IndraLab

Statements

VCPIP1 activates X. 9 / 9
| 1 8
reach
"Although VCPIP1 overexpression led to significantly increased HBx in the Huh7 and HepG2 cells, it did not redice the amounts of HBx ubiquitination (Fig. 4C)."
35695579
reach
"RNA interference (RNAi) targeting endogenous PSMC3 was used to validate that PSMC3 was required for VCPIP1-mediated HBx stability but was not a contributing factor."
35695579
reach
"Collectively, the results indicate that VCPIP1-recruited PSMC3 associating with HBx functionally prevents the HBx degradation by the 20S proteasome."
35695579
reach
"As expected, ectopic VCPIP1 expression led to significantly increased HBx in a dose-dependent manner in both Huh7 and HepG2 cells (Fig. 2B)."
35695579
reach
"Then, the similar effects of VCPIP1 and its C-terminal truncation increasing the HBx expression were validated in HepG2.2.15 cells (Fig. 2G), but HBx was reduced by silencing endogenous VCPIP1 (Fig. 2H)."
35695579
reach
"VCPIP1 increases HBx stability.."
35695579
reach
"VCPIP1 inhibits HBx degradation through the proteasome pathway in a ubiquitin-independent manner.."
35695579
eidos
"PSMC3 , encoding the 26S proteasome regulatory subunit , directly stabilized HBx through physical binding instead of a common approach in protein degradation , serving as the key downstream effector of VCPIP1 on HBx ."
35695579
reach
"Finally, the VCPIP1-induced HBx stability greatly promoted its canonical transcriptional activities and contributed to the inhibition of colony formation of Huh7 and HepG2 cells."
35695579