IndraLab

Statements

STX5 binds VCPIP1. 22 / 30
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sparser
"VCIP135 bound to syntaxin5 is thought to associate with a syntaxin5-bound p97/p47 complex with syntaxin5 acting as a scaffold for the p97/p47 dissociation reaction."
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reach
"The binding of VCIP135 to syntaxin5 independently of p47 (XREF_FIG C) allows VCIP135 to be closely associated with the p97 and p47 complex, whereas in cytosol such an association is less likely."
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sparser
"The specificity of binding of VCIP135 to syntaxin5 was further confirmed by the coimmunoprecipitation experiments using Golgi membranes as shown in xref B."
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sparser
"These data suggest that both VCIP135 and the p97/p47 complex bind to syntaxin5, resulting in the formation of a transient quaternary complex that could be dissociated through p97 ATP hydrolysis."
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reach
"These data suggest that both VCIP135 and the p97 and p47 complex bind to syntaxin5, resulting in the formation of a transient quaternary complex that could be dissociated through p97 ATP hydrolysis."
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sparser
"Although VCIP135 can bind to p97 in cytosol ( xref A), VCIP135 bound to syntaxin5 does not form a complex with p97 ( xref C, lane 4)."
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sparser
"VCIP135 forms a quaternary complex with syntaxin 5 and the p97/p47 complex via a ubiquitin fold domain and its enzymatic activity is required for post-mitotic Golgi membrane fusion xref ."
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sparser
"However, in the presence of AMP–PNP or ADP, p97 and p47 bound to syntaxin5 even when VCIP135 was added, and VCIP135 also bound to syntaxin5 ( xref D, right six lanes)."
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sparser
"We have shown that VCIP135 binds to syntaxin5 and disassembles the p97/p47/syntaxin5 complex through p97 ATP hydrolysis."
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biogrid
No evidence text available
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hprd
No evidence text available
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biogrid
No evidence text available
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sparser
"The addition of p97 decreased the amount of VCIP135 bound to syntaxin5 ( xref F)."
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reach
"Although VCIP135 can bind to p97 in cytosol (XREF_FIG A), VCIP135 bound to syntaxin5 does not form a complex with p97 (XREF_FIG C, lane 4)."
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reach
"Hence, we investigated the interaction between VCIP135 and syntaxin5 by binding experiments using GST tagged syntaxin5 without the transmembrane domain (GST-syn5DeltaTM) as shown in XREF_FIG A."
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reach
"VCIP135 bound to syntaxin5 is thought to associate with a syntaxin5 bound p97 and p47 complex with syntaxin5 acting as a scaffold for the p97 and p47 dissociation reaction."
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reach
"We have shown that VCIP135 binds to syntaxin5 and disassembles the p97, p47, and syntaxin5 complex through p97 ATP hydrolysis."
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sparser
"In the presence of ATP, the addition of VCIP135 prevented p97 and p47 from binding to syntaxin5 and VCIP135 itself only bound poorly to syntaxin5 ( xref D, left three lanes)."
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sparser
"Syntaxin5 was coimmunoprecipitated ( xref B), providing evidence that VCIP135 binds to syntaxin5 in Golgi membranes."
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reach
"The specificity of binding of VCIP135 to syntaxin5 was further confirmed by the coimmunoprecipitation experiments using Golgi membranes as shown in XREF_FIG B. 1 M KCl washed Golgi membranes were incubated with His tagged VCIP135."
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sparser
"Hence, we investigated the interaction between VCIP135 and syntaxin5 by binding experiments using GST-tagged syntaxin5 without the transmembrane domain (GST–syn5ΔTM) as shown in xref A. Since syntaxin1 is a SNARE in plasma membranes, we used GST–syn1ΔTM as a negative control."
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sparser
"The binding of VCIP135 to syntaxin5 independently of p47 ( xref C) allows VCIP135 to be closely associated with the p97/p47 complex, whereas in cytosol such an association is less likely."
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