IndraLab

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USP7 decreases the amount of TP53. 46 / 48
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"The inhibition of USP7 stimulated transcription of p53-associated genes related to cell-cycle arrest and apoptosis."
34089649
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"Saridakis et al. have demonstrated that the USP7, a key regulator of p53, is strongly targeted by EBNA1 and consequently can lower p53 levels."
28729047
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"Similarly, in our study, we confirmed that knockdown of USP7 increases the p53 expression."
35948545
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"Conversely, USP7 overexpression is expected to decrease p53 expression, but does not affect p53 protein expression."
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"This suggests USP7 may negatively regulate p53 levels through MDM2."
25519684
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"Initially identified as a p53 DUB, it was later shown that, conversely, USP7 impairs p53 expression and function by regulating Mdm2 ubiquitylation [XREF_BIBR], and in so doing exerts pro oncogenic functions."
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"To further determine whether MLF2 suppresses p53 expression via USP7, we ectopically expressed p53 together with USP7 or both USP7 and MLF2 in Mdm2 p53 mouse embryonic fibroblast (MEF) cells."
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"USP7 inhibition in NB cells activated the p53 pathway via USP7 and MDM2 degradation, leading to reduced p53 ubiquitination and increased p53 expression in all sensitive NB cells."
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"On the other side, the ubiquitinated TP53 could be reversed and stabilized by USP7 and USP10, to keep the amount of TP53 in check."
31695837
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"Absence of HAUSP promoted the ubiquitinated form and thereby proteosomal degradation of MDM2, a negative regulator of p53 levels (14)."
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"Moreover, Co-IP assay revealed that inhibition of USP7 could promote the ubiquitination level of p53 ( Figure 3F)."
35538032
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"FT671, a noncovalent inhibitor of USP7, can increase the degradation of the Ub ligase MDM2 and the expression of p53 to exert tumour-suppressing effects in vivo (190)."
36969062
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"Notably, the inhibition of HAUSP with small molecule inhibitors promoted the upregulation of p53 protein levels."
27899971
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"Although single treatment with low dose of either agent was unable to induce apoptosis, remarkably, the combination of HAUSP and Mdm2 inhibition can strikingly activate p53 levels and induce apoptosis.Since acetylation is critical for p53 activity, targeting the deacetylating enzymes may be a good choice to activate p53."
31282934
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"Compound 1, a selective inhibitor of USP7, elevates p53 levels and apoptosis in cancer cells and also exhibits tumor suppressive activity in xenograft multiple myeloma and B-cell leukemia models [213]."
34814083
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"Subsequently, it was shown that while partial knockdown of USP7 decreased p53 levels, complete ablation of USP7 led to an increase in p53 levels."
20713061
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"We have previously reported that the ubiquitin-specific peptidase 7 (USP7) may be a novel target for senolysis as inhibition of USP7 can selectively induce apoptosis of SnCs by increasing the level of p53 (He et al., 2020a) ."
35252191
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"These findings are in agreement with our suggestion that USP7 inhibition upregulates p53 expression at least in part via promoting MDM2 proteasome degradation."
32064756
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"Although a partial reduction in HAUSP can increase p53 levels, drastic reduction can have the opposite effect [17]."
15865937
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"The mechanism by which USP7 inhibition increases p53 expression is likely attributable in part to the reduction of MDM2 expression as USP7 stabilizes MDM2 via deubiquitinating MDM2 (Li et al., 2004)."
32064756
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"USP7 inhibition in NB cells activated the p53 pathway via USP7 and MDM2 degradation, leading to reduced p53 ubiquitination and increased p53 expression in all sensitive treated NB cells."
37762082
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"USP7 silencing has been shown to increase steady-state p53 level by promoting MDM2 degradation."
34552711
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"HCT116 USP7 -/- cells grow extremely slowly because USP7 knockout leads to constitutively high level of p53 and p21."
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"However, p53 levels in P5091‐treated WI‐38 SnCs remained slightly lower than the basal level of p53 in untreated non‐SnCs, suggesting that USP7 inhibition only partially restored the basal levels of p53 in WI‐38 SnCs and did not dramatically increase p53 expression as seen in WI‐38 cells after exposure to IR (Figure 1a, c)."
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"Moreover, these changes correlated with the levels of p53, indicating that USP7 inhibition can partially restore the expression of p53 and its downstream pro‐apoptotic proteins in SnCs."
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"In addition to inhibiting the degradation of c-Maf in proteosomes, USP7 can stabilize MDM2, a ubiquitin ligase of p53, reducing p53 expression and promoting cancer cell survival [148]."
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"EBNA1 interacts with ubiquitin-specific protease 7 (USP7) to decrease P53 levels [75]."
36430864
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"Knockdown of USP7 or USP47 increased the protein levels of p53 and the mRNA levels of CDKN1A, a p53 target gene encoding p21 protein, without affecting p53 gene expression (Figs."
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"There is precedent for this possibility as, under some circumstances, USP7 can negatively regulate p53 levels by stabilizing the Mdm2 E3 ligase XREF_BIBR, XREF_BIBR."
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"USP7 (ubiquitin specific protease 7), which deubiquitinates HDM2, can lead to increased levels of HDM2 and decreased levels of p53."
22649774
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"USP7/HAUSP can elongate p53; however, depletion of USP7/HAUSP also induces upregulation of p53 protein expression [27]."
| PMC
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"The stabilized USP7/HAUSP can enhance Mdm2 and decrease p53 protein expression."
| PMC
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"USP7 strongly stabilizes p53 by deubiquitinating p53 both in vitro and in vivo [99], it also binds and deubiquitinates MDM2 in a p53-independent manner and decreasing p53 levels in normal cells [20, 99, 100]."
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"Inhibition of USP7 is expected to increase p53 levels, leading to anti-tumour activity [79], [80]."
26852935
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"Inhibition of USP7 in tumors with wild-type p53 could result in elevation of p53 protein levels through destabilization of MDM2."
28587923
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"However, depletion of HAUSP did not decrease cellular p53 levels, as predicted, but instead led to an increase in p53 levels."
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"In addition, we figure out that usp7 knockdown attenuates Shh pathway, while does not alter P53 protein level and P53 target gene expression."
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"Thus, one role of USP7 in healthy cells is to limit p53-dependent expression of pro-apoptotic BCL-2 family members."
35608338
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"Thus, HAUSP-mediated de-ubiquitination can bring about increased levels of MDM2 that then accelerate p53 degradation to directly reduce the level of p53."
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"Knockdown of USP7 expression increases p53 expression and inhibits colon cancer cells proliferation (Colland et al., 2009)."
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"However, double silencing of USP7 and FAM188B restored the p53 level."
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"Among them, it interacts with USP7 and confers a protective effect from apoptotic challenge on virus-infected cells by lowering p53 levels [39] ."
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"USP7 can increase the level of MDM2, the negative regulatory protein of the tumor suppressor gene p53, and then decrease the level of p53 [13]."
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"It’s widely believed that USP7 impairs p53 level via MDM2, but meanwhile, USP7 can remove ubiquitin off P53 in a direct manner."
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"Overexpression of USP7 completely suppressed the expression of p53 and its inhibitory effect on PRMT1 in A549 cells (Fig. 5N)."
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"However depletion of HAUSP in cells does not decrease p53 levels as predicted, but rather increases p53 levels, apparently due to HAUSP 's ability to bind and deubiquitinate Mdm2."
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