IndraLab

Statements

reach
"Pull-down experiments in lysate using Ub-, SUMO2-, or UFM1 conjugated agarose revealed that FLAG-ZUFSP and FLAG-ZUFSP (298-578) interacted with Ub-agarose, but not with UFM1- or SUMO2-agarose, suggesting that ZUFSP binds preferentially to Ub."
29563501
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"20 Thus, probes containing short Ub peptides may not recapitulate the interaction between DUB and full length Ub."
| PMC
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"Interestingly, while Ub and ISG15 bound to CCHFV OTU in similar orientations (Fig. 7A and B), the β-grasp domains of these substrates were rotated ~ 75° with respect to the orientation that Ub binds to a representative eukaryotic OTU DUB from yeast, yOTU1 (Fig. 7A)."
28625850
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"This closely parallels the use of Ubal to obtain Ub-bound DUB structures, prior to the development of DUB ABPs."
28196299
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"Binding of Ub to the DUB would result in fluorescence, and conversely, no fluorescence would occur in the presence of an inhibitor (Ott et al., 2017)."
30851297