IndraLab

Statements

VCPIP1 binds MYBL1. 14 / 14
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"MYB was rearranged in 60% (12/20), MYBL1 in 30% (6/20) and NFIB in 5% (1/20); two tumors exhibited novel fusion products, including NFIB-TBPL1 and MYBL1-VCPIP1 ."
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"However, Bubola et al. [ xref ] identified a MYBL1-VCPIP1 fusion product following the genomic characterization of salivary gland tumors."
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"The VCPIP1 gene is particularly interesting to our research because it is (a) downregulated when we silence MYBL1 in MDA-MB-231 cells, (b) it is located at the 8q13.1 chromosomal locus, like MYBL1, (c) it is dysregulated in many of the same TNBC patient samples as MYBL1, (d) bioinformatic analyses indicate MYBL1 transcription factor protein binding to the VCPIP1 gene, and (e) both genes are involved in cell cycle signaling mechanisms."
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"In addition, two cases were found to harbor novel fusion genes ( NFIB-TBPL1 and MYBL1-VCPIP1 )."
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"It could be, but we are examining the possibility that the MYBL1 transcription factor directly binds to VCPIP1 promoter allowing for direct regulation in TNBC."
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"The Electrophoretic Mobility Shift Assay (EMSA) is performed to show that the MYBL1 transcription factor can bind to the promoter region of the VCPIP1 gene."
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"In contrast, other than the identification of a MYBL1-VCPIP1 gene fusion event in salivary gland tumors [ xref ], a possible relationship between MYBL1 and VCPIP1 genes has not been established."
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"Adenoid cystic carcinoma is characterized by a recurrent t(6;9)(q22–23;p23–24) translocation that results in a MYB-NFIB fusion gene. xref , xref An alternative MYBL1-NFIB gene fusion has been reported in a subset of cases. xref , xref , xref Various other genes have been reported to substitute for MYB , MYBL1 and NFIB , including TGFRB3, RAD51B, YTHDF3, AIG1, XRCC4 and PTPRD , amongst others. xref , xref , xref – xref In our cohort of 20 AdCCs, the MYB-NFIB fusion was identified in 60% and the MYBL1-NFIB fusion in 25%, while two cases harbored novel NFIB-TBPL1 and MYBL1-VCPIP1 fusions products."
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"Co-Expression of MYBL1 and VCPIP1 Genes and Experimental Analyses of MYBL1 Transcription Factor Binding to VCPIP1 Promoter."
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"In addition, because bioinformatic analyses suggest the possible regulation of VCPIP1 by the MYBL1 transcription factor, we decided to experimentally examine the possibility that MYBL1 binds directly to the VCPIP1 promoter region, explaining our previous gene expression results."
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"Other than the fact that MYBL1 and VCPIP1 genes are associated with cell cycle signaling events and a MYBL1-VCPIP1 fusion product has been observed in salivary gland tumors, no experimental data support a relationship between the two genes."
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"RNA-Seq identified MYBL1-NFIB , NFIB-TBPL1 , and MYBL1-VCPIP1 fusion transcripts, respectively, supporting the diagnosis of AdCC in these three tumors."
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"The purified MYBL1 protein only bound to one of the putative VCPIP1 sequences ( xref ), the sequence given in xref B. To validate the performance of the EMSA assay, as a control, the Epstein–Barr virus protein was shown to bind to its specific DNA probe sequence (lane 1)."
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"Experimental results of the EMSA assay show that the purified MYBL1 recombinant protein binds to the sequence ~940 nucleotides upstream of the VCPIP1 start site."
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