IndraLab

Statements

VCPIP1 is modified

28 | 76 59

VCPIP1 is phosphorylated.

27 | 69 59

VCPIP1 is phosphorylated. 10 / 76

| 58 18

➶

sparser

"In addition to its S130 phosphorylation, VCIP135 is also phosphorylated at multiple sites at the C-terminus, which regulates VCIP135 membrane association and p97 interaction ( xref )."

25904330

➶

sparser

"These results suggest that VCPIP1 is phosphorylated by ATM/ATR following DPC induction."

32649882

➶

sparser

"Our in vitro Golgi reassembly assay confirmed that VCIP135 phosphorylation at S130 attenuates p97/p47-mediated Golgi membrane fusion ( xref )."

25904330

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sparser

"Given our results, we propose that VCIP135 phosphorylation on S130 in early mitosis inhibits its activity and blocks Golgi membrane fusion, resulting in mitotic Golgi fragmentation; while VCIP135 reactivation by S130 dephosphorylation in telophase initiates p97/p47-mediated Golgi membrane fusion for postmitotic Golgi reassembly."

25904330

➶

rlimsp

"VCIP135 is phosphorylated at multiple sites during mitosis (Totsukawa ; Zhang ), but it was not clear whether and how VCIP135 phosphorylation regulates its DUB activity and function in Golgi membrane fusion."

25904330

➶

sparser

"As presented in Fig. 2 A, Cdc2-mediated VCIP135 phosphorylation inhibited its binding to GST-p97 (upper panel, lane 2)."

23500464

➶

rlimsp

"Mitotic phosphorylation of VCIP135 blocks p97ATPase-mediated Golgi membrane fusion."

23500464

➶

sparser

"These results demonstrate that VCIP135 is phosphorylated in mitosis on S130 and is dephosphorylated in later mitosis."

25904330

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sparser

"Taken together, our results demonstrate that phosphorylation of VCIP135 on S130 in early mitosis inactivates its DUB activity for mitotic Golgi disassembly; dephosphorylation in telophase reactivates VCIP135 to promote p97/p47-mediated postmitotic Golgi membrane fusion."

25904330

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sparser

"Mitotic phosphorylation of VCIP135 at S130 inhibits its deubiquitinase activity."

25904330

VCPIP1 is phosphorylated on S131. 10 / 40

| 40

➶

rlimsp

"Phosphorylation of VCIP135 at S130 attenuates p97/p47-mediated Golgi membrane fusion."

25904330

➶

rlimsp

"VCIP135 is phosphorylated on multiple sites at the C-terminus in addition to S130 (Totsukawa ; Zhang ), and a total of 13 sites on VCIP135 are phosphorylated in mitosis (Supplemental Figure 1)."

25904330

➶

rlimsp

"The DUB activity was low when VCIP135 was highly phosphorylated at S130 in early mitosis (Figure 4C, lanes 2–4); while the DUB activity increased significantly when S130 was dephosphorylated in late mitosis (Figure 4C, lanes 5–7)."

25904330

➶

rlimsp

"Because VCIP135 DUB activity is specifically required for postmitotic Golgi membrane fusion, we determined the effects of S130 phosphorylation of VCIP135 on postmitotic Golgi reassembly in vivo."

25904330

➶

rlimsp

"Taken together, these results demonstrate that S130 phosphorylation negatively regulates VCIP135 activity, which controls Golgi structure formation during the cell cycle."

25904330

➶

rlimsp

"We have developed a phospho-antibody that specifically recognizes phosphorylated S130 on VCIP135 (Figure 3). Biochemical (Figure 4) and microscopy (Figures 4 and 5) studies showed that S130 is phosphorylated from early mitosis to anaphase, which inactivates VCIP135 DUB activity."

25904330

➶

rlimsp

"Mitosis-arrested cells were obtained by treatment with 100 ng/ml nocodazole (Sigma-Aldrich, St. Louis, MO) for 18 h. Mitotic-arrested cells were then washed with fresh medium without nocodazole five times; placed into new dishes; and incubated for another 0.5, 1, 1.5, 2, and 3 h. For determination of the effects of kinase inhibitors on VCIP135 S130 phosphorylation and VCIP135 DUB activity, nocodazole-arrested mitotic cells were treated with 2 μM staurosporine for 45 min or with 20 μM roscovitine or 20 μM U0126 for 2 h, respectively (Zhang )."

25904330

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rlimsp

"We next determined the kinase responsible for VCIP135 S130 phosphorylation in mitosis. Because S130 matches the minimal S/T-P consensus motif for both Cdk1 (Nigg, 1993) and ERK1/2 (Davis, 1993; Songyang ), we applied inhibitors to these protein kinases to mitotic cells and investigated the effects on S130 phosphorylation of VCIP135. Flag-tagged VCIP135 was immunoprecipitated and analyzed by Western blotting using the pSer-130 antibody (Figure 6A). Treatment of cells with staurosporine (a general serine/threonine kinase inhibitor) or roscovitine (a Cdk inhibitor) abolished the pSer130 signal. In contrast, treatment of cells with U0126 (a MEK inhibitor that inhibits ERK1/2 activation) had no effect on S130 phosphorylation."

25904330

➶

rlimsp

"As a key regulator of Golgi membrane fusion, the activity of VCIP135 is tightly controlled by phosphorylation at S130 during the cell cycle progression. We have developed a phospho-antibody that specifically recognizes phosphorylated S130 on VCIP135 (Figure 3)."

25904330

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rlimsp

25904330

VCPIP1 is phosphorylated on T761. 7 / 7

2 | 5

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sparser

"Our results indicate that the phosphorylation of VCIP135 on Threonine-760 and Serine-767 inhibits p97-mediated Golgi membrane fusion at mitosis."

23500464

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hprd

No evidence text available

19413330

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sparser

"All these results indicate that phosphorylation of Threonine-760 and Serine-767 in VCIP135 inhibits its binding to p97."

23500464

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sparser

"Taken together, these results demonstrate that the phosphorylation of Threonine-760 and Serine-767 in VCIP135 inhibits both p97-mediated Golgi reassembly pathways, indicating that it is important for [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23500464

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hprd

No evidence text available

18669648

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sparser

"We established that VCIP135 is mitotically phosphorylated at Threonine-760 and Serine-767 and that the phosphorylated VCIP135 cannot bind to p97."

23500464

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sparser

"In summary, our biochemical data show that phosphorylation of Threonine-760 and Serine-767 in VCIP135 inhibits its binding to p97, but does not affect its binding to WAC nor its deubiquitinating activ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23500464

VCPIP1 is phosphorylated on S768. 6 / 6

| 5 1

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sparser

23500464

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sparser

"Our results indicate that the phosphorylation of VCIP135 on Threonine-760 and Serine-767 inhibits p97-mediated Golgi membrane fusion at mitosis."

23500464

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sparser

23500464

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sparser

"All these results indicate that phosphorylation of Threonine-760 and Serine-767 in VCIP135 inhibits its binding to p97."

23500464

➶

sparser

"We established that VCIP135 is mitotically phosphorylated at Threonine-760 and Serine-767 and that the phosphorylated VCIP135 cannot bind to p97."

23500464

➶

rlimsp

"Our results indicate that the phosphorylation of VCIP135 on Threonine-760 and Serine-767 inhibits p97-mediated Golgi membrane fusion at mitosis."

23500464

VCPIP1 is phosphorylated on S1198. 3 / 3

3 |

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hprd

No evidence text available

19651622

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hprd

No evidence text available

18669648

➶

hprd

No evidence text available

20068231

VCPIP1 is phosphorylated on S994. 3 / 3

3 |

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hprd

No evidence text available

19651622

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hprd

No evidence text available

20068231

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hprd

No evidence text available

18669648

VCPIP1 is phosphorylated on S747. 3 / 3

3 |

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hprd

No evidence text available

19413330

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hprd

No evidence text available

15302935

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hprd

No evidence text available

18669648

VCPIP1 is phosphorylated on S997. 2 / 2

2 |

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hprd

No evidence text available

20068231

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hprd

No evidence text available

19651622

VCPIP1 is phosphorylated on S1132. 2 / 2

2 |

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hprd

No evidence text available

20068231

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hprd

No evidence text available

18669648

VCPIP1 is phosphorylated on Y767. 2 / 2

2 |

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hprd

No evidence text available

19413330

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hprd

No evidence text available

18669648

VCPIP1 is phosphorylated on S757. 2 / 2

2 |

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hprd

No evidence text available

18669648

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hprd

No evidence text available

19413330

VCPIP1 is phosphorylated on S998. 2 / 2

2 |

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hprd

No evidence text available

18669648

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hprd

No evidence text available

19651622

VCPIP1 is phosphorylated on S1000. 1 / 1

1 |

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hprd

No evidence text available

20068231

VCPIP1 is phosphorylated on T763. 1 / 1

1 |

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hprd

No evidence text available

18669648

VCPIP1 is phosphorylated on S1128. 1 / 1

1 |

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hprd

No evidence text available

20068231

VCPIP1 is phosphorylated on S756. 1 / 1

1 |

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hprd

No evidence text available

18669648

VCPIP1 is phosphorylated on S1207. 1 / 1

| 1

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sparser

"Next, we further investigated the importance of S1207 phosphorylation of VCPIP1 during DPC repair."

32649882

VCPIP1 is phosphorylated on Y137. 1 / 1

1 |

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hprd

No evidence text available

20068231

VCPIP1 is phosphorylated on T770. 1 / 1

1 |

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hprd

No evidence text available

18669648

VCPIP1 is dephosphorylated.

| 7

VCPIP1 is dephosphorylated. 7 / 7

| 7

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sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

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sparser

"In mitosis, VCIP135 is phosphorylated at S130 by Cdk1 and thus is inactivated, allowing syntaxin 5 to be ubiquitinated by HACE1; in telophase, VCIP135 is dephosphorylated and reactivated, removing ubiquitin from syntaxin 5 to allow p97-mediated membrane fusion ( xref ; xref )."

31435807

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sparser

31435807

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sparser

"Dephosphorylation of VCIP135 at S130 in telophase activates its deubiquitinase activity for Golgi reassembly."

25904330

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sparser

31435807

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sparser

"Conversely, at the end of mitosis, VCIP135 is dephosphorylated (on Ser130), and this is associated with recovery of its deubiquitinase activity and with post-mitotic Golgi reassembly (Zhang and Wang, xref )."

26734607

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sparser

"Dephosphorylation of S130 on VCIP135 upon staurosporine or roscovitine but not U0126 treatment also significantly increased the DUB activity of endogenous VCIP135 ( xref ), consistent with the hypothesis that phosphorylation at S130 inactivates VCIP135."

25904330

VCPIP1 is acetylated.

1 |

VCPIP1 is acetylated on K408. 1 / 1

1 |

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hprd

No evidence text available

19608861

VCPIP1 affects VCP

6 2 | 31 19

VCPIP1 binds VCP.

6 2 | 24 19

VCP binds VCPIP1. 10 / 33

6 1 | 17 9

➶

biogrid

No evidence text available

23500464

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biogrid

No evidence text available

19615732

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reach

"Although VCIP135 can bind to p97 in cytosol (XREF_FIG A), VCIP135 bound to syntaxin5 does not form a complex with p97 (XREF_FIG C, lane 4)."

12473691

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reach

"In late mitosis, the DUB VCIP135, which associates with p97 via its UBX-L (UBX like) domain, deubiquitinates Syn5, which permits interaction between the SNAREs, membrane fusion and finally the formation of Golgi cisternae [XREF_BIBR]."

28819009

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sparser

"We established that VCIP135 is mitotically phosphorylated at Threonine-760 and Serine-767 and that the phosphorylated VCIP135 cannot bind to p97."

23500464

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biogrid

No evidence text available

21811234

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sparser

"We first tested whether phosphorylated VCIP135 binds to p97."

23500464

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reach

"This provides a reasonable explanation for our biochemical data that p47 (271-370) binds to p97 but not to the p97 and VCIP135 complex (XREF_FIG B)."

12473691

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reach

"Golgi WAC interacts with the deubiquitinase VCIP135, which binds VCP and p97."

26687599

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reach

"Although VCIP135 also binds to p97 [13], it remains unclear whether complex formation between VCIP135 and p97 is really necessary for p97 mediated membrane fusion."

23500464

VCP binds NSFL1C and VCPIP1. 4 / 4

| 3 1

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"The ubiquitin moieties on these substrates, added by HACE1 during mitosis, likely serve as receptors for the p97, p47, and VCIP135 complex at the end of mitosis to carry on fusion reactions after the removal of the ubiquitin tags by VCIP135 (XREF_FIG) XREF_BIBR, XREF_BIBR."

23453991

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sparser

"Hence, VCIP135 could bind to the p97/p47 complex in cytosol, possibly forming an unstable complex of p47, p97, and VCIP135 that was captured after chemical cross-linking."

12473691

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reach

"However, it has also been shown that ATP is required for the p97, p47, and VCIP135 complex to dissociate from Syn5 [XREF_BIBR]."

28819009

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reach

"Other studies have confirmed that the p97, p47, and VCIP135 complex is required for cell-cycle-dependent reformation of the ER network and that p97-p47-mediated reassembly of Golgi cisternae requires the deubiquitinating activity of VCIP135 [XREF_BIBR, XREF_BIBR]."

18687060

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

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sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

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sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( Zhang and Wang, 2015; Zhang et al., 2014 ), and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

27404360

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sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

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sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

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sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

STX5 binds VCP and VCPIP1. 2 / 2

| 1 1

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sparser

"VCIP135 did not bind to syntaxin5 with p97 ( xref C, lane 4), a finding which was investigated further ( xref F)."

12473691

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reach

"Since the p97, VCIP135, and syntaxin5 complex is very unstable (XREF_FIG C), a tighter VCIP135 and p97 complex then dissociates from syntaxin5 (XREF_FIG F)."

12473691

VCP binds VCPIP1 and UBX-L domain. 1 / 1

| 1

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"The DUB VCIP135, central to the regulation of membrane fusion, also binds p97 via a UBX-L domain [XREF_BIBR]."

28819009

VCP binds phosphorylated VCPIP1. 1 / 1

| 1

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reach

"We therefore investigated the effect of phosphorylation on the binding of VCIP135 to p97 or WAC.We first tested whether phosphorylated VCIP135 binds to p97."

23500464

VCP binds VCPIP1 and Ubx. 1 / 1

| 1

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sparser

"In late mitosis, the DUB VCIP135, which associates with p97 via its UBX-L (UBX-like) domain, deubiquitinates Syn5, which permits interaction between the SNAREs, membrane fusion and finally the formation of Golgi cisternae [ xref ]."

28819009

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

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reach

"However, the VCIP135, p97, p47, GST, and syn5DeltaTM complex was dissociated only in the presence of ATP (XREF_FIG E, lane 4), and the VCIP135 and p97 complex was observed in the supernatant (unpublished data)."

12473691

GST binds VCP and VCPIP1. 1 / 1

| 1

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sparser

"Much less VCIP135(T760E, S767E) bound to GST-p97 ( Fig. 2 B, upper panel, lane 2) compared with VCIP135wt (lane 1)."

23500464

VCP binds WAC and VCPIP1. 1 / 1

| 1

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sparser

"We therefore investigated the effect of phosphorylation on the binding of VCIP135 to p97 or WAC."

23500464

VCPIP1 activates VCP.

| 4

VCPIP1 activates VCP. 4 / 4

| 4

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reach

"Next, we wanted to ascertain whether VCIP135 contributes to p97 and p47 mediated membrane fusion using an in vitro Golgi reassembly assay."

12473691

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reach

"The deubiquitinase VCIP135, which is associated with the p97 and p47 complex, removes the ubiquitin moiety and thus allows p97 and p47 to fuse the membranes after mitosis (XREF_FIG) 37."

23453991

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reach

"Considering that the disassembly of the p97, p47, and SNARE complex by VCIP135 requires ATP hydrolysis (XREF_FIG E), we suggest that the function of VCIP135 in membrane fusion is to mediate the dissociation of the p97, p47, and SNARE complex, which may be part of the SNARE priming process."

12473691

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reach

"As shown in XREF_FIG G, VCIP135 partially prevented alpha-SNAP binding to GST-syn5DeltaTM, suggesting that VCIP135 directs syntaxin5 to p97 and p47 but not to alpha-SNAP and NSF."

12473691

VCPIP1 inhibits VCP.

| 3

VCPIP1 inhibits VCP. 3 / 3

| 3

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reach

"During mitosis, Cdk1 phosphorylates p47, p37 and VCIP135, and blocks p97 controlled membrane-fusion processes so that the Golgi membranes remain disassembled."

26734607

➶

reach

"Our in vitro Golgi reassembly assay confirmed that VCIP135 phosphorylation at S130 attenuates p97 and p47 mediated Golgi membrane fusion (XREF_FIG)."

25904330

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reach

"In the presence of ATP, the addition of VCIP135 prevented p97 and p47 from binding to syntaxin5 and VCIP135 itself only bound poorly to syntaxin5 (XREF_FIG D, left three lanes)."

12473691

CDK1 affects VCPIP1

1 2 | 13 18 8

CDK1 phosphorylates VCPIP1. 10 / 19

| 5 14

➶

reach

"During mitosis, Cdk1 phosphorylates p47, p37 and VCIP135, and blocks p97 controlled membrane-fusion processes so that the Golgi membranes remain disassembled."

26734607

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reach

"VCIP135 phosphorylated by Cdc2 generated almost the same amount of mono ubiquitin after the reaction as non phosphorylated VCIP135."

23500464

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sparser

"VCIP135 phosphorylated by Cdc2 generated almost the same amount of mono ubiquitin after the reaction as non-phosphorylated VCIP135 ( Fig. 3 C, top panel, lane 2)."

23500464

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sparser

31435807

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reach

"All these results indicate that phosphorylation of Threonine 760 and Serine 767 in VCIP135 inhibits its binding to p97.We next tested the binding of phosphorylated VCIP135 to WAC, as presented in Fig.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23500464

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sparser

31435807

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reach

"To confirm this, we investigated whether the purified Cdc2 complex phosphorylated VCIP135 in the absence of mitotic cytosol."

23500464

➶

sparser

"These results suggest that VCIP135 is phosphorylated on S130 by the mitotic kinase Cdk1."

25904330

➶

sparser

"To confirm this, we investigated whether the purified Cdc2 complex phosphorylated VCIP135 in the absence of mitotic cytosol."

23500464

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reach

"As shown in Fig. 1 C, Cdc2 indeed phosphorylated VCIP135 (right lane)."

23500464

CDK1 phosphorylates VCPIP1 on S131. 10 / 15

1 | 4 7

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reach

"Cdk1 phosphorylates VCIP135 at S130."

25904330

➶

rlimsp

"To determine whether Cdk1 directly phosphorylates VCIP135 on S130, we performed an in vitro phosphorylation assay with streptavidin-binding peptide (SBP)-tagged VCIP135 recombinant proteins."

25904330

➶

rlimsp

"Therefore Cdk1 directly phosphorylates VCIP135 on S130 in mitosis."

25904330

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reach

"To determine whether Cdk1 directly phosphorylates VCIP135 on S130, we performed an in vitro phosphorylation assay with streptavidin binding peptide (SBP)-tagged VCIP135 recombinant proteins."

25904330

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reach

"Therefore Cdk1 directly phosphorylates VCIP135 on S130 in mitosis."

25904330

➶

rlimsp

"Note that Cdk1 directly phosphorylates VCIP135 on S130."

25904330

➶

biopax:reactome

No evidence text available

➶

reach

"These results suggest that VCIP135 is phosphorylated on S130 by the mitotic kinase Cdk1."

25904330

➶

rlimsp

"We show that, in early mitosis, phosphorylation of VCIP135 by Cdk1 at a single residue, S130, is sufficient to inactivate the enzyme and inhibit p97/p47-mediated Golgi membrane fusion."

25904330

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rlimsp

"Cdk1 phosphorylates VCIP135 at S130."

25904330

CDK1 phosphorylates VCPIP1 on S768. 6 / 7

1 | 2 2 1

➶

rlimsp

"We clarified that VCIP135, an essential factor in both p97 membrane fusion pathways, is phosphorylated on Threonine-760 and Serine-767 by Cdc2 at mitosis and that this phosphorylated VCIP135 does not bind to p97."

23500464

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reach

"We show that VCIP135 is phosphorylated on Threonine 760 and Serine 767 by Cdc2 at mitosis."

23500464

➶

sparser

"Taken together, we conclude that VCIP135 is mitotically phosphorylated on Threonine-760 and Serine-767 by Cdc2."

23500464

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sparser

"We show that VCIP135 is phosphorylated on Threonine-760 and Serine-767 by Cdc2 at mitosis."

23500464

➶

biopax:phosphositeplus

No evidence text available

➶

reach

"We clarified that VCIP135, an essential factor in both p97 membrane fusion pathways, is phosphorylated on Threonine 760 and Serine 767 by Cdc2 at mitosis and that this phosphorylated VCIP135 does not bind to p97."

23500464

CDK1 phosphorylates VCPIP1 on T761. 5 / 6

1 | 2 2

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sparser

"We show that VCIP135 is phosphorylated on Threonine-760 and Serine-767 by Cdc2 at mitosis."

23500464

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reach

"We show that VCIP135 is phosphorylated on Threonine 760 and Serine 767 by Cdc2 at mitosis."

23500464

➶

sparser

"Taken together, we conclude that VCIP135 is mitotically phosphorylated on Threonine-760 and Serine-767 by Cdc2."

23500464

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biopax:phosphositeplus

No evidence text available

➶

reach

23500464

VCPIP1 affects STX5

2 2 | 12 15

VCPIP1 binds STX5.

2 2 | 8 15

STX5 binds VCPIP1. 10 / 30

2 1 | 7 12

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sparser

"VCIP135 bound to syntaxin5 is thought to associate with a syntaxin5-bound p97/p47 complex with syntaxin5 acting as a scaffold for the p97/p47 dissociation reaction."

12473691

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reach

"The binding of VCIP135 to syntaxin5 independently of p47 (XREF_FIG C) allows VCIP135 to be closely associated with the p97 and p47 complex, whereas in cytosol such an association is less likely."

12473691

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sparser

"The specificity of binding of VCIP135 to syntaxin5 was further confirmed by the coimmunoprecipitation experiments using Golgi membranes as shown in xref B."

12473691

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sparser

"These data suggest that both VCIP135 and the p97/p47 complex bind to syntaxin5, resulting in the formation of a transient quaternary complex that could be dissociated through p97 ATP hydrolysis."

12473691

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reach

"These data suggest that both VCIP135 and the p97 and p47 complex bind to syntaxin5, resulting in the formation of a transient quaternary complex that could be dissociated through p97 ATP hydrolysis."

12473691

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sparser

"Although VCIP135 can bind to p97 in cytosol ( xref A), VCIP135 bound to syntaxin5 does not form a complex with p97 ( xref C, lane 4)."

12473691

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sparser

"VCIP135 forms a quaternary complex with syntaxin 5 and the p97/p47 complex via a ubiquitin fold domain and its enzymatic activity is required for post-mitotic Golgi membrane fusion xref ."

23453991

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sparser

"However, in the presence of AMP–PNP or ADP, p97 and p47 bound to syntaxin5 even when VCIP135 was added, and VCIP135 also bound to syntaxin5 ( xref D, right six lanes)."

12473691

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sparser

"We have shown that VCIP135 binds to syntaxin5 and disassembles the p97/p47/syntaxin5 complex through p97 ATP hydrolysis."

12473691

➶

biogrid

No evidence text available

12473691

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

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sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

STX5 binds VCP and VCPIP1. 2 / 2

| 1 1

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sparser

"VCIP135 did not bind to syntaxin5 with p97 ( xref C, lane 4), a finding which was investigated further ( xref F)."

12473691

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reach

"Since the p97, VCIP135, and syntaxin5 complex is very unstable (XREF_FIG C), a tighter VCIP135 and p97 complex then dissociates from syntaxin5 (XREF_FIG F)."

12473691

VCPIP1 deubiquitinates STX5.

| 2

VCPIP1 deubiquitinates STX5. 2 / 2

| 2

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reach

"Syntaxin 5 is monoubiquitinated by HACE1 in early mitosis and deubiquitinated by the de-ubiquitinase VCIP135 in late mitosis (Wang et al. 2004)."

31435807

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reach

"Syntaxin 5 is monoubiquitinated by HACE1 in early mitosis and deubiquitinated by the de-ubiquitinase VCIP135 in late mitosis."

31435807

VCPIP1 activates STX5.

| 2

VCPIP1 activates STX5. 2 / 2

| 2

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reach

"As shown in XREF_FIG G, VCIP135 partially prevented alpha-SNAP binding to GST-syn5DeltaTM, suggesting that VCIP135 directs syntaxin5 to p97 and p47 but not to alpha-SNAP and NSF."

12473691

➶

reach

31435807

VCPIP1 affects SPRTN

1 | 1 23 4

VCPIP1 deubiquitinates SPRTN.

| 17

VCPIP1 deubiquitinates SPRTN. 10 / 17

| 17

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reach

"These observations show that depletion of USP11 and VCPIP1 inhibits SPRTN deubiquitination, but in contrast to previous reports, monoubiquitination does not restrict SPRTN access to chromatin."

33567341

➶

reach

"Upon DPC induction, ATM and ATR kinase activates VCPIP1 and VCIP135 deubiquitinase, which in turn deubiquitinates SPRTN, regulating its chromatin localization."

33567341

➶

reach

"VCPIP1, in turn, deubiquitinates SPRTN and promotes its chromatin relocalization."

32649882

➶

reach

"These results suggest that VCPIP1 deubiquitinates SPRTN both in vitro and in cells."

32649882

➶

reach

"We hypothesized that VCPIP1 deubiquitinates SPRTN and promotes SPRTN-mediated DPC repair."

32649882

➶

reach

"We found that knockdown of VCPIP1 decreased acetylation of SPRTN upon DPC induction (Figure 4I), suggesting that SPRTN acetylation requires SPRTN deubiquitination."

32649882

➶

reach

"We observed reduced SPRTN auto-cleavage upon DPC induction in USP11 and USP7 single and double-knockdown cells, suggesting that in the absence of USP11 and USP7, SPRTN could be deubiquitinated by VCPIP1, which is recruited to chromatin upon DPC induction."

33567341

➶

reach

"We found that recombinant VCPIP1-WT, but not VCPIP1-CA, deubiquitinated SPRTN in vitro (Figure 2D)."

32649882

➶

reach

"Our results so far suggest that VCPIP1 deubiquitinates SPRTN, thereby triggering association of SPRTN with chromatin following DPC damage."

32649882

➶

reach

"Of note, while this study was under consideration, it was proposed that SPRTN is deubiquitylated by VCPIP1."

33348378

VCPIP1 binds SPRTN.

1 | 5 4

SPRTN binds VCPIP1. 10 / 10

1 | 5 4

➶

reach

"Consistent with the interaction shown by co-immunoprecipitation, direct association between VCPIP1 and SPRTN in cells was confirmed by proximity ligation assay (PLA) and PLA foci signals were mostly in the nucleus and significantly increased after FA treatment (Figures 1D–1E)."

32649882

➶

reach

"Knockdown of VCP did not change the SPRTN/VCPIP1 interaction (Figure S2J)."

32649882

➶

reach

"We confirmed the interaction between VCPIP1 and SPRTN using co-immunoprecipitation experiments (Figures 1A–1B and S1B–S1C)."

32649882

➶

sparser

"We confirmed the interaction between VCPIP1 and SPRTN using co-immunoprecipitation experiments ( xref – xref and xref – xref )."

32649882

➶

sparser

"Moreover, after treating cells with three known DPC-inducing agents, FA, camptothecin (CPT) and cisplatin ( xref ), the interaction between VCPIP1 and SPRTN increased ( xref and xref – xref )."

32649882

➶

biogrid

No evidence text available

22902628

➶

sparser

"Our data strongly suggest that VCPIP1-SPRTN signaling axis is the key regulation in DPC repair."

32649882

➶

sparser

"As VCPIP1-SPRTN signaling protects cells from DPC induction, it seems likely that inhibition of VCPIP1-SPRTN could be employed to sensitize cells to different kinds of DPC-inducing agents."

32649882

➶

reach

"Moreover, after treating cells with three known DPC-inducing agents, FA, camptothecin (CPT) and cisplatin (Stingele et al., 2017), the interaction between VCPIP1 and SPRTN increased (Figures 1C and S1D–S1E)."

32649882

➶

reach

"VCPIP1 and USP11 interact weakly with SPRTN in co-immunoprecipitating experiments, but show no (VCPIP1) or only weak (USP11) preference for SPRTN-Ub (XREF_SUPPLEMENTARY)."

33348378

VCPIP1 activates SPRTN.

| 1

VCPIP1 activates SPRTN. 1 / 1

| 1

➶

eidos

"Knockdown of VCPIP1 greatly reduced the interaction between SPRTN and PCAF / GCN5 in the presence of DPCs ( Figure S4H ) , suggesting that VCPIP1 is required to bring acetyltransferases to SPRTN in a DPC-dependent manner ."

32649882

VCPIP1 acetylates SPRTN.

| 1

VCPIP1 leads to the acetylation of SPRTN. 1 / 1

| 1

➶

reach

"We found that knockdown of VCPIP1 decreased acetylation of SPRTN upon DPC induction (Figure 4I), suggesting that SPRTN acetylation requires SPRTN deubiquitination."

32649882

VCPIP1 affects NSFL1C

4 1 | 13 10

VCPIP1 binds NSFL1C.

4 1 | 8 10

NSFL1C binds VCPIP1. 10 / 12

4 | 4 4

➶

sparser

"The inhibition of p97 ATPase activity can be reduced if both p47-binding sites are not occupied, and the metastable complex of VCIP135/p97/p47 would represent such a case: VCIP135 interacts with the p97/p47 complex that uses only one p47-binding site."

12473691

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sparser

"Several lines of recent evidence suggest that ubiquitination may be implicated in the p97/p47 pathway; p47 binds to monoubiquitinated proteins and VCIP135 shows a deubiquitinating activity in vitro."

15749824

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sparser

"VCIP135 cannot interact with p47 directly ( xref A)."

12473691

➶

reach

"The p97 and p47 binding protein VCIP135 (VCP [p97] and p47 complex interacting protein, p135) functions as a deubiquitinating enzyme whose activity is required for post-mitotic Golgi reassembly 26."

29225785

➶

biogrid

No evidence text available

18775313

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biogrid

No evidence text available

19615732

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reach

"We have shown that VCIP135 binds to syntaxin5 and disassembles the p97, p47, and syntaxin5 complex through p97 ATP hydrolysis."

12473691

➶

reach

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97, p47, and Syn5 complex."

28819009

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sparser

"As ATPases, NSF and p97 have specific co-factors: NSF-mediated fusion requires the proteins SNAPs and p115, while the p97-mediated pathway requires formation of a complex with p47 and VCIP135, or with p37 (Kondo et al., xref ; Uchiyama et al., xref )."

26734607

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biogrid

No evidence text available

12473691

VCP binds NSFL1C and VCPIP1. 4 / 4

| 3 1

➶

reach

23453991

➶

sparser

"Hence, VCIP135 could bind to the p97/p47 complex in cytosol, possibly forming an unstable complex of p47, p97, and VCIP135 that was captured after chemical cross-linking."

12473691

➶

reach

"However, it has also been shown that ATP is required for the p97, p47, and VCIP135 complex to dissociate from Syn5 [XREF_BIBR]."

28819009

➶

reach

18687060

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

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sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

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sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

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sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

VCPIP1 activates NSFL1C.

| 3

VCPIP1 activates NSFL1C. 3 / 3

| 3

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reach

"The deubiquitinase VCIP135, which is associated with the p97 and p47 complex, removes the ubiquitin moiety and thus allows p97 and p47 to fuse the membranes after mitosis (XREF_FIG) 37."

23453991

➶

reach

"Next, we wanted to ascertain whether VCIP135 contributes to p97 and p47 mediated membrane fusion using an in vitro Golgi reassembly assay."

12473691

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reach

"As shown in XREF_FIG G, VCIP135 partially prevented alpha-SNAP binding to GST-syn5DeltaTM, suggesting that VCIP135 directs syntaxin5 to p97 and p47 but not to alpha-SNAP and NSF."

12473691

VCPIP1 inhibits NSFL1C.

| 2

VCPIP1 inhibits NSFL1C. 2 / 2

| 2

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reach

"Our in vitro Golgi reassembly assay confirmed that VCIP135 phosphorylation at S130 attenuates p97 and p47 mediated Golgi membrane fusion (XREF_FIG)."

25904330

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reach

"In the presence of ATP, the addition of VCIP135 prevented p97 and p47 from binding to syntaxin5 and VCIP135 itself only bound poorly to syntaxin5 (XREF_FIG D, left three lanes)."

12473691

NSFL1C affects VCPIP1

4 1 | 9 10

NSFL1C binds VCPIP1.

4 1 | 8 10

NSFL1C binds VCPIP1. 10 / 12

4 | 4 4

➶

sparser

12473691

➶

sparser

15749824

➶

sparser

"VCIP135 cannot interact with p47 directly ( xref A)."

12473691

➶

reach

"The p97 and p47 binding protein VCIP135 (VCP [p97] and p47 complex interacting protein, p135) functions as a deubiquitinating enzyme whose activity is required for post-mitotic Golgi reassembly 26."

29225785

➶

biogrid

No evidence text available

18775313

➶

biogrid

No evidence text available

19615732

➶

reach

"We have shown that VCIP135 binds to syntaxin5 and disassembles the p97, p47, and syntaxin5 complex through p97 ATP hydrolysis."

12473691

➶

reach

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97, p47, and Syn5 complex."

28819009

➶

sparser

26734607

➶

biogrid

No evidence text available

12473691

VCP binds NSFL1C and VCPIP1. 4 / 4

| 3 1

➶

reach

23453991

➶

sparser

"Hence, VCIP135 could bind to the p97/p47 complex in cytosol, possibly forming an unstable complex of p47, p97, and VCIP135 that was captured after chemical cross-linking."

12473691

➶

reach

"However, it has also been shown that ATP is required for the p97, p47, and VCIP135 complex to dissociate from Syn5 [XREF_BIBR]."

28819009

➶

reach

18687060

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

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sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

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reach

12473691

NSFL1C activates VCPIP1.

| 1

NSFL1C activates VCPIP1. 1 / 1

| 1

➶

reach

"WAC is hence thought to function in p97 and p47 mediated Golgi membrane fusion by activating the deubiquitinating function of VCIP135."

21811234

WAC affects VCPIP1

4 | 1 6 7

WAC binds VCPIP1. 10 / 15

4 | 1 4 5

➶

sparser

"WAC directly binds to VCIP135 and increases its deubiquitinating activity."

21811234

➶

biogrid

No evidence text available

21811234

➶

biogrid

No evidence text available

21811234

➶

reach

"WAC directly binds to VCIP135 and increases its deubiquitinating activity."

21811234

➶

sparser

"In this study, we isolated a VCIP135 mutant, VCIP135(T760E, S767E), that still binds to WAC and maintains its deubiquitinating enzyme activity, but lacks p97-binding affinity."

23500464

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sparser

"Furthermore, VCIP135 interacts with WAC, which enhances the DUB activity of VCIP135."

23453991

➶

reach

"Golgi WAC interacts with the deubiquitinase VCIP135, which binds VCP and p97."

26687599

➶

biogrid

No evidence text available

21811234

➶

sparser

"WAC binds to VCIP135, mediating its association with the Golgi, and activating its deubiquitinating function to regulate Golgi biogenesis ( xref )."

26812014

➶

biogrid

No evidence text available

23500464

WAC binds VCPIP1-S767E. 1 / 1

| 1

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reach

"In this study, we isolated a VCIP135 mutant, VCIP135 (T760E, S767E), that still binds to WAC and maintains its deubiquitinating enzyme activity, but lacks p97 binding affinity."

23500464

GST binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

"We next tested the binding of phosphorylated VCIP135 to WAC, as presented in Fig. 3 A. Cdc2-phosphorylated VCIP135 still bound to GST-WAC (upper panel, lane 2), suggesting that the phosphorylation of[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23500464

VCP binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

"We therefore investigated the effect of phosphorylation on the binding of VCIP135 to p97 or WAC."

23500464

WAC binds VCPIP1-T760E. 1 / 1

| 1

➶

reach

"In this study, we isolated a VCIP135 mutant, VCIP135 (T760E, S767E), that still binds to WAC and maintains its deubiquitinating enzyme activity, but lacks p97 binding affinity."

23500464

VCPIP1 affects SNTG2

1 | 12 3

VCPIP1 deubiquitinates SNTG2.

1 | 8

VCPIP1 deubiquitinates SNTG2. 9 / 9

1 | 8

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reach

"One possible explanation, as described in our model (XREF_FIG), is that monoubiquitination of Syn5 occurs only transiently in early mitosis and is quickly reversed by the deubiquitinase VCIP135 in late mitosis."

27404360

➶

reach

28819009

➶

reach

"Therefore, we determined whether Syn5 is deubiquitinated by VCIP135."

27404360

➶

reach

"Taken together, these results demonstrate that Syn5 is deubiquitinated by VCIP135 in late mitosis.Syn5 contains 17 lysines that are conserved between rat and human."

27404360

➶

reach

"One possible explanation, as described in our model, is that monoubiquitination of Syn5 occurs only transiently in early mitosis and is quickly reversed by the deubiquitinase VCIP135 in late mitosis."

27404360

➶

reach

"Syn5 is monoubiquitinated by the ubiquitin ligase HACE1 in early mitosis and deubiquitinated by the deubiquitinase VCIP135 in late mitosis."

27404360

➶

reach

"These results suggest that Syn5 is deubiquitinated by VCIP135."

27404360

➶

ubibrowser

"Syn5 is monoubiquitinated by the ubiquitin ligase HACE1 in early mitosis and deubiquitinated by the deubiquitinase VCIP135 in late mitosis."

27404360

➶

reach

"Taken together, these results demonstrate that Syn5 is deubiquitinated by VCIP135 in late mitosis."

27404360

VCPIP1 activates SNTG2.

| 4

VCPIP1 activates SNTG2. 4 / 4

| 4

➶

reach

"To investigate whether ubiquitinated Syn5 is a direct substrate of VCIP135, in vitro ubiquitinated Syn5 was treated with recombinant VCIP135."

27404360

➶

reach

"To investigate whether ubiquitinated Syn5 is a direct substrate of VCIP135, in vitro ubiquitinated Syn5 was treated with recombinant VCIP135 (Zhang and Wang, 2015; Zhang et al., 2014)."

27404360

➶

reach

"In late mitosis, VCIP135 is reactivated by dephosphorylation, removes ubiquitin from Syn5, and allows Syn5 interacting with its cognate SNARE Bet1 for membrane fusion at mitotic exit (XREF_FIG, right panel)."

27404360

➶

reach

"In late mitosis, VCIP135 is reactivated by dephosphorylation, removes ubiquitin from Syn5, and allows Syn5 to interact with its cognate SNARE Bet1 for membrane fusion at mitotic exit."

27404360

VCPIP1 binds SNTG2.

| 3

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

VCP affects NSFL1C

1 | 4 6

VCP binds NSFL1C and VCPIP1. 4 / 4

| 3 1

➶

reach

23453991

➶

sparser

"Hence, VCIP135 could bind to the p97/p47 complex in cytosol, possibly forming an unstable complex of p47, p97, and VCIP135 that was captured after chemical cross-linking."

12473691

➶

reach

"However, it has also been shown that ATP is required for the p97, p47, and VCIP135 complex to dissociate from Syn5 [XREF_BIBR]."

28819009

➶

reach

18687060

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

➶

sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

SPRTN affects VCPIP1

1 | 6 4

SPRTN binds VCPIP1.

1 | 5 4

SPRTN binds VCPIP1. 10 / 10

1 | 5 4

➶

reach

32649882

➶

reach

"Knockdown of VCP did not change the SPRTN/VCPIP1 interaction (Figure S2J)."

32649882

➶

reach

"We confirmed the interaction between VCPIP1 and SPRTN using co-immunoprecipitation experiments (Figures 1A–1B and S1B–S1C)."

32649882

➶

sparser

"We confirmed the interaction between VCPIP1 and SPRTN using co-immunoprecipitation experiments ( xref – xref and xref – xref )."

32649882

➶

sparser

"Moreover, after treating cells with three known DPC-inducing agents, FA, camptothecin (CPT) and cisplatin ( xref ), the interaction between VCPIP1 and SPRTN increased ( xref and xref – xref )."

32649882

➶

biogrid

No evidence text available

22902628

➶

sparser

"Our data strongly suggest that VCPIP1-SPRTN signaling axis is the key regulation in DPC repair."

32649882

➶

sparser

"As VCPIP1-SPRTN signaling protects cells from DPC induction, it seems likely that inhibition of VCPIP1-SPRTN could be employed to sensitize cells to different kinds of DPC-inducing agents."

32649882

➶

reach

32649882

➶

reach

"VCPIP1 and USP11 interact weakly with SPRTN in co-immunoprecipitating experiments, but show no (VCPIP1) or only weak (USP11) preference for SPRTN-Ub (XREF_SUPPLEMENTARY)."

33348378

SPRTN activates VCPIP1.

| 1

SPRTN activates VCPIP1. 1 / 1

| 1

➶

reach

"Upon DPC induction, the deubiquitinase VCPIP1/VCIP135 is activated by ATM/ATR, which then deubiquitinates SPRTN."

32649882

NSFL1C affects VCP

1 | 4 6

VCP binds NSFL1C and VCPIP1. 4 / 4

| 3 1

➶

reach

23453991

➶

sparser

"Hence, VCIP135 could bind to the p97/p47 complex in cytosol, possibly forming an unstable complex of p47, p97, and VCIP135 that was captured after chemical cross-linking."

12473691

➶

reach

"However, it has also been shown that ATP is required for the p97, p47, and VCIP135 complex to dissociate from Syn5 [XREF_BIBR]."

28819009

➶

reach

18687060

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

➶

sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

Pol affects VCPIP1

| 5

Pol phosphorylates VCPIP1. 5 / 5

| 5

➶

sparser

"These results demonstrate that VCIP135 S130 phosphorylation regulates its DUB activity."

25904330

➶

sparser

"Because S130 matches the minimal S/T-P consensus motif for both Cdk1 ( xref ) and ERK1/2 ( xref ; xref ), we applied inhibitors to these protein kinases to mitotic cells and investigated the effects on S130 phosphorylation of VCIP135."

25904330

➶

sparser

"We next determined the kinase responsible for VCIP135 S130 phosphorylation in mitosis."

25904330

➶

sparser

"VCIP135 S130 phosphorylation in the cell cycle."

25904330

➶

sparser

"Because VCIP135 DUB activity is specifically required for postmitotic Golgi membrane fusion, we determined the effects of S130 phosphorylation of VCIP135 on postmitotic Golgi reassembly in vivo."

25904330

VCP affects STX5

1 | 1 3

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

➶

sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

STX5 binds VCP and VCPIP1. 2 / 2

| 1 1

➶

sparser

"VCIP135 did not bind to syntaxin5 with p97 ( xref C, lane 4), a finding which was investigated further ( xref F)."

12473691

➶

reach

"Since the p97, VCIP135, and syntaxin5 complex is very unstable (XREF_FIG C), a tighter VCIP135 and p97 complex then dissociates from syntaxin5 (XREF_FIG F)."

12473691

ATR affects VCPIP1

| 5

ATR phosphorylates VCPIP1. 5 / 5

| 5

➶

reach

"Taken together, these data suggest that VCPIP1 phosphorylation by ATM/ATR is important for VCPIP1 activation following DPC induction."

32649882

➶

reach

"These results suggest that VCPIP1 is phosphorylated by ATM/ATR following DPC induction."

32649882

➶

reach

"Here we report that, in response to DPC induction, the deubiquitinase VCPIP1 and VCIP135 is phosphorylated and activated by ATM and ATR."

32649882

➶

reach

"VCPIP1 is phosphorylated by ATM/ATR upon DPC induction."

32649882

➶

reach

"Here, we report that in response to DPC induction, the deubiquitinase VCPIP1/VCIP135 is phosphorylated and activated by ATM/ATR."

32649882

ATM affects VCPIP1

| 5

ATM phosphorylates VCPIP1. 5 / 5

| 5

➶

reach

"Here we report that, in response to DPC induction, the deubiquitinase VCPIP1 and VCIP135 is phosphorylated and activated by ATM and ATR."

32649882

➶

reach

"Here, we report that in response to DPC induction, the deubiquitinase VCPIP1/VCIP135 is phosphorylated and activated by ATM/ATR."

32649882

➶

reach

"VCPIP1 is phosphorylated by ATM/ATR upon DPC induction."

32649882

➶

reach

"These results suggest that VCPIP1 is phosphorylated by ATM/ATR following DPC induction."

32649882

➶

reach

"Taken together, these data suggest that VCPIP1 phosphorylation by ATM/ATR is important for VCPIP1 activation following DPC induction."

32649882

VCPIP1 affects MYBL1

| 4

VCPIP1 binds MYBL1. 4 / 4

| 4

➶

sparser

"RNA-Seq identified MYBL1-NFIB , NFIB-TBPL1 , and MYBL1-VCPIP1 fusion transcripts, respectively, supporting the diagnosis of AdCC in these three tumors."

34176176

➶

sparser

"Adenoid cystic carcinoma is characterized by a recurrent t(6;9)(q22–23;p23–24) translocation that results in a MYB-NFIB fusion gene. xref , xref An alternative MYBL1-NFIB gene fusion has been reported in a subset of cases. xref , xref , xref Various other genes have been reported to substitute for MYB , MYBL1 and NFIB , including TGFRB3, RAD51B, YTHDF3, AIG1, XRCC4 and PTPRD , amongst others. xref , xref , xref – xref In our cohort of 20 AdCCs, the MYB-NFIB fusion was identified in 60% and the MYBL1-NFIB fusion in 25%, while two cases harbored novel NFIB-TBPL1 and MYBL1-VCPIP1 fusions products."

34176176

➶

sparser

"MYB was rearranged in 60% (12/20), MYBL1 in 30% (6/20) and NFIB in 5% (1/20); two tumors exhibited novel fusion products, including NFIB-TBPL1 and MYBL1-VCPIP1 ."

34176176

➶

sparser

"In addition, two cases were found to harbor novel fusion genes ( NFIB-TBPL1 and MYBL1-VCPIP1 )."

34176176

NSFL1C affects VCP, and VCPIP1

| 3 1

VCP binds NSFL1C and VCPIP1. 4 / 4

| 3 1

➶

reach

23453991

➶

sparser

"Hence, VCIP135 could bind to the p97/p47 complex in cytosol, possibly forming an unstable complex of p47, p97, and VCIP135 that was captured after chemical cross-linking."

12473691

➶

reach

"However, it has also been shown that ATP is required for the p97, p47, and VCIP135 complex to dissociate from Syn5 [XREF_BIBR]."

28819009

➶

reach

18687060

MYBL1 affects VCPIP1

| 4

VCPIP1 binds MYBL1. 4 / 4

| 4

➶

sparser

"RNA-Seq identified MYBL1-NFIB , NFIB-TBPL1 , and MYBL1-VCPIP1 fusion transcripts, respectively, supporting the diagnosis of AdCC in these three tumors."

34176176

➶

sparser

34176176

➶

sparser

"MYB was rearranged in 60% (12/20), MYBL1 in 30% (6/20) and NFIB in 5% (1/20); two tumors exhibited novel fusion products, including NFIB-TBPL1 and MYBL1-VCPIP1 ."

34176176

➶

sparser

"In addition, two cases were found to harbor novel fusion genes ( NFIB-TBPL1 and MYBL1-VCPIP1 )."

34176176

Bisphenol A affects VCPIP1

3 |

Bisphenol A increases the amount of VCPIP1. 3 / 3

3 |

➶

ctd

No evidence text available

25181051

➶

ctd

No evidence text available

30816183

➶

ctd

No evidence text available

29097150

VCPIP1

| 3

VCPIP1 translocates to the membrane. 3 / 3

| 3

➶

sparser

"Subsequently, the p97/p47 complex recruits VCIP135 to the membrane."

21988917

➶

sparser

"Monoubiquitination of Syn5 recruits p97/p47 and VCIP135 to Golgi membranes through the interaction with the UBA domain of p47 ( xref , middle panel)."

27404360

➶

sparser

"Monoubiquitination of Syn5 recruits p97/p47 and VCIP135 to Golgi membranes through the interaction with the UBA domain of p47 ( Figure 7 F, middle panel)."

27404360

VCPIP1 affects sub

| 3

VCPIP1 inhibits sub.

| 2

VCPIP1 inhibits sub. 2 / 2

| 2

➶

reach

"In this study, we found that phosphorylation of VCIP135 at a single site, S130, is necessary and sufficient to inactivate its DUB activity for Golgi disassembly in early mitosis."

25904330

➶

reach

25904330

VCPIP1 activates sub.

| 1

VCPIP1 activates sub. 1 / 1

| 1

➶

reach

"Dephosphorylation of S130 on VCIP135 upon staurosporine or roscovitine but not U0126 treatment also significantly increased the DUB activity of endogenous VCIP135 (XREF_FIG), consistent with the hypothesis that phosphorylation at S130 inactivates VCIP135."

25904330

VCPIP1 affects X

| 2 1

VCPIP1 binds X.

| 1 1

VCPIP1 binds X. 2 / 2

| 1 1

➶

reach

"Functionally, HBx specifically binding to VCPIP1 significantly enhanced the transcriptional transactivation of HBx by activating NF-κB, AP-1, and SP-1 and inhibited hepatoma cell clonogenicity in Huh7 and HepG2 cells."

35695579

➶

sparser

35695579

VCPIP1 increases the amount of X.

| 1

VCPIP1 increases the amount of X. 1 / 1

| 1

➶

reach

"VCPIP1 and its C-terminal amino acids 863 to 1221 upregulated the HBx protein expression, with or without HBV infection."

35695579

VCPIP1 affects Top1cc

| 3

VCPIP1 inhibits Top1cc.

| 2

VCPIP1 inhibits Top1cc. 2 / 2

| 2

➶

reach

"Loss of VCPIP1 resulted in cellular hypersensitivity to DPC-inducing agents, as well as Top1cc and DPC accumulation, suggesting that VCPIP1 plays a critical role in DPC repair."

32649882

➶

reach

"We found that VCPIP1 deficiency resulted in enhanced Top1cc foci signals after CPT treatment (Figures 1M–1N), suggesting a role of VCPIP1 in Top1cc removal."

32649882

VCPIP1 activates Top1cc.

| 1

VCPIP1 activates Top1cc. 1 / 1

| 1

➶

reach

"In agreement with this data, VCPIP1-WT rather than VCPIP1-CA could promote Top1cc removal by SPRTN (Figures 2G–2H)."

32649882

VCPIP1 affects GST-syn5DeltaTM

| 3

VCPIP1 binds GST-syn5DeltaTM. 3 / 3

| 3

➶

reach

"We found that GST-syn5DeltaTM bound VCIP135 very efficiently (XREF_FIG A, lane 1) compared with GST-syn1DeltaTM (lane 2)."

12473691

➶

reach

"To address this, we first performed binding experiments : p97 and p47 was incubated with either GST-syn5DeltaTM or VCIP135 + GST-syn5DeltaTM in the presence of nucleotides, and the proteins bound to GST-syn5DeltaTM were analyzed (XREF_FIG D; see also Fig."

12473691

➶

reach

"VCIP135 bound to GST-syn5DeltaTM either with p47 or p97 and p47 (XREF_FIG C, lanes 5 and 6, respectively)."

12473691

VCPIP1 affects GST

| 1 2

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

GST binds VCP and VCPIP1. 1 / 1

| 1

➶

sparser

"Much less VCIP135(T760E, S767E) bound to GST-p97 ( Fig. 2 B, upper panel, lane 2) compared with VCIP135wt (lane 1)."

23500464

GST binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

23500464

VCP affects SNTG2

| 3

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

STX5 affects NSFL1C

1 | 2

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

➶

sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

SNTG2 affects VCPIP1

| 3

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

NSFL1C affects STX5, VCP, and VCPIP1

1 | 2

STX5 binds VCP, NSFL1C, and VCPIP1. 3 / 3

1 | 2

➶

hprd

No evidence text available

12473691

➶

sparser

"VCIP135 binds to the p97–p47–syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5 ( xref )."

15037600

➶

sparser

"This simple binding experiment showed that VCIP135 can form a quaternary complex with syntaxin5, p97, and p47 (Fig. S2 available at)."

12473691

NSFL1C affects SNTG2, VCP, and VCPIP1

| 3

VCP binds SNTG2, NSFL1C, and VCPIP1. 3 / 3

| 3

➶

sparser

"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97–p47–Syn5 complex."

28819009

➶

sparser

27404360

➶

sparser

"At this time VCIP135 is inactivated by mitotic phosphorylation ( xref ; xref ) and thus monoubiquitinated Syn5, p97, p47, and VCIP135 form a transient quaternary complex ( xref )."

27404360

GST-syn5DeltaTM affects VCPIP1

| 3

VCPIP1 binds GST-syn5DeltaTM. 3 / 3

| 3

➶

reach

"We found that GST-syn5DeltaTM bound VCIP135 very efficiently (XREF_FIG A, lane 1) compared with GST-syn1DeltaTM (lane 2)."

12473691

➶

reach

12473691

➶

reach

"VCIP135 bound to GST-syn5DeltaTM either with p47 or p97 and p47 (XREF_FIG C, lanes 5 and 6, respectively)."

12473691

GST affects VCPIP1

| 1 2

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

GST binds VCP and VCPIP1. 1 / 1

| 1

➶

sparser

"Much less VCIP135(T760E, S767E) bound to GST-p97 ( Fig. 2 B, upper panel, lane 2) compared with VCIP135wt (lane 1)."

23500464

GST binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

23500464

Valproic acid affects VCPIP1

2 |

Valproic acid demethylates VCPIP1.

1 |

Valproic acid demethylates VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

29154799

Valproic acid decreases the amount of VCPIP1.

1 |

Valproic acid decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

23179753

Succimer affects VCPIP1

2 |

Succimer decreases the amount of VCPIP1. 2 / 2

2 |

➶

ctd

No evidence text available

26378955

➶

ctd

No evidence text available

21641980

Magnetite nanoparticle affects VCPIP1

2 |

Magnetite nanoparticle decreases the amount of VCPIP1. 2 / 2

2 |

➶

ctd

No evidence text available

26378955

➶

ctd

No evidence text available

21641980

Aflatoxin B1 affects VCPIP1

2 |

Aflatoxin B1 increases the amount of VCPIP1.

1 |

Aflatoxin B1 increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

19770486

Aflatoxin B1 demethylates VCPIP1.

1 |

Aflatoxin B1 demethylates VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27153756

ZEB1 affects VCPIP1

2 |

ZEB1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

29119051

➶

biogrid

No evidence text available

29119051

X affects VCPIP1

| 1 1

VCPIP1 binds X. 2 / 2

| 1 1

➶

reach

35695579

➶

sparser

35695579

VCPIP1 affects ZEB1

2 |

ZEB1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

29119051

➶

biogrid

No evidence text available

29119051

VCPIP1 affects VCPIP1

| 2

VCPIP1 bound to STX5 activates VCPIP1. 1 / 1

| 1

➶

reach

"The binding of VCIP135 to syntaxin5 independently of p47 (XREF_FIG C) allows VCIP135 to be closely associated with the p97 and p47 complex, whereas in cytosol such an association is less likely."

12473691

VCPIP1 activates VCPIP1. 1 / 1

| 1

➶

reach

25904330

VCPIP1 affects Ubiquitin

| 2

VCPIP1 inhibits Ubiquitin. 2 / 2

| 2

➶

reach

"As shown in Figure 3 A (lanes 3 and 4 versus lane 2), co-expression of WT VCIP135, but not the C218S mutant, significantly reduced the ubiquitin signal."

27404360

➶

reach

"As shown in XREF_FIG (lanes 3 and 4 vs. 2), co-expression of WT VCIP135, but not the C218S mutant, significantly reduced the ubiquitin signal (XREF_FIG)."

27404360

VCPIP1 affects UFD1

2 |

UFD1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

VCPIP1 affects UBXN2B

2 |

UBXN2B binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

VCPIP1 affects UBXN2A

2 |

UBXN2A binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

VCPIP1 affects UBC

2 |

UBC binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

23827681

➶

biogrid

No evidence text available

23500464

VCPIP1 affects TTLL1

2 |

TTLL1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

VCPIP1 affects Qa-SNARE Proteins

| 2

VCPIP1 inhibits Qa-SNARE Proteins. 2 / 2

| 2

➶

eidos

"In mitosis , VCIP135 is phosphorylated at S130 by Cdk1 and thus is inactivated , allowing syntaxin 5 to be ubiquitinated by HACE1 ; in telophase , VCIP135 is dephosphorylated and reactivated , removing ubiquitin from syntaxin 5 to allow p97-mediated membrane fusion Wang 2008 ) ."

31435807

➶

eidos

"In mitosis , VCIP135 is phosphorylated at S130 by Cdk1 and thus is inactivated , allowing syntaxin 5 to be ubiquitinated by HACE1 ; in telophase , VCIP135 is dephosphorylated and reactivated , removing ubiquitin from syntaxin 5 to allow p97-mediated membrane fusion ( Huang and Wang 2017 ; Wang 2008 ) ."

31435807

VCPIP1 affects PRMT2

2 |

VCPIP1 binds PRMT2. 2 / 2

2 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

VCPIP1 affects PLAAT5

2 |

PLAAT5 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

VCPIP1 affects P14357

2 |

VCPIP1 binds P14357. 2 / 2

2 |

➶

virhostnet

No evidence text available

19637933

➶

virhostnet

No evidence text available

19637933

VCPIP1 affects NPLOC4

2 |

NPLOC4 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

VCPIP1 affects MAF1

2 |

MAF1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

VCPIP1 affects Golgi reassembly

| 2

VCPIP1 activates Golgi reassembly. 2 / 2

| 2

➶

reach

"Therefore, monoubiquitinated syntaxin 5 recruits p97/p47 to the mitotic Golgi fragments and promotes post-mitotic Golgi reassembly upon ubiquitin removal by VCIP135 ."

31435807

➶

reach

"Therefore, monoubiquitinated syntaxin 5 recruits p97/p47 to the mitotic Golgi fragments and promotes post-mitotic Golgi reassembly upon ubiquitin removal by VCIP135 (Huang et al. 2016)."

31435807

VCPIP1 affects GEMIN4

| 2

GEMIN4 binds VCPIP1-T760E. 1 / 1

| 1

➶

reach

"Much less VCIP135 (T760E, S767E) bound to GST-p97 compared with VCIP135wt (lane 1)."

23500464

GEMIN4 binds VCPIP1-S767E. 1 / 1

| 1

➶

reach

"Much less VCIP135 (T760E, S767E) bound to GST-p97 compared with VCIP135wt (lane 1)."

23500464

VCPIP1 affects FAF2

2 |

FAF2 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

19615732

➶

biogrid

No evidence text available

18775313

VCPIP1 affects DND1

2 |

DND1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

VCP affects GST

| 1 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

GST binds VCP and VCPIP1. 1 / 1

| 1

➶

sparser

"Much less VCIP135(T760E, S767E) bound to GST-p97 ( Fig. 2 B, upper panel, lane 2) compared with VCIP135wt (lane 1)."

23500464

UFD1 affects VCPIP1

2 |

UFD1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

UBXN2B affects VCPIP1

2 |

UBXN2B binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

UBXN2A affects VCPIP1

2 |

UBXN2A binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

UBC affects VCPIP1

2 |

UBC binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

23827681

➶

biogrid

No evidence text available

23500464

TTLL1 affects VCPIP1

2 |

TTLL1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

STX5 affects VCP, and VCPIP1

| 1 1

STX5 binds VCP and VCPIP1. 2 / 2

| 1 1

➶

sparser

"VCIP135 did not bind to syntaxin5 with p97 ( xref C, lane 4), a finding which was investigated further ( xref F)."

12473691

➶

reach

"Since the p97, VCIP135, and syntaxin5 complex is very unstable (XREF_FIG C), a tighter VCIP135 and p97 complex then dissociates from syntaxin5 (XREF_FIG F)."

12473691

PRMT2 affects VCPIP1

2 |

VCPIP1 binds PRMT2. 2 / 2

2 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

PLAAT5 affects VCPIP1

2 |

PLAAT5 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

P14357 affects VCPIP1

2 |

VCPIP1 binds P14357. 2 / 2

2 |

➶

virhostnet

No evidence text available

19637933

➶

virhostnet

No evidence text available

19637933

NPLOC4 affects VCPIP1

2 |

NPLOC4 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26389662

➶

biogrid

No evidence text available

19615732

MAF1 affects VCPIP1

2 |

MAF1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

GST affects VCP

| 1 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

GST binds VCP and VCPIP1. 1 / 1

| 1

➶

sparser

"Much less VCIP135(T760E, S767E) bound to GST-p97 ( Fig. 2 B, upper panel, lane 2) compared with VCIP135wt (lane 1)."

23500464

GEMIN4 affects VCPIP1

| 2

GEMIN4 binds VCPIP1-T760E. 1 / 1

| 1

➶

reach

"Much less VCIP135 (T760E, S767E) bound to GST-p97 compared with VCIP135wt (lane 1)."

23500464

GEMIN4 binds VCPIP1-S767E. 1 / 1

| 1

➶

reach

"Much less VCIP135 (T760E, S767E) bound to GST-p97 compared with VCIP135wt (lane 1)."

23500464

FAF2 affects VCPIP1

2 |

FAF2 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

19615732

➶

biogrid

No evidence text available

18775313

DND1 affects VCPIP1

2 |

DND1 binds VCPIP1. 2 / 2

2 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

28514442

Cdc2 inhibitor affects VCPIP1

| 2

Cdc2 inhibitor phosphorylates VCPIP1.

| 1

Cdc2 inhibitor leads to the phosphorylation of VCPIP1. 1 / 1

| 1

➶

reach

"The phosphorylation of VCIP135 (728-1221) was also suppressed by the Cdc2 inhibitor, olomoucine (right lane)."

23500464

Cdc2 inhibitor dephosphorylates VCPIP1.

| 1

Cdc2 inhibitor leads to the dephosphorylation of VCPIP1. 1 / 1

| 1

➶

reach

"Staurosporine (broad serine/threonine kinase inhibitor) and olomoucine (Cdc2 inhibitor) inhibited VCIP135 phosphorylation (lanes 2 and 3)."

23500464

Venlafaxine affects VCPIP1

1 |

Venlafaxine increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

25423262

Urethane affects VCPIP1

1 |

Urethane increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

28818685

Tunicamycin affects VCPIP1

1 |

Tunicamycin increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

17127020

Syntaxin5 ( affects VCPIP1

| 1

VCPIP1 binds syntaxin5 (. 1 / 1

| 1

➶

reach

"The addition of p97 decreased the amount of VCIP135 bound to syntaxin5 (XREF_FIG F)."

12473691

Syn5DeltaTM affects VCPIP1

| 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

Staurosporine affects VCPIP1

| 1

Staurosporine leads to the dephosphorylation of VCPIP1. 1 / 1

| 1

➶

reach

"Staurosporine (broad serine/threonine kinase inhibitor) and olomoucine (Cdc2 inhibitor) inhibited VCIP135 phosphorylation (lanes 2 and 3)."

23500464

Sodium arsenate affects VCPIP1

1 |

Sodium arsenate increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

21795629

RGSK‐3β‐correlated DEPs affects VCPIP1

| 1

RGSK‐3β‐correlated DEPs deubiquitinates VCPIP1. 1 / 1

| 1

➶

reach

"The rGSK‐3β‐correlated DEPs included OPTN, dehydrogenase/reductase SDR family member 4 (DHRS4), receptor protein serine/threonine kinase (TC2N), deubiquitinating protein VCIP135 (VCPIP1) (Figure 5c and f)."

34528736

RGSK-3β-correlated DEPs affects VCPIP1

| 1

RGSK-3β-correlated DEPs deubiquitinates VCPIP1. 1 / 1

| 1

➶

reach

"The rGSK-3β-correlated DEPs included OPTN, dehydrogenase/reductase SDR family member 4 (DHRS4), receptor protein serine/threonine kinase (TC2N), deubiquitinating protein VCIP135 (VCPIP1) (Figure 5c and f)."

34528736

Phorbol 13-acetate 12-myristate affects VCPIP1

1 |

Phorbol 13-acetate 12-myristate decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

25613284

Phenethyl isothiocyanate affects VCPIP1

| 1

Phenethyl isothiocyanate inhibits VCPIP1. 1 / 1

| 1

➶

reach

"To our knowledge, the inhibition of VCPIP1 by PEITC is the first report of inhibition of an OTU domain DUB."

28881649

Phenethyl caffeate affects VCPIP1

1 |

Phenethyl caffeate decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

20360939

Nocodazole affects VCPIP1

| 1

Nocodazole activates VCPIP1. 1 / 1

| 1

➶

reach

"The samples were analyzed by SDS-PAGE and western blotting.For deubiquitination of Syn5 by immunoprecipitated VCIP135, HeLa cells were treated with 100 ng/ml nocodazole (Sigma) for 18 hr, washed five [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

27404360

Miconazole affects VCPIP1

1 |

Miconazole decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27462272

Ketamine affects VCPIP1

1 |

Ketamine decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

20080153

Jinfukang affects VCPIP1

1 |

Jinfukang decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27392435

Ionomycin affects VCPIP1

1 |

Ionomycin decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

25613284

Importin affects alpha 1

| 1

PLSCR4 binds VCPIP1, alpha 1, and importin. 1 / 1

| 1

➶

sparser

"The only other functional information about the N2 protein comes from a yeast-2 hybrid screen ( xref ) which suggested that N2 could bind directly to importin alpha 1, VCPIP1 and PLSCR4, although it is currently unclear how these putative interaction partners may contribute to the function of N2 described here."

23761407

Hsa-miR-943 affects VCPIP1

1 |

Hsa-miR-943 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-92a-3p affects VCPIP1

1 |

Hsa-miR-92a-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-877-3p affects VCPIP1

1 |

Hsa-miR-877-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-7977 affects VCPIP1

1 |

Hsa-miR-7977 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-675-5p affects VCPIP1

1 |

Hsa-miR-675-5p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-583 affects VCPIP1

1 |

Hsa-miR-583 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-5002-3p affects VCPIP1

1 |

Hsa-miR-5002-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-4801 affects VCPIP1

1 |

Hsa-miR-4801 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-4746-3p affects VCPIP1

1 |

Hsa-miR-4746-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-4731-3p affects VCPIP1

1 |

Hsa-miR-4731-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-4639-5p affects VCPIP1

1 |

Hsa-miR-4639-5p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-4466 affects VCPIP1

1 |

Hsa-miR-4466 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-4439 affects VCPIP1

1 |

Hsa-miR-4439 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-4311 affects VCPIP1

1 |

Hsa-miR-4311 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-4284 affects VCPIP1

1 |

Hsa-miR-4284 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-383-3p affects VCPIP1

1 |

Hsa-miR-383-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-361-5p affects VCPIP1

1 |

Hsa-miR-361-5p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-3136-5p affects VCPIP1

1 |

Hsa-miR-3136-5p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

19536157

Hsa-miR-24-3p affects VCPIP1

1 |

Hsa-miR-24-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-193b-3p affects VCPIP1

1 |

Hsa-miR-193b-3p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hsa-miR-1827 affects VCPIP1

1 |

Hsa-miR-1827 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-155-5p affects VCPIP1

1 |

Hsa-miR-155-5p decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

22473208

Hsa-miR-1276 affects VCPIP1

1 |

Hsa-miR-1276 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23824327

Hsa-miR-1260b affects VCPIP1

1 |

Hsa-miR-1260b decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:mirtarbase

No evidence text available

23622248

Hexabromocyclododecane affects VCPIP1

1 |

Hexabromocyclododecane decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

29177809

Glycitin affects VCPIP1

1 |

Glycitin increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27424125

Glycitein affects VCPIP1

1 |

Glycitein increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27424125

Genistein affects VCPIP1

1 |

Genistein increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27424125

Genistein 7-O-beta-D-glucoside affects VCPIP1

1 |

Genistein 7-O-beta-D-glucoside increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27424125

Formaldehyde affects VCPIP1

1 |

Formaldehyde decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

20655997

Fenofibrate affects VCPIP1

1 |

Fenofibrate increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

21318169

Double-stranded RNA affects VCPIP1

| 1

Double-stranded RNA deubiquitinates VCPIP1. 1 / 1

| 1

➶

reach

"Deubiquitinating protein VCIP135, the F-actin-capping protein sub-unit alpha-2, and insulin-like growth factor 2 mRNA-binding protein 2 were biotinylated via AirID fused with OR, RTP1S-AirID biotinylated heat shock protein A6 (HSPA6), and double-stranded RNA-binding protein Staufen homolog 2 (STAU2)."

37175532

Diphenylcyclopropenone affects VCPIP1

| 1

Diphenylcyclopropenone activates VCPIP1. 1 / 1

| 1

➶

reach

"Indeed, mechanistically, DPC induction triggers VCPIP1 activation by DNA damage-responsive kinases ATM/ATR, which then deubiquitinates SPRTN to repair DPCs."

32649882

Diarsenic trioxide affects VCPIP1

1 |

Diarsenic trioxide increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

20458559

Daidzein affects VCPIP1

1 |

Daidzein increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27424125

Daidzein 7-O-beta-D-glucoside affects VCPIP1

1 |

Daidzein 7-O-beta-D-glucoside increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27424125

Copper(II) sulfate affects VCPIP1

1 |

Copper(II) sulfate increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

19549813

Cobalt dichloride affects VCPIP1

1 |

Cobalt dichloride increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

24386269

Clorgyline affects VCPIP1

1 |

Clorgyline increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

19691856

Cisplatin affects VCPIP1

1 |

Cisplatin decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

27594783

Cadmium dichloride affects VCPIP1

1 |

Cadmium dichloride methylates VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

22457795

Butanal affects VCPIP1

1 |

Butanal decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

26079696

Broad serine/threonine kinase inhibitor affects VCPIP1

| 1

Broad serine/threonine kinase inhibitor leads to the dephosphorylation of VCPIP1. 1 / 1

| 1

➶

reach

"Staurosporine (broad serine/threonine kinase inhibitor) and olomoucine (Cdc2 inhibitor) inhibited VCIP135 phosphorylation (lanes 2 and 3)."

23500464

Bis(2-ethylhexyl) phthalate affects VCPIP1

1 |

Bis(2-ethylhexyl) phthalate decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

16400681

Benzo[a]pyrene affects VCPIP1

1 |

Benzo[a]pyrene increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

20064835

Beclomethasone affects VCPIP1

1 |

Beclomethasone decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

23656298

Alpha 1 affects importin

| 1

PLSCR4 binds VCPIP1, alpha 1, and importin. 1 / 1

| 1

➶

sparser

23761407

ZIC2 affects VCPIP1

1 |

ZIC2 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

WAC affects VCP

| 1

VCP binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

"We therefore investigated the effect of phosphorylation on the binding of VCIP135 to p97 or WAC."

23500464

WAC affects GST

| 1

GST binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

23500464

Vehicle Emissions affects VCPIP1

1 |

Vehicle Emissions methylates VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

25560391

VCPIP1 affects syntaxin5 (

| 1

VCPIP1 binds syntaxin5 (. 1 / 1

| 1

➶

reach

"The addition of p97 decreased the amount of VCIP135 bound to syntaxin5 (XREF_FIG F)."

12473691

VCPIP1 affects syn5DeltaTM

| 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

VCPIP1 affects relocalization SPRTN

| 1

VCPIP1 activates relocalization SPRTN. 1 / 1

| 1

➶

eidos

"Indeed , upon DPC induction , knockdown of VCPIP1 reduced the relocalization of SPRTN pool to chromatin ( Figure 2E ) , whereas VCPIP1 reconstitution reversed this phenotype ( Figures 2F and S2C ) ."

32649882

VCPIP1 affects premature aging mice

| 1

VCPIP1 inhibits premature aging mice. 1 / 1

| 1

➶

eidos

"These results indicate that loss of VCPIP1 promotes premature aging in mice ."

32649882

VCPIP1 affects pol

| 1

VCPIP1 phosphorylates pol. 1 / 1

| 1

➶

sparser

25904330

VCPIP1 affects p97ATPase

| 1

VCPIP1 inhibits p97ATPase. 1 / 1

| 1

➶

reach

"Mitotic phosphorylation of VCIP135 blocks p97ATPase mediated Golgi membrane fusion."

23500464

VCPIP1 affects p97-p47

| 1

VCPIP1 deubiquitinates p97-p47. 1 / 1

| 1

➶

reach

"The intimate interaction of the three factors, however, suggests a mechanism that is dependent on the recognition and processing of a ubiquitin conjugate by p97-p47 and subsequent deubiquitination by VCIP135."

15037600

VCPIP1 affects interaction

| 1

VCPIP1 activates interaction. 1 / 1

| 1

➶

eidos

32649882

VCPIP1 affects importin

| 1

PLSCR4 binds VCPIP1, alpha 1, and importin. 1 / 1

| 1

➶

sparser

23761407

VCPIP1 affects function

| 1

VCPIP1 inhibits function. 1 / 1

| 1

➶

sparser

"TNFAIP3 , also called A20, is a deubiquitinating protein that can inhibit the function of a critical transcription factor, NF-κB and, hence, regulate expression of all downstream genes."

27338350

VCPIP1 affects elaD

| 1

VCPIP1 inhibits elaD. 1 / 1

| 1

➶

reach

"Mitotic phosphorylation of VCIP135 at S130 inhibits its deubiquitinase activity."

25904330

VCPIP1 affects diphenylcyclopropenone

| 1

VCPIP1 inhibits diphenylcyclopropenone. 1 / 1

| 1

➶

reach

"Loss of VCPIP1 resulted in cellular hypersensitivity to DPC-inducing agents, as well as Top1cc and DPC accumulation, suggesting that VCPIP1 plays a critical role in DPC repair."

32649882

VCPIP1 affects deubiquitination induced FA treatment

| 1

VCPIP1 activates deubiquitination induced FA treatment. 1 / 1

| 1

➶

eidos

"Importantly , as shown in Figures 2B and S2B , knockdown of VCPIP1 abolished the deubiquitination induced by FA treatment , suggesting that DPC-induced deubiquitination of SPRTN is dependent on VCPIP1 ."

32649882

VCPIP1 affects chromatin relocalization

| 1

VCPIP1 activates chromatin relocalization. 1 / 1

| 1

➶

eidos

"VCPIP1 , in turn , deubiquitinates SPRTN and promotes its chromatin relocalization ."

32649882

VCPIP1 affects cellular hypersensitivity DPC-inducing

| 1

VCPIP1 inhibits cellular hypersensitivity DPC-inducing. 1 / 1

| 1

➶

eidos

"Loss of VCPIP1 resulted in cellular hypersensitivity to DPC-inducing agents , as well as Top1cc and DPC accumulation , suggesting that VCPIP1 plays a critical role in DPC repair ."

32649882

VCPIP1 affects association SPRTN

| 1

VCPIP1 activates association SPRTN. 1 / 1

| 1

➶

eidos

"VCPIP1 is phosphorylated by ATM / ATR upon DPC induction Our results so far suggest that VCPIP1 deubiquitinates SPRTN , thereby triggering association of SPRTN with chromatin following DPC damage ."

32649882

VCPIP1 affects alphaSnap

| 1

VCPIP1 activates alphaSnap. 1 / 1

| 1

➶

reach

"As shown in XREF_FIG G, VCIP135 partially prevented alpha-SNAP binding to GST-syn5DeltaTM, suggesting that VCIP135 directs syntaxin5 to p97 and p47 but not to alpha-SNAP and NSF."

12473691

VCPIP1 affects alpha 1

| 1

PLSCR4 binds VCPIP1, alpha 1, and importin. 1 / 1

| 1

➶

sparser

23761407

VCPIP1 affects acetylation SPRTN

| 1

VCPIP1 activates acetylation SPRTN. 1 / 1

| 1

➶

eidos

"We found that knockdown of VCPIP1 decreased acetylation of SPRTN upon DPC induction ( Figure 4I ) , suggesting that SPRTN acetylation requires SPRTN deubiquitination ."

32649882

VCPIP1 affects VAV2

1 |

VAV2 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18654987

VCPIP1 affects Ubx

| 1

VCP binds VCPIP1 and Ubx. 1 / 1

| 1

➶

sparser

28819009

VCPIP1 affects USP11

| 1

USP11 binds VCPIP1. 1 / 1

| 1

➶

sparser

"VCPIP1 and USP11 interact weakly with SPRTN in co-immunoprecipitating experiments, but show no (VCPIP1) or only weak (USP11) preference for SPRTN-Ub ( xref )."

33348378

VCPIP1 affects UBXN8

1 |

UBXN8 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

VCPIP1 affects UBXN7

1 |

UBXN7 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26389662

VCPIP1 affects UBXN6

1 |

UBXN6 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

VCPIP1 affects UBXN11

1 |

UBXN11 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

VCPIP1 affects UBXN1

1 |

UBXN1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

VCPIP1 affects UBX-L domain

| 1

VCP binds VCPIP1 and UBX-L domain. 1 / 1

| 1

➶

reach

"The DUB VCIP135, central to the regulation of membrane fusion, also binds p97 via a UBX-L domain [XREF_BIBR]."

28819009

VCPIP1 affects Top1cc foci

| 1

VCPIP1 inhibits Top1cc foci. 1 / 1

| 1

➶

reach

"Knockout of Vcpip1 also enhanced the accumulation of Top1cc foci signals (Figures 5B–5C), and impeded spontaneous DPC repair ability (Figure 5D), indicating that VCPIP1 deficiency results in failure to repair DPCs."

32649882

VCPIP1 affects Top1cc accumulation

| 1

VCPIP1 inhibits Top1cc accumulation. 1 / 1

| 1

➶

eidos

"Loss of VCPIP1 resulted in cellular hypersensitivity to DPC-inducing agents , as well as Top1cc and DPC accumulation , suggesting that VCPIP1 plays a critical role in DPC repair ."

32649882

VCPIP1 affects TRAF6

| 1

VCPIP1 inhibits TRAF6. 1 / 1

| 1

➶

sparser

"Tumor necrosis factor alpha-induced protein 3 is a deubiquitinating protein that inhibits TRAF6 and thus prevents TRAF6 from ubiquitinating a key autophagic protein Beclin-1 ( xref )."

32117140

VCPIP1 affects SVIP

1 |

SVIP binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

19615732

VCPIP1 affects STIL

1 |

STIL binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26638075

VCPIP1 affects SSX2IP

1 |

SSX2IP binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26638075

VCPIP1 affects SPRTN-mediated DPC repair

| 1

VCPIP1 activates SPRTN-mediated DPC repair. 1 / 1

| 1

➶

eidos

"Deubiquitination of SPRTN by VCPIP1 is important for DPC repair We hypothesized that VCPIP1 deubiquitinates SPRTN and promotes SPRTN-mediated DPC repair ."

32649882

VCPIP1 affects SPRTN localization

| 1

VCPIP1 activates SPRTN localization. 1 / 1

| 1

➶

eidos

"A previous study showed that VCPIP1 deubiquitinates SPRTN , promoting SPRTN localization to chromatin ( 35 ) ."

33567341

VCPIP1 affects SPRTN deubiquitylation

| 1

VCPIP1 activates SPRTN deubiquitylation. 1 / 1

| 1

➶

eidos

"Neither VCPIP1 nor USP11 induce SPRTN deubiquitylation when overexpressed , while USP7 does ( Supplementary Figure S3B ) ."

33348378

VCPIP1 affects SMAD9

1 |

VCPIP1 binds SMAD9. 1 / 1

1 |

➶

biogrid

No evidence text available

15231748

VCPIP1 affects SHBG

| 1

SHBG binds VCPIP1. 1 / 1

| 1

➶

reach

"To determine whether Cdk1 directly phosphorylates VCIP135 on S130, we performed an in vitro phosphorylation assay with streptavidin binding peptide (SBP)-tagged VCIP135 recombinant proteins."

25904330

VCPIP1 affects PLSCR4

| 1

PLSCR4 binds VCPIP1, alpha 1, and importin. 1 / 1

| 1

➶

sparser

23761407

VCPIP1 affects OTU domain

| 1

VCPIP1 binds CDC48 and OTU domain. 1 / 1

| 1

➶

reach

"S. cerevisiae Otu1p also physically interacts with Cdc48p XREF_BIBR and human CDC48 (VCP and p97) binds to VCIP135, an OTU domain DUB XREF_BIBR."

20838651

VCPIP1 affects OLFM2

1 |

OLFM2 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects NXF1

1 |

VCPIP1 binds NXF1. 1 / 1

1 |

➶

biogrid

No evidence text available

22658674

VCPIP1 affects NTRK1

1 |

VCPIP1 binds NTRK1. 1 / 1

1 |

➶

biogrid

No evidence text available

25921289

VCPIP1 affects NR0B2

1 |

VCPIP1 binds NR0B2. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects Mice

| 1

VCPIP1 inhibits Mice. 1 / 1

| 1

➶

reach

"These results indicate that loss of VCPIP1 promotes premature aging in mice."

32649882

VCPIP1 affects MOV10

1 |

VCPIP1 binds MOV10. 1 / 1

1 |

➶

biogrid

No evidence text available

22658674

VCPIP1 affects MED23

1 |

MED23 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

25281560

VCPIP1 affects LRCH3

1 |

LRCH3 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects LGALS3BP

1 |

VCPIP1 binds LGALS3BP. 1 / 1

1 |

➶

biogrid

No evidence text available

26496610

VCPIP1 affects LARP7

1 |

LARP7 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

22863883

VCPIP1 affects KIF20B

1 |

VCPIP1 binds KIF20B. 1 / 1

1 |

➶

biogrid

No evidence text available

26496610

VCPIP1 affects KAT2B

| 1

VCPIP1 binds KAT2A and KAT2B. 1 / 1

| 1

➶

sparser

"Interestingly, VCPIP1 interacted with PCAF and GCN5 in a DPC damage-inducible manner ( xref )."

32649882

VCPIP1 affects HUWE1

1 |

HUWE1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

19615732

VCPIP1 affects HBx

| 1

VCPIP1 activates HBx. 1 / 1

| 1

➶

eidos

"PSMC3 , encoding the 26S proteasome regulatory subunit , directly stabilized HBx through physical binding instead of a common approach in protein degradation , serving as the key downstream effector of VCPIP1 on HBx ."

35695579

VCPIP1 affects HBx protein

| 1

VCPIP1 activates HBx protein. 1 / 1

| 1

➶

eidos

"VCPIP1 and its C-terminal amino acids 863 to 1221 upregulated the HBx protein expression , with or without HBV infection ."

35695579

VCPIP1 affects GAN

1 |

VCPIP1 binds GAN. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects FIGNL1

1 |

FIGNL1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects FCHSD2

1 |

FCHSD2 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18654987

VCPIP1 affects FAF1

1 |

VCPIP1 binds FAF1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

VCPIP1 affects EDEM3

1 |

EDEM3 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28366632

VCPIP1 affects DPC-dependent manner

| 1

VCPIP1 activates DPC-dependent manner. 1 / 1

| 1

➶

eidos

32649882

VCPIP1 affects CNKSR3

1 |

CNKSR3 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects CENPJ

1 |

CENPJ binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26638075

VCPIP1 affects CDH1

1 |

CDH1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

25468996

VCPIP1 affects CDC48

| 1

VCPIP1 binds CDC48 and OTU domain. 1 / 1

| 1

➶

reach

"S. cerevisiae Otu1p also physically interacts with Cdc48p XREF_BIBR and human CDC48 (VCP and p97) binds to VCIP135, an OTU domain DUB XREF_BIBR."

20838651

VCPIP1 affects CDC20B

1 |

CDC20B binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects BTRC

1 |

BTRC binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

25900982

VCPIP1 affects Aging, Premature

| 1

VCPIP1 inhibits Aging, Premature. 1 / 1

| 1

➶

reach

"These results indicate that loss of VCPIP1 promotes premature aging in mice."

32649882

VCPIP1 affects ATP13A1

1 |

ATP13A1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

VCPIP1 affects ASPSCR1

1 |

ASPSCR1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

VCPIP1 affects ABCC12

1 |

ABCC12 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

19615732

VCP affects syn5DeltaTM

| 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

VCP affects WAC

| 1

VCP binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

"We therefore investigated the effect of phosphorylation on the binding of VCIP135 to p97 or WAC."

23500464

VCP affects Ubx

| 1

VCP binds VCPIP1 and Ubx. 1 / 1

| 1

➶

sparser

28819009

VCP affects UBX-L domain

| 1

VCP binds VCPIP1 and UBX-L domain. 1 / 1

| 1

➶

reach

"The DUB VCIP135, central to the regulation of membrane fusion, also binds p97 via a UBX-L domain [XREF_BIBR]."

28819009

VAV2 affects VCPIP1

1 |

VAV2 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18654987

Ubx affects VCPIP1

| 1

VCP binds VCPIP1 and Ubx. 1 / 1

| 1

➶

sparser

28819009

USP11 affects VCPIP1

| 1

USP11 binds VCPIP1. 1 / 1

| 1

➶

sparser

"VCPIP1 and USP11 interact weakly with SPRTN in co-immunoprecipitating experiments, but show no (VCPIP1) or only weak (USP11) preference for SPRTN-Ub ( xref )."

33348378

UBXN8 affects VCPIP1

1 |

UBXN8 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

UBXN7 affects VCPIP1

1 |

UBXN7 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26389662

UBXN6 affects VCPIP1

1 |

UBXN6 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

UBXN11 affects VCPIP1

1 |

UBXN11 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

UBXN1 affects VCPIP1

1 |

UBXN1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

UBX-L domain affects VCPIP1

| 1

VCP binds VCPIP1 and UBX-L domain. 1 / 1

| 1

➶

reach

"The DUB VCIP135, central to the regulation of membrane fusion, also binds p97 via a UBX-L domain [XREF_BIBR]."

28819009

U0126 affects VCPIP1

| 1

U0126 activates VCPIP1. 1 / 1

| 1

➶

reach

25904330

T-2 Toxin affects VCPIP1

1 |

T-2 Toxin decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

31299295

SVIP affects VCPIP1

1 |

SVIP binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

19615732

STIL affects VCPIP1

1 |

STIL binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26638075

SSX2IP affects VCPIP1

1 |

SSX2IP binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

26638075

SMAD9 affects VCPIP1

1 |

VCPIP1 binds SMAD9. 1 / 1

1 |

➶

biogrid

No evidence text available

15231748

SHBG affects VCPIP1

| 1

SHBG binds VCPIP1. 1 / 1

| 1

➶

reach

"To determine whether Cdk1 directly phosphorylates VCIP135 on S130, we performed an in vitro phosphorylation assay with streptavidin binding peptide (SBP)-tagged VCIP135 recombinant proteins."

25904330

Plant Oils affects VCPIP1

1 |

Plant Oils increases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

23370395

PLSCR4 affects importin

| 1

PLSCR4 binds VCPIP1, alpha 1, and importin. 1 / 1

| 1

➶

sparser

23761407

PAX5 affects VCPIP1

1 |

PAX5 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

OTU domain affects VCPIP1

| 1

VCPIP1 binds CDC48 and OTU domain. 1 / 1

| 1

➶

reach

"S. cerevisiae Otu1p also physically interacts with Cdc48p XREF_BIBR and human CDC48 (VCP and p97) binds to VCIP135, an OTU domain DUB XREF_BIBR."

20838651

OLFM2 affects VCPIP1

1 |

OLFM2 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

NXF1 affects VCPIP1

1 |

VCPIP1 binds NXF1. 1 / 1

1 |

➶

biogrid

No evidence text available

22658674

NTRK1 affects VCPIP1

1 |

VCPIP1 binds NTRK1. 1 / 1

1 |

➶

biogrid

No evidence text available

25921289

NSFL1C affects syn5DeltaTM

| 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

NRF1 affects VCPIP1

1 |

NRF1 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

NR0B2 affects VCPIP1

1 |

VCPIP1 binds NR0B2. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

N-nitrosodiethylamine affects VCPIP1

1 |

N-nitrosodiethylamine decreases the amount of VCPIP1. 1 / 1

1 |

➶

ctd

No evidence text available

20360939

MYB affects VCPIP1

1 |

MYB decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

MOV10 affects VCPIP1

1 |

VCPIP1 binds MOV10. 1 / 1

1 |

➶

biogrid

No evidence text available

22658674

MED23 affects VCPIP1

1 |

MED23 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

25281560

LRCH3 affects VCPIP1

1 |

LRCH3 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

LGALS3BP affects VCPIP1

1 |

VCPIP1 binds LGALS3BP. 1 / 1

1 |

➶

biogrid

No evidence text available

26496610

LARP7 affects VCPIP1

1 |

LARP7 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

22863883

KIF20B affects VCPIP1

1 |

VCPIP1 binds KIF20B. 1 / 1

1 |

➶

biogrid

No evidence text available

26496610

KAT2B affects VCPIP1

| 1

VCPIP1 binds KAT2A and KAT2B. 1 / 1

| 1

➶

sparser

"Interestingly, VCPIP1 interacted with PCAF and GCN5 in a DPC damage-inducible manner ( xref )."

32649882

Infections affects VCPIP1

| 1

Infections increases the amount of VCPIP1. 1 / 1

| 1

➶

reach

"In the present study, DTMUV infection induced differential expression of the deubiquitinating protein VCIP135, proteasome 26S subunit, and proteasome subunit beta."

27066001

HUWE1 affects VCPIP1

1 |

HUWE1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

19615732

HSPA6 affects VCPIP1

| 1

HSPA6 deubiquitinates VCPIP1. 1 / 1

| 1

➶

reach

37175532

HNF4A affects VCPIP1

1 |

HNF4A decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

GST affects syn5DeltaTM

| 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

GST affects WAC

| 1

GST binds WAC and VCPIP1. 1 / 1

| 1

➶

sparser

23500464

GST affects VCP, and VCPIP1

| 1

GST binds VCP and VCPIP1. 1 / 1

| 1

➶

sparser

"Much less VCIP135(T760E, S767E) bound to GST-p97 ( Fig. 2 B, upper panel, lane 2) compared with VCIP135wt (lane 1)."

23500464

GST affects NSFL1C, VCP, VCPIP1, and syn5DeltaTM

| 1

GST binds VCP, NSFL1C, VCPIP1, and syn5DeltaTM. 1 / 1

| 1

➶

reach

12473691

GAN affects VCPIP1

1 |

VCPIP1 binds GAN. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

FOXO4 affects VCPIP1

1 |

FOXO4 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

FIGNL1 affects VCPIP1

1 |

FIGNL1 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28514442

FCHSD2 affects VCPIP1

1 |

FCHSD2 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

18654987

FAF1 affects VCPIP1

1 |

VCPIP1 binds FAF1. 1 / 1

1 |

➶

biogrid

No evidence text available

18775313

ELK1 affects VCPIP1

1 |

ELK1 decreases the amount of VCPIP1. 1 / 1

1 |

➶

biopax:msigdb

No evidence text available

EDEM3 affects VCPIP1

1 |

EDEM3 binds VCPIP1. 1 / 1

1 |

➶

biogrid

No evidence text available

28366632

DNA kinases affects VCPIP1

| 1

DNA kinases inhibits VCPIP1. 1 / 1

| 1

➶

eidos

"Indeed , mechanistically , DPC induction triggers VCPIP1 activation by DNA damage-responsive kinases ATM / ATR , which then deubiquitinates SPRTN to repair DPCs ."

32649882