IndraLab

"Furthermore, BAP1 also deubiquitinates its main interacting protein, host cell factor 1 (HCF-1) [107] ."

"Machida et al. reported that HCFC1 was deubiquitinated by the deubiquitinating enzyme BRCA1-associated protein 1 to regulate cell divisions and proliferation 39."

"10 Deubiquitination of HCF1 and H2Aub by BAP1 promotes their association with EF2, YY-1, and FoxK2, which regulate cell-cycle progression, cell proliferation and cell-cycle control."

"It has been proposed that BAP1 may modulate cell proliferation by deubiquitylating the transcriptional regulator HCF1."

"BAP1 also promoted cell proliferation through directly interacting and deubiquitinating host cell factor-1 (HCF-1) [12] ."

"HCF-1 is modified with ubiquitin in vivo, and ectopic studies suggest BAP1 deubiquitinates HCF-1."

"BAP1 also interacts with and deubiquitinates the transcriptional regulator host cell factor 1 (HCF1)."

"Moreover, BAP1 modulates histone H2A and HCFC1 deubiquitination and regulates transcription, cell cycle and growth, response to DNA damage and chromatin dynamics."

"BAP1 can deubiquitinate HCF-1 and is crucial for HCF-1 activity ( Zhou et al., 2013 )."

"It has been proposed that ubiquitylation of HCF1 blocks E2F responsive promoter activity, and that HCF1 deubiquitylation by BAP1 would remove this inhibition and promote cell proliferation 33."

"BAP1 deubiquitinates poly-ubiquitinated HCF-1, yet depletion of BAP1 has shown mixed effects on the stability of HCF-1 protein levels [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."

"Additionally, BAP1 deubiquitinates the lysine residues of HCF-1 to recruit multiprotein complex partners (i.e. OGT and PGC1α) and stabilize these partners, thereby potentiating transcriptional regulatory effects (49)."

"BAP1 deubiquitinates H2Aub."

"In conclusion, we found that BAP1 can deubiquitinate H2Aub and induces radioresistance in HNSCC cells via modulation of HR."

"Other studies in mammalian cells have also shown that BAP1 represses the expression of some target genes with BAP1 dependent deubiquitination of H2Aub on the corresponding gene promoters XREF_BIBR, XREF_BIBR."

"BAP1 deubiquitinates histone 2A (H2Aub) on the SLC7A11 promoter and represses SLC7A11 expression in a p53-independent way (39, 40)."

"At the SLC7A11 promoter, BAP1 deubiquitinates H2Aub and subsequently inhibits the expression of SLC7A11."

"10 Deubiquitination of HCF1 and H2Aub by BAP1 promotes their association with EF2, YY-1, and FoxK2, which regulate cell-cycle progression, cell proliferation and cell-cycle control."

"Although USP16 is clearly demonstrated to facilitate transcriptional regulation in this manner, BRCA1-associated protein 1 (BAP1), a member of the UCH family of DUBs, can also deubiquitinate H2Aub and regulate transcription, implying a degree of redundancy [66,67]."

"These results suggest that HG inhibits SLC7A11 function by promoting BAP1 expression, thereby inducing ferroptosis in podocytes.3.5 HG promotes ferroptosis by promoting BAP1 deubiquitination of H2Aub on SLC7A11."

"BAP1 has been shown to induce deubiquitination of H2Aub on the SLC7A11 promoter."

"According to a report by Zhang et al., BAP1 causes the deubiquitination of H2Aub, which prevents SLC7A11 from being expressed [14]."

"Studies have revealed that BAP1 is activated by ASXL1 and can selectively deubiquitinate the H2Aub K119 site, which is involved in regulating cellular physiological activities and various cell damage modes [44]."

"Compared with GST and BAP1-C91S, BAP1 specifically decreased KLF5 polyubiquitination (XREF_FIG; XREF_SUPPLEMENTARY)."

"For instance, BAP1 deubiquitinates and stabilizes the transcription factor KLF5, which is highly expressed in breast cancer and a potent biomarker for unfavorable prognosis in breast cancer patients, and promotes breast cancer cell proliferation, survival, and migration as well as tumor growth ."

"<span class="match term0">BAP1</span> interacts directly with <span class="match term1">KLF5</span> and stabilizes <span class="match term1">KLF5</span> via deubiquitination"

"In breast cancer cell lines, BAP1 plays an oncogenic function by directly deubiquitinating and stabilizing KLF5 (Kruppel-like factor 5)."

"BAP1 has been reported to promote breast cancer cell proliferation and metastasis by deubiquitinating KLF5 [ 18 ]."

"BAP1, but not BAP1-C91S, markedly decreased KLF5 protein polyubiquitination (XREF_FIG, lanes 3 and 4), whereas another DUB, A20, did not decrease KLF5 polyubiquitination (XREF_FIG, lane 5)."

"Finally, we demonstrated that knockdown of endogenous BAP1 increased the ubiquitination of endogenous KLF5 in HCC1806 (XREF_FIG)."

"Second, BAP1 decreases KLF5 polyubiquitination and increases KLF5 protein stability in a DUB activity dependent manner."

"Notably, KLF5 is de-ubiquitylated by BRCA1 associated protein-1 (BAP1) (Jia et al. 2021; Qin et al. 2015) and suppresses autophagy by activating the PI3K-AKT-mTOR signaling (Jia et al. 2021) that can inhibit the autophagy initiation and the autophagosome formation via modulating the activity of AMBRA1 and ULK1 complex (Xu et al. 2020; Nazio et al. 2013)."

"At the SLC7A11 promoter, BAP1 deubiquitinates H2Aub and subsequently inhibits the expression of SLC7A11."

"BAP1 deubiquitinates histone 2A (H2Aub) on the SLC7A11 promoter and represses SLC7A11 expression in a p53-independent way (39, 40)."

"BAP1 represses solute carrier family 7 member 11 (SLC7A11) expression by reducing H2A ubiquitination of the SLC7A11 promoter, resulting in lipid peroxidation and ferroptosis (Zhang et al., 2019)."

"Given that miR-31-5p has been shown to reduces DOX-induced cardiac toxicity [16], we investigated the role of the targeted relationship between miR-31-5p and BAP1 in SCM.Against this background, it is clear that the regulation of SLC7A11 deubiquitination by BAP1 may have an impact on ferroptosis in SCM."

"To determine whether BAP1 induced the deubiquitination of SLC7A11, the deubiquitination enzyme inhibitor G5 was added to examine its effect on the ubiquitination level of SLC7A11."

"These findings imply that BAP1 induces the deubiquitination of SLC7A11, thereby inhibiting its expression."

"As shown above, BAP1 promotes the deubiquitination of SLC7A11, leading to ferroptosis in H9C2 cells."

"BRCA1-associated protein 1 (BAP1) decreases SLC7A11 promoter H2A ubiquitination via an H2A deubiquitinase that functions as a tumor suppressor."

"At the ER, BAP1-dependent deubiquitination of the type 3 inositol-1,4,5-trisphosphate receptor (IP3R3) is necessary to sustain the IP3R3 ER channel integrity (11,55)."

"We hypothesized that BAP1 might deubiquitylate and stabilize IP3R3."

"For example, BAP1 promotes apoptosis by deubiquitinating and stabilizing IP3R3 at the endoplasmic reticulum (ER), which stimulates Ca release from the ER into the cytosol and thereby increases the mitochondrial Ca concentration and cytochrome C release ."

"BAP1 can regulate Ca release from the ER to the cytoplasm and mitochondria to promote cell apoptosis by binding, deubiquitylating and stabilizing IP3R3 [45]."

"Along with maintenance of IP3R3 ER channel integrity, BAP1-dependent deubiquitination of IP3R3 may change the threshold for IP3R3 activation by Ca itself or IP3."

"BAP1 regulates Ca release from endoplasmic reticulum and mitochondria by deubiquitinating and stabilizing inositol 1,4,5-trisphosphate receptor type 3 (IP3R3), promoting apoptosis after genotoxic stress (14)."

"The endoplasmic reticulum (ER) fraction is the primary location of cytoplasmic BAP1, where it deubiquitylates and stabilizes the type 3 inositol-1,4,5-trisphosphate receptor (IP3R3)."

"BAP1 (C91S), despite being able to bind IP3R3 (XREF_FIG), failed to deubiquitylate IP3R3 (NT) (XREF_FIG and XREF_FIG), underscoring the requirement of BAP1 catalytic activity."

"Another study showed de-ubiquitination of gamma-tubulin by BAP1 prevents chromosome instability in breast cancer cells [15]."

"20 Additionally, loss of BAP1 or its DUB activity reduces deubiquitination of Gamma-tubulin in breast cancer cell lines, which may be attributed to the perturbation of mitotic spindle fiber formation [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"BAP1 can deubiquitinate γ-tubulin [32], as well as microspherule protein 1 (MCRS1), a protein that caps the minus ends of mitotic microtubules to prevent their depolymerization [33, 34]."

"While the mechanism remains to be fully elucidated, it seems that deubiquitylation of gamma-tubulin by BAP1 during mitosis allows proper spindle organisation and function [XREF_BIBR]."

"Deubiquitination of gamma-tubulin by BAP1 prevents chromosome instability in breast cancer cells."

"A recent study has linked the deubiquitination of gamma-tubulin by BAP1 to breast cancer."

"BAP1 overexpression also abolished basal Ino80 ubiquitination (XREF_FIG and XREF_FIG, rightmost lanes)."

"BAP1 deubiquitylates INO80 and thereby protects the protein from ubiquitin mediated proteolysis."

"BAP1 interacts with and deubiquitylates INO80, playing a critical role in stabilizing and targeting the INO80 chromatin remodeling complex to the replication forks."

"In line with this notion, BAP1 depletion resulted in reduced localization of RPA, RAD51 and BRCA1 to IRIF or microlaser irradiation site [100,101], although it remains ambiguous whether either histone[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Since BAP1 deubiquitinates and stabilizes Ino80, we investigated the relationship between CHIP and BAP1 in Ino80 ubiquitination."

"In addition to MCRS1, a recent study revealed that BAP1 deubiquitinates and stabilizes Ino80, a key subunit of the INO80 chromatin remodeling complex implicated in DNA replication during stress."

"BRCA1-associated protein 1 (BAP1) [18], the crucial constituent of the deubiquitinase complex, attenuated the initiation and extension of SLC7A11 transcription by deubiquitinating histone 2A, while the steady-state levels and half-life of SLC7A11 were improved by combination with the ovarian tumor family member deubiquitinase (OTUB1) [19]."

"BAP1 deubiquitinates histone 2A (H2Aub) on the SLC7A11 promoter and represses SLC7A11 expression in a p53-independent way (39, 40)."

"The mechanism may be that BAP1 represses the transcription of SLC7A11 by reducing histone 2A ubiquitination (H2Aub) on the SLC7A11 gene (49)."

"BAP1 deubiquitinates histone 2A in the regulatory chromatin of the of SLC7A11 gene, which encodes, a key transporter of extracellular cysteine."

"Specially, previous studies demonstrate that BAP1 downregulates SLC7A11 expression by reducing histone 2A ubiquitination occupancy on its promoter (Zhang et al., 2018b)."

"Histone 2A (H2A) at Lysine 119 was deubiquitinated by BAP1."

"p53 binds to the SLC7A11 promoter, directly suppressing its transcription; the nuclear deubiquitinase (DUB) BAP1 represses SLC7A11 transcription by removing histone 2A ubiquitination from the SLC7A11 promoter; and KEAP1 represses SLC7A11 transcription through degrading NRF2, a master transcription factor of antioxidant response and regulator of SLC7A11."

"Recently, it has been found that BAP1 can inhibit the expression of SLC7A11 to suppress the uptake of cystine by deubiquitinating histone 2A on the SLC7A11 promoter."

"Further mechanistic studies demonstrated that BAP1 suppresses SLC7A11 expression partly by deubiquitinating histone 2A ubiquitination to promote ferroptosis."

"BRCA1 associated protein 1 (BAP1) is a ubiquitin C-terminal hydrolase that deubiquitinates histone H2AK119ub and other proteins and regulates the expression of multiple genes."

"Mechanistically, BAP1 deubiquitinates histone 2AK119-ub1 (H2AK119-ub1), an epigenetic mark that represses gene expression and can have long-lasting effects on transcription."

"Recent studies indicated epigenetic modifications as a result of deubiquitinating the mono-ubiquitinated H2AK119 histone by the hyperactivated BAP1/ASXL1 is one of the main drivers of the transcriptio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"cBioPortal functional prediction and our structural, biochemical, and cellular studies indicate all these mutations lead to a loss of function where BAP1/ASXL1 fails to effectively deubiquitinate H2AK119Ub, and thus causes aberrant accumulation of this chromatin modification."

"Our data support a model where BAP1/ASXL1 deubiquitinates H2AK119Ub chromatin by anchoring to the nucleosomal acidic patch, DNA dyad, and the DNA exit regions using conserved arginine and lysine tracts."

"These results further provide a molecular explanation for how >50 mutations in BAP1 and ASXL1 found in cancer can dysregulate H2AK119Ub deubiquitination, providing insight into understanding cancer etiology."

"BAP1 deubiquitinates H2AK119Ub, regulating chromatin packing and gene expression (Scheuermann et al., 2010; Foglizzo et al., 2018)."

"Our data support a model where BAP1/ASXL1 deubiquitinates H2AK119Ub chromatin by anchoring to the nucleosomal acidic patch, DNA dyad, and the DNA exit regions using conserved arginine and lysine tracts."

"As is the case with the loss-of-function model, these data suggest that mutant ASXL1 inhibits PRC2 function, followed by upregulation of its target genes in a dominant-negative manner, which contribut[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"To investigate whether BAP1 can deubiquitylate and stabilize OGT directly, rather than decreased OGT being secondary to a reduction in HCF-1, we affinity purified ubiquitylated and Myc tagged human OGT from HEK293T cells, and then incubated it with human BAP1 and human ASXL1 residues 2-365 co-purified from Sf9 cells."

"The tumor suppressor <span class="match term0">BAP1</span> associates with ASXL1/2 to form the core Polycomb complex PR-DUB, which catalyzes the removal of mono-ubiquitin from several substrates including histone H2A. This complex also mediates the poly-deubiquitination of HCFC1, <span class="match term1">OGT</span> and PCG1-alpha, preventing them from proteasomal degradation."

"OGT is a well-known protein associated with BAP1 in the BAP1 core complex, which include ASXL1, ASXL2, HCF1, OGT, and the forkhead transcription factors FOXK1 and FOXK2 [9], and BAP1 can deubiquitinate and stabilize OGT [26]."

"XREF_BIBR, XREF_BIBR, XREF_BIBR BAP1 also deubiquitylates OGT and regulates its levels."

"BAP1 deubiquitinates and stabilizes OGT [75], while CDK1 phosphorylates KIF18A during mitosis at S674/S684."

"<span class="match term0">BAP1</span> also controls G1/S cell cycle progression by regulating <span class="match term1">BRCA-1</span> [111], Ying Yang 1 (YY-1), and host cell factor 1 (HCF-1)"

"Therefore, we opine that the BAP1/BRCA1 interaction inactivates the NF-κB signaling to protect septic AKI.In conclusion, this study demonstrates that BAP1 protects mice from sepsis-induced AKI by deub[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"We hypothesized that BAP1 might deubiquitinate BRCA1 and protect it from proteasome mediated degradation; this might explain the BRCA1 stabilization by the mutant BAP1 forms that retained the DUB activity."

"As a possible mechanism of BRCA1 stabilization by BAP1 transduction in this study, we speculated that the ubiquitination of BRCA1 protein might be directly deubiquitinated by BAP1."

"3 Nuclear BAP1 indeed deubiquitylates BRCA1 associated RING domain 1 (BARD1), which in a heterodimer with BRCA1, forms the E3 ubiquitin ligase that regulates the DNA damage response (DDR)."

"Taken together, these data suggest that the BAP1 is likely an upstream DUB of MYCN to deubiquitinate and stabilize MYCN protein."

"E3 ubiquitin ligases such as FBXW7 could promote MYCN ubiquitination and degradation, however, BAP1 deubiquitinates MYCN and increases its stability."

"Inhibiting BAP1 by shRNA-mediated knockdown decreases the expression of MYCN largely through reducing the BAP1-mediated deubiquitination of MYCN."

"Notably, BAP1 could reduce the polyubiquitination of MYCN (Fig. 1I), which might be the underlying mechanism for the effect of BAP1 on extend half-life of MYCN."

"BAP1 expression reduced the ubiquitination of GFP-ERK2, indicating that BAP1 deubiquitinates ubiquitins on GFP-ERK2 (Fig. 4D)."

"Moreover, BAP1 was found to control MSC migration through the ERK signaling pathway, with BAP1 deubiquitinating ubiquitins on phosphorylated-ERK1/2."

"BAP1 deubiquitinates ubiquitins on phosphorylated-ERK1/2 to suppress MSC migration ."

"Deubiquitination of SMN by Bap1.."

"Notably, overexpression of Bap1 reversed the ubiquitination of SMN (Figure 7D), and this result indicated that Bap1 could regulate SMN levels by acting as a deubiquitinating enzyme."

"While Bap1 could prevent the accumulation of ubiquitinated SMN in the UPS-inhibited cells, Bap1 was unable to deubiquitinate SMN (Figure 7E)."

"BAP1 deficiency enhanced cancer cell proliferation and lipid synthesis via the LKB1-AMPK-mTOR signalling pathway.In conclusion, we suggest that BAP1 interacts with and deubiquitinates LKB1, thereby af[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Altogether, this work has revealed a previously unknown mechanism for how BAP1 suppresses tumour through deubiquitinating LKB1."

"<span class="match term0">BAP1</span> regulates AMPK-mTOR signalling pathway through deubiquitinating and stabilizing tumour-suppressor <span class="match term1">LKB1</span>"

"However, another study suggests that BAP1 may deubiquitinate and stabilize PTEN, leading to Akt inhibition [133]."

"Mechanistically, <span class="match term0">BAP1</span> complexed with <span class="match term1">PTEN</span> and stabilized <span class="match term1">PTEN</span> via deubiquitination and, furthermore, negatively regulated HCC cell EMT by deactivating the AKT/GSK-3beta/Snail pathway"

"BAP1 can physically bind to and deubiquitinate PTEN, which inhibits the ubiquitination mediated degradation of PTEN and thus stabilizes PTEN protein."

"Moreover, de-ubiquitination of MCRS1 by BAP1 plays important roles in regulating proper mitotic progression and prevents chromosome instability.The yeast two-hybrid screen was performed with full leng[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Since BAP1 is a de-ubiquitinating enzyme, we explored whether BAP1 de-ubiquitinates and stabilizes MCRS1."

"BAP1 can deubiquitinate γ-tubulin [32], as well as microspherule protein 1 (MCRS1), a protein that caps the minus ends of mitotic microtubules to prevent their depolymerization [33, 34]."

"In the present study, we have identified that BAP1 deubiquitinates KEAP1 by binding to the BTB domain."

"Together, our study demonstrates that KEAP1 deubiquitination by BAP1 is novel tumor suppressive mechanism of LUAD."

"BAP1 Downregulates NRF2 Target Genes and Exerts Anti-Tumorigenic Effects by Deubiquitinating KEAP1 in Lung Adenocarcinoma."

"Mammalian BAP1 deubiquitylates H2AK119ub1, 87 and BAP1 reintroduction into BAP1 deficient ccRCC cell lines affected global levels of H2AK119ub1."

"XREF_BIBR reported that BAP1 is phosphorylated at serine 592 following DNA damage, that phospho-BAP1 causes deubiquitination of H2AK119ub1, and that this signal promotes the HR pathway."

"87 As Drosophila Calypso, human BAP1 deubiquitylates H2AK119ub1."

"Functionally, BAP1 is a nuclear deubiquitinating (DUB) enzyme belonging to the ubiquitin C-terminal hydrolase DUB subfamily."

"BAP1 can encode a nuclear deubiquitinating (DUB) enzyme that participates in the composition of the polycomb repressive deubiquitinase (PR-DUB) complex."

"BAP1 belongs to the deubiquitinate (DUB) family of proteins and acts as a ubiquitin carboxy-terminal hydrolase (UCH)."

"Moreover, BAP1 was found to control MSC migration through the ERK signaling pathway, with BAP1 deubiquitinating ubiquitins on phosphorylated-ERK1/2."

"BAP1 deubiquitinates ubiquitins on phosphorylated-ERK1/2 to suppress MSC migration ."

"Our results suggest reciprocal positive interactions between BAP1 and DNA-PKcs, based on phosphorylation of BAP1 by the latter and deubiquitination of DNA-PKcs by BAP1."

"Activated DNA-PK phosphorylates BRCA1-associated protein 1 (BAP1) at S395, and BAP1, in turn, deubiquitinates DNA-PKcs, establishing a positive feedback loop between the two (Sato et al., 2024)."

"However, this is unlikely to account for the stabilization of PGC-1alpha by OGT because 1) the S333A mutation, which bypasses the effects of O GlcNAcylation on the proteasome, increases PGC-1alpha ubiquitination and decreases its stability, 2) HCF-1 and BAP1 have no effect on proteasome function or global ubiquitination (XREF_FIG and data not shown), but can decrease PGC-1alpha ubiquitination and increase its stability."

"Overexpression of BAP1 does not increase global ubiquitination, but substantially increases the level of PGC-1alpha and decreases PGC-1alpha ubiquitination as normalized to the protein level (XREF_FIG)."

"Loss of Asx in flies led to an increase in Ub-H2A levels without influencing other chromatin marks (H3K4 me3, H3K27me3), and assays using purified proteins found Asx stimulates Calypso activity towards Ub-AMC, and that Asx and Calypso and the human orthologs BAP1 and ASXL1 deubiquitinate H2A but not H2B in reconstituted nucleosomes [XREF_BIBR]."

"Subsequent WB using anti-HA and anti- Flag antibodies showed that the BAP1 WT, but not a MT, deubiquitinated H2B in vivo."

"28 ASXL1 may also collaborate with BAP1, loss of which led to a myelodysplastic syndrome like syndrome in a mouse model, to deubiquitinate H2AK119 at polycomb group targets."

"BAP1 is activated by ASXL1 and deubiquitylates mono-ubiquitylation of H2AK119 (Daou et al. 2015; Sahtoe et al. 2016)."

"BRCA1 forms a complex with BARD1 to generate an active E3 ubiquitin ligase that can auto-ubiquitinate to promote its own destruction, while BAP1 is a DUB that by deubiquitinating BARD1 can disrupt this complex and reduce its turnover [12, 15]."

"BAP1 also deubiquitinates BARD1 and modulates the BRCA1-BARD1 complex during DNA damage response [ 14 ]."

"It has been proposed that BAP1 may modulate cell proliferation by deubiquitylating the transcriptional regulator HCF1."

"For example, BAP1 promotes apoptosis by deubiquitinating and stabilizing IP3R3 at the endoplasmic reticulum (ER), which stimulates Ca release from the ER into the cytosol and thereby increases the mitochondrial Ca concentration and cytochrome C release ."

"Furthermore, BAP1 deubiquitylates the deubiquitylase adaptor module DEUBAD of ASXL2, leading to its stabilization."

"<span class="match term0">BAP1</span> also controls G1/S cell cycle progression by regulating BRCA-1 [111], Ying Yang 1 (YY-1), and host cell factor 1 (HCF-1)"

"The association between <span class="match term0">BAP1</span> and <span class="match term1">14-3-3</span> protein releases the apoptotic inducer protein Bax from <span class="match term1">14-3-3</span> and promotes cell death through the intrinsic apoptosis pathway"

"However, this might not remain true in malignancies in which the VHL gene (34)—or other genes that suppress HIF-1α (10)—are also mutated and thus display constitutively high levels of HIF-1α, which may overrun the fine-tuning BAP1 deubiquitylating activity.In addition to VHL, which is active in normoxia, other proteins mediate the ubiquitylation of HIF-1α in hypoxia."

"Deubiquitination of gamma-tubulin by <span class="match term0">BAP1</span> prevents chromosome instability in breast cancer cells"

"Similarly, the active moiety of tumor suppressor BAP1 could also repress SCL7A11 expression by deubiquitinating H2A at the SLC71A11 promoter to regulate ferroptosis [30]."

"Downstream of ASXN is the ASX homology (ASXH) domain (also known as the DEUBAD domain) encoded by exons 9-11, which participates in interactions with epigenetic regulatory proteins, including the BRCA1 Associated Protein 1 (BAP1) deubiquitinating protease [XREF_BIBR - XREF_BIBR]."

"We find, however, BAP1 does not deubiquitinate OMM proteins in osteoclasts, as established by VDAC co-IP using ubiquitin and VDAC antibodies, even in the presence of the proteasomal inhibitor, MG132 (Supplementary Fig. 3c)."

"The theoretical hypothesis is presented in XREF_FIG : Mutant Bap1 protein continues to deubiquitylate ubiquitous Notch signaling complexes, which are supposed to be degraded after ubiquitylation."

"<span class="match term0">BAP1</span>, a deubiquitinating enzyme (DUB), was further identified as a positive N1ICD regulator, and NUMB facilitates the association between N1ICD and <span class="match term0">BAP1</span> to stabilize N1ICD"

"shRNA knockdown of BAP1 increased NCoR1 ubiquitination and significantly reduced the recruitment of NCoR1 to sites within the globin locus that could be rescued by proteasome inhibitor treatment, indicating that this deubiquitinase plays an important role in NCoR1 activity and therefore, in turn, DRED complex regulation."

"BAP1 expression reduced the ubiquitination of GFP-ERK2, indicating that BAP1 deubiquitinates ubiquitins on GFP-ERK2 (Fig. 4D)."

"BAP1 as a nuclear deubiquitinating enzyme (DUB), bound to and deubiquitinated MAFF, thereby stabilizing it."

"BAP1 suppresses white adipose tissue browning and thermogenesis through deubiquitinating KDM1B."

"Since K119 and K13/15 reside at distinct locations in the nucleosome (XREF_SUPPLEMENTARY) we asked whether BAP1 could also deubiquitinate K13/15."

"The calypso and BAP1 DUB deubiquitylates K119ub on histone H2A, leading to gene repression."

"BAP1 suppresses RCC cell proliferation and deubiquitinates H2Aub1 in renal cancer cells."

"28031783"

"Mechanistically, BAP1 deubiquitinates histone 2AK119-ub1 (H2AK119-ub1), an epigenetic mark that represses gene expression and can have long-lasting effects on transcription."

"BRCA1 associated protein1 (<span class="match term0">BAP1</span>) is a tumor suppressor involved in multiple cellular processes such as transcriptional regulation, chromatin modification by deubiquitinating <span class="match term1">histone 2A</span>, and DNA repair"

"30202049"

"28031783"

"However, it is unclear whether BAP1 participates in the deubiquitination of FOXO3a protein."

"BRCA1-associated protein 1 (<span class="match term0">BAP1</span>) deubiquitinase antagonizes the ubiquitin-mediated activation of <span class="match term1">FoxK2</span> target genes. Okino Y(1), Machida Y(1), Frankland-Searby S(2), Machida YJ(3). Author information: (1)From the Departments of Oncology and. (2)Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905. (3)From the Departments of Oncology and Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905 machida.yuichi@mayo.edu. BRCA1-associated protein 1 (<span class="match term0">BAP1</span>), which is frequently mutated in cancer, functions as a deubiquitinase (DUB) for histone H2A.<span class="match term0">BAP1</span> represses <span class="match term1">FoxK2</span> target genes, and this effect requires <span class="match term0">BAP1</span> DUB activity but not interaction with HCF-1"

"A recent study indicated that BAP1 and its Drosophila homolog Calypso deubiquitinate H2AK119Ub, with minimal activity against H2BK120Ub or polyubiquitin chains (Scheuermann et al., 2010)."

"Mammalian BAP1 deubiquitylates H2AK119ub1, 87 and BAP1 reintroduction into BAP1 deficient ccRCC cell lines affected global levels of H2AK119ub1."

"Furthermore, BAP1 deubiquitylates the deubiquitylase adaptor module DEUBAD of ASXL2, leading to its stabilization."

"Additionally, BAP1 modulates intracellular Ca signalling by deubiquitinating (and stabilizing) inositol 1,4,5-trisphosphate (IP3) receptors, prominent Ca release channels in the ER [117]."