A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.

IndraLab

Statements

databases
phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
reading

CDK4 phosphorylates RB1. 9 / 532
1 | 1 18 230 270
reach
"As expected, the knockdown of cyclin D1 or CDK4 inhibited Rb phosphorylation."
sparser
"Upon phosphorylation of Rb1 by the cyclin-dependent kinases Cdk4 and Cdk6, the E2F transcription factors are released from Rb1 [32] ."
reach
"Therefore, it is conceivable to expect that HTLV-1 infection and Tax expression inactivates both p16ink4 and p15ink4 resulting in activation of Cdk4, which subsequently phosphorylates Rb protein to releases E2F."
sparser
"Cyclin D1 and CDK4 phosphorylate and inactivate Rb during the G1/S transition, thereby allowing cell cycle progression to occur [6] ."
rlimsp
"The data suggest that the redundancy in phosphorylation of RB by CDK2 and CDK4 in Nalm-6 extracts is limited."
sparser
"Rb is phosphorylated by CDK2, CDK4 and CDK6, which are inhibited by p16, p21 and p27 (CKI: cyclin-dependent kinase inhibitor)."
reach
"The first one refers to the extent to which Cdk4 contributes to the pseudo-hyper-phosphorylation of pRb, whereas the second one refers to the metabolic rate of the cell."
reach
"For this purpose, cyclinD1 and Cdk4 phosphorylates only RB, but not FOXM1c, so that cyclinD1 and Cdk4 increases the transcriptional activity of FOXM1c without phosphorylating FOXM1c and activates FOXM1c independently of cyclin and Cdk phosphorylation sites in FOXM1c."
sparser
"Finally, CDK4 and CDK6 may have different preferences for phosphorylation of specific sites in pRb protein [ xref ], and we have recently found distinct specificities of pRb phosphorylation by CDK4 activated by cyclin D1 or cyclin D3 [ xref ]."
CDK4 phosphorylates RB1 on S780. 9 / 24
1 1 4 | 1 11 5
reach
"We conclude that a genetic background deprived of decorin favors cell cycle progression of HCC by repressing AP4, inducing p21 Waf1 and Cip1, and enhancing phosphorylation of P-Rb at Ser780 by CDK4 and CyclinD complex."
sparser
"Phosphorylation of pRb by Cdk4-cyclin D at specific site Ser780 inactivates pRb and leads to release of E2F proteins from the inhibitory pRb-E2F-complex [ xref ]."
hprd
No evidence text available
biopax:phosphositeplus
No evidence text available
rlimsp
"Cdk4 specific phosphorylation of pRb on Ser780 and were normalized to total amount of pRb, β-actin served as internal control."
signor
"Cyclin d1 is known to activate cdk4, which then phosphorylates the rb protein, leading to cell cycle progression."
sparser
"Masatoshi et al. demonstrated that cyclin D-CDK4 complex is able to phosphorylate Rb at S780 in vivo and in vitro [ xref ]."
sparser
"We found that phorbol ester induces unusually early expression of cyclin D2 in B-1 but not B-2 cells, that this early-expressed cyclin D2 associates with Cdk4, and that this correlates with assembly of active kinase complexes and phosphorylation of Rb at the Cdk4 phosphoacceptor Ser 780 site."
hprd
No evidence text available
CDK4 phosphorylates RB1 on S795. 10 / 11
1 1 2 | 2 3 2
reach
"Notably both Cdk2 and Cdk4 kinase complexes phosphorylate, during the G 1 to S transition, pRb protein on Ser 795."
biopax:phosphositeplus
No evidence text available
reach
"Sequential phosphorylation of pRb by cyclin dependent kinases including CDK4 regulates S-phase of cells [XREF_BIBR], Cyclin D1 and CDK4 complex phosphorylates pRb at Ser 795 residue to induce DNA synthesis [XREF_BIBR], We directly determined the role of miR-21-regulated cyclin D1 in renal cancer cell proliferation."
signor
"Cyclin d1 is known to activate cdk4, which then phosphorylates the rb protein, leading to cell cycle progression."
sparser
"While Cdk4-cyclin D efficiently and specifically phosphorylates pRb on Ser780 and Ser795, Cdk2-cyclin A/E does not."
rlimsp
"The differential effects of phosphorylation on pRb function coincide with modification of distinct sets of sites. Serine 795 is phosphorylated efficiently by Cdk4, even in the absence of an intact LXCXE motif in cyclin D, but not by Cdk2 or Cdk3."
hprd
No evidence text available
rlimsp
"Phosphorylation of serines 608/612 inhibits binding of E2F-1 to RB. In Nalm-6 acute lymphoblastic leukemia extracts, serine 608 is phosphorylated by CDK4/6 complexes but not by CDK2. We reasoned that phosphorylation of serines 608/612 by redundant CDKs could accelerate phospho group formation and determined which G1 CDK contributes to serine 612 phosphorylation. Here, we report that CDK4 complexes from Nalm-6 extracts phosphorylated in vitro the CDK2-preferred serine 612, which was inhibited by p16INK4a, and fascaplysin. In contrast, serine 780 and serine 795 were efficiently phosphorylated by CDK4 but not by CDK2."
hprd
No evidence text available
sparser
"With CCND1 stimulation and CDKN2A inhibition, CDK4 phosphorylates the tumor suppressor retinoblastoma protein (RB) at serine780 and serine795 [ xref ], allowing pRB to activate the transcriptional factor E2F [ xref ]."
CDK4 phosphorylates RB1 on T826. 9 / 9
1 1 7 |
hprd
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
signor
"We demonstrate that phosphorylation by either cdk2-cyclin a, which phosphorylates t821, or cdk4-cyclin d1, which phosphorylates threonine 826, can disable prb for subsequent binding of an lxcxe protein."
hprd
No evidence text available
hprd
No evidence text available
biopax:phosphositeplus
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
Modified CDK4 leads to the phosphorylation of RB1. 7 / 7
| 7
reach
"At the cellular level, the TL mediated decrease in CDK4 protein levels in HeLa cells causes reduced phosphorylation of Rb resulting in cell cycle arrest at the G1 phase."
reach
"Specifically, increased expression of CDK4 due to elevations in copy number and through the activation of the mTOR pathway via PTEN and TSC2 mutations leads to phosphorylation of Rb in a majority of human PanNETs."
reach
"Frankincense essential oil suppressed cyclin D1 and cdk4 expression may lead to suppressed Rb phosphorylation which results in suppressed cell cycle progression in pancreatic cancer cells [XREF_BIBR]."
reach
"Over-expression of cyclin-D or CDK4 kinases from amplification, mutation, or chromosomal translocation can lead to enhanced Rb phosphorylation and poor prognosis."
reach
"RAS and CDK4 co-expression promotes Rb phosphorylation, ultimately resulting in human invasive neoplasm."
reach
"Higher levels of CDK4 may lead to RB phosphorylation impairing its function in cell cycle control [XREF_BIBR]."
reach
"Furthermore, ectopic expression of cyclin D1 but not CDK4, substantially increased RB phosphorylation and conferred substantial resistance to CDK4/6 inhibitors in SCCOHT-1 and BIN-67 cells."
CDK4 phosphorylates RB1 on S788. 4 / 4
1 3 |
biopax:phosphositeplus
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
Kinase-active CDK4 phosphorylates RB1 on serine. 4 / 4
2 2 |
bel
"\"progression through G1 phase of cell cycle requires phosphorylation of the retinoblastoma gene product (pRb) by the cyclin D-dependent serine-threonine kinases CDK4 and CDK6"
bel
"By inhibiting selectively either cdk4/6 or cdk2, we show that endogenous D-type cyclins, acting with cdk4/6, are able to phosphorylate pRb only partially, a process that is likely to be completed by cyclin E-cdk2 complexes. Furthermore, cyclin E-cdk2 is unable to phosphorylate pRb in the absence of prior phosphorylation by cyclin D-cdk4/6 complexes."
bel
"By inhibiting selectively either cdk4/6 or cdk2, we show that endogenous D-type cyclins, acting with cdk4/6, are able to phosphorylate pRb only partially, a process that is likely to be completed by cyclin E-cdk2 complexes. Furthermore, cyclin E-cdk2 is unable to phosphorylate pRb in the absence of prior phosphorylation by cyclin D-cdk4/6 complexes."
bel
"\"progression through G1 phase of cell cycle requires phosphorylation of the retinoblastoma gene product (pRb) by the cyclin D-dependent serine-threonine kinases CDK4 and CDK6"
CDK4 phosphorylates RB1 on T356. 3 / 3
1 2 |
biopax:phosphositeplus
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
CDK4 phosphorylates RB1 on S811. 3 / 3
1 1 1 |
biopax:netpath
No evidence text available
biopax:phosphositeplus
No evidence text available
signor
"Phosphorylated by cdk6 and cdk4, and subsequently by cdk2 at ser-567 in g1, thereby releasing e2f1 which is then able to activate cell growth. Here we show that although these cdks phosphorylate multiple residues in prb, they do so with different residue selectivities in vitro;thr821 and thr826 are preferentially phosphorylated by cdk6 and cdk4, respectively."
CDK4 phosphorylates RB1 on S608. 3 / 3
1 | 1 1
sparser
"This study shows that Ser-608 in pRb was phosphorylated in vitro by Cdk4 and Cdk6."
biopax:phosphositeplus
No evidence text available
reach
"We then directly examined Rb phosphorylation in MECs of different genotypes by using an antibody specifically recognizing Rb proteins phosphorylated at Ser608 by CDK4 and CDK6 [XREF_BIBR, XREF_BIBR], the functional targets of p16."
CDK4 phosphorylates RB1 on serine. 2 / 2
| 2
reach
"CDK4 and CDK6 specific phosphorylation of Rb on serine 807-811 (pSRb) confirmed that the CDK4 and cyclin D1 holoenzyme was active in MCL cells, along with CDK6 and cyclin D1 in MCL7 and CDK4 and cyclin D2 in MCL6."
reach
"PD 0332991 completely inhibited CDK4 and CDK6 within 24 hours, as indicated by the loss of CDK4 and CDK6 specific phosphorylation of Rb on serine 807-811 (pRb), arrested the cell cycle in G1, and marked reduction of DNA replication as measured by BrdU pulse labeling (XREF_FIG)."
CDK4 phosphorylates RB1 on S807. 2 / 2
1 1 |
biopax:phosphositeplus
No evidence text available
biopax:netpath
No evidence text available
CDK4 phosphorylates RB1 on S612. 1 / 1
1 |
biopax:phosphositeplus
No evidence text available
CDK4 phosphorylates RB1 on T373. 1 / 1
1 |
biopax:phosphositeplus
No evidence text available
Kinase-active CDK4 leads to the phosphorylation of RB1 on S788. 1 / 1
1 |
bel
"The pRb phosphorylated in vitro by Cdk4 was inactive as a growth suppressor upon microinjection, but pRb phosphorylated by Cdk2 or Cdk3 retained cell cycle arrest activity. While these kinases efficiently phosphorylate a common set of residues, including S373 and S788, cyclin D1/Cdk4 was a much more efficient kinase for S795 than the other kinases examined."
Kinase-active CDK4 leads to the phosphorylation of RB1 on S795. 1 / 1
1 |
bel
"The pRb phosphorylated in vitro by Cdk4 was inactive as a growth suppressor upon microinjection, but pRb phosphorylated by Cdk2 or Cdk3 retained cell cycle arrest activity. While these kinases efficiently phosphorylate a common set of residues, including S373 and S788, cyclin D1/Cdk4 was a much more efficient kinase for S795 than the other kinases examined."
CDK4 leads to the phosphorylation of RB1 on T821. 1 / 1
| 1
reach
"We found that Costello syndrome fibroblasts display elevated level of Rb phosphorylation on serine 780 (Ser (P)-780-Rb) and that pharmacological inhibition of Ras with radicicol, Mek and Erk with PD98059, or cyclin dependent kinase 4 with PD0332991 not only leads to down-regulation of Ser (P)-780-Rb levels but also enhances Rb phosphorylation on threonine 821 (Thr (P)-821-Rb), which coincides with the recovery of elastin production."
Mutated CDK4 leads to the phosphorylation of RB1. 1 / 1
| 1
reach
"Thus, the mutant CDK4 Cyclin D complex induces phosphorylation of RB protein in the host cell, based on the high homology among multiple species."
CDK4 phosphorylates RB1 on T5. 1 / 1
1 |
hprd
No evidence text available
CDK4 phosphorylates RB1 on S249. 1 / 1
1 |
signor
"Phosphorylated by cdk6 and cdk4, and subsequently by cdk2 at ser-567 in g1, thereby releasing e2f1 which is then able to activate cell growth. Here we show that although these cdks phosphorylate multiple residues in prb, they do so with different residue selectivities in vitro;thr821 and thr826 are preferentially phosphorylated by cdk6 and cdk4, respectively."
CDK4 phosphorylates RB1 on T252. 1 / 1
1 |
biopax:phosphositeplus
No evidence text available