IndraLab

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ZUP1 affects SIRT2

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ZUP1 increases the amount of SIRT2.

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ZUP1 increases the amount of SIRT2. 7 / 7

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"We firstly found that DUb upregulated the SIRT2 expression in LoVo cells, but DUb did not affect SIRT2 expression after SIRT2 was silenced (Figure 6A)."

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"We also found that DUb upregulated SIRT2 expression in DUb-treated HCT116 and LoVo cells and CRC tissues treated with DUb."

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"*p < 0.05; **p < 0.01; ***p < 0.001.FIGURE 5: DUb increases the expression of SIRT2."

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"DUb significantly induced mRNA expression of SIRT2 in HCT116 (B) and LoVo cells (C)."

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"(F) The results of western blotting showed that DUb markedly increased the protein expression of SIRT2 in the CT26 xenograft tumors and patient-derived xenograft (PDX) tumors (n = 3)."

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"(G) The results of immunohistochemical staining showed that DUb markedly increased the protein expression of SIRT2 in the CT26 xenograft tumors and patient-derived xenograft (PDX) tumors (n = 3)."

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"Furthermore, the results of IHC staining against SIRT2 antibodies shows that SIRT2 expression in the DUb-treated xenograft tumor is significantly increased as compared with control.Given that DUb upregulated SIRT2 expression, we speculated that DUb inhibited CRC growth by upregulating SIRT2."

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ZUP1 inhibits SIRT2.

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ZUP1 inhibits SIRT2. 3 / 3

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"It was further demonstrated that DUb inhibited LoVo cell proliferation, migration and invasion by upregulating SIRT2."

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"In the present study, we reported that DUb inhibited CRC growth by upregulating SIRT2."

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ZUP1 decreases the amount of SIRT2.

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ZUP1 decreases the amount of SIRT2. 1 / 1

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"In this study, we report an important finding that DUb, a coenzyme Q10 analog, can potently suppress CRC cell proliferation, migration and invasion by up-regulating SIRT2 expression."

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ZUP1 affects cell population proliferation

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ZUP1 inhibits cell population proliferation.

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ZUP1 inhibits cell population proliferation. 6 / 6

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"(A) After HCT116 and LoVo cells were treated with DUb or DMSO for 96 h, DUb suppressed the proliferation of HCT116 and LoVo cells (n = 12)."

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"We demonstrated that DUb significantly reduced the proliferation of HCT116 and LoVo cells in a dose-dependent manner (Figure 3A)."

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"Our findings provided direct evidence that DUb has the potential to be an effective therapeutic agent for CRC.To explore how DUb inhibits CRC cell proliferation, qRT-PCR analysis was performed to determine cancer-related genes using DUb-treated CRC cells."

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"We found that DUb inhibits CRC cell proliferation through up-regulating SIRT2 in CRC cells."

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"It was further demonstrated that DUb inhibited LoVo cell proliferation, migration and invasion by upregulating SIRT2."

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"In addition, DUb significantly decreased cell proliferation in tumor tissues as detected by immunohistochemical staining for Ki67 (Figure 1C), which can be used as an index of cell proliferation."

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ZUP1 activates cell population proliferation.

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ZUP1 activates cell population proliferation. 1 / 1

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"Dub in combination with X-linked inhibitor of Apoptosis (XIAP) and EDL-360 (a novel 1,2,3,4 -tetra hydro iso quinoline analog [ 14 ] remarkably attenuated glioma cell proliferation, invasion and metas[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

36208704

ZUP1 affects CRC growth

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ZUP1 inhibits CRC growth. 6 / 6

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"Scale bar = 50 µm in C. **p < 0.01.FIGURE 2: DUb inhibits the CRC growth in the patient-derived xenograft (PDX) tumor models."

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"These results shows that DUb reduces the migratory and invasive abilities of CRC cell.To determine the mechanism of DUb inhibiting CRC growth, a qRT-PCR array was performed."

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"Our results show that DUb markedly suppresses CRC growth in xenograft mouse models with colorectal carcinoma CT26 cells and human-derived xenograft tumor."

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"Therefore, DUb may inhibit CRC growth by affecting the metabolism of colorectal cancer.Taken together, as an important finding of the current study, DUb is determined to suppress CRC growth in xenograft mouse models with CRC CT26 cells and human CRC tissues."

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"Our results in this study demonstrates that DUb may be the potential agent for the treatment of CRC through upregulating SIRT2.FIGURE 1: DUb inhibits CRC growth in CT26 xenograft tumor models."

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"DUb was found to significantly attenuate CRC growth (Figure 1A) and weight (Figure 1B)."

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ZUP1 affects Ubiquitin

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ZUP1 binds Ubiquitin.

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ZUP1 binds Ubiquitin. 2 / 2

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"We surmised that this configuration might impart preferential binding of ZUFSP to Ub chains, as reported for other tandem UBDs ( Husnjak and Dikic, 2012; Sims and Cohen, 2009 )."

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"Pull-down experiments in lysate using Ub-, SUMO2-, or UFM1 conjugated agarose revealed that FLAG-ZUFSP and FLAG-ZUFSP (298-578) interacted with Ub-agarose, but not with UFM1- or SUMO2-agarose, suggesting that ZUFSP binds preferentially to Ub."

29563501

ZUP1 activates Ubiquitin.

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ZUP1 activates Ubiquitin. 2 / 2

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"In line with the UBZ/MIU-mediated selective binding of ZUFSP to K63-linked Ub chains and the key role of this modification in promoting protein recruitment to DNA damage sites ( Jackson and Durocher, [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"POH1 and USP5 possibly acting in concert is one example, while the K63-specific activity of ZUP1 may potentially allow Ub chain editing by neighbouring E3 ligases and/or regulated extraction of target proteins from chromatin via VCP/p97."

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ZUP1 inhibits Ubiquitin.

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ZUP1 inhibits Ubiquitin. 1 / 1

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"USP44, USP17L2/DUB3, USP11, ZUFSP and POH1 loss promotes the excessive spreading of Ub at DSBs and concomitant excessive accumulation of 53BP1 [57–65]."

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FLCN affects ZUP1

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FLCN activates ZUP1.

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FLCN activates ZUP1. 2 / 2

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"Birt–Hogg–Dubé (BHD) syndrome is caused by mutation of the folliculin (FLCN) gene that encodes the tumor suppressor protein folliculin [1]."

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"Loss‐of‐function pathogenic variants of FLCN are known to cause Birt‐Hogg‐Dubé (BHD) syndrome, an autosomal dominant disorder characterized by pulmonary and renal cysts, spontaneous pneumothorax, benign cutaneous fibrofolliculomas, and an increased risk of developing kidney tumors (Schmidt & Linehan, 2015)."

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Mutated FLCN activates ZUP1. 2 / 2

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"Birt–Hogg–Dubé (BHD) syndrome is caused by mutation of the folliculin (FLCN) gene that encodes the tumor suppressor protein folliculin [1]."

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"Inactive mutation of FLCN causes Birt–Hogg–Dubé syndrome (BHD, OMIM#135150)."

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FLCN inhibits ZUP1.

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FLCN inhibits ZUP1. 1 / 1

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"Loss of the tumor suppressor folliculin (FLCN) leads to Birt–Hogg–Dubé (BHD) syndrome, a condition characterized by cutaneous fibrofolliculomas, spontaneous pneumothorax and pulmonary cyst development, as well as renal cell carcinoma ."

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ZUP1

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ZUP1 translocates to the nucleus. 3 / 3

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"This finding revealed that ZUFSP could be translocated into the nucleus in response to RNA virus stimulation, highlighting its potential role in orchestrating anti-RNA virus immunity ( Fig. 4 E)."

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"Interestingly, VSV infection resulted in a striking and rapid translocation of ZUFSP into the nucleus, which could be observed as early as 1 h post-infection ( Fig. 4 F), reflecting the prompt respons[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"The mechanism that MAVS promote ZUFSP translocate to the nucleus remains unknown and needs to be further explored."

38460305

ZUP1 translocates from the cytoplasm to the nucleus. 1 / 1

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"Once MAVS is activated, ZUFSP can be translocated from the cytoplasm to the nucleus, where ZUFSP interacts with SMARCA5 to enhance the transcription of ISGs against RNA viruses ( Fig. 6 )."

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ZUP1 affects Neoplasm Invasiveness

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ZUP1 inhibits Neoplasm Invasiveness.

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ZUP1 inhibits Neoplasm Invasiveness. 3 / 3

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"It was further demonstrated that DUb inhibited LoVo cell proliferation, migration and invasion by upregulating SIRT2."

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"We found that DUb significantly inhibited the CRC cell migration and invasion inside Boyden Chambers in a dose-dependent manner (Figure 4)."

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ZUP1 activates Neoplasm Invasiveness.

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ZUP1 activates Neoplasm Invasiveness. 1 / 1

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"CircRNA ZUFSP promotes trophoblasts migration and invasion in vitro through sponging miR-203 to affect STOX1 expression [ 95 ]."

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ZUP1 affects CRC

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ZUP1 inhibits CRC. 4 / 4

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"These results show that DUb reduces CRC growth by suppressing tumor cell proliferation in vivo.To explore the effect of DUb on CRC cell proliferation, HCT116 and LoVo cells were treated with different concentrations of DUb for 96 h, and the cell viability was detected by the MTT assay."

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"In the present study, we reported that DUb inhibited CRC growth by upregulating SIRT2."

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"These data suggest that DUb inhibits CRC growth by impeding tumor cell proliferation.Given that DUb suppresses mouse-derived CRC growth, we speculated that DUb can also inhibit human-derived CRC growth by attenuating tumor cell proliferation."

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ZUP1 affects MAVS

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ZUP1 binds MAVS.

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ZUP1 binds MAVS. 2 / 2

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"Then, we conducted co-immunoprecipitation assay (Co-IP) to confirm the interaction between ZUFSP and MAVS in HEK293T cells ( Fig. 1 B)."

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"Mechanistically, ZUP1 binds to MAVS through its C-terminal domain independently of DUB activity."

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ZUP1 activates MAVS.

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ZUP1 activates MAVS. 1 / 1

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"We found that the overexpression of RIG-I, MDA5, MAVS or TBK1 could induce IFN-β promoter–driven luciferase activity, and that overexpression of ZUFSP further enhanced this effect of RIG-I, MDA5 and M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

38460305

ZUP1 affects IMD

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ZUP1 activates IMD.

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ZUP1 activates IMD. 2 / 2

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"A typical OTU Dub dTrabid rely on the OTU domain to inhibit the K63-linked ubiquitination of dTak1 and thus negatively regulate the IMD pathway (Hua et al., 2022)."

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"The above results suggested that the Dub CG4968 positively regulated the IMD pathway in S2 cells."

36778143

ZUP1 inhibits IMD.

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ZUP1 inhibits IMD. 1 / 1

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"Hua et al. provided a more mechanistic insight how the IMD pathway is negatively regulated by the deubiquitinase (Dub) dTrbd which targets K63-linked ubiquitination of dTak1."

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ZUP1 affects IL2RA

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ZUP1 activates IL2RA. 3 / 3

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"Dub induces CD25 + Foxp3 + Tregs via enhancement of IDO1-mediated Trp metabolism in colonic dendritic cells."

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"Dub prevents DSS-induced immunopathology by rebalancing CD25 + Foxp3 + Treg and IL17 + CD4 + T cell responses in the gut microenvironment."

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"The human homolog of Dub, C. innocuum, produces L-Lys and promotes CD25 + Foxp3 + Tregs to alleviate DSS-induced injury by upregulating IDO1 expression in DCs."

38351003

ZUP1 affects IFNB1

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ZUP1 inhibits IFNB1.

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ZUP1 inhibits IFNB1. 1 / 1

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"Lack of ZUFSP apparently inhibited the IFNB1 mRNA production after RNA virus but not DNA virus infection in THP1 and HeLa cells ( Fig. 2 B, C)."

38460305

ZUP1 increases the amount of IFNB1.

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ZUP1 increases the amount of IFNB1. 1 / 1

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"Also, the enhancement of IFNB1 expression induced by ZUFSP was impaired when SMARCA5 was knocked out ( Fig. 5 H), implying that SMARCA5 functioned downstream of ZUFSP."

38460305

ZUP1 activates IFNB1.

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ZUP1 activates IFNB1. 1 / 1

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"Further experimental results illustrated that NLS deletion of ZUFSP abolished the enhancement of IFNB1 promoter–driven luciferase activity induced by MAVS ( Fig. 4 D and Supplementary Table S2 ), indi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

38460305

ZUP1 affects DNA Damage

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ZUP1 inhibits DNA Damage.

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ZUP1 inhibits DNA Damage. 2 / 2

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"Furthermore, loss of ZUFSP in human cancer cells led to increased endogenous DNA damage in these cells, and we could further show that this endogenous DNA damage originates in S-phase."

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"While WT ZUFSP effectively rescued the DNA damage phenotype in ZUFSP depleted cells, the C360S mutant was unable to, suggesting that the DUB activity of ZUFSP is required to prevent spontaneous DNA damage in cells (XREF_FIG F)."

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ZUP1 activates DNA Damage.

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ZUP1 activates DNA Damage. 1 / 1

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29576527

ZUP1 affects Colitis

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ZUP1 activates Colitis. 3 / 3

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"Indeed, Dub colonization exerted an even greater protective effect against DSS-induced colitis than Akk, with lower body weight loss (Supplementary Fig. 2c), longer colon length (Fig. 2a and Supplementary Fig. 2d), lower histopathological score (Fig. 2b, c) and less colonic macrophage infiltration (Fig. 2c)."

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"Treg depletion in Foxp3-DTR mice reversed the differences observed between Veh-treated (WT-Veh) and Dub-colonized WT mice (WT-Dub) in disease phenotypes (Fig. 3d and Supplementary Fig. 4j), histological evaluation (Fig. 3e, f) and proinflammatory cytokine expression (Supplementary Fig. 4k), indicating that Foxp3 Tregs are essential for the Dub protective effect against DSS-induced colitis."

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"Lastly, we are making great efforts to generate a mutant Dub strain with dapF (encodes essential diaminopimelate epimerase for Lys biosynthesis) depletion, which will enable us to mechanistically dissect the specific role of Dub-synthesized Lys for mitigating DSS-induced colitis.In summary, our comprehensive analysis demonstrates a previously unrevealed role of Dub in activating the AhR-IDO1-Kyn circuit in DCs, which improved the unbalanced Treg/Th17 response to ameliorate both mucosal and systemic inflammation in DSS-induced colitis."

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MAVS affects ZUP1

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MAVS binds ZUP1.

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ZUP1 binds MAVS. 2 / 2

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"Then, we conducted co-immunoprecipitation assay (Co-IP) to confirm the interaction between ZUFSP and MAVS in HEK293T cells ( Fig. 1 B)."

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"Mechanistically, ZUP1 binds to MAVS through its C-terminal domain independently of DUB activity."

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MAVS activates ZUP1.

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MAVS activates ZUP1. 1 / 1

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"In summary, after RNA virus infection, MAVS promoted the transfer of ZUFSP from the cytoplasm to the nucleus for antiviral response.In order to understand how ZUFSP functions in the nucleus, proximity[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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Condensation affects ZUP1

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Condensation activates ZUP1. 2 / 2

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"However, dTrbd condensation is surprisingly dependent on the Linker region lacking LC properties, implying diverse regulatory mechanisms by which protein condensation mediates the Dub enzymatical processivity and the related biological processes."

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"We and colleagues recently found that In Drosophila, protein condensation and phase separation can mediate the Dub enzymatical activity of Otu to negatively contribute to the IMD innate immune pathway via the low-complexity (LC) domain (33)."

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ZUP1 affects metabolic process

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ZUP1 inhibits metabolic process.

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ZUP1 inhibits metabolic process. 1 / 1

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ZUP1 activates metabolic process.

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ZUP1 activates metabolic process. 1 / 1

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"Altogether, we demonstrated that Trp metabolism in DCs was promoted by Dub, and the resulting Kyn enhancement from strengthened IDO1 expression rebalanced Treg/Th17 responses and ultimately exerted a protective effect on DSS-induced colitis."

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ZUP1 affects cell death

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ZUP1 inhibits cell death.

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ZUP1 inhibits cell death. 1 / 1

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"A report uncovered that the combination treatment of EDL-360 with DUb effectively suppressed glioma tumorigenesis by inducing cell death (Hosni-Ahmed et al., 2014)."

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ZUP1 activates cell death.

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ZUP1 activates cell death. 1 / 1

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"DUb was also found to induce PTP-dependent cell death in Clone-9 and MH1C1 cells (Devun et al., 2010)."

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ZUP1 affects RPA

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ZUP1 ubiquitinates RPA.

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ZUP1 leads to the ubiquitination of RPA. 1 / 1

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"ZUFSP interacts with and modulates ubiquitination of the replication protein A (RPA) complex."

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ZUP1 binds RPA.

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ZUP1 binds RPA. 1 / 1

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"The DNA dependent binding of ZUFSP to the RPA complex was confirmed by co-immunoprecipitation experiments."

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ZUP1 affects PTEN

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ZUP1 decreases the amount of PTEN.

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ZUP1 decreases the amount of PTEN. 1 / 1

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"The results showed that the addition of USP10 significantly reduced the ubiquitination levels of PTEN in vitro (Fig. 1G), further suggesting USP10 was a Dub of PTEN."

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ZUP1 activates PTEN.

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ZUP1 activates PTEN. 1 / 1

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"To the best of our knowledge, this is the first study on a Dub that activates PTEN by preventing its K63-linked polyubiquitination.Ubiquitination is a critical post-translational modification that regulates protein stability, subcellular localization, and activity."

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ZUP1 affects Neoplasms

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ZUP1 inhibits Neoplasms. 2 / 2

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"Compared with DMSO group, DUb significantly decreased tumor volume (Figure 2A) of human-derived xenograft tumor."

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"Our results in this study demonstrates that DUb may be the potential agent for the treatment of CRC through upregulating SIRT2.FIGURE 1: DUb inhibits CRC growth in CT26 xenograft tumor models."

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ZUP1 affects Neoplasm Metastasis

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ZUP1 inhibits Neoplasm Metastasis. 2 / 2

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"In addition, DUb inhibits breast cancer cell growth and metastasis by regulating the ROS/p53/BAI1 pathway (Cao et al., 2020)."

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ZUP1 affects Glioma

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ZUP1 inhibits Glioma. 2 / 2

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"A report uncovered that the combination treatment of EDL-360 with DUb effectively suppressed glioma tumorigenesis by inducing cell death (Hosni-Ahmed et al., 2014)."

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"Recent study demonstrated that DUb combined with either EDL-360 or thialysine significantly inhibited the growth of human acute leukemia or glioma (Hosni-Ahmed et al., 2014)."

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Ubiquitin affects ZUP1

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ZUP1 binds Ubiquitin. 2 / 2

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"We surmised that this configuration might impart preferential binding of ZUFSP to Ub chains, as reported for other tandem UBDs ( Husnjak and Dikic, 2012; Sims and Cohen, 2009 )."

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UBE2N affects ZUP1

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UBE2N inhibits ZUP1. 2 / 2

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"KD of UBC13 strongly decreases ZUFSP accumulation at damage sites further supporting the role of K63 linked ubiquitin chains in this process [XREF_BIBR]."

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SMARCA5 affects ZUP1

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SMARCA5 binds ZUP1.

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ZUP1 binds SMARCA5. 1 / 1

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"The interaction between ZUFSP and SMARCA5 was increased after VSV infection ( Fig. 5 D), strengthening the evidence of their functional collaboration in response to virus infection."

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SMARCA5 activates ZUP1.

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SMARCA5 activates ZUP1. 1 / 1

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"Moreover, we conducted ChIP-qPCR with Flag-ZUFSP and H3K27ac antibodies in sgCTRL and SMARCA5 knock-out HEK293T cells ( Fig. 5 J, K) and found SMARCA5 deficiency decreased the occupancy of Flag-ZUFSP [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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RpsN1 affects ZUP1

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RpsN1 activates ZUP1. 1 / 1

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"We incubated Otu FL with gradated amounts of Bam and found that addition of Bam, but not BSA, significantly increased the Dub activity of Otu FL ( Figures 5 J and S5 J)."

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Propensity be connected consistency survey findings globe affects ZUP1

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Propensity be connected consistency survey findings globe activates ZUP1. 1 / 1

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"So connected is this generation that their propensity to be digitally connected to one another and the consistency of the survey findings across the globe led Barna ( 2019 ) to dub today 's 18 to 35-year-olds the `` connected generation '' ."

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PolyUb affects ZUP1

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ZUP1 binds polyUb. 1 / 1

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"Having identified a UBZ domain in ZUFSP that binds polyUb, we asked whether the addition of this domain would confer DUB activity to the catalytic domain of ZUFSP."

29576527

Calcium(2+) affects ZUP1

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Calcium(2+) inhibits ZUP1. 1 / 1

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"By employing extracellular and/or intracellular calcium chelating compounds, it is possible to infect cells in Ca depleted conditions and probe resulting infectivity (Dubé et al., 2016)."

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ZUP1 affects video

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ZUP1 activates video. 1 / 1

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"Virtual Dub converts H264 AVI (compressed) to ImageJ supported video format (raw AVI) (uncompressed)."

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ZUP1 affects ubH2B nucleosomes

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ZUP1 deubiquitinates ubH2B nucleosomes. 1 / 1

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"The in vitro de-ubiquitination assay showed that the ubH2B nucleosomes were successfully de-ubiquitinated by wild-type DUB (DUB-wt) in vitro with high efficiency, but not by its catalytically inactive DUB mutant (DUB-mut), as examined by western blot experiments (Fig. 2d)."

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ZUP1 affects supercomplexes

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ZUP1 activates supercomplexes. 1 / 1

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"They concluded that DUb can enhance the activities of supercomplexes such as I / III and II / III but is not determinant in the activities of the individual complexes , I , III , and IV , or in the rate of oxygen consumption , which was completely unaffected by DUb ."

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ZUP1 affects replication protein

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ZUP1 leads to the ubiquitination of replication protein. 1 / 1

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"ZUFSP interacts with and modulates ubiquitination of the replication protein A (RPA) complex."

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ZUP1 affects reactive oxygen species

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ZUP1 inhibits reactive oxygen species. 1 / 1

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"In fibroblasts derived from Leber Hereditary Optic Neuropathy ( LHON ) harboring the m.11778G > A mutation , DUb highly decreases reactive oxygen species ( ROS ) from affected and control cells [ 102 ] ."

33557229

ZUP1 affects polyUb

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ZUP1 binds polyUb. 1 / 1

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"Having identified a UBZ domain in ZUFSP that binds polyUb, we asked whether the addition of this domain would confer DUB activity to the catalytic domain of ZUFSP."

29576527

ZUP1 affects laccases

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ZUP1 activates laccases. 1 / 1

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"The lack of activity against textile azo dyes was also reported for laccases produced by Pycnoporus cinnabarinus ( Camarero et al., 2005 ), Sclerotium rolfsii ( Campos et al., 2001 ) and S. coelicolor[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

20018508

ZUP1 affects inflammatory response

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ZUP1 inhibits inflammatory response. 1 / 1

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"Our findings that Dub relieves host mucosal and extraintestinal inflammation by rebalancing Treg/Th17 responses are perfectly in line with 2 landmark studies that demonstrate acetate and propionate regulate GPR43-dependent immunosuppression by cTregs under DSS or T cell-transfer conditions ."

38351003

ZUP1 affects immediate respiration concomitant membrane potential

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ZUP1 inhibits immediate respiration concomitant membrane potential. 1 / 1

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"DUb caused an immediate increase in respiration and concomitant decline in membrane potential , suggesting opening of a leak channel in both groups ( Figure 3d ) ."

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ZUP1 affects homogeneity

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ZUP1 activates homogeneity. 1 / 1

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"In fact , prevention of the current leakage was found to improve the temperature homogeneity over the welding area by Dub e et al. [ 72 ] ."

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ZUP1 affects dioxygen

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ZUP1 activates dioxygen. 1 / 1

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"Furthermore, DUb restored Fmr1 KO rates of oxygen consumption to control values, suggesting increased substrate oxidation ( Fig. 8 )."

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ZUP1 affects cell migration

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ZUP1 inhibits cell migration. 1 / 1

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"We found that DUb significantly inhibited the CRC cell migration and invasion inside Boyden Chambers in a dose-dependent manner (Figure 4)."

35177983

ZUP1 affects cell growth

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ZUP1 inhibits cell growth. 1 / 1

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"In addition, DUb inhibits breast cancer cell growth and metastasis by regulating the ROS/p53/BAI1 pathway (Cao et al., 2020)."

35177983

ZUP1 affects bacterial laccases

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ZUP1 activates bacterial laccases. 1 / 1

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"Recently, bacterial laccases produced by Streptomyces ( Dubé et al., 2008; Molina-Guijarro et al., 2009 ) and Bacillus ( Pereira et al., 2009 ) have been found to be useful for the decolouration of in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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ZUP1 affects autophagy of mitochondrion

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ZUP1 activates autophagy of mitochondrion. 1 / 1

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"In contrast, Dub*-TRIM5α, which is a surrogate for wild type TRIM5α because it has intact ubiquitination function, potently restored mitophagy in these experiments (Figure 3K)."

37905089

ZUP1 affects apoptotic process

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ZUP1 inhibits apoptotic process. 1 / 1

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"Therefore, 16 potential radiotherapy-resistant genes were here screened using the human genome-wide CRISPRa technology, and further experiments were carried out to confirm that the high expression of MTFR1, NIPSNAP1, ZUP1, PSMB2, PSMA6, EEF1A1, TMSB4Y and TAF1L can reduce the apoptosis of lymphoma cells and increase the probability of radiotherapy resistance."

36743888

ZUP1 affects angiogenesis

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ZUP1 inhibits angiogenesis. 1 / 1

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"DUb suppresses angiogenesis via the ROS / p53 / BAI1 signaling pathway in vascular endothelial cells [ 105 ] ."

33557229

ZUP1 affects activities of supercomplexes

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ZUP1 activates activities of supercomplexes. 1 / 1

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"The precise mechanism by which DUb increases the activities of supercomplexes [ 100 ] remains unknown ."

33557229

ZUP1 affects Weight Loss

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ZUP1 activates Weight Loss. 1 / 1

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"Furthermore, Dubé et al. showed that dihydroceramides increased with both diet-induced weight loss and exercise training, whereas sphingosines and total ceramide species were decreased with exercise, [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

22120643

ZUP1 affects UBD

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ZUP1 activates UBD. 1 / 1

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"Also, the ZUFSP helical arm (ZHA) domain orient in such a way that it moves closer to the Ub, this orientation enables the formation of a UBD which is very peculiar to ZUFSP."

33538664

ZUP1 affects TP53BP1

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ZUP1 inhibits TP53BP1. 1 / 1

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"USP44, USP17L2/DUB3, USP11, ZUFSP and POH1 loss promotes the excessive spreading of Ub at DSBs and concomitant excessive accumulation of 53BP1 [57–65]."

31769469

ZUP1 affects SRGN

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ZUP1 binds SRGN. 1 / 1

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"The final data collection and refinement statistics for the ZUFSP-Ub and Prg complex structure is shown in XREF_TABLE."

29576527

ZUP1 affects SMARCA5

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ZUP1 binds SMARCA5. 1 / 1

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"The interaction between ZUFSP and SMARCA5 was increased after VSV infection ( Fig. 5 D), strengthening the evidence of their functional collaboration in response to virus infection."

38460305

ZUP1 affects RPA1

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ZUP1 leads to the ubiquitination of RPA1. 1 / 1

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"More specifically, ZUFSP RNAi enhanced RPA1 ubiquitination after HU treatment, whereas RPA2 ubiquitination was enhanced under basal conditions."

29563501

ZUP1 affects ROCK2

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ZUP1 inhibits ROCK2. 1 / 1

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"Considering Dub mediated attenuation of ROCKII, the key player for metastatic tumor formation, experiment was directed to investigate the accountability of ROCKII by in vitro assay."

36208704

ZUP1 affects RIGI

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ZUP1 activates RIGI. 1 / 1

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38460305

ZUP1 affects QPCT

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ZUP1 activates QPCT. 1 / 1

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"Because other DUbs that promote QC such as Rpn11 , Doa4 and Ubp14 ( Fig 1A ) are also required for degrading folded proteins through non-QC pathways [ S3D Fig in S1 File and 20 , 22 ] , we examined the role of Ubp6 in the degradation of two folded proteins , Stp1 and Deg1-Ura3 ."

32401766

ZUP1 affects Pain, Postoperative

| 1

ZUP1 activates Pain, Postoperative. 1 / 1

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") injection of A-317567 efficaciously exerted an analgesic effect on complete Freud’s adjuvant-induced inflammatory thermal hyperalgesia and postoperative pain caused by skin incision (Dubé et al., 2005)."

33192609

ZUP1 affects Obesity

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ZUP1 activates Obesity. 1 / 1

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22120643

ZUP1 affects OTUD5

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ZUP1 activates OTUD5. 1 / 1

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"In this study, we identified an elegant mechanism to counteract deubiquitylation that allows a DUB, OTUD5, to be efficiently targeted for degradation."

40128189

ZUP1 affects Melanoma

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ZUP1 activates Melanoma. 1 / 1

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"Taken together, the data in this study demonstrated, it was considered that Dub arrested subcutaneously injected B16F10 induced pulmonary metastatic melanoma by calibrating ROS-ROCKII- Cofilin axis as[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

36208704

ZUP1 affects Lymphoma

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ZUP1 inhibits Lymphoma. 1 / 1

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36743888

ZUP1 affects Leukemia

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ZUP1 inhibits Leukemia. 1 / 1

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"Recent study demonstrated that DUb combined with either EDL-360 or thialysine significantly inhibited the growth of human acute leukemia or glioma (Hosni-Ahmed et al., 2014)."

35177983

ZUP1 affects L-lysine zwitterion

| 1

ZUP1 activates L-lysine zwitterion. 1 / 1

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"The human homolog of Dub, C. innocuum, produces L-Lys and promotes CD25 + Foxp3 + Tregs to alleviate DSS-induced injury by upregulating IDO1 expression in DCs."

38351003

ZUP1 affects Kyn

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ZUP1 activates Kyn. 1 / 1

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"In agreement with these results, Dub colonization led to a significantly increased serum Kyn concentration (Fig. 4i) at early phases of colitis progression (D0–D3)."

38351003

ZUP1 affects Infections

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ZUP1 activates Infections. 1 / 1

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"Lastly, Dubé et al. reported increased susceptibility to Mtb infection in Mincle KO mice at late stages of infection ."

38844494

ZUP1 affects Imd

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ZUP1 inhibits Imd. 1 / 1

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"Dub Ubiquitin-specific protease 36 (dUSP36) can interact with Imd and negatively regulate the Imd pathway by inhibiting K63-linked ubiquitination of Imd (Thevenon et al., 2020)."

36778143

ZUP1 affects INS

| 1

ZUP1 inhibits INS. 1 / 1

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"Moreover, Dubé et al. (2011) found that while exercise-induced weight loss improved insulin sensitivity concomitant with a reduction in ceramide, diet-induced weight loss negated insulin resistance wi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

22560211

ZUP1 affects IL17A

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ZUP1 activates IL17A. 1 / 1

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"Dub prevents DSS-induced immunopathology by rebalancing CD25 + Foxp3 + Treg and IL17 + CD4 + T cell responses in the gut microenvironment."

38351003

ZUP1 affects IFIH1

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ZUP1 activates IFIH1. 1 / 1

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38460305

ZUP1 affects IDO1

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ZUP1 increases the amount of IDO1. 1 / 1

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"The Dub-derived metabolite L-Lys upregulates AhR-dependent IDO1 expression in colonic DCs."

38351003

ZUP1 affects H3K27Ac

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ZUP1 activates H3K27Ac. 1 / 1

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"ChIP-qPCR showed that ZUFSP deficiency decreased the occupancy of H3K27ac at ISGs, which meant reduced chromatin accessibility ( Fig. 5 I)."

38460305

ZUP1 affects H2AX

| 1

ZUP1 deubiquitinates H2AX. 1 / 1

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"Inhibition of Dub activity by N-ethylmaleimide (NEM) prevented deubiquitination of H2AX by Dub3 and to support the specificity of the assay, we demonstrate that USP28, an aspecific Dub, previously rep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

24704006

ZUP1 affects Fmr1 KO

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ZUP1 activates Fmr1 KO. 1 / 1

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"Furthermore, DUb restored Fmr1 KO rates of oxygen consumption to control values, suggesting increased substrate oxidation ( Fig. 8 )."

35500860

ZUP1 affects FOXP3

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ZUP1 activates FOXP3. 1 / 1

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"Dub prevents DSS-induced immunopathology by rebalancing CD25 + Foxp3 + Treg and IL17 + CD4 + T cell responses in the gut microenvironment."

38351003

ZUP1 affects ELAVL1

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ZUP1 activates ELAVL1. 1 / 1

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"A typical OTU Dub dTrabid rely on the OTU domain to inhibit the K63-linked ubiquitination of dTak1 and thus negatively regulate the IMD pathway (Hua et al., 2022)."

36778143

ZUP1 affects E3_Ub_ligase

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ZUP1 activates E3_Ub_ligase. 1 / 1

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38572758

ZUP1 affects Deubiquitinase

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ZUP1 activates Deubiquitinase. 1 / 1

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"In this study, we identified an elegant mechanism to counteract deubiquitylation that allows a DUB, OTUD5, to be efficiently targeted for degradation."

40128189

ZUP1 affects DNA-templated transcription

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ZUP1 activates DNA-templated transcription. 1 / 1

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"Once MAVS is activated, ZUFSP can be translocated from the cytoplasm to the nucleus, where ZUFSP interacts with SMARCA5 to enhance the transcription of ISGs against RNA viruses ( Fig. 6 )."

38460305

ZUP1 affects DNA replication

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ZUP1 inhibits DNA replication. 1 / 1

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"Given that loss of ZUFSP in human cancer cells both causes endogenous DNA replication stress and sensitizes cells to further exogenous DNA damage, it will now be important to understand whether, and how, ZUFSP loss synergizes with defects in other DDR pathways."

29576527

ZUP1 affects DDX53

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ZUP1 inhibits DDX53. 1 / 1

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"Our results in this study demonstrates that DUb may be the potential agent for the treatment of CRC through upregulating SIRT2.FIGURE 1: DUb inhibits CRC growth in CT26 xenograft tumor models."

35177983

ZUP1 affects Cysts

| 1

ZUP1 activates Cysts. 1 / 1

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"Birt–Hogg–Dubé syndrome (BHD) is a recently described cause of multiple pulmonary cysts and constitutes one of the three most important causes of cystic lung disease with LAM and PLCH."

32943413

ZUP1 affects Cys223

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BHPTP binds ZUP1 and Cys223. 1 / 1

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"PRED of the binding between BHPTP and ZUFSP revealed Cys223, Arg408, Met410, Asn460, and Tyr465 as the crucial residues responsible for this interaction."

32744966

ZUP1 affects ChFP

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ZUP1 activates ChFP. 1 / 1

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"As expected, expression of either Dub along with the poly(A)-reporter in parental HCT116 cells markedly increased the ChFP:GFP ratio of the stall reporter (Figure 4A,B)."

32011234

ZUP1 affects Carcinogenesis

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ZUP1 inhibits Carcinogenesis. 1 / 1

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"A report uncovered that the combination treatment of EDL-360 with DUb effectively suppressed glioma tumorigenesis by inducing cell death (Hosni-Ahmed et al., 2014)."

35177983

ZUP1 affects CD25 Foxp3

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ZUP1 activates CD25 Foxp3. 1 / 1

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"Dub or Akk colonization significantly increased CD25 Foxp3 Tregs and markedly decreased IL17 CD4 T cells in the MLNs and cLP (Fig. 3b, c), but only mildly reduced IFNγ CD4 T cells in the cLP (Supplementary Fig. 4c) compared to EF or Veh controls, with or without gut microbiota at D7."

38351003

ZUP1 affects BHPTP

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BHPTP binds ZUP1 and Cys223. 1 / 1

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"PRED of the binding between BHPTP and ZUFSP revealed Cys223, Arg408, Met410, Asn460, and Tyr465 as the crucial residues responsible for this interaction."

32744966

ZUP1 affects AhR-IDO1-Kyn circuit

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ZUP1 activates AhR-IDO1-Kyn circuit. 1 / 1

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38351003

ZUP1 affects ABCC8

| 1

ZUP1 inhibits ABCC8. 1 / 1

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"Similar to the Neo controls, Abx-Dub had markedly increased expression of ZO-1, OCLN and Muc2 in the cIECs (Fig. 2h) and significantly reduced of Il1b, Il6, and Tnfa in the cLP (Fig. 2i) compared with Abx-Akk, Abx-EF, Abx or Veh groups.In order to better define the protective component of Dub, Abx mice were gavaged with filtered Dub supernatant (Dub.sup) or heat-inactivated (HI) Dub and then subjected to DSS treatment (Supplementary Fig. 3h)."

38351003

TP53BP1 affects ZUP1

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TP53BP1 activates ZUP1. 1 / 1

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"Using cyclin A as a marker for S/G 2 -phase and 53BP1 as a DNA damage marker, we observed that both G 1 - and S/G 2 -phase cells displays increased 53BP1 signal in ZUFSP depleted cells, indicative of increased DNA breaks in both these cell-cycle phases (XREF_FIG D)."

29576527

SRGN affects ZUP1

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ZUP1 binds SRGN. 1 / 1

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"The final data collection and refinement statistics for the ZUFSP-Ub and Prg complex structure is shown in XREF_TABLE."

29576527

Reactive Oxygen Species affects ZUP1

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Reactive Oxygen Species activates ZUP1. 1 / 1

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"Confocal microscopy further explained the situation by demonstrating reduced E -Cadherin expression with somehow maintenance of intracellular ROS as DCF fluorescence in ROCKII siRNA transfection again[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

36208704

RPA affects ZUP1

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ZUP1 binds RPA. 1 / 1

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"The DNA dependent binding of ZUFSP to the RPA complex was confirmed by co-immunoprecipitation experiments."

29476094

RNA Virus Infections affects ZUP1

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RNA Virus Infections increases the amount of ZUP1. 1 / 1

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"The production of type I IFN and ISGs was impaired in ZUFSP-deficient cells, while the overexpression of ZUFSP promoted the induction of type I IFN by RNA virus infection."

38460305

PTPN1 affects ZUP1

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PTPN1 activates ZUP1. 1 / 1

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"High PTP1B expression can increase Ras activity, thereby increasing the risk of tumorigenesis (Dubé et al., 2004)."

35059399

PNPLA2 affects ZUP1

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PNPLA2 activates ZUP1. 1 / 1

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"Adipose triglyceride lipase (ATGL) deletion from adipocytes impairs lipolysis and reduces peripheral energy source availability ( Dubé et al., 2015 )."

29241541

OTUD5 affects ZUP1

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OTUD5 activates ZUP1. 1 / 1

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"Thus, TRIP12-generated K29-linkage besides protecting itself from OTUD5-mediated deubiquitylation, also 1) facilitates UBR5-dependent assembly of K48-linked chains and 2) modestly protects K48-linkages from disassembly as forms of mixed/branched linkages."

40128189

NFE2L2 affects ZUP1

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NFE2L2 inhibits ZUP1. 1 / 1

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"Together, these results suggest that the SREBPs activate not only cholesterol and lipid biosynthesis, but an important component of the NRF2 anti-oxidant response, both of which are inhibited by TRC8.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

22939900

Mice affects ZUP1

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Mice activates ZUP1. 1 / 1

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"Additionally, WT mice gavaged with Dub for 5 consecutive days starting from day 3 post-DSS administration (Supplementary Fig. 2j) exhibited longer colon length (Supplementary Fig. 2k, l) and improved histopathology (Supplementary Fig. 2m, n) compared with mice receiving EF or untreated controls, suggesting that Dub also has therapeutic effects on DSS-induced colitis.To further evaluate the probiotic effect of Dub on colitis, we used the mouse model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis (Supplementary Fig. 3a)."

38351003

MEM affects ZUP1

| 1

MEM inhibits ZUP1. 1 / 1

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"Both MEM and CTX can inhibit PBPs and Ldts (Dubée et al., 2012) but only MEM can do so efficiently while being an extremely poor substrate to beta-lactamases (Tremblay et al., 2010)."

37009497

Genome polyprotein affects ZUP1

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Genome polyprotein activates ZUP1. 1 / 1

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29576528

GCG affects ZUP1

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GCG inhibits ZUP1. 1 / 1

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"GLP-2 acts on intestinal crypts to stimulate cell proliferation and inhibit apoptosis (Dubé et al., 2006)."

38725663

Cys223 affects ZUP1

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BHPTP binds ZUP1 and Cys223. 1 / 1

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"PRED of the binding between BHPTP and ZUFSP revealed Cys223, Arg408, Met410, Asn460, and Tyr465 as the crucial residues responsible for this interaction."

32744966

Cofilin affects ZUP1

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Cofilin activates ZUP1. 1 / 1

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36208704

Cardiomegaly affects ZUP1

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Cardiomegaly inhibits ZUP1. 1 / 1

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"On the other hand, chronic pulmonary congestion, septal thickening, and the compressive effects of cardiomegaly may reduce lung volumes ( Dubé et al., 2016 )."

29673610

CYP27A1 affects ZUP1

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CYP27A1 inhibits ZUP1. 1 / 1

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"Both MEM and CTX can inhibit PBPs and Ldts (Dubée et al., 2012) but only MEM can do so efficiently while being an extremely poor substrate to beta-lactamases (Tremblay et al., 2010)."

37009497

Bro1 affects ZUP1

| 1

Bro1 activates ZUP1. 1 / 1

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"Bro1 also stimulates the Dub activity of Doa4 by direct binding ( Richter et al., 2007 )."

19410548

BHPTP affects ZUP1

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BHPTP binds ZUP1 and Cys223. 1 / 1

| 1

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reach

"PRED of the binding between BHPTP and ZUFSP revealed Cys223, Arg408, Met410, Asn460, and Tyr465 as the crucial residues responsible for this interaction."

32744966

A-317567 affects ZUP1

| 1

A-317567 inhibits ZUP1. 1 / 1

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"In acutely dissociated adult rat DRG neurons, A-317567 was shown to produce a potent blocking of ASIC3 with an IC of 1.025 μM and to equipotently block the sustained phase of the ASIC3-like current, a biphasic current akin to that of cloned ASIC3 (Dubé et al., 2005)."

33192609

3-bromo-6-{[4-hydroxy-1-3 affects ZUP1

| 1

3-bromo-6-{[4-hydroxy-1-3 activates ZUP1. 1 / 1

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"3-bromo-6-{[4-hydroxy-1-3 (3-phenylbutanoyl) piperidin-4-yl] methyl}-4H,5H,6H,7H-thieno [2,3- C] pyridine-7-one (BHPTP) which is an inhibitor of USP7 was repurposed to target ZUFSP."

32744966

1,4-dithiothreitol affects ZUP1

| 1

1,4-dithiothreitol inhibits ZUP1. 1 / 1

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"Strikingly, we found that DTT treatments almost completely abolished Otu Dub activity ( Figure 1 H)."

30879902