IndraLab

Statements

USP7 affects TP53
2 2 29 2 1 | 13 1 351 186
USP7 binds TP53.
29 2 | 1 92 181
TP53 binds USP7. 10 / 235
29 2 | 1 77 105
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"Surprisingly, however, we have found that direct interaction between HAUSP and p53 is not absolutely required for it to antagonize efficiently Mdm2 mediated ubiquitination of p53 and that HAUSP is capable of enzymatically functioning in trans on p53 by using Mdm2 as a bridge."
17525743
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"The results of the present study were consistent with these previous findings, demonstrating that the deletion and inhibition of HAUSP was associated with p53 upregulation ( xref )."
27899971
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"USP7 interacts and regulates p53 as well as MDM2 stability by promoting their deubiquitination (Li et al., 2002; Ma et al., 2010)."
30067985
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"The N-terminal region of Bat3 containing an ubiquitin-linked domain was found to have capacity to form a stable complex with both HAUSP ( xref , lane 3) and p53 ( xref , lane 3), but other deletions, which are lacking the N-terminus, completely abolish the ability to form complexes ( xref , lanes 4 and 5; xref , lanes 5 and 6)."
31647545
sparser
"Intriguingly, Becker et al. described that the hyperubiquitylation of p53 contributes to its aberrant cytoplasmic retention in neuroblastoma in association with the impaired interaction between p53 and HAUSP which catalyzes the deubiquitylation of p53 [ xref ]."
21188172
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"Moreover, USP7 can interact with p53 and promote its expression through mediating its deubiquitination."
35538032
sparser
"Interestingly, STIP knock down seem to have a more profound impact on the interaction between USP7 and Mdm2 than the interaction between USP7 and p53."
26460617
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"The interactions between HAUSP and p53 or MDM2 are likely to be more complex in vivo, not only due to the presence of multiple binding sites in MDM2 but also because of the oligomeric nature of p53 and possibly HAUSP."
16402859
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"Consistent with this, our results reveal that STIP knock down have a more profound impact on the interaction between USP7 and Mdm2 than the interaction between USP7 and p53."
26460617
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"As efforts to obtain crystals of the p53 and HAUSP complex were not successful, they generated protein chimeras -- made of half p53 peptides and half HAUSP TRAF like domain -- to determine the complex 's structure and mechanism of binding."
20076526
TP53 binds USP7 and MDM2. 10 / 51
| 13 38
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"In addition, USP7 can interact indirectly with p53, using Mdm2 as a bridge, resulting in the formation of USP7-Mdm2-p53 complexes."
26460617
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"The HAUSP, p53, and Mdm2 complex and p53-nucleolin interplay in DNA damage have been identified XREF_BIBR XREF_BIBR XREF_BIBR; however, the molecular relation between these two findings was poorly understood."
26238070
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"Taken together, the activation of USP7-MDM2-p53 interaction can promote the occurrence and development of tumors."
31114498
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"P53, as a tumour-suppressor, exerts an essential role in signalling associated with the control of cell survival and aberrant p53 signalling results in unchecked cell growth and the initiation of cancer.USP7 interacts with a range of protein targets in the p53 pathway (p53, MDMX, MDM2)39 and MDM2 plays a key role in p53 stabilization by increasing the ubiquitination process.39 USP7 deubiquitinates MDM2 and its functional regulator MdmX and stabilizes p53.39 USP7 apparently has 2 independent p53 regulatory functions, stabilizing p53 through deubiquitylation and regulating the sequence-specific DNA binding of p53.40 Within the USP7, MDM2 and p53 complex, p53 and the MDM2 have been shown to bind USP7 through the TRAF domain.39 Under normal conditions, USP7 prefers MDM2 as a substrate rather than p53 and MDM2 has a higher binding affinity for USP7 compared to p53.41 Reduction of USP7 expression levels leads to p53 stabilisation, and destabilization of MDM2."
35005106
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"The authors find that genotoxic stress induces a shift in complex formation from a p53, MDM2, and USP7 complex to p53/GMPS/USP7."
24507714
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"Binding of HAUSP to either p53 or MDM2 leads to their de-ubiquitination, and binding to MDM2 leads to p53 destabilization due to increased MDM2 stability [ xref ]."
19538162
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"USP7 can bind directly to Mdm2 or p53 in a mutually exclusive manner."
26460617
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"Although USP7 can interact with both Mdm2 and p53 depending on the cellular context, USP7 preferentially forms a stable USP7-Mdm2 complex even in the presence of excess p53 [ xref ], indicating that USP7 predominantly functions to stabilize Mdm2."
33207738
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"However, the linkage between the DNA damage response machinery and the HAUSP, p53, and Mdm2 complex in DNA damaged cells still remain unresolved."
26238070
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"Taken together, this study reveals a new component of the HAUSP, p53, and Mdm2 complex that governs dynamic cellular responses to DNA damage."
26238070
TP53 binds USP10 and USP7. 3 / 3
| 3
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"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."
31745110
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"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."
30067985
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"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30067985
TP53 binds USP7 and BAG6. 3 / 3
| 3
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"Notably, we revealed that a p53–HAUSP–Bat3 trimeric protein complex could exist in vivo with HAUSP serving as a binding mediator for enhanced interaction between p53 and Bat3, which antagonizes the interaction of p53 with its proteasome receptor S5a and promotes the accumulation of p53 in the nucleus."
31647545
sparser
"These results suggest that the formation of p53–HAUSP–Bat3 complex may prevent p53 from being recognized and binding to its proteasome receptor S5a, subsequently leading to the accumulation and stabilization of p53 in nucleus ( xref ) though we still do not know whether there is conformational change in Bat3 and p53 at present."
31647545
sparser
"Immunoprecipitation of protein extracts with anti-p53 antibody and subsequent immunoblot analysis revealed a trimeric protein interaction between p53, Bat3 and HAUSP as both HA-Bat3 and Flag-HAUSP were detected in the p53-immunoprecipitated complex ( xref )."
31647545
TP53 binds USP7 and EBNA1. 3 / 3
| 3
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"Crystal structure analysis showed that USP7 binds to its substrate p53 and its inhibitory interactor Epstein–Barr nuclear antigen 1 (EBNA1) protein through the same pocket but the former binding partner p53 exhibit weaker contacts with USP7 [ xref , xref ]."
28031783
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"NMR studies of USP7 bound by EBNA1 and p53 indicated that p53 and EBNA bind the same pocket but p53 but makes less extensive contacts with USP7."
19489724
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"The results of these experiments demonstrated that the deletion of Ubl2 did not inhibit the interaction of USP7 with p53 or EBNA1 ( xref , A and B )."
32587091
TP53 binds USP7 and GMPS. 2 / 2
| 2
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"In the context of X-ray radiation, GMPS bound both USP7 and TP53 (Fig. xref b), and TP53 protein levels were elevated upon GMPS overexpression (Additional file xref : Figure S1e), suggesting that GMPS promotes TP53 protein stability."
32005234
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"Considering that the interaction among GMPS, USP7 with p53 is required for the deubiquitination and stabilization of p53 [ xref , xref ]. we next determined the interaction of GMPS and USP7 with p53 in the p53wt breast cancer cell lines MCF-7 and DU4475 by co-IP assays."
33742136
TP53 binds USP7, WRAP53, and MDM2. 2 / 2
| 2
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"In this study, we report a new molecular process in which WDR79 interacts with USP7 to modulate the stability of Mdm2 and p53, which promotes the proliferation of NSCLC cells."
28406480
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"Taken together, our findings reveal a novel role of WDR79 in the proliferation of NSCLC cells and could pinpoint a new mechanism by which WDR79 and USP7 functionally interact to modulate the Mdm2-p53 pathway."
28406480
TRAF binds TP53 and USP7. 2 / 2
| 2
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"As shown in xref , p53 has 393 amino acid residues that can be subdivided into five domains: the N-terminal transactivation domains (TAD) that are responsible for its binding to the p53-binding sites on MDM2 and MDMX, a proline-rich region (PP) that contains five PxxP motifs and is essential for inducing apoptosis, a DNA-binding domain (DBD), a tetramerization domain (TET), and a C-terminal domain (CTD) that is critical for the binding of p53 to the TRAF domain of USP7 ( xref ; xref )."
32300595
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"On one hand, p53 binds to TRAF domain and C-terminal (amino acids 880–1050) of USP7, and then USP7 ubiquitinates p53 directly and prevents it from degradation."
31114498
TP53 binds USP7, DAXX, and MDM2. 2 / 2
| 1 1
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"The most significant binding partners identified to interact with the C1 domain are the TNF-R1 and TRAIL-R1 -- Modulator of Apoptosis-1 (MOAP-1) complexes and the MDM2, DAXX, HAUSP, and p53 complex [XREF_BIBR, XREF_BIBR] (XREF_FIG)."
22577550
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"Although the molecular mechanism for the interactions of DAXX, HAUSP, Mdm2, and p53 underlying this regulatory pathway are largely unknown, an important clue is provided by our observation that, at le[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21134643
TP53 binds USP7, ELP2, and MDM2. 2 / 2
| 2
sparser
"Considering that the effect of STIP on Mdm2 or p53 was independent of each other, we hypothesize that STIP may associate with the USP7-Mdm2 or USP7-p53 complexes."
26460617
sparser
"These data indicated that STIP may simultaneously bind to USP7 and either Mdm2 or p53, mediating the assembly of ternary STIP-USP7-Mdm2 and STIP-USP7-p53 complexes, respectively."
26460617
TP53 binds USP7, MDM2, and NCL. 2 / 2
| 2
sparser
"We next verified that HAUSP and nucleolin could form a complex with p53 and Mdm2 by examining the interaction between HAUSP and nucleolin in p53- and Mdm2-depleted cells."
26238070
sparser
"Moreover, p53 also could not interact with HAUSP and nucleolin in Mdm2-depleted cells ( xref )."
26238070
TP53 binds USP7 and ABRAXAS2. 2 / 2
| 2
sparser
"Based on the results showing association of ABRO1, p53 and USP7, we presumed that ABRO1 might promote interaction between USP7 and p53, and regulate USP7-dependent deubiquitination of p53."
25283148
sparser
"ABRO1 forms a complex with USP7 and p53."
25283148
TP53 binds USP7 and NCL. 1 / 1
| 1
sparser
"We hypothesized that Mdm2 acts as a mediator between HAUSP and nucleolin in the normal state, and that p53 may be associated with the HAUSP and nucleolin interaction in the DNA damaged condition."
26238070
TP53 binds USP7 and MDM4. 1 / 1
| 1
sparser
"It has also been reported that some SNPs of p53, Mdm2(Murine double minute 2), MdmX and Hausp (Herpes virus-associated ubiquitin-specific protease) in p53 pathway are associated with the risk of the women's reproduction disorder."
22917899
TP53 binds USP7, MDM2, and MDM4. 1 / 1
| 1
sparser
"Further insight into the mechanism by which ATM switches the interactions between HAUSP, Mdmx, Mdm2 and p53, to favor p53 activation may offer new tools for therapeutic intervention in the p53 pathway for cancer treatment."
16082221
TP53 binds USP10, USP4, USP7, and USP42. 1 / 1
| 1
sparser
"For example, the cell cycle-related protein p53 can bind USP4, USP7, USP10 and USP42, and then be deubiquitinated by these deubiquitinases [ xref , xref – xref ]."
30497519
TP53 binds USP7 and DAXX. 1 / 1
| 1
sparser
"MDM2 is a negative regulator of p53 that interacts with DAXX and HAUSP to form tertiary complex (MDM2-DAXX-HAUSP) [ xref ]."
30237857
Ubiquitin binds TP53, USP7, and MDM2. 1 / 1
| 1
sparser
"USP7 can bind Mdm2 or p53 via its N-terminal and C-terminal regions in a mutually exclusive manner, which consequently stabilizes the two proteins by removing ubiquitin."
28406480
Ubiquitin binds TP53, USP7, MDM2, and TraF. 1 / 1
| 1
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"The deubiquitinase USP7 binds Mdm2 or p53 via its N -terminal TRAF-domain or C-terminal region in a mutually exclusive manner to remove ubiquitins and stabilize the two proteins [ xref – xref ]."
26460617
AKT binds TP53 and USP7. 1 / 1
| 1
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"Although the essential role of USPs in protein degradation is well established, less is known about the function and regulation of specific family members: for example Usp7 has been associated with p53 and Akt turnover, Usp8 with receptor tyrosine endocytosis, Usp33 with the Von Hippel–Lindau disease (VHL) pathway and Usp19 is thought to have a role in muscle development [ xref ]."
25886905
TP53 binds USP7, DAXX, and EBNA1. 1 / 1
| 1
sparser
"We identify the ubiquitin-like (Ubl) domain 2 of USP7 as critical for the interaction and deubiquitination of p65 but dispensable for the interaction of USP7 with the USP7 substrates p53, DAXX, and EBNA1."
32587091
TP53 binds USP7, CDKN1A, and MDM2. 1 / 1
| 1
sparser
"In addition to the altered levels of HDM2, p53 and p21 associated with its use, P22077 treatment also caused reduced levels of DNA damage proteins DDB1, RBX1, DCAF7 and DCAF11, all of which are subunits of E3 ligases, indicating a previously unknown functional link between USP7 and enzymes involved in DNA repair [ xref ]."
23822887
Ubiquitinated TP53 binds USP7. 1 / 1
| 1
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"USP7 also interacts with the polyubiquitinated p53 and promotes p53 deubiquitination and stability."
20531303
GST binds TP53, USP7, MDM2, and NCL. 1 / 1
| 1
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"As expected, purified GST-p53 protein could bind Mdm2, nucleolin, and HAUSP ( xref , upper panel)."
26238070
TP53 binds USP7 and FOXO4. 1 / 1
| 1
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"The results of the interaction between the USP7 and FOXO4 in H1299 human lung carcinoma cells that lack p53 suggested that the interaction is p53 independent. xref , xref However, p53 and FOXOs have noticeable similarities such as the ability to stimulate cell-cycle arrest and cell death and they are both regulated by USP7. xref "
35005106
GST binds TP53 and USP7. 1 / 1
| 1
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"To confirm this phenomenon, we examined the direct interactions between p53 and Bat3 by mixing bacterially purified His-Bat3 and GST-p53 with Flag antibody-purified HAUSP or its deletion mutants followed by GST pull-down assay in vitro ."
31647545
N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal binds TP53 and USP7. 1 / 1
| 1
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"As shown in xref , ectopic expression of ABRO1 in HCT 116 p53 +/+ cells enhanced the interaction between USP7 and p53, whereas knockdown of ABRO1 weakened the interaction between USP7 and p53 in the presence of MG132 ( xref )."
25283148
TP53 binds USP10, USP7, and USP42. 1 / 1
| 1
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"In animal, USP7, USP10 and USP42 bind to and deubiquitylate the substrate p53 to counteract its degradation xref – xref ."
31745110
TP53 binds USP7, ABRAXAS2, and HCT116. 1 / 1
| 1
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"To confirm this, we examined the endogenous interaction between ABRO1 and USP7 in HCT116 p53 +/+ cells and found that this interaction could be enhanced by DNA damage ( xref )."
25283148
USP7 activates TP53.
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USP7 activates TP53. 10 / 100
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"Correspondingly, it is highly possible that the regulation of USP7 mediated p53 function by TSPY1 is an important molecular mechanism underlying its function in human spermatogenesis."
29748603
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"Chemical inhibition of USP7 and Wip1 impairs the viability of TP53 wild-type Ewing sarcoma cells."
30045945
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"The findings suggest an additional mechanism underlying the regulation of spermatogonial p53 function, indicating the significance of TSPY1 in germline homeostasis maintenance and the potential of TSPY1 in regulating human spermatogonial proliferation via the USP7 mediated p53 signaling pathway."
29748603
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"Overexpression of USP7 induces p53 activation, leading to growth suppression and apoptosis."
23151129
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"In addition to the ability of HAUSP to deubiquitinate PTEN and the relevance of the BCR-ABL/HAUSP/PTEN network in CML, HAUSP is also able to target p53."
27899971
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"Next we tested whether USP7 enhanced p53 function by examining the induction of p21 in H1299 cells after transfection of a p53 expressing plasmid alone or in combination with a plasmid expressing either WT USP7 or a USP7 mutant (XREF_FIG)."
20885946
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"In chronic lymphocytic leukemia (CLL), USP7 inhibition arrests cell growth and induces p53 independent apoptosis by restoring PTEN in the nucleus [XREF_BIBR]."
30897496
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"Still, the USP7-MDM2-p53 axis remains the paradigm of USP7 interactions in the nucleus, and new research continues to show how USP7 promotes p53 dependent apoptosis in disease."
30897496
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"As USP7 participates in regulating the P53-murine double minute-2 (MDM2) axis XREF_BIBR, abrogation of USP7 is considered to inactivate MDM2 and subsequently reactivate P53, leading to cell cycle arrest and apoptosis XREF_BIBR."
33777676
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"First, we tested whether USP7 could stimulate p53 function in a ubiquitin independent manner during conditions of cellular stress such as DNA damage."
20885946
Modified USP7 activates TP53. 2 / 2
| 2
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"The expression of USP7/HAUSP prevents p53 ubiquitination from Mdm2 as a p53-specific E3 ligase and increases the p53 protein stability [25]."
| PMC
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"Collectively, the data suggest that overexpression of USP7 up-regulates steady-levels of Poleta independent of p53 and suggested that Poleta, like p53, could be a substrate of USP7."
25435364
USP7 bound to WRAP53 activates TP53. 1 / 1
| 1
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"XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR In this study, we report a new molecular process in which WDR79 interacts with USP7 to modulate the stability of Mdm2 and p53, which promotes the proliferation of NSCLC cells."
28406480
USP7 deubiquitinates TP53.
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USP7 deubiquitinates TP53. 10 / 83
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"This result indicates that ABRO1 specifically facilitates USP7 mediated deubiquitination of p53."
25283148
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"USP7 deubiquitinates several tumour suppressors (p53, PTEN, FOXO and claspin) and E3 ligases (MDM2, Mule and viral proteins ICP0) and therefore regulates important signalling pathways that are involved in tumorigenesis XREF_BIBR XREF_BIBR."
25960197
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"The observation that HAUSP can directly interact with and deubiquitylate both p53 and MDM2 creates a conundrum : how can HAUSP stabilize p53 while at the same time being able to stabilize MDM2, which is primarily responsible for the destruction of p53?"
16402859
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"XREF_BIBR - XREF_BIBR USP7 deubiquitinates and stabilizes not only p53, but also Mdm2, the primary E3 ubiquitin ligase of p53 XREF_BIBR, XREF_BIBR Given that both p53 and Mdm2 are known regulators of the steady-level of Poleta, we speculated that changes in cellular USP7 levels may also modulate Poleta level."
25435364
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"Ubiquitin specific protease 7 (USP7) deubiquitinates p53 and Hdm2 and regulates their stability."
26786098
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"Via these interactions, MLF2 inhibits the binding of USP7 to p53 and antagonizes USP7-mediated deubiquitination of p53, thereby leading to p53 destabilization."
37438558
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"In particular, USP7 deubiquitinates p53 and WASH (part of the Wiskott–Aldrich Syndrome protein family), suggesting its role in disrupting tumor suppression pathways [23]."
32101552
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"USP7, also known as the hepes simplex virus associated ubiquitin specific protease (HAUSP), deubiquitinates both mdm2 and p53, and plays an important role in regulating the level and activity of p53."
20816748
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"In addition, USP7 may deubiquitinate p53 via Mdm2."
28406480
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"The first structure corresponds to USP7 (also known as HAUSP, Herpes associated USP), a DUB that preferentially deubiquitinates MDM2 (Murine Double Minute 2), the ubiquitin ligase for the tumor suppressor p53, as well as p53 itself XREF_BIBR."
19243136
Modified USP7 leads to the deubiquitination of TP53. 3 / 3
| 3
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"There also seems to be a delicate balance between these proteins, as a modest reduction of HAUSP levels prevents p53 deubiquitination but complete ablation of HAUSP causes robust p53 stabilization."
20404858
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"As shown in XREF_FIG, Mdm2 induced the ubiquitination of p53; however, p53 ubiquitination was significantly diminished by coexpression of USP10 or HAUSP."
20096447
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"The expression of USP7/HAUSP prevents p53 ubiquitination from Mdm2 as a p53-specific E3 ligase and increases the p53 protein stability [25]."
| PMC
USP7 deubiquitinates TP53 on lysine. 2 / 2
2 |
biopax:reactome
No evidence text available
biopax:pid
No evidence text available
17268548
USP7 deubiquitinates ubiquitinated TP53. 2 / 2
| 2
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"HAUSP specifically deubiquitinates the ubiquitinated p53 protein both in vitro and in vivo, and the expression of HAUSP was found to stabilize p53 in vivo and to promote p53 dependent cell growth arre[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12507430
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"Single- or double ubiquitinated p53 are deubiquitinated by a protein called HAUSP XREF_BIBR."
25504419
USP7 inhibits TP53.
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USP7 inhibits TP53. 10 / 56
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"Our results support a new role for vIRF1 through deregulation of the deubiquitinating enzyme USP7 to inhibit p53 mediated antiviral responses."
26786098
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"Notably, selective dual inhibitors of USP7 and USP47 have been synthesized and induced accumulation of p53 and apoptosis in human cancer cell lines [XREF_BIBR]."
25481052
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"As p53 is activated by genotoxic stress and has a key role in genotoxic stress induced cell death, we reasoned that pharmacological inhibition of USP7 activity would potentiate p53 activity and result in increased chemosensitivity."
24136231
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"The inhibition of USP7 with HBX 41,108, a DUB to Hdm2 has shown to induce p53 dependent apoptosis with an IC in sub-micro-molar concentrations."
24756373
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"Notably, USP7 overexpression has been demonstrated to stabilize MDM2 and destabilize p53, promoting p53 proteasomal degradation and diminishing p53 tumor suppressor downstream networks (Li et al., 2004)."
36186590
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"On the other hand, by ubiquitinating itself, MDM2 targets itself for destruction and promotes the p53 tumor suppressor pathway, a process that can be antagonized by the deubiquitinase herpesvirus associated ubiquitin specific protease (HAUSP)."
28196907
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"Typically , USP7 could stabilize MDM2 by deubiquitination and subsequently promotes the degradation of p53 , causing the inhibition of apoptosis in cancer cells ( Bhattacharya et al. 2018 ) ."
33165832
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"Inhibition of USP7 induces p53-independent tumor growth suppression in triple-negative breast cancers by destabilizing FOXM1."
37291217
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"Although several Daxx interacting proteins are involved in critical cellular pathways regarding P53 degradation, such as ubiquitin-specific-processing protease 7 (USP7) and mouse double minute 2 (Mdm2) [XREF_BIBR, XREF_BIBR], it is still unclear whether Daxx has any role in tumorigenicity of OSCC."
26205068
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"Furthermore, HAUSP inhibition elevates p53 stability and might be beneficial for therapeutic purposes."
27927749
Modified USP7 inhibits TP53. 2 / 2
| 2
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"Expression of USP7, the target protein of EBNA1 and ICP0, however, effectively promotes increased levels of p53 by antagonizing proteasomal degradation of p53 through deubiquitination (Li et al., 2004)."
17291557
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"Expression of USP7, the target protein of EBNA1 and ICP0, however, effectively promotes increased levels of p53 by antagonizing proteasomal degradation of p53 through deubiquitination."
17291557
USP7 bound to exosomal EBNA1 inhibits TP53. 1 / 1
| 1
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"In addition, exosomal EBNA1 can interact with USP7 to prevent the down-regulation of p53 XREF_BIBR, XREF_BIBR."
29581754
USP7 bound to TP53 inhibits TP53. 1 / 1
| 1
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"Furthermore, EBNA-1-USP7 interaction prevents the binding of USP7 to p53 and thereby diminishes p53 stabilization."
30807924
USP7 decreases the amount of TP53.
| 25
USP7 decreases the amount of TP53. 10 / 26
| 24
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"We have previously reported that the ubiquitin-specific peptidase 7 (USP7) may be a novel target for senolysis as inhibition of USP7 can selectively induce apoptosis of SnCs by increasing the level of p53 (He et al., 2020a) ."
35252191
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"On the other side, the ubiquitinated TP53 could be reversed and stabilized by USP7 and USP10, to keep the amount of TP53 in check."
31695837
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"USP7 (ubiquitin specific protease 7), which deubiquitinates HDM2, can lead to increased levels of HDM2 and decreased levels of p53."
22649774
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"Moreover, these changes correlated with the levels of p53, indicating that USP7 inhibition can partially restore the expression of p53 and its downstream pro‐apoptotic proteins in SnCs."
32064756
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"However, p53 levels in P5091‐treated WI‐38 SnCs remained slightly lower than the basal level of p53 in untreated non‐SnCs, suggesting that USP7 inhibition only partially restored the basal levels of p53 in WI‐38 SnCs and did not dramatically increase p53 expression as seen in WI‐38 cells after exposure to IR (Figure 1a, c)."
32064756
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"Although a partial reduction in HAUSP can increase p53 levels, drastic reduction can have the opposite effect [17]."
15865937
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"However depletion of HAUSP in cells does not decrease p53 levels as predicted, but rather increases p53 levels, apparently due to HAUSP 's ability to bind and deubiquitinate Mdm2."
20096447
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"There is precedent for this possibility as, under some circumstances, USP7 can negatively regulate p53 levels by stabilizing the Mdm2 E3 ligase XREF_BIBR, XREF_BIBR."
21305000
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"Inhibition of USP7 is expected to increase p53 levels, leading to anti-tumour activity [79], [80]."
26852935
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"These findings are in agreement with our suggestion that USP7 inhibition upregulates p53 expression at least in part via promoting MDM2 proteasome degradation."
32064756
USP7 decreases the amount of ubiquitinated TP53. 1 / 1
| 1
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"Conversely, FAM188B downregulation increased unbound USP7 and this free USP7 may decrease the level of ubiquitinated p53."
29795372
USP7 increases the amount of TP53.
| 17
USP7 increases the amount of TP53. 10 / 13
| 13
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"Besides, protein expression of p53 and p21 was dramatically downregulated by either GMPS or USP7 knockdown in MCF-7 and DU4475 cells (Fig. 4f, g)."
33742136
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"While USP7 can directly regulate p53 levels, these levels can also be regulated by the DUB USP2a."
18687060
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"Initially, USP7 was believed to primarily deubiquitinate p53, increasing the level of p53 [17] ."
18793145
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"By simulating TSPY1 function in Tspy1 deficient spermatogonia derived from mouse testes, we found that TSPY1 could promote spermatogonial proliferation by decreasing the Usp7 modulated p53 level."
29748603
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"Both, the DUB HAUSP (herpesvirus associated ubiquitin specific protease) and USP10 (ubiquitin specific protease 10), targeting poly-ubiquitinated p53, have been shown to restore p53 levels even when Hdm2 is overexpressed."
24756373
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"However, knockout of USP7 in cells does not decrease p53 levels as predicted, but rather stabilizes p53 [XREF_BIBR]."
26460617
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"As shown in XREF_FIG, knockdown of USP7 reduced the levels of Mdm2 and p53, a result consistent with that of a previous study."
28406480
reach
"However depletion of HAUSP in cells does not decrease p53 levels as predicted, but rather increases p53 levels, apparently due to HAUSP 's ability to bind and deubiquitinate Mdm2."
20096447
reach
"Silencing of USP7 decreased the p53 level and increased ubiquitinated-p53."
29795372
reach
"By regulating p53 levels through their deubiquitinating activities, USP7 and USP2a may contribute to cancer pathogenesis and therapeutic strategies that target these p53 specific DUBs may become important as cancer treatments [XREF_BIBR]."
18687060
Modified USP7 increases the amount of TP53. 4 / 4
| 4
reach
"In addition, USP7 overexpression significantly increased endogenous Mdm2 and p53 levels, but had no effect on STIP levels (XREF_SUPPLEMENTARY)."
26460617
reach
"Expression of USP7, the target protein of EBNA1 and ICP0, however, effectively promotes increased levels of p53 by antagonizing proteasomal degradation of p53 through deubiquitination (Li et al., 2004)."
17291557
reach
"As shown by the Gu group, partial reduction of USP7 levels in several human cell lines promotes decreased levels of both MDM2 and p53, yet total abolition of USP7 stabilizes p53 levels by decreasing MDM2 [117, 118]."
24239534
reach
"Expression of USP7, the target protein of EBNA1 and ICP0, however, effectively promotes increased levels of p53 by antagonizing proteasomal degradation of p53 through deubiquitination."
17291557
USP7 ubiquitinates TP53.
| 5 2
USP7 ubiquitinates TP53. 6 / 7
| 4 2
reach
"Moderate down regulation of HAUSP reduces the deubiquitination of p53, leading to p53 destabilization and therefore favors cell proliferation [XREF_BIBR]."
20932800
reach
"Different strategies are being employed to achieve this, including Ube1 inhibition to prevent Ub activation, HDM2 inhibition to block p53 polyubiquitination, and USP7 and HAUSP inhibition to promote HDM2 degradation."
23822887
sparser
"Fluorimetric quantification revealed that the USP7-GMPS complex ubiquitylates p53 at a rate that is more than 20 times faster than USP7 alone (compare USP7 alone at 20′ with USP7-GMPS at 1′)."
15749019
sparser
"On one hand, p53 binds to TRAF domain and C-terminal (amino acids 880–1050) of USP7, and then USP7 ubiquitinates p53 directly and prevents it from degradation."
31114498
reach
"Fluorimetric quantification revealed that the USP7 and GMPS complex ubiquitylates p53 at a rate that is more than 20 times faster than USP7 alone (compare USP7 alone at 20 ' with USP7-GMPS at 1 ')."
15749019
reach
"We further determined whether ABRO1 facilitates p53 deubiquitination in a USP7 dependent manner; as shown in XREF_FIG, knockdown of USP7 impaired ABRO1 mediated p53 stability and deubiquitination."
25283148
Modified USP7 leads to the ubiquitination of TP53. 1 / 1
| 1
reach
"The expression of USP7/HAUSP prevents p53 ubiquitination from Mdm2 as a p53-specific E3 ligase and increases the p53 protein stability [25]."
| PMC
USP7 acetylates TP53.
| 1
USP7 acetylates TP53 on K120. 1 / 1
| 1
sparser
"USP7 also increases Tip60-dependent p53-K120 acetylation, which is required for its induction of the pro-apoptotic gene, PUMA [ xref ]."
25545885
TP53 affects USP7
29 2 | 1 120 181 1
TP53 binds USP7.
29 2 | 1 92 181
TP53 binds USP7. 10 / 235
29 2 | 1 77 105
reach
"Surprisingly, however, we have found that direct interaction between HAUSP and p53 is not absolutely required for it to antagonize efficiently Mdm2 mediated ubiquitination of p53 and that HAUSP is capable of enzymatically functioning in trans on p53 by using Mdm2 as a bridge."
17525743
sparser
"The results of the present study were consistent with these previous findings, demonstrating that the deletion and inhibition of HAUSP was associated with p53 upregulation ( xref )."
27899971
reach
"USP7 interacts and regulates p53 as well as MDM2 stability by promoting their deubiquitination (Li et al., 2002; Ma et al., 2010)."
30067985
sparser
"The N-terminal region of Bat3 containing an ubiquitin-linked domain was found to have capacity to form a stable complex with both HAUSP ( xref , lane 3) and p53 ( xref , lane 3), but other deletions, which are lacking the N-terminus, completely abolish the ability to form complexes ( xref , lanes 4 and 5; xref , lanes 5 and 6)."
31647545
sparser
"Intriguingly, Becker et al. described that the hyperubiquitylation of p53 contributes to its aberrant cytoplasmic retention in neuroblastoma in association with the impaired interaction between p53 and HAUSP which catalyzes the deubiquitylation of p53 [ xref ]."
21188172
sparser
"Moreover, USP7 can interact with p53 and promote its expression through mediating its deubiquitination."
35538032
sparser
"Interestingly, STIP knock down seem to have a more profound impact on the interaction between USP7 and Mdm2 than the interaction between USP7 and p53."
26460617
reach
"The interactions between HAUSP and p53 or MDM2 are likely to be more complex in vivo, not only due to the presence of multiple binding sites in MDM2 but also because of the oligomeric nature of p53 and possibly HAUSP."
16402859
reach
"Consistent with this, our results reveal that STIP knock down have a more profound impact on the interaction between USP7 and Mdm2 than the interaction between USP7 and p53."
26460617
reach
"As efforts to obtain crystals of the p53 and HAUSP complex were not successful, they generated protein chimeras -- made of half p53 peptides and half HAUSP TRAF like domain -- to determine the complex 's structure and mechanism of binding."
20076526
TP53 binds USP7 and MDM2. 10 / 51
| 13 38
sparser
"In addition, USP7 can interact indirectly with p53, using Mdm2 as a bridge, resulting in the formation of USP7-Mdm2-p53 complexes."
26460617
reach
"The HAUSP, p53, and Mdm2 complex and p53-nucleolin interplay in DNA damage have been identified XREF_BIBR XREF_BIBR XREF_BIBR; however, the molecular relation between these two findings was poorly understood."
26238070
sparser
"Taken together, the activation of USP7-MDM2-p53 interaction can promote the occurrence and development of tumors."
31114498
reach
"P53, as a tumour-suppressor, exerts an essential role in signalling associated with the control of cell survival and aberrant p53 signalling results in unchecked cell growth and the initiation of cancer.USP7 interacts with a range of protein targets in the p53 pathway (p53, MDMX, MDM2)39 and MDM2 plays a key role in p53 stabilization by increasing the ubiquitination process.39 USP7 deubiquitinates MDM2 and its functional regulator MdmX and stabilizes p53.39 USP7 apparently has 2 independent p53 regulatory functions, stabilizing p53 through deubiquitylation and regulating the sequence-specific DNA binding of p53.40 Within the USP7, MDM2 and p53 complex, p53 and the MDM2 have been shown to bind USP7 through the TRAF domain.39 Under normal conditions, USP7 prefers MDM2 as a substrate rather than p53 and MDM2 has a higher binding affinity for USP7 compared to p53.41 Reduction of USP7 expression levels leads to p53 stabilisation, and destabilization of MDM2."
35005106
reach
"The authors find that genotoxic stress induces a shift in complex formation from a p53, MDM2, and USP7 complex to p53/GMPS/USP7."
24507714
sparser
"Binding of HAUSP to either p53 or MDM2 leads to their de-ubiquitination, and binding to MDM2 leads to p53 destabilization due to increased MDM2 stability [ xref ]."
19538162
sparser
"USP7 can bind directly to Mdm2 or p53 in a mutually exclusive manner."
26460617
sparser
"Although USP7 can interact with both Mdm2 and p53 depending on the cellular context, USP7 preferentially forms a stable USP7-Mdm2 complex even in the presence of excess p53 [ xref ], indicating that USP7 predominantly functions to stabilize Mdm2."
33207738
reach
"However, the linkage between the DNA damage response machinery and the HAUSP, p53, and Mdm2 complex in DNA damaged cells still remain unresolved."
26238070
reach
"Taken together, this study reveals a new component of the HAUSP, p53, and Mdm2 complex that governs dynamic cellular responses to DNA damage."
26238070
TP53 binds USP10 and USP7. 3 / 3
| 3
sparser
"For example, USP7 and USP10 bind to and deubiquitylate their substrate p53 to mediate its role for suppression of cell propagation upon cellular stresses by counteracting its degradation xref – xref ."
31745110
sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced under DMOG treatment ( xref A)."
30067985
sparser
"Using a quantitative mass spectrometry approach and focusing on ubiquitin ligases and proteases, we were able to determine that the interaction between p53 and two DUBs, USP7 and USP10, was reduced un[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30067985
TP53 binds USP7 and BAG6. 3 / 3
| 3
sparser
"Notably, we revealed that a p53–HAUSP–Bat3 trimeric protein complex could exist in vivo with HAUSP serving as a binding mediator for enhanced interaction between p53 and Bat3, which antagonizes the interaction of p53 with its proteasome receptor S5a and promotes the accumulation of p53 in the nucleus."
31647545
sparser
"These results suggest that the formation of p53–HAUSP–Bat3 complex may prevent p53 from being recognized and binding to its proteasome receptor S5a, subsequently leading to the accumulation and stabilization of p53 in nucleus ( xref ) though we still do not know whether there is conformational change in Bat3 and p53 at present."
31647545
sparser
"Immunoprecipitation of protein extracts with anti-p53 antibody and subsequent immunoblot analysis revealed a trimeric protein interaction between p53, Bat3 and HAUSP as both HA-Bat3 and Flag-HAUSP were detected in the p53-immunoprecipitated complex ( xref )."
31647545
TP53 binds USP7 and EBNA1. 3 / 3
| 3
sparser
"Crystal structure analysis showed that USP7 binds to its substrate p53 and its inhibitory interactor Epstein–Barr nuclear antigen 1 (EBNA1) protein through the same pocket but the former binding partner p53 exhibit weaker contacts with USP7 [ xref , xref ]."
28031783
sparser
"NMR studies of USP7 bound by EBNA1 and p53 indicated that p53 and EBNA bind the same pocket but p53 but makes less extensive contacts with USP7."
19489724
sparser
"The results of these experiments demonstrated that the deletion of Ubl2 did not inhibit the interaction of USP7 with p53 or EBNA1 ( xref , A and B )."
32587091
TP53 binds USP7 and GMPS. 2 / 2
| 2
sparser
"In the context of X-ray radiation, GMPS bound both USP7 and TP53 (Fig. xref b), and TP53 protein levels were elevated upon GMPS overexpression (Additional file xref : Figure S1e), suggesting that GMPS promotes TP53 protein stability."
32005234
sparser
"Considering that the interaction among GMPS, USP7 with p53 is required for the deubiquitination and stabilization of p53 [ xref , xref ]. we next determined the interaction of GMPS and USP7 with p53 in the p53wt breast cancer cell lines MCF-7 and DU4475 by co-IP assays."
33742136
TP53 binds USP7, WRAP53, and MDM2. 2 / 2
| 2
sparser
"In this study, we report a new molecular process in which WDR79 interacts with USP7 to modulate the stability of Mdm2 and p53, which promotes the proliferation of NSCLC cells."
28406480
sparser
"Taken together, our findings reveal a novel role of WDR79 in the proliferation of NSCLC cells and could pinpoint a new mechanism by which WDR79 and USP7 functionally interact to modulate the Mdm2-p53 pathway."
28406480
TRAF binds TP53 and USP7. 2 / 2
| 2
sparser
"As shown in xref , p53 has 393 amino acid residues that can be subdivided into five domains: the N-terminal transactivation domains (TAD) that are responsible for its binding to the p53-binding sites on MDM2 and MDMX, a proline-rich region (PP) that contains five PxxP motifs and is essential for inducing apoptosis, a DNA-binding domain (DBD), a tetramerization domain (TET), and a C-terminal domain (CTD) that is critical for the binding of p53 to the TRAF domain of USP7 ( xref ; xref )."
32300595
sparser
"On one hand, p53 binds to TRAF domain and C-terminal (amino acids 880–1050) of USP7, and then USP7 ubiquitinates p53 directly and prevents it from degradation."
31114498
TP53 binds USP7, DAXX, and MDM2. 2 / 2
| 1 1
reach
"The most significant binding partners identified to interact with the C1 domain are the TNF-R1 and TRAIL-R1 -- Modulator of Apoptosis-1 (MOAP-1) complexes and the MDM2, DAXX, HAUSP, and p53 complex [XREF_BIBR, XREF_BIBR] (XREF_FIG)."
22577550
sparser
"Although the molecular mechanism for the interactions of DAXX, HAUSP, Mdm2, and p53 underlying this regulatory pathway are largely unknown, an important clue is provided by our observation that, at le[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21134643
TP53 binds USP7, ELP2, and MDM2. 2 / 2
| 2
sparser
"Considering that the effect of STIP on Mdm2 or p53 was independent of each other, we hypothesize that STIP may associate with the USP7-Mdm2 or USP7-p53 complexes."
26460617
sparser
"These data indicated that STIP may simultaneously bind to USP7 and either Mdm2 or p53, mediating the assembly of ternary STIP-USP7-Mdm2 and STIP-USP7-p53 complexes, respectively."
26460617
TP53 binds USP7, MDM2, and NCL. 2 / 2
| 2
sparser
"We next verified that HAUSP and nucleolin could form a complex with p53 and Mdm2 by examining the interaction between HAUSP and nucleolin in p53- and Mdm2-depleted cells."
26238070
sparser
"Moreover, p53 also could not interact with HAUSP and nucleolin in Mdm2-depleted cells ( xref )."
26238070
TP53 binds USP7 and ABRAXAS2. 2 / 2
| 2
sparser
"Based on the results showing association of ABRO1, p53 and USP7, we presumed that ABRO1 might promote interaction between USP7 and p53, and regulate USP7-dependent deubiquitination of p53."
25283148
sparser
"ABRO1 forms a complex with USP7 and p53."
25283148
TP53 binds USP7 and NCL. 1 / 1
| 1
sparser
"We hypothesized that Mdm2 acts as a mediator between HAUSP and nucleolin in the normal state, and that p53 may be associated with the HAUSP and nucleolin interaction in the DNA damaged condition."
26238070
TP53 binds USP7 and MDM4. 1 / 1
| 1
sparser
"It has also been reported that some SNPs of p53, Mdm2(Murine double minute 2), MdmX and Hausp (Herpes virus-associated ubiquitin-specific protease) in p53 pathway are associated with the risk of the women's reproduction disorder."
22917899
TP53 binds USP7, MDM2, and MDM4. 1 / 1
| 1
sparser
"Further insight into the mechanism by which ATM switches the interactions between HAUSP, Mdmx, Mdm2 and p53, to favor p53 activation may offer new tools for therapeutic intervention in the p53 pathway for cancer treatment."
16082221
TP53 binds USP10, USP4, USP7, and USP42. 1 / 1
| 1
sparser
"For example, the cell cycle-related protein p53 can bind USP4, USP7, USP10 and USP42, and then be deubiquitinated by these deubiquitinases [ xref , xref – xref ]."
30497519
TP53 binds USP7 and DAXX. 1 / 1
| 1
sparser
"MDM2 is a negative regulator of p53 that interacts with DAXX and HAUSP to form tertiary complex (MDM2-DAXX-HAUSP) [ xref ]."
30237857
Ubiquitin binds TP53, USP7, and MDM2. 1 / 1
| 1
sparser
"USP7 can bind Mdm2 or p53 via its N-terminal and C-terminal regions in a mutually exclusive manner, which consequently stabilizes the two proteins by removing ubiquitin."
28406480
Ubiquitin binds TP53, USP7, MDM2, and TraF. 1 / 1
| 1
sparser
"The deubiquitinase USP7 binds Mdm2 or p53 via its N -terminal TRAF-domain or C-terminal region in a mutually exclusive manner to remove ubiquitins and stabilize the two proteins [ xref – xref ]."
26460617
AKT binds TP53 and USP7. 1 / 1
| 1
sparser
"Although the essential role of USPs in protein degradation is well established, less is known about the function and regulation of specific family members: for example Usp7 has been associated with p53 and Akt turnover, Usp8 with receptor tyrosine endocytosis, Usp33 with the Von Hippel–Lindau disease (VHL) pathway and Usp19 is thought to have a role in muscle development [ xref ]."
25886905
TP53 binds USP7, DAXX, and EBNA1. 1 / 1
| 1
sparser
"We identify the ubiquitin-like (Ubl) domain 2 of USP7 as critical for the interaction and deubiquitination of p65 but dispensable for the interaction of USP7 with the USP7 substrates p53, DAXX, and EBNA1."
32587091
TP53 binds USP7, CDKN1A, and MDM2. 1 / 1
| 1
sparser
"In addition to the altered levels of HDM2, p53 and p21 associated with its use, P22077 treatment also caused reduced levels of DNA damage proteins DDB1, RBX1, DCAF7 and DCAF11, all of which are subunits of E3 ligases, indicating a previously unknown functional link between USP7 and enzymes involved in DNA repair [ xref ]."
23822887
Ubiquitinated TP53 binds USP7. 1 / 1
| 1
reach
"USP7 also interacts with the polyubiquitinated p53 and promotes p53 deubiquitination and stability."
20531303
GST binds TP53, USP7, MDM2, and NCL. 1 / 1
| 1
sparser
"As expected, purified GST-p53 protein could bind Mdm2, nucleolin, and HAUSP ( xref , upper panel)."
26238070
TP53 binds USP7 and FOXO4. 1 / 1
| 1
sparser
"The results of the interaction between the USP7 and FOXO4 in H1299 human lung carcinoma cells that lack p53 suggested that the interaction is p53 independent. xref , xref However, p53 and FOXOs have noticeable similarities such as the ability to stimulate cell-cycle arrest and cell death and they are both regulated by USP7. xref "
35005106
GST binds TP53 and USP7. 1 / 1
| 1
sparser
"To confirm this phenomenon, we examined the direct interactions between p53 and Bat3 by mixing bacterially purified His-Bat3 and GST-p53 with Flag antibody-purified HAUSP or its deletion mutants followed by GST pull-down assay in vitro ."
31647545
N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal binds TP53 and USP7. 1 / 1
| 1
sparser
"As shown in xref , ectopic expression of ABRO1 in HCT 116 p53 +/+ cells enhanced the interaction between USP7 and p53, whereas knockdown of ABRO1 weakened the interaction between USP7 and p53 in the presence of MG132 ( xref )."
25283148
TP53 binds USP10, USP7, and USP42. 1 / 1
| 1
sparser
"In animal, USP7, USP10 and USP42 bind to and deubiquitylate the substrate p53 to counteract its degradation xref – xref ."
31745110
TP53 binds USP7, ABRAXAS2, and HCT116. 1 / 1
| 1
sparser
"To confirm this, we examined the endogenous interaction between ABRO1 and USP7 in HCT116 p53 +/+ cells and found that this interaction could be enhanced by DNA damage ( xref )."
25283148
TP53 activates USP7.
| 17
TP53 activates USP7. 10 / 15
| 15
reach
"To determine whether there was increased apoptosis due to p53 activation in hausp knockout embryos, a TUNEL (TdT mediated dUTP Nick-End Labeling) assay was performed on wild-type and hausp knockout embryos of both days E6.5 (XREF_FIG) and E7.5 (XREF_FIG)."
19946331
reach
"Interestingly, the level of Mdm4 showed slight increase in hausp knockout MEFs (XREF_FIG, lane 6 versus lane 5), suggesting p53 activation in hausp knockout MEFs was mainly because of downregulation of Mdm2."
21350561
reach
"The results showed that knockout of p53 did not rescue the lethality of the hausp knockout."
19946331
reach
"10, 13, 14 The identification of these substrates indicates that USP7 controls additional vital cellular functions beyond those mediated by p53 stability."
33650720
reach
"We therefore observed p53 independent induction of p21 protein that was followed by cell-cycle arrest in G1 phase, as previously described."
22361354
reach
"Once again, the current study demonstrated the essential roles of p53 independent functions of HAUSP, indicated by the inability to rescue the neonatal lethality of hausp FL/FL; nes-cre mice by concomitant deletion of p53."
21350561
reach
"Hdm2 is preferentially targeted by USP7 over p53 under normal unstressed conditions; however, USP7 targets p53 in response to DNA damage."
25605410
reach
"Hausp knockout was partially rescued by p53 knockout."
19946331
reach
"Although deletion of p53 did not completely rescue the embryonic lethality of the hausp knockout, embryonic development was extended in both hausp and p53 double knockout embryos."
19946331
reach
"Owing to the observation of p53 activation in hausp knockout embryos, we attempted to rescue the lethality of hausp knockout mice by generating hausp and p53 double knockout mice."
19946331
TP53 bound to SGTA activates USP7. 1 / 1
| 1
reach
"In a well studied example, the N-terminal domain of the human UBP called herpesvirus associated ubiquitin specific protease ([HAUSP] USP7) binds the p53 tumor suppressor, allowing HAUSP to cleave polyubiquitin-p53 conjugates and, thereby, limit p53 degradation."
17145966
TP53 bound to USP7 activates USP7. 1 / 1
| 1
reach
"Conflicting studies show that binding of p53 to USP7 either promotes the deubiquitination and subsequent stabilization of p53 [XREF_BIBR, XREF_BIBR] or that disruption of USP7 stabilizes p53 [XREF_BIBR, XREF_BIBR]."
20210752
TP53 inhibits USP7.
| 8
TP53 inhibits USP7. 7 / 8
| 7
reach
"However, whether any of these mechanisms contribute to USP7 inhibition‐induced SnC apoptosis has yet to be determined.The results from our studies also suggest that p53 may mediate USP7 inhibition‐induced SnC apoptosis via both transcriptional and post‐transcriptional mechanisms."
32064756
reach
"These results suggested that although deletion of p53 failed to rescue the neonatal lethality of hausp FL/FL; nes-cre mice, survival of neural cells and brain development were largely restored in the absence of p53."
21350561
reach
"To determine whether p53 mediates USP7 inhibition‐induced SnC apoptosis by upregulating pro‐apoptotic genes, we compared BBC3, PMAIP1, and FAS mRNA levels in non‐SnCs and IR‐induced SnCs with or without P5091 treatment."
32064756
reach
"XREF_BIBR, XREF_BIBR The p53 staining was weak but was detectable in some of the cells in hausp FL/FL; nes-cre cortex at day E12.5 (data not shown), indicating the onset of the accumulation of p53 following depletion of HAUSP protein."
21350561
reach
"P53 levels were increased by compound 4 in all three cell lines, confirming its inhibition of USP7."
30367141
reach
"Concomitant deletion of p53 partially rescued the developmental defects in hausp nes-cre conditional knockout mice, providing direct evidences for p53 dependent functions of HAUSP."
21350561
reach
"As shown in XREF_FIG, both p53 and Mdm2 had decreased half-lives in hausp heterozygote MEF cells (lanes 5-8) compared with that in wild-type MEF cells (lanes 1-4)."
19946331
TP53 bound to TSPYL5 inhibits USP7. 1 / 1
| 1
reach
"In HEK293 cells, we observed that TSPYL5 could competitively bind to the N-terminal domain of deubiquitylase USP7 in conjunction with p53 and reduce the protective effects of USP7 on p53, resulting in ubiquitin mediated degradation of p53, and that the co-transfection of TSPY1 and TSPYL5 enhanced this effect."
29748603
TP53 deubiquitinates USP7.
| 2
TP53 deubiquitinates USP7. 2 / 2
| 2
reach
"It stabilizes p53 and induces p53-dependent apoptosis in cancer cells through inhibition of the p53-deubiquitinating enzyme USP7 [92]."
| PMC
reach
"Knockdown of the p53 deubiquitinating enzyme USP7 and HAUSP also reverses the supervillin phenotype, blocking the increase in p53 levels seen after supervillin knockdown and accentuating the decrease in p53 levels triggered by supervillin overexpression."
23382381
TP53 phosphorylates USP7.
| 1
TP53 phosphorylates USP7. 1 / 1
| 1
rlimsp
"However, only after coexpression of p53 with Mdm2 and USP7S was the protein level of p53 reduced significantly further (Figure 2H, lane 4), confirming that phosphorylated USP7S efficiently controls the cellular level of p53."
22361354
TP53 increases the amount of USP7.
| 1
TP53 increases the amount of USP7. 1 / 1
| 1
reach
"Their results indicated that p53 loss did not completely rescue the loss of HAUSP, leaving open a role for PTEN in HAUSP-cKO mice."
24860420
EBNA1 affects MCMBP, MDM2, MDM4, TP53, UBE2E1, USP7, vIRF-1, and vIRF-4
| 1
TP53 binds UBE2E1, USP7, MCMBP, MDM2, MDM4, vIRF-1, EBNA1, and vIRF-4. 1 / 1
| 1
sparser
"USP7 can interact with p53, HDM2, HDMX, MCM-BP, UbE2E1, vIRF1, vIRF4, and EBNA1 through the TRAF-like domain [ xref , xref , xref , xref , xref , xref , xref ]."
33498168
EBNA1 affects DAXX
| 1
TP53 binds USP7, DAXX, and EBNA1. 1 / 1
| 1
sparser
"We identify the ubiquitin-like (Ubl) domain 2 of USP7 as critical for the interaction and deubiquitination of p65 but dispensable for the interaction of USP7 with the USP7 substrates p53, DAXX, and EBNA1."
32587091
DAXX affects TP53, and USP7
| 1
TP53 binds USP7 and DAXX. 1 / 1
| 1
sparser
"MDM2 is a negative regulator of p53 that interacts with DAXX and HAUSP to form tertiary complex (MDM2-DAXX-HAUSP) [ xref ]."
30237857
DAXX affects EBNA1, TP53, and USP7
| 1
TP53 binds USP7, DAXX, and EBNA1. 1 / 1
| 1
sparser
"We identify the ubiquitin-like (Ubl) domain 2 of USP7 as critical for the interaction and deubiquitination of p65 but dispensable for the interaction of USP7 with the USP7 substrates p53, DAXX, and EBNA1."
32587091
CDKN1A affects USP7
| 1
TP53 binds USP7, CDKN1A, and MDM2. 1 / 1
| 1
sparser
"In addition to the altered levels of HDM2, p53 and p21 associated with its use, P22077 treatment also caused reduced levels of DNA damage proteins DDB1, RBX1, DCAF7 and DCAF11, all of which are subunits of E3 ligases, indicating a previously unknown functional link between USP7 and enzymes involved in DNA repair [ xref ]."
23822887
ATRX affects MDM2, RASSF5, TP53, TP63, TP73, and USP7
| 1
TP53 binds TP73, USP7, TP63, RASSF5, MDM2, and ATRX. 1 / 1
| 1
sparser
"The DAXX helical bundle (DHB) domain has been reported to interact with ATRX, RASSF1C, MDM2, HAUSP, P53, P63, and P73 (Escobar-Cabrera et al., xref ; Gostissa et al., xref ; Tang et al., xref ; Tang et al., xref )."
28875424
AKT affects USP7
| 1
AKT binds TP53 and USP7. 1 / 1
| 1
sparser
"Although the essential role of USPs in protein degradation is well established, less is known about the function and regulation of specific family members: for example Usp7 has been associated with p53 and Akt turnover, Usp8 with receptor tyrosine endocytosis, Usp33 with the Von Hippel–Lindau disease (VHL) pathway and Usp19 is thought to have a role in muscle development [ xref ]."
25886905
ABRAXAS2 affects HCT116, TP53, and USP7
| 1
TP53 binds USP7, ABRAXAS2, and HCT116. 1 / 1
| 1
sparser
"To confirm this, we examined the endogenous interaction between ABRO1 and USP7 in HCT116 p53 +/+ cells and found that this interaction could be enhanced by DNA damage ( xref )."
25283148