IndraLab

"USP13 can deubiquitinate RAP80 (receptor-associated protein 80) and promote the recruitment of the RAP80-BRCA1 complex to damage sites, fine-tuning the DNA repair system ."

"These results suggest that deubiquitination of RAP80 by USP13 following DNA damage facilitates the binding between RAP80 and K63- linked polyubiquitin chain."

"Deubiquitination of RAP80 by USP13 plays an important role in the ability of RAP80 to bind polyubiquitin, which is important for RAP80 recruitment and DDR."

"Our results suggest that USP13 deubiquitinates RAP80 following DNA damage, which in turn facilitates RAP80 recruitment to DSBs."

"USP13, in turn, deubiquitinates RAP80 and promotes RAP80 recruitment and proper DDR."

"USP13 interacts with and deubiquitinates RAP80."

"USP13 deubiquitinates receptor associated protein 80 (RAP80) and promotes DNA damage response."

"USP13 deubiquitinates RAP80, regulating RAP80-BRCA1 foci formation (55)."

"Since USP13 deubiquitinates RAP80 and regulates DDR, we next examined the role of USP13 in cancer."

"Also, USP13 interacts to and deubiquitylates receptor-associated protein 80 (RAP80) following DNA damage to activate its ability, promoting DNA-damage responses ."

"In contrast, USP13 directly binds and deubiquitylates PTEN to suppress tumorigenesis and glycolysis in PTEN-positive breast cancer cells (35)."

"Deubiquitylation and stabilization of PTEN by USP13."

"In addition to its oncogenic roles, USP13 exerts a tumor-suppressive role by deubiquitinating PTEN in different types of cancers."

"Wild-type USP13 purified from either bacteria or 293T cells, but not its catalytically inactive mutant C345A, decreased PTEN poly-ubiquitination by 64-70% in vitro (XREF_FIG)."

"On the other hand, ectopic expression of wild-type USP13, but not the C345A mutant which is still capable of interacting with PTEN (XREF_FIG), reduced the poly-ubiquitination of PTEN by 65% (XREF_FIG), suggesting that the enzymatic activity of USP13 is indispensable for USP13 dependent deubiquitination of PTEN."

"For example, USP13 prevents tumor cell growth by deubiquitinating PTEN in breast cancer, OSCC and bladder cancer."

"Therefore, USP13 can directly deubiquitinate PTEN."

"PTEN is deubiquitinated by USP13 in bladder cancer, and its stabilized expression suppresses tumor progression (127)."

"PTEN is ubiquitinated by WWP2 and NEDD4 [ 49 , 50 ] and is deubiquitinated by USP13 and OTUD3 [ 19 , 20 ]."

"Deubiquitination of PTEN by USP13, a deubiquitinating enzyme, stabilized PTEN, and thereby inhibited breast cancer tumorigenesis [5]."

"On the other hand, ectopic expression of wild-type USP13, but not the C345A mutant which is still capable of interacting with PTEN (XREF_FIG), reduced the poly-ubiquitination of PTEN by 65% (XREF_FIG), suggesting that the enzymatic activity of USP13 is indispensable for USP13 dependent deubiquitination of PTEN."

"While A20 inhibits PtdIns3P signaling by removing the TRAF6-dependent Lys63-linked chains from <span class="match term1">BECLIN 1</span>, the enzymes USP10 and <span class="match term0">USP13</span> prevent PI3K-III complex components from their degradation and, therefore, support autophagy. Interestingly enough, USP10 also stabilizes p53, which, in turn, triggers the degradation of <span class="match term1">BECLIN 1</span> and VPS34 in order to prevent autophagy."

"Likewise, USP13, which deubiquitinates BECN1 and stabilizes PIK3C3 complexes, is amplified in LUSQ."

"Furthermore, the presence of spautin-1 inhibited the deubiquitination of Beclin1 mediated by USP10 and USP13."

"It promotes the degradation of Vps34 complexes by inhibiting USP10 and USP13, two ubiquitin specific peptidases that target the deubiquitination of Beclin-1."

"In addition, USP10 or USP13 can deubiquitinate and stabilize Beclin 1 in promoting autophagy ."

"Specific and potent autophagy inhibitor-1 (Spautin-1) was identified to inhibit USP10 and USP13, which deubiquitinate the Beclin 1 subunit of Vsp34 complex, and thus promoted the degradation of Vsp34 PI3 kinase complex."

"We demonstrate that USP10 and USP13 can both mediate the deubiquitination of Beclin1."

"Recent studies indicated that USP13 can deubiquitinate and regulate protein levels of Beclin-1, microphathalmia associated transcription factor, Siah2, phosphatase and tensin homolog, and STAT-1, and the deubiquitination process of USP13 could also be orchestrated by Beclin-1."

"In 2011, Yuan et al. found that USP13 could interact with the C-terminal domain of Beclin-1 subunit in the VPS34 complex and deubiquitinate Beclin-1, thereby enhancing the stability of the VPS34 complex, which would contribute to the formation of autophagosomes (Liu et al., 2011)."

"Conversely, it was show that Beclin 1 is deubiquitinated by USP10 and USP13 and adding complexity, Beclin 1 itself controlled the protein stabilities of USP10 and USP13 by regulating their deubiquitinating activities, in turn regulating the levels of tumor suppressor p53 [XREF_BIBR]."

"USP10 and USP13 can deubiquitinate polyubiquitinated beclin-1 to induce autophagy [43]."

"In cholangiocarcinoma, TGF-β signaling triggers the phosphorylation of CLK3, a serine/threonine kinase that directly phosphorylates USP13 at Y708 and facilitates USP13 interaction with c-Myc (Zhou et al., 2020); in GSCs, USP13 can enhance the stability through deubiquitinating c-Myc, activating purine synthesis mediated by c-Myc and inducing the tumorigenesis of GSCs (Fang et al., 2017); in hepatocellular carcinoma, knockdown of USP13 by shRNA can markedly downregulate c-Myc expression, resisting xenograft tumor growth of HCC (Huang et al., 2020)."

"The ubiquitination assay demonstrated that disruption of USP13 by shRNA markedly increased c-Myc ubiquitination and reduced c-Myc protein level in GSCs (XREF_FIG)."

"Furthermore, USP13 can deubiquitinate and stabilise ACLY and OGDH and c-Myc in ovarian cancer and glioblastoma, respectively, thereby functioning as an oncogene [XREF_BIBR, XREF_BIBR]."

"Our data have demonstrated that USP13 mediates deubiquitination of c-Myc, whereas FBXL14 facilitates c-Myc ubiquitination in glioma cells."

"It has been reported that c-Myc can be de-ubiquitinated by USP13 [33]."

"Moreover, USP13 knockdown increased c-Myc ubiquitination, whereas FBXL14 knockdown reduced c-Myc ubiquitination (XREF_FIG)."

"In this study, we demonstrate that the deubiquitinase <span class="match term0">USP13</span> stabilizes c-<span class="match term1">Myc</span> by antagonizing FBXL14-mediated ubiquitination to maintain GSC self-renewal and tumorigenic potential."

"In this study, we demonstrate that the deubiquitinase USP13 stabilizes c-Myc by antagonizing FBXL14-mediated ubiquitination to maintain GSC self-renewal and tumorigenic potential. USP13 was preferentially expressed in GSCs, and its depletion potently inhibited GSC proliferation and tumor growth by promoting c-Myc ubiquitination and degradation."

"In these tumors, USP13 deubiquitinates and stabilizes some oncogenes, including MCL1 [19, 20], Myc [8], MITF [21] and ZHX2 [22]."

"Overexpression of USP13 reduced c-Myc ubiquitination, and forced expression of FBXL14 increased c-Myc ubiquitination (XREF_FIG)."

"Importantly, overexpression of USP13 together with FBXL14 abolished the increased c-Myc ubiquitination caused by FBXL14 overexpression (XREF_FIG, last lane)."

"Consistently, overexpression of the wild type but not the USP13 mutant reduced c-Myc ubiquitination and resulted in elevated c-Myc protein levels (XREF_FIG)."

"As USP13 functions as a deubiquitinase that also interacts with c-Myc (XREF_FIG) and the preferential expression of USP13 in GSCs positively regulates c-Myc protein levels (XREF_FIG), we hypothesized that USP13 might mediate deubiquitination of c-Myc protein to prevent its degradation and stabilize c-Myc in GSCs."

"USP13 promotes deubiquitination of ZHX2 and tumorigenesis in kidney cancer."

"However, a deubiquitinase USP13 inhibits the ubiquitination of ZHX2 and enhances its stability (15)."

"Next, we tried to examine whether USP13 can act as a DUB for ZHX2 and promote ZHX2 deubiquitination and protein stability in ccRCC cells."

"Under denaturing conditions, we found that USP13 WT overexpression led to decreased ZHX2 ubiquitination in ccRCC cell lines (UMRC-2 and 786-O) (Fig. 1M)."

"Interestingly, USP13 has also been implicated in renal cancer, but rather than altering HIF activity, USP13 deubiquitinates another VHL target gene and oncogenic driver, ZHX2 [141]."

"In these two cells, USP13 depletion by two USP13 short hairpin RNA increased the ZHX2 ubiquitination level (Fig. 2 J and K)."

"In conclusion, our results suggest that USP13 depletion down-regulated the ZHX2 level by increasing ZHX2 ubiquitination and degradation."

"In addition, USP13 promotes tumorigenesis of clear cell renal cell carcinoma through deubiquitinating ZHX2 ."

"19 Similarly, the process of ZHX2 deubiquitination catalyzed by USP13 is the committed step in the tumorigenesis of ccRCC."

"It was found that USP13 could deubiquitinate zinc fingers and homeoboxes 2 (ZHX2) and stabilize ZHX2 protein."

"Another study has shown that USP13 is essential for HPV-positive cervical cancer cells to proliferate, at least in part by deubiquitinating and stabilizing the prosurvival protein Mcl-1 [122]."

"Therefore, USP13 interacted with and deubiquitinated MCL1 to preserve its protein stability."

"USP13 interacts with MCL1 and stabilizes its expression by deubiquitinating MCL1."

"Collectively, we nominate <span class="match term0">USP13</span> as a novel deubiquitinase which regulates <span class="match term1">MCL1</span> turnover in diverse solid tumors and propose that <span class="match term0">USP13</span> may be a potential therapeutic target for the treatment of various malignancies."

"Morgan et al. (2021) demonstrated that USP13 deubiquitinates and stabilizes Mcl-1, promoting the proliferation in cervical cancer."

"In these tumors, USP13 deubiquitinates and stabilizes some oncogenes, including MCL1 [19, 20], Myc [8], MITF [21] and ZHX2 [22]."

"Morgan et al. further revealed that USP13 enhances cervical cancer cell growth via the deubiquitination of Mcl-1 [34]."

"USP13, on the other hand, can deubiquitinate and stabilize MCL1, which is not sensitive to MCL-2 family inhibitors, and render tumor cells highly resistant to BH3-type chemotherapy drugs (Oltersdorf et al., 2005; Delbridge et al., 2016; Kotschy et al., 2016)."

"USP13 interacts with and deubiquitinates Mcl-1 in cervical cancer cells."

"Next, we confirmed that USP13 deubiquitinated Mcl-1."

"As previously mentioned, USP10 mediates the deubiquitination of Beclin1, and USP13 can directly regulate the deubiquitination of USP10 to promote the formation of autophagosomes ."

"A previous study indicated that USP13 affects p53 by deubiquitinating USP10 [33]."

"Consistent with this possibility, the ubiquitination levels of USP10 were reduced when cells were cotransfected with an expression vector of USP13 and the addition of spautin-1 inhibited the deubiquitination of USP10 by USP13 (XREF_FIG)."

"These results suggest that USP13 may directly regulate the deubiquitination of USP10; however, USP10 may regulate USP13 indirectly perhaps by affecting the levels of Vps34 complexes."

"Moreover, beclin-1 and USP10 are involved in a potential feedforward mechanism in which beclin-1 stabilizes USP13, which in turn deubiquitinates and stabilizes USP10, leading to increased beclin-1 levels and activity."

"Since USP13 can also deubiquitinate USP10, regulating the stability of USP13 by Beclin1 provides a mechanism for Beclin1 to control the stability of USP10."

No evidence text available

"Very interestingly, they also pointed out that beclin-1 controlled the stability of USP10 by regulating the stability of USP13, which can deubiquitinate USP10 (also see Figure 4) [53]."

"USP13 has been found to stabilize p53, a typical tumor suppressor by de-ubiquitinating its de-ubiquitinating protease USP10 [24]."

"USP13 directly deubiquitinates ACLY and OGDH."

"In ovarian tumors, upregulation of USP13 enhances deubiquitination and stabilization of ACLY (ATP citrate lyase) and OGDH (oxoglutarate dehydrogenase), two key enzymes that drive glutaminolysis, ATP generation, and lipid synthesis in cancer metabolism , and MCL1, a pivotal member of the antiapoptotic BCL-2 family of proteins ."

"Furthermore, USP13 can deubiquitinate and stabilise ACLY and OGDH and c-Myc in ovarian cancer and glioblastoma, respectively, thereby functioning as an oncogene [XREF_BIBR, XREF_BIBR]."

"<span class="match term0">USP13</span>, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

"As it was aforementioned, USP13 deubiquitinates OGDH and ACLY."

"Taken together, these studies suggest that USP14 is involved in the occurrence and progression of multiple malignant tumors.Other USP family members: USP13, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

"USP13 influences cell cycle progression in gastric cancer by stabilizing cyclin D1 [15] and enhances the deubiquitination and stabilization of ATP citrate lyase (ACLY) and oxoglutarate dehydrogenase (OGDH) in ovarian tumors [16]."

"Mechanistically, USP13 promoted the energy metabolism of tumor cells, and provided precursor substances for the synthesis of sugar, lipids and non-essential amino acids in cancer cells, through deubiquitinating and stabilizing ACLY and OGDH (Han et al., 2016)."

"The C345A mutant of USP13 failed to reduce the ubiquitination of ACLY (XREF_FIG), suggesting that the deubiquitination activity is essential for the function of USP13."

"Mechanistically, USP13 promoted the energy metabolism of tumor cells, and provided precursor substances for the synthesis of sugar, lipids and non-essential amino acids in cancer cells, through deubiquitinating and stabilizing ACLY and OGDH (Han et al., 2016)."

"Furthermore, USP13 can deubiquitinate and stabilise ACLY and OGDH and c-Myc in ovarian cancer and glioblastoma, respectively, thereby functioning as an oncogene [XREF_BIBR, XREF_BIBR]."

"As it was aforementioned, USP13 deubiquitinates OGDH and ACLY."

"Taken together, these studies suggest that USP14 is involved in the occurrence and progression of multiple malignant tumors.Other USP family members: USP13, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

"<span class="match term0">USP13</span>, the main regulator of ovarian cancer energy metabolism, specifically deubiquitinates and stabilizes oxoglutarate dehydrogenase and ATP citrate lyase, which can catalyze fatty acid synthesis, glutaminolysis and mitochondrial respiration."

"USP13 influences cell cycle progression in gastric cancer by stabilizing cyclin D1 [15] and enhances the deubiquitination and stabilization of ATP citrate lyase (ACLY) and oxoglutarate dehydrogenase (OGDH) in ovarian tumors [16]."

"USP13 directly deubiquitinates ACLY and OGDH."

"In ovarian tumors, upregulation of USP13 enhances deubiquitination and stabilization of ACLY (ATP citrate lyase) and OGDH (oxoglutarate dehydrogenase), two key enzymes that drive glutaminolysis, ATP generation, and lipid synthesis in cancer metabolism , and MCL1, a pivotal member of the antiapoptotic BCL-2 family of proteins ."

"In contrast, overexpression of USP13 significantly decreased ubiquitination of METTL3 in HOS cells (Fig. S4a)."

"Overexpression of WT USP13 decreased METTL3 ubiquitination while C345A mutant USP13 showed no significant effect (Fig. 4b)."

"USP13 was shown to positively promote glycolysis and tumor progression by stabilizing and deubiquitinating METTL3, which is a well-known “writer” for m A modification, at K488 by removing K48-linked ubiquitin chains."

"In this study, we found that USP13 interacts with METTL3 and decreases METTL3 ubiquitination, resulting in reduced METTL3 degradation in OS."

"Depletion of USP13 significantly promoted METTL3 ubiquitination level compared with shNC in 143B cells (Fig. 4a)."

"Ubiquitin-specific proteases 13 (USP13) regulates glycolytic reprogramming and proliferation in osteosarcoma by deubiquitinating METTL3 at K488."

"Our results indicated that USP13 promotes glycolytic reprogramming and progression in OS by stabilizing as well as deubiquitinating METTL3 protein at K488 by removing K48-linked ubiquitin chains, which further stabilizes ATG5 mRNA and activates oncogenic autophagy."

"USP13 deubiquitinates METTL3 at K488 by removing K48-linked ubiquitin chains."

"The USP family of DUBs is very important, with USP13, USP18, USP20, USP21, USP44, and USP49 shown to mediate the deubiquitination of STING (Chen et al., 2021)."

"Consistently, reconstitution of USP13 but not USP13 (AE) into Usp13 m/m MEFs inhibited HSV-1-induced ubiquitination of STING (XREF_FIG)."

"Here, we show that ubiquitin-specific protease 13 (<span class="match term0">USP13</span>) is a STING-interacting protein that catalyses deubiquitination of <span class="match term1">STING</span>."

"For example, USP13 regulates antiviral responses by deubiquitinating STAT1 and STING (Sun et al. 2017; Yeh et al. 2013) and is related to PTEN and MITF in the modulation of tumorigenesis (Zhang et al. 2013; Zhao et al. 2011)."

"In addition, USP13 negatively modulates antiviral immunity by deubiquitinating STING, and targeting USP13 consequently triggers STING-interferon signaling and strengthens innate immunity [26]."

"As expected, we found that USP13 but not the enzymatic inactive mutant USP13 (AE) catalysed deubiquitination of STING in cells or in vitro (XREF_FIG)."

"For example, USP13 regulates antiviral responses by deubiquitinating STAT1 and STING (Sun et al. 2017; Yeh et al. 2013) and is related to PTEN and MITF in the modulation of tumorigenesis (Zhang et al. 2013; Zhao et al. 2011)."

"We detected that overexpression of USP13 attenuated the ubiquitination of STAT1, reversing the MBTPS1-induced increase in STAT1 ubiquitination, reduction in stability, and subsequent decrease in STAT1 protein levels (Fig. 7i–k)."

"This finding was consistent with the findings of a recent study showing that USP13 deubiquitinates STAT1 ."

"This competition subsequently interfered with the deubiquitination of STAT1 by USP13, promoting its degradation via the ubiquitin‒proteasome pathway."

"Congruently, STAT1 ubiquitination is reduced in cells by USP13 overexpression and increased with USP13 knockdown regardless of IFNα treatment (115)."

"A recent report suggested that the deubiquitinase USP13 decreased STAT1 ubiquitination, which however was independent of IFNs treatment [XREF_BIBR]."

"YY1 induced USP13 transcriptional activation drives the malignant progression of hepatocellular carcinoma by deubiquitinating WWP1."

"USP13 deubiquitinated and regulated WWP1 in a proteasomal-dependent way.."

"As shown in Fig. 4I, the HA-Ub mutants K27R, K48R, and K63R attenuated the deubiquitination of WWP1 by USP13."

"By acting as a DUB, USP13 can bind to and then deubiquitinate and stabilize the WWP1 protein, thus activating the Akt/mTOR pathway."

"Here, we revealed that USP13, which acts as a DUB, deubiquitinates WWP1, which was identified as a new substrate of USP13."

"We found that USP13 de-ubiquitinates Twist1 by binding to the WR region."

"We found that Twist1 can be de-ubiquitinated by USP13."

"USP13 deubiquitinates Twist1."

"USP13 inhibits FBXL14-induced Twist1 ubiquitination."

"USP13, which acts as an oncogene, was shown to promote breast cancer metastasis by deubiquitinating Twist 1 [35]."

"Further evidence showed that EMT might be the primary mechanism for the enhanced metastatic property in GC cells: USP13 and USP29 promoted TGF-β1-induced EMT through interacting with and deubiquitinating Snail, while USP3 facilitated this process by deubiquitinating SUZ12 (Wu et al. 2021; Zhang et al. 2022a, b; Qian et al. 2020)."

"The report from Zhang et al. revealed that USP13 promotes the epithelial-mesenchymal transition (EMT) and metastasis in GC by deubiquitinating and stabilizing Snail in GC [10]."

"The transcriptional activator PLAGL2 activates the ubiquitin-specific peptidase 13 (USP13), which deubiquitinates and maintains Snail to influence the metastatic power of GC cells [64]."

"USP13 interacted with Snail to deubiquitinate and stabilize Snail in GC cells."

"USP13, USP29, and USP37 were reported to promote the metastasis of GC by deubiquitinating and stabilizing Snail [35,36,54,59]."

"Sp-1 functions mainly by inhibiting the activity of USP13, which associates with and deubiquitinates RAF1."

"USP13 deubiquitinates Raf1."

"The abovementioned results indicated that USP13 deubiquitinates Raf1 to increase its stability, while Sp-1 antagonizes USP13 function and promotes Raf1 degradation."

"Finally, the ubiquitination of HA-hRAF1 was examined in FLAG-hUSP13-expressing hiPSCs and the western blot results showed that USP13 suppressed RAF1 ubiquitination (Fig. 8H)."

"Sp-1 mainly functions by inhibiting the deubiquitinating protein USP13, which interacts with and thereby deubiquitinates the Raf1 protein in the cells; the latter is a key component in the activation of MEK/ERK signaling (Fig. 9)."

"In this study, we reported for the first time that USP13 stabilized and deubiquitinated cell cycle protein cyclin D1 in GC."

"USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation."

"To further define whether USP13 deubiquitinated cyclin D1, we transfected USP13 siRNAs or negative control siRNA together with Myc-cyclin D1 and HA-Ub into HEK293T cells and performed Co-IP assays."

"Taken together, our data demonstrate that USP13 deubiquitinates and stabilizes cyclin D1, thereby promoting cell cycle progression and cell proliferation in GC."

"USP13 interacted with and deubiquitinated cyclin D1."

"Treatment with a TXNIP inhibitor, spautin-1, increased TXNIP polyubiquitination (Figure 7B), while overexpression of USP13 decreased TXNIP polyubiquitination (Figure 7C), suggesting USP13 targets and deubiquitinates TXNIP, leading to stabilization of TXNIP.4."

"USP13 deubiquitinates and stabilizes TXNIP in lung fibroblasts."

"This study is the first to reveal that USP13 may deubiquitinate and stabilize TXNIP."

"Mechanistic studies revealed that USP13 targeted and deubiquitinated TXNIP."

"Mechanistically, <span class="match term0">USP13</span> binds to <span class="match term1">TopBP1</span> and stabilizes <span class="match term1">TopBP1</span> by deubiquitination"

"Protein interaction experiments demonstrated that USP13 could co-immunoprecipitate with endogenous TopBP1, and in vitro deubiquitination enzyme experiments showed that WT-USP13 could deubiquitinate TopBP1, while CA-USP13 could not, highlighting the requirement for USP13 ubiquitination activity (Kim et al., 2021)."

"USP13 can deubiquitinate DNA topoisomerase 2 binding protein 1 (TopBP1), influencing DNA chain breakage and repair processes."

"For instance, studies have shown that TopBP1 is ubiquitinated by the E3 ubiquitin ligase hHYD and deubiquitinated by USP13, while its phosphorylation enhances its recruitment to DNA damage sites [18, 19]."

"Moreover, spautin-1, is another effective inhibitor, which impedes the initiation step of autophagy, by suppressing the crucial for the process ubiquitin-specific peptidases USP13, USP10, as well as Beclin-1, which is deubiquitinated in Vps34 complex[99]."

"Meanwhile, an in vitro deubiquitination assay showed that USP13 inhibits the K48linked ubiquitination of PIK3C3 in the presence of NEDD4."

"Interestingly, NEDD4-1 undergoes auto-ubiquitination that serves it as a scaffold for engaging the ubiquitin-specific protease 13 (USP13) to form a NEDD4-1/USP13 deubiquitination complex, which subsequently deubiquitinates and stabilizes VPS34 to induce phagophore nucleation in cancer cells."

"Auto-ubiquitination of NEDD4-1 Recruits <span class="match term0">USP13</span> to Facilitate Autophagy through Deubiquitinating <span class="match term1">VPS34</span>"

"This study is the first to report that USP13 is involved in aging by deubiquitinating MDM2."

"Considering the direct association between USP13 and MDM2 and the role of USP13 in the degradation of MDM2, we examined if USP13 deubiquitinates MDM2."

"The results indicate that USP13 deubiquitinates MDM2."

"We also used in vitro experiments to examine the deubiquitination of MDM2 by USP13 using a purified human recombinant protein USP13 (rhUSP13) and MDM2 autoubiquitination kit."

"USP13 deubiquitinates HMGB1."

"Collectively, our results suggest that USP13 deubiquitinates HMGB1 to maintain its stability and is a key molecule that regulates HMGB1 secretion (Fig. 7)."

"Subsequently, we tested whether USP13 could deubiquitinate HMGB1."

"We demonstrated that USP13 deubiquitinates and stabilizes HMGB1."

"In more detail, Gao et al. demonstrated that USP13 potentiated the deubiquitination and the stabilization of TLR4, activating the TLR4/MyD88/NF-κB pathway under hypoxic conditions."

"Hypoxia-induced ubiquitin-specific peptidase 13 (USP13) deubiquitinated and stabilized toll-like receptor 4 (TLR4) to activate the myeloid differentiation primary response gene 88/nuclear factor-κB (MyD88/NF-κB) pathway, thereby contributing to HCC progression (Gao et al., 2020)."

"USP13 knockdown increases TLR4 ubiquitination, leading to its decreased expression, which results in the decreased expression of MYD88, phosphorylated-NF-κB and p65, thus inhibiting the proliferation and invasion of HCC cells."

"The report from Zhang et al. revealed that USP13 promotes the epithelial-mesenchymal transition (EMT) and metastasis in GC by deubiquitinating and stabilizing Snail in GC [10]."

"USP13 interacted with Snail to deubiquitinate and stabilize Snail in GC cells."

"In this study, we reported for the first time that USP13 stabilized and deubiquitinated cell cycle protein cyclin D1 in GC."

No evidence text available

"We reported here that USP2 is a new deubiquitylase of SKP2 E3 ligase, which interestingly stabilized both SKP2 and SKP2 substrates.Two DUBs, USP10 and USP13, were previously reported to deubiquitylating SKP2."

"On the other hand, USP10 (28) and USP13 (29) were reported to promote SKP2 deubiquitylation."

"We have shown that deubiquitination of Sigirr by USP13 increased Sigirr half-life (L. Li et al., 2019)."

"The ubiquitination and degradation of Sigirr is negatively regulated by a deubiquitinase, USP13 (L. Li et al., 2019)."

"The deubiquitinase <span class="match term0">USP13</span> stabilizes the anti-inflammatory receptor IL-1R8/<span class="match term1">Sigirr</span> to suppress lung inflammation"

"We found that nsp13 interacts with the deubiquitinase USP13, which deubiquitinates and stabilizes nsp13."

"Loss of USP13 enhances ubiquitination of nsp13 and destabilizes nsp13 protein."

"we identify <span class="match term0">USP13</span> as a gp78-associated DUB that eliminates ubiquitin conjugates from <span class="match term1">Ubl4A</span> to maintain the functionality of Bag6"

"USP13 and gp78 control ubiquitination of Ubl4A.These data suggest that USP13 and gp78 play antagonizing roles in regulation of Ubl4A ubiquitination: While gp78 assembles ubiquitin chains on Ubl4A, USP13 antagonizes this activity to limit Ubl4A ubiquitination.Ubiquitination of Ubl4A preferentially occurs on Lys48. We identify the Bag6 cofactor Ubl4A as a shared substrate of gp78 and USP13. USP13 depletion is associated with hyper-ubiquitination of Ubl4A and altered interaction between the Bag6 complex and its co-chaperone SGTA. Because the interaction of Ubl4A with SGTA is mediated by positively-charged residues in Ubl4A including Lys48 (Chartron et al., 2012; Xu et al., 2012), which happens to be the major ubiquitination site, the simplest model to explain reduced Bag6-SGTA interaction in USP13 knockdown cells is that ubiquitin conjugates on Ubl4A sterically hinder SGTA binding."

"USP13 deubiquitinates p62/SQSTM1 to induce autophagy and Nrf2 release for activating antioxidant response genes."

"USP13 deubiquitinates p62/SQSTM1 to induce autophagy and Nrf2 release for activating antioxidant response genes."

"Knockdown of USP13 decreased Smad4 half-life and promoted Smad4 ubiquitination."

"USP13 stabilizes Smad4 by deubiquitinating Smad4.."

"The DUB USP13 regulates Siah2 availability and activity as deubiquitination of Siah2 by USP13 results in a more stable, albeit less active, ubiquitin ligase."

"Besides phosphorylation, Siah2 activity can be modulated by the deubiquitinating enzyme USP13, which binds to and deubiquitinates Siah2, increasing its stability but diminishing its activity toward its substrates."

"Further investigation revealed that USP13 deubiquitinated SARM1, which increased the inhibitory interaction between the N-terminal armadillo repeat motif (ARM) and C-terminal Toll/interleukin-1 receptor (TIR) domains of the SARM1 protein, thereby suppressing SARM1 activation in axon injury."

"Deubiquitination of SARM1 by USP13 regulates SARM1 activation and axon degeneration."

"We subsequently determined that USP13 significantly reduced the polyubiquitination of PRPF6, with a specific focus on the K48 and K63 chain linkages (Figures 5D,E)."

"The results above indicate that USP13 deubiquitinates PRPF6, thereby stabilising its protein levels and promoting the proliferation of HCC cells via modulation of the AKT‐mTOR signalling pathway."

"Second, USP13 knockdown can increase Parkin ubiquitination and activity, thereby reducing the level of α-Syn; the excessive expression of USP13 increases the expression of α-Syn [68,69]."

"Here, it was shown that USP13 negatively regulates Parkin ubiquitination, function, and solubility (Figure 5), negatively regulates the level, ubiquitination, and clearance of α-synuclein, and regulates proteasome activity independently of Parkin, which affects dopaminergic neuronal viability and motor performances in animal models."

"However, deubiquitinase <span class="match term0">USP13</span> can stabilize <span class="match term1">MITF</span> expression and prolong its half-life."

"In these tumors, USP13 deubiquitinates and stabilizes some oncogenes, including MCL1 [19, 20], Myc [8], MITF [21] and ZHX2 [22]."

"Because ectopic overexpression of USP13 promoted deubiquitination of cohesin subunits, inactivation of USP13 was conversely expected to cause enhanced accumulation of ubiquitinated cohesin subunits."

"To investigate whether USP13 mediated the deubiquitination of cohesin subunits, we cotransfected 293T cells with expression vectors for His ubiquitin and Myc-USP13, purified ubiquitinated proteins by Ni-NTA affinity chromatography, and performed Western blot with antibodies to SMC3 and RAD21 (XREF_FIG A)."

"Depletion of USP13 from the substantia nigra (SN), the brain region affected in PD, increased α-synuclein ubiquitylation and degradation."

"In this study, we reported for the first time that USP13 stabilized and deubiquitinated cell cycle protein cyclin D1 in GC."

"Interestingly, USP13 has also been implicated in renal cancer, but rather than altering HIF activity, USP13 deubiquitinates another VHL target gene and oncogenic driver, ZHX2 [141]."

"Future studies are warranted to determine the ubiquitination of vinculin in 293T cell lines and whether USP13 deubiquitinates vinculin polyubiquitylation and stabilizes vinculin protein."

"These results suggest that PARP1 is a potential substrate of USP13, which can be directly deubiquitinated by USP13 to promote its protein stability in MM.3.5 USP13/PARP1 Axis Positively Regulates MM Cell Proliferation by Activating DDR."

"Interestingly, Beclin 1 can also modulate p53 protein stability by regulating the activities of USP10/USP13, which can deubiquitinate p53 ."

"Although our results have not ruled out the possibility that USP13 may contribute to deubiquitination of TCRalpha with the assistance of a substrate recruiting adaptor in cells, the inability to deubiquitinate TCRalpha by purified USP13 suggests that the accumulation of ubiquitinated TCRalpha in USP13 knockdown cells is probably due to ERAD inhibition at a step downstream of ubiquitination, as shown in Bag6 depleted cells."

"For example, USP13 regulates antiviral responses by deubiquitinating STAT1 and STING (Sun et al. 2017; Yeh et al. 2013) and is related to PTEN and MITF in the modulation of tumorigenesis (Zhang et al. 2013; Zhao et al. 2011)."

"Protein-protein interactions assays showed that USP13 interacted with the co-immunoprecipitated protein spleen tyrosine kinase (SYK) and deubiquitinated SYK."

"In addition, USP13 has been reported to regulate the cellular antiviral response by deubiquitinating STING28."

"USP13 has been found to stabilize p53, a typical tumor suppressor by de-ubiquitinating its de-ubiquitinating protease USP10 [24]."

"Further ubiquitination experiments, after treatment with 20 μM MG132, demonstrated that increased expression of USP13 can enhance the deubiquitination of PARP1 protein, leading to reduced degradation (Figure 4E,F)."

"Moreover, we demonstrated that <span class="match term0">USP13</span> is a key enzyme for Paxillin-mediated <span class="match term1">NLRP3</span> deubiquitination upon ATP treatment"

"USP13 interacts with TAK1, inhibits TAK1 activation by removing ubiquitination of TAK1, and subsequently inhibits the NF-κB & MAPK signaling pathway activation (Fig. 9), thereby treating NAFLD as a latent molecular target.As a DUB enzyme, USP13 regulates the de-ubiquitination of multiple substrate proteins to participate in many cellular processes, including mitochondrial energy metabolism, DNA damage, autophagy, and endoplasmic reticulum-associated degradation [35]."

"We demonstrated that USP13 interacts with and deubiquitinates β-catenin, which promotes NSCLC metastasis."

"USP13 regulates ferroptosis in chicken follicle granulosa cells by deubiquitinating ATG7."

"Here, we identified USP13 as an essential deubiquitinase that stabilizes ATG5 in a process that depends on the PAK1 serine/threonine-protein kinase and which enhances autophagy and promotes IM resistance in GIST cells."

"The proper function of BAG6 seems to require the activity of the deubiquitinating enzyme USP13, which antagonizes gp78 mediated ubiquitination to ensure ERAD efficiency."