 / 3
| 3
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"Engel et al. [XREF_BIBR] demonstrate that USP9X is the mitotic deubiquitinase of the X linked inhibitor of apoptosis protein (XIAP) and that deubiquitylation and stabilization of XIAP by USP9X lead to increased resistance toward mitotic spindle poisons."
27766120
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"These studies revealed a mitosis specific function for the ubiquitin specific protease 9X (USP9X), which we show to deubiquitylate and stabilize XIAP in order to promote mitotic survival."
27317434
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"We reasoned that USP9X deubiquitylates XIAP to regulate mitotic survival."
27317434
Modified USP9X leads to the deubiquitination of XIAP. 1 / 1
| 1
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"Indeed, ubiquitylation of XIAP was substantially increased upon silencing or chemical inhibition of USP9X (Figs XREF_FIG E and XREF_FIG A) in mitotic cells, while forced expression of USP9X attenuated XIAP ubiquitylation (Fig XREF_FIG A)."
27317434
USP9X affects TDRD3
| 4
USP9X deubiquitinates TDRD3. 4 / 4
| 4
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"To test whether USP9X de-ubiquitinates TDRD3 in cells, we transfected HeLa cells with either control or USP9X specific siRNA, and measured TDRD3 ubiquitination."
28101374
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"We observed that when the cellular levels of USP9X were reduced by siRNA, the TDRD3 ubiquitination levels markedly increased (XREF_FIG, upper panel -- compare lane 2 to lane 5) and inhibition of the proteasome greatly augmented this difference (XREF_FIG, upper panel -- compare lane 3 to lane 6), suggesting that USP9X is necessary to suppress TDRD3 ubiquitination."
28101374
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"USP9X prevents TDRD3 ubiquitination."
28101374
reach
"USP9X prevents polyubiquitination of TDRD3 in cells."
28101374
USP9X affects SMURF1
1 | 3
USP9X deubiquitinates SMURF1. 4 / 4
1 | 3
ubibrowser
"Review"
27775833
reach
"Moreover, USP9X deubiquitinates and stabilizes SMURF1, a member of the NEDD4 family of ubiquitin ligases; silencing USP9X expression in MDA-MB-231 breast cancer cells destabilized SMURF1 and inhibited SMURF1 dependent cell migration [XREF_BIBR]."
27844253
reach
"USP9X (FAM) deubiquitylates the autoubiquitylated E3 ligases Itch and SMURF1 and, thereby, increases their stability XREF_BIBR - XREF_BIBR."
24714733
reach
"In another study, in breast cancer cells ubiquitination of Smurf1 could be reversed by the deubiquitinating enzyme USP9X through Smurf1 WW domain binding, which improved Smurf1 's stability."
33718111
USP9X affects IQCB1
1 | 3
USP9X deubiquitinates IQCB1. 3 / 3
1 | 2
reach
"Because USP9X deubiquitinates NPHP5 (XREF_FIG), we hypothesize that NPHP5 is prone to ubiquitination when its association with USP9X is compromised in G2/M."
28498859
reach
"Only wild type USP9X could robustly deubiquitinate NPHP5 (XREF_FIG)."
28498859
ubibrowser
"NPHP5 directly binds to a deubiquitinating enzyme USP9X/FAM and two E3 ubiquitin ligases BBS11/TRIM32 and MARCH7/axotrophin. NPHP5 undergoes K63 ubiquitination in a cell cycle dependent manner and K48/K63 ubiquitination upon USP9X depletion or inhibition."
28498859
USP9X deubiquitinates IQCB1 on K48. 1 / 1
| 1
reach
"On the basis of our findings that NPHP5 is deubiquitinated by USP9X, K48 ubiquitinated by MARCH7 and K63 ubiquitinated by BBS11, we asked whether simultaneous ablation of MARCH7 and/or BBS11 might override the effects of USP9X loss on NPHP5."
28498859
USP9X affects STIL
| 3
USP9X deubiquitinates STIL. 3 / 3
| 3
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"As USP9X interacts with and deubiquitylates the MD3 domain (aa 715-988) of STIL and removal of the MD3 domain destabilized STIL, USP9X controls STIL levels via the MD3 antidegron of STIL."
31197030
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"To test whether USP9X deubiquitylates the STIL MD3 domain, we incubated immunoprecipitated full-length STIL or the MD3 domain of STIL with recombinant GST, GST-USP7, or GST fused to the catalytic domain of USP9X (USP9X CD)."
31197030
reach
"USP9X directly deubiquitylates STIL."
31197030
USP9X affects SOX2
1 | 2
USP9X deubiquitinates SOX2. 3 / 3
1 | 2
reach
"We identified that Usp9x deubiquitinates SOX2 and can increaseSOX2 levels, but additional studies are needed to confirm the specific ubiquitin sites among the 16 lysine residues in SOX2 that could be putative ubiquitin acceptors."
33613844
ubibrowser
"Review"
27919796
reach
"Usp9x knockdown in melanoma increased SOX2 ubiquitination, leading to its depletion, and enhanced apoptotic effects of BRAF inhibitor and MEK inhibitors."
33613844
USP9X affects Parkinson Disease
| 3
USP9X leads to the deubiquitination of Parkinson Disease on L1. 3 / 3
| 3
reach
"Taken together, these findings suggest that activated tumor signaling pathways vary in different tumor microenvironments.Interestingly, USP9X reduced the ubiquitination of PD‐L1 and stabilized its protein expression."
29992764
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"Ubiquitin‐specific peptidase 9, X‐linked (USP9X) inhibited the ubiquitination and degradation of PD‐L1, thereby mediating the immune escape of OSCC."
29992764
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"The results further validated our hypothesis that the highly expressed USP9X increased and decreased the deubiquitination and ubiquitination of PD‐L1, respectively, leading to its accumulation in OSCC cell lines.3.5 USP9X is critical in OSCC tumor growth."
29992764
USP9X affects PRC2_complex
| 3
USP9X deubiquitinates PRC2_complex. 3 / 3
| 3
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"We report here that Usp9x deubiquitinates and stabilizes PRC2, acting as a gatekeeper to the switch in H3K27me3 deposition patterns during mouse development."
33767158
reach
"Usp9x deubiquitinates and stabilizes PRC2."
33767158
reach
"This finding led us to hypothesize that Usp9x deubiquitinates and stabilizes PRC2 components to drive H3K27me3 deposition."
33767158
USP9X affects MIB1
1 | 2
USP9X deubiquitinates MIB1. 3 / 3
1 | 2
reach
"Further, in human breast cancer cell lines, USP9X deubiquitinates and stabilizes MIB1, an activator of ligand dependent canonical NOTCH1 signaling that is important for cardiac development."
33547080
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"Therefore, we postulated that nitrosylated USP9X deubiquitinates and stabilizes MIB1 in valvular interstitial cells, which potentiates the ability of a ligand producing cell to activate NOTCH on a neighboring cell."
33547080
ubibrowser
"USP9x in turn stimulates JAG1 activity through two mechanisms: (1) through TRB3 deubiquitination and stabilization, and (2) through deubiquitination and activation of Mind Bomb 1, an E3 ligase required for JAG1 ubiquitination-mediated endocytosis and Notch activation."
27593927
USP9X affects ITCH
1 | 2
USP9X deubiquitinates ITCH. 3 / 3
1 | 2
reach
"USP9X (FAM) deubiquitylates the autoubiquitylated E3 ligases Itch and SMURF1 and, thereby, increases their stability XREF_BIBR - XREF_BIBR."
24714733
reach
"USP8 and USP9X deubiquitinate ITCH to induce ubiquitination and degradation of the anti-apoptotic protein c-FLIP, leading to apoptosis in glioblastoma [XREF_BIBR], or to anoikis in pancreatic ductal adenocarcinoma [XREF_BIBR]."
27203743
ubibrowser
"Review"
27775833
USP9X affects IGF1R
| 3
USP9X deubiquitinates IGF1R. 3 / 3
| 3
reach
"These results raise the possibility that USP9X deubiquitinates IGF-IR to prevent its degradation."
30338032
reach
"We found that USP9X interacted with IGF-IR, and that knockdown of USP9X decreases IGF-IR protein level and increased ubiquitination of IGF-IR."
30338032
reach
"Furthermore, knockdown of USP9X significantly increased IGF-IR ubiquitination in HEK293T cells."
30338032
USP9X affects GJA1
| 1 2
USP9X deubiquitinates GJA1. 3 / 3
| 1 2
trips
"USP9X deubiquitinates connexin43 to prevent high glucose-induced epithelial-to-mesenchymal transition in NRK-52E cells."
33857489
reach
"The following findings were observed : (1) Expression of USP9X was down-regulated in the kidney tissue of db/db diabetic mice; (2) overexpression of USP9X suppressed high glucose (HG)-induced expressions of EMT markers and extra cellular matrix (ECM) in NRK-52E cells; (3) depletion of USP9X further aggravated EMT process and ECM production in NRK-52E cells; (4) USP9X deubiquitinated Cx43 and suppressed its degradation to regulate EMT process; (5) USP9X deubiquitinated Cx43 by directly binding to the C-terminal Tyr 286 of Cx43."
33857489
reach
"The results showed that ubiquitin-specific protease 9 X-chromosome (USP9X), a deubiquitylating enzyme, was a candidate of Cx43 binding proteins, and USP9X inhibited Cx43 ubiquitination."
35391932
USP9X affects EPS15
1 1 | 1
USP9X deubiquitinates EPS15. 3 / 3
1 1 | 1
signor
"We identify the endocytic protein Eps15 as one of the critical substrates of USP9X"
26748853
ubibrowser
"USP9X Controls EGFR Fate by Deubiquitinating the Endocytic Adaptor Eps15"
26748853
reach
"Interestingly, recent findings further demonstrate that EPS15 de-ubiquitination by USP9X affects EGFR internalization and its trafficking to lysosomes."
27008177
USP9X affects CEP131
1 | 2
USP9X deubiquitinates CEP131. 3 / 3
1 | 2
reach
"USP9X gain-of-function leads to CEP131 deubiquitylation, stabilisation and centrosome amplification [XREF_BIBR]."
28900014
reach
"USP9X deubiquitinates CEP131."
28361952
ubibrowser
"USP9X can also regulate and stabilize CEP131, a centriolar satellite protein, ultimately promoting breast carcinogenesis, indicating that USP9X is a significant regulator of centrosome biogenesis and revealing a critical role for the USP9X/CEP131 axis in breast carcinogenesis"
30319415
USP9X affects CD274
| 3
USP9X deubiquitinates CD274. 2 / 2
| 2
reach
"Our data indicate that USP9X deubiquitinates and stabilizes PD-L1."
29992764
reach
"Indeed, USP9X induces PD-L1 deubiquitination and regulates its stabilization by ubiquitin specific protease activity."
32850399
Modified USP9X leads to the deubiquitination of CD274. 1 / 1
| 1
reach
"In Oral Squamous Cell Carcinoma (OSCC) cells, the high expression of USP9X increases the deubiquitination of PD-L1 and reduces its degradation, resulting in protein accumulation in these cells."
32850399
USP9X affects AMOTL2
1 | 2
USP9X deubiquitinates AMOTL2. 3 / 3
1 | 2
reach
"These results indicate that deubiquitination of AMOTL2 by USP9X depresses LATS1/2 activity and subsequently activates YAP."
29366444
reach
"Importantly, USP9X depletion increased AMOTL2 mono-ubiquitination, an effect that was abolished by co-depletion of WWP1 using two independent small hairpin RNAs (shRNAs), confirming that the two enzymes indeed exhibit contrasting functions on the same AMOTL2 lysine residues (Fig S2B)."
34404733
ubibrowser
"we report for the first time that USP9X is a deubiquitinase of Angiomotin-like 2 (AMOTL2) and that AMOTL2 mono-ubiquitination is required for YAP inhibition."
26598551
USP9X affects epnA
| 2
USP9X deubiquitinates epnA. 2 / 2
| 2
reach
"It was recently shown that silencing Fam by siRNA abrogated ionomycin induced decrease in Epsin ubiquitylation, indicating that Fam plays a role in regulating levels of Epsin ubiquitylation [15]."
15694383
reach
"However, in vitro data on direct deubiquitylation of Epsin by Fam are lacking, and the ~ 50% identity between Faf and Fam suggests different specificities for substrates [49]; indeed, the only reporte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15694383
USP9X affects TP53
1 | 1
USP9X leads to the deubiquitination of TP53. 2 / 2
1 | 1
ubibrowser
"Review"
28801249
reach
"USP9X inhibited p53 ubiquitination mediated degradation."
28446729
USP9X affects PEF1
| 2
USP9X deubiquitinates PEF1. 1 / 1
| 1
reach
"As USP9X, but not USP9X , deubiquitylated PEF1 in vitro (Figure 6G), we conclude that PEF1 ubiquitylation is removed by USP9X."
35120648
Ubiquitinated USP9X leads to the deubiquitination of PEF1. 1 / 1
| 1
reach
"In vitro ubiquitylation and USP9X-mediated deubiquitylation of PEF1."
35120648
USP9X affects NUAK1
1 | 1
USP9X leads to the deubiquitination of NUAK1. 2 / 2
1 | 1
reach
"An investigation of the polyubiquitination of AMPK-RKs showed that the deubiquitinating enzyme USP9X (ubiquitin specific protease-9) modulates the deubiquitination of NUAK1 (AMPK related kinase 5) and MARK4 (microtubule-affinity-regulating kinase 4) in cells XREF_BIBR."
23029325
ubibrowser
"Review"
27775833
USP9X affects NRP1
| 2
USP9X leads to the deubiquitination of NRP1. 2 / 2
| 2
reach
"Moreover, USP9X was found to be a critical deubiquitinating enzyme for the stability and high activity of NRP1 and NRP1 deubiquitination mediated by USP9X enhanced HSC activation and liver fibrosis."
36653359
reach
"NRP1 deubiquitination mediated by USP9X enhances HSC activation, implying that targeting NRP1 or USP9X potentiates novel options in the treatment of liver fibrosis."
36653359
USP9X affects MTOR
1 | 1
USP9X deubiquitinates MTOR. 2 / 2
1 | 1
reach
"USP9X deubiquitination of the mTOR (mechanistic target of rapamycin) signaling component RAPTOR protects it from proteasomal degradation, thus promoting mTOR signaling and proliferation of NSCs (34)."
35662507
ubibrowser
"Review"
27775833
USP9X affects MARK4
1 | 1
USP9X leads to the deubiquitination of MARK4. 2 / 2
1 | 1
reach
"An investigation of the polyubiquitination of AMPK-RKs showed that the deubiquitinating enzyme USP9X (ubiquitin specific protease-9) modulates the deubiquitination of NUAK1 (AMPK related kinase 5) and MARK4 (microtubule-affinity-regulating kinase 4) in cells XREF_BIBR."
23029325
ubibrowser
"Review"
27775833
USP9X affects MARCHF7
1 | 1
USP9X deubiquitinates MARCHF7. 2 / 2
1 | 1
ubibrowser
"Review"
27775833
reach
"Exogenous expression and short interfering RNA depletion experiments demonstrate that MARCH7 can be stabilized by both USP9X and USP7, which deubiquitylate MARCH7 in the cytosol and nucleus, respectively."
18410486
USP9X affects LATS2
1 | 1
USP9X deubiquitinates LATS2. 2 / 2
1 | 1
reach
"We further demonstrated that USP9X deubiquitinates LATS2 and thus prevents LATS2 degradation by the proteasome."
29183995
ubibrowser
"Deubiquitylase USP9X suppresses tumorigenesis by stabilizing large tumor suppressor kinase 2 (LATS2) in the Hippo pathway"
29183995
USP9X affects FBXW7
| 2
USP9X leads to the deubiquitination of FBXW7. 2 / 2
| 2
reach
"USP9X antagonized FBW7 ubiquitylation and protected mice from CRC."
29346117
reach
"USP9X antagonized FBW7 ubiquitylation, and Usp9x deletion caused Fbw7 destabilization."
29346117
USP9X affects F2R
| 2
USP9X deubiquitinates F2R. 2 / 2
| 2
reach
"To test whether FAF directly de-ubiquitinates PAR-1, we used affinity purified FAF and HA-Ub-labelled PAR-1 in an in vitro reaction."
23271647
reach
"FAF clearly reduced poly-ubiquitinated PAR-1 level in vitro (XREF_FIG), supporting that FAF directly de-ubiquitinates PAR-1."
23271647
USP9X affects ETS1
1 | 1
USP9X leads to the deubiquitination of ETS1. 2 / 2
1 | 1
reach
"A recent report demonstrated that USP9X prevents ETS1 ubiquitination and thereby stabilizes the protein [XREF_BIBR]."
28474232
ubibrowser
"Ets-1 deubiquitination blocks its proteasomal destruction and enhances tumorigenicity, which could be reversed by Usp9x knockdown or inhibition."
28198367
USP9X affects CDC20
| 2
USP9X deubiquitinates CDC20. 2 / 2
| 2
reach
"In summary, the data presented here show that USP9X antagonizes Cdc20 auto-ubiquitination and degradation to limit the turnover of MCC complexes by APC/C."
29669289
reach
"We hypothesize that USP9X deubiquitinates Cdc20 directly; however, we can not rule out the possibility that USP9X loss enhances Cdc20 ubiquitination and degradation indirectly."
29669289
USP9X affects BIRC5
| 2
USP9X deubiquitinates BIRC5. 1 / 1
| 1
reach
"Previously, Chen et al. reported that USP9X is involved in deubiquitination and degradation of survivin by inhibition of long noncoding RNA LNC473 [41]."
32825566
USP9X deubiquitinates BIRC5 on lysine. 1 / 1
| 1
reach
"Lysine 63 de-ubiquitination of survivin by hFAM is required for the dissociation of survivin from centromeres."
32410646
USP9X affects BCL9
| 2
USP9X deubiquitinates BCL9. 2 / 2
| 2
reach
"The USP9X mediated BCL9 deubiquitination promotes formation of the beta, catenin, BCL9, and PYGO complex, increasing the transcriptional activity of the Wnt and beta-catenin target genes."
32486158
reach
"Additionally, the deubiquitination of BCL9 by USP9X increases proliferation and invasion of breast cancer cells."
32486158
USP9X affects BCL10
1 | 1
USP9X leads to the deubiquitination of BCL10. 2 / 2
1 | 1
ubibrowser
"Mechanistically, USP9X interacts with Bcl10 of the Carma1-Bcl10-Malt1 (CBM) complex and removes the TCR-induced ubiquitin chain from Bcl10, which facilitates the association of Carma1 with Bcl0-Malt1."
23690623
reach
"Finally, the ubiquitin specific protease 9X (USP9X) interacts with BCL10 in activated T cells and silencing of USP9X augments BCL10 ubiquitination and impairs NF-kappaB signaling in T cells."
30022982
USP9X affects ARNTL
1 | 1
USP9X leads to the deubiquitination of ARNTL. 2 / 2
1 | 1
reach
"Through biochemical experiments, we discover that USP9X reduces BMAL1 ubiquitination, enhances its stability, and increases its protein level, leading to the elevated transcriptional activity."
29626158
ubibrowser
"Here, we use affinity purification and mass spectrometry analysis to identify probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X) as an interacting protein of the core clock protein aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL or BMAL1)."
29626158
USP9X affects pygo
| 1
USP9X leads to the deubiquitination of pygo. 1 / 1
| 1
reach
"The USP9X mediated BCL9 deubiquitination promotes formation of the beta, catenin, BCL9, and PYGO complex, increasing the transcriptional activity of the Wnt and beta-catenin target genes."
32486158
USP9X affects core PRC2 members
| 1
USP9X deubiquitinates core PRC2 members. 1 / 1
| 1
reach
"In summary, we report here that Usp9x deubiquitinates core PRC2 members to promote high levels of H3K27me3, repress developmental regulatory genes and maintain a preimplantation like phenotype in ES cells."
33767158
USP9X affects alpha-syn
| 1
USP9X deubiquitinates alpha-syn. 1 / 1
| 1
reach
"Remarkably, de-ubiquitination of alpha-syn by USP9X determines the partition of alpha-syn between the UPS and autophagy pathways."
32344772
USP9X affects ZAP70
1 |
USP9X deubiquitinates ZAP70. 1 / 1
1 |
ubibrowser
"Usp9X becomes competent to deubiquitinate ZAP70 through TCR-dependent phosphorylation and enhancement of its catalytic activity and association with the LAT signalosome."
26936881
USP9X affects TTK
1 |
USP9X deubiquitinates TTK. 1 / 1
1 |
ubibrowser
"Conclusions: In summary, our data demonstrated that the USP9X-TTK axis may play a critical role in NSCLC, and could be considered as a potential therapeutic target."
29721084
USP9X affects TRIB3
1 |
USP9X deubiquitinates TRIB3. 1 / 1
1 |
ubibrowser
"USP9x in turn stimulates JAG1 activity through two mechanisms: (1) through TRB3 deubiquitination and stabilization, and (2) through deubiquitination and activation of Mind Bomb 1, an E3 ligase required for JAG1 ubiquitination-mediated endocytosis and Notch activation."
27593927
USP9X affects TMPRSS2
| 1
USP9X deubiquitinates TMPRSS2. 1 / 1
| 1
reach
"USP9X binds to the ETS domain in ERG protein; therefore, USP9X deubiquitinates and stabilizes full-length ERG and TMPRSS2 - ERG fusion gene products."
30217566
USP9X affects TGFBR2
| 1
USP9X deubiquitinates TGFBR2. 1 / 1
| 1
reach
"Mechanically, USP9X positively influences the expression of TGFBR2 at different levels through two independent ways: (i) directly targets and deubiquitinates TGFBR2, which maintains the protein stability of TGFBR2 through avoiding degradation mediated by ubiquitin-proteasome system; (ii) indirectly maintains TGFBR2 messenger RNA (mRNA) expression via SMAD4/miR-143 axis."
35578792
USP9X affects TAS2R13
1 |
USP9X deubiquitinates TAS2R13. 1 / 1
1 |
ubibrowser
"USP9x in turn stimulates JAG1 activity through two mechanisms: (1) through TRB3 deubiquitination and stabilization, and (2) through deubiquitination and activation of Mind Bomb 1, an E3 ligase required for JAG1 ubiquitination-mediated endocytosis and Notch activation."
27593927
USP9X affects STUB1
| 1
USP9X leads to the deubiquitination of STUB1. 1 / 1
| 1
reach
"Like PHF-tau, mono- or polyubiquitination involves CHIP, whereas USP9X leads to the deubiquitination of CHIP-monoubiquitinated α-synuclein [48]."
35406655
USP9X affects STMN1
1 |
USP9X deubiquitinates STMN1. 1 / 1
1 |
ubibrowser
"In this report, we find that Usp9x, a deubiquitinating enzyme, stably associates with the SMN complex via directly interacting with SMN.| Usp9x deubiquitinates SMN that is mostly mono- and di-ubiquitinated."
23112048
USP9X affects STAM
| 1
USP9X deubiquitinates STAM. 1 / 1
| 1
reach
"In addition, we identified STAM and NFX1, which are known to be deubiquitylated by USP8 and USP9 respectively."
33417828
USP9X affects SNRPN
| 1
USP9X deubiquitinates SNRPN. 1 / 1
| 1
reach
"Usp9x deubiquitinates SMN that is mostly mono- and di ubiquitinated."
23112048
USP9X affects SNAI1
| 1
USP9X deubiquitinates SNAI1. 1 / 1
| 1
reach
"Here, we demonstrate that the deubiquitinating enzyme USP9X deubiquitinates and stabilizes Snail1, thereby promoting metastasis and chemoresistance."
35506169
USP9X affects SMN1
1 |
USP9X deubiquitinates SMN1. 1 / 1
1 |
signor
"Ubiquitin-specific Protease 9x Deubiquitinates and Stabilizes the Spinal Muscular Atrophy Protein-Survival Motor Neuron"
23112048
USP9X affects SMAD1
1 |
USP9X deubiquitinates SMAD1. 1 / 1
1 |
USP9X affects PSD
1 |
USP9X deubiquitinates PSD. 1 / 1
1 |
ubibrowser
"USP9x-mediated deubiquitination of EFA6 regulates de novo tight junction assembly"
20339350
USP9X affects PRICKLE2
| 1
USP9X deubiquitinates PRICKLE2. 1 / 1
| 1
reach
"[XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR] We postulated the physical interaction between the PRICKLEs and USP9X might indicate that PRICKLE was a USP9X substrate, and that USP9X deubiquitinates and stabilizes both PRICKLE1 and PRICKLE2."
25763846
USP9X affects PRICKLE1
| 1
USP9X deubiquitinates PRICKLE1. 1 / 1
| 1
reach
"[XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR] We postulated the physical interaction between the PRICKLEs and USP9X might indicate that PRICKLE was a USP9X substrate, and that USP9X deubiquitinates and stabilizes both PRICKLE1 and PRICKLE2."
25763846
USP9X affects PRICKLE
| 1
USP9X deubiquitinates PRICKLE. 1 / 1
| 1
reach
"PRICKLE and USP9X interact through their carboxy-termini; and USP9X de-ubiquitinates PRICKLE, protecting it from proteasomal degradation."
25763846
USP9X affects PEX5
1 |
USP9X deubiquitinates PEX5. 1 / 1
1 |
ubibrowser
"In addition, Pex5p can be deubiquitinated by the deubiquitinase USP9X"
26367801
USP9X affects PEG10
| 1
USP9X deubiquitinates PEG10. 1 / 1
| 1
reach
"PEG10 deubiquitination by USP9X appeared to stabilize PEG10-RF1 specifically because USP9X-deficient ESCs contained less PEG10-RF1 than control ESCs, whereas the amount of PEG10-RF1/2 was largely unaltered (Fig 1B and 1G)."
30951545
USP9X affects PCM1
1 |
USP9X deubiquitinates PCM1. 1 / 1
1 |
ubibrowser
"?Collectively, our experiments identified USP9X as an integral component of the centrosome where it functions to stabilize PCM1 and CEP55 and promote centrosome biogenesis."
28620049
USP9X affects NFX1
| 1
USP9X deubiquitinates NFX1. 1 / 1
| 1
reach
"In addition, we identified STAM and NFX1, which are known to be deubiquitylated by USP8 and USP9 respectively."
33417828
USP9X affects NFE2L2
| 1
USP9X leads to the deubiquitination of NFE2L2. 1 / 1
| 1
reach
"Taken together, USP9X reduced Nrf2 ubiquitination level and promoted Nrf2-ARE pathway activation to prevent the accumulation of extracellular matrix, eventually alleviated the pathological process of diabetic renal fibrosis."
32442538
USP9X affects MCL-1 [
| 1
USP9X leads to the deubiquitination of MCL-1 [. 1 / 1
| 1
reach
"Ubiquitin specific peptidase 9 X linked, USP9X, reverses ubiquitination of MCL-1 [XREF_BIBR]."
23958396
USP9X affects MAP3K5
1 |
USP9X deubiquitinates MAP3K5. 1 / 1
1 |
USP9X affects Liquid facets
| 1
USP9X leads to the deubiquitination of Liquid facets. 1 / 1
| 1
reach
"The study in fly Drosophila indicates that Fat facets (Usp9 ortholog) promotes endocytosis of Delta by deubiquitylating Liquid facets (Epsin ortholog) XREF_BIBR - XREF_BIBR."
24714733
USP9X affects LATS1
1 |
USP9X deubiquitinates LATS1. 1 / 1
1 |
ubibrowser
"In addition, USP9X can also function as a tumor suppressor. USP9X strongly interacts with LATS, a core kinase in the Hippo pathway. Increased USP9X expression significantly up-regulates and stabilizes LATS and leads to a decrease in the transport of YAP/TAZ into the nucleus as well as inhibiting of their target genes."
30319415
USP9X affects KCNH2
1 |
USP9X deubiquitinates KCNH2. 1 / 1
1 |
USP9X affects IRS1
| 1
USP9X deubiquitinates IRS1. 1 / 1
| 1
reach
"On the other hand, the reduction of IRS-1 protein level was relatively small, suggesting that deubiquitination of IRS-1 by USP9X is not so active as for IRS-2."
30338032
USP9X affects GPSM1
1 |
USP9X deubiquitinates GPSM1. 1 / 1
1 |
USP9X affects FOXO3
1 |
USP9X deubiquitinates FOXO3. 1 / 1
1 |
ubibrowser
"Hydroxylation of these sites prevents the binding of USP9x deubiquitinase, thereby promoting the proteasomal degradation of FOXO3a."
24990963
USP9X affects FN1
| 1
USP9X leads to the deubiquitination of FN1. 1 / 1
| 1
reach
"Importantly, USP9x depletion augmented and maintained the FN induced integrin alpha5beta1 complex ubiquitination and led to a slower migration rate, suggesting that USP9x contributes to deubiquitinati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26456826
USP9X affects F3
| 1
USP9X deubiquitinates F3. 1 / 1
| 1
reach
"Usp9x deubiquitinates and stabilizes the TF, ERG, in prostate, and we previously published that DUB inhibitor (WP1130) has anti-tumor activity in ERG fusion driven prostate cancer [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
33613844
USP9X affects EWS-FLI1
| 1
USP9X deubiquitinates EWS-FLI1. 1 / 1
| 1
reach
"We show that USP9X binds the ETS domain of EWS-FLI1 in Ewing sarcoma cells and deubiquitinates EWS-FLI1 and that USP9X and EWS-FLI1 protein expression is correlated in clinical Ewing sarcoma specimens."
36330954
USP9X affects ERBB2
1 |
USP9X deubiquitinates ERBB2. 1 / 1
1 |
ubibrowser
"?In contrast, ErbB2 immunoprecipitates were found to contain significant USP9x relative to control immunoprecipitates prepared using anti-ERalpha IgG"
20406983
USP9X affects EPN
| 1
USP9X deubiquitinates EPN. 1 / 1
| 1
reach
"Agonist stimulation of Notch leads to the recruitment of USP9X, a deubiquitinating enzyme, which deubiquitinates epsin and enables it to promote Notch internalization."
21438117
USP9X affects EIF4B
1 |
USP9X deubiquitinates EIF4B. 1 / 1
1 |
ubibrowser
"USP9X controls translation efficiency via deubiquitination of eukaryotic translation initiation factor 4A1"
29228324
USP9X affects EIF4A2
1 |
USP9X deubiquitinates EIF4A2. 1 / 1
1 |
ubibrowser
"Our results provide evidence that USP9X is a novel regulator of the translation initiation process via deubiquitination of eIF4A1"
29228324
USP9X affects EIF4A1
| 1
USP9X deubiquitinates EIF4A1. 1 / 1
| 1
reach
"These data suggested that the deubiquitination of eIF4A1 by USP9X is required for eIF4A1 protein stability."
29228324
USP9X affects EGFR
| 1
USP9X leads to the deubiquitination of EGFR. 1 / 1
| 1
reach
"However, silencing of USP9X did not increase EGFR ubiquitination, arguing that the receptor itself is not the direct target."
26748853
USP9X affects DVL2
1 |
USP9X deubiquitinates DVL2. 1 / 1
1 |
ubibrowser
"Our findings indicate that USP9X-mediated deubiquitylation of DVL2 is required for canonical WNT activation, while increased DVL2 ubiquitylation is associated with localization to actin-rich projections and activation of the planar cell polarity (PCP) pathway."
31340145
USP9X affects DDI1
1 |
USP9X deubiquitinates DDI1. 1 / 1
1 |
ubibrowser
"Identification of USP9X as the DUB Responsible for Deubiquitination of Human DDI1"
31130875
USP9X affects CEP55
1 |
USP9X deubiquitinates CEP55. 1 / 1
1 |
ubibrowser
"?Collectively, our experiments identified USP9X as an integral component of the centrosome where it functions to stabilize PCM1 and CEP55 and promote centrosome biogenesis."
28620049
USP9X affects CDH1
1 |
USP9X deubiquitinates CDH1. 1 / 1
1 |
USP9X affects CDC123
| 1
USP9X deubiquitinates CDC123. 1 / 1
| 1
reach
"USP9X deubiquitinates and stabilizes CDC123 to promote breast carcinogenesis through regulating cell cycle."
37314216
USP9X affects BRCA1
| 1
USP9X leads to the deubiquitination of BRCA1. 1 / 1
| 1
reach
"Immunoblotting analysis showed that USP9X knockdown significantly increased the ubiquitination of BRCA1 protein (Figure 2D).3.3 USP9X interacts with BRCA1."
31512408
USP9X affects BECN1
1 |
USP9X deubiquitinates BECN1. 1 / 1
1 |
ubibrowser
"Interestingly, Beclin1 and the Bcl-2 family member, MCL-1, compete for their interaction with USP9X, which contributes to their stabilization by protecting them from proteasomal degradation in HEK293T cells"
30126257
USP9X affects APC_C
| 1
USP9X leads to the deubiquitination of APC_C. 1 / 1
| 1
reach
"Because USP9X is a DUB and the SAC signal relies on inhibition of the APC/C ubiquitin ligase, USP9X might antagonize APC/C dependent ubiquitination to help maintain the SAC."
29669289
USP9X affects ANK3
| 1
USP9X deubiquitinates ANK3. 1 / 1
| 1
reach
"USP9X deubiquitination of ANK3 (and other synaptic proteins including ANK2, SHANK3, and TNKS2) is required to antagonize its proteasomal degradation and is essential for synapse formation (30)."
35662507
USP9X affects ALKBH3
| 1
USP9X deubiquitinates ALKBH3. 1 / 1
| 1
reach
"Instead, it seemed to act as a scaffold protein, recruiting two further DUBs, USP7 and USP9x, which then de-ubiquitylated ALKBH3."
26588485
USP9X affects ALDH1A3
| 1
USP9X deubiquitinates ALDH1A3. 1 / 1
| 1
reach
"USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells."
30958800