IndraLab

Statements

USP14 affects DVL
| 11
USP14 deubiquitinates DVL. 10 / 11
| 11
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"Deubiquitination of Dishevelled by Usp14 is required for Wnt signaling."
23958854
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"These data suggest that deubiquitination of Dvl by Usp14 is necessary for the interaction between Fzd and Dvl."
23958854
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"A recent report has shown that deubiquitination of disheveled (Dvl) by USP14 is required for Wnt signaling."
29326710
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"Therefore, deubiquitination of Dvl by Usp14 may occur via their transient interaction during Wnt signal transduction."
23958854
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"To test direct deubiquitination of Dvl by Usp14, we performed a ubiquitin chain trimming assay using immunopurified Dvl-ubiquitin conjugates and recombinant Usp14 in a proteasome-free condition (XREF_FIG and XREF_SUPPLEMENTARY)."
23958854
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"Contrary to its established mechanistic role, Usp14 mediates deubiquitination of Dvl without a requirement for its UBL domain."
23958854
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"Inhibition of USP14 increases Dvl polyubiquitination and significantly impairs downstream Wnt signaling."
32486158
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"As a lack of Dvl deubiquitination by Usp14 appears to attenuate Wnt signaling, we proposed that an elevated forward rate of ubiquitination, which will be counteracted by Usp14 activity, might be induced by Wnt3a CM treatment."
23958854
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"Effects of USP14 inhibitor IU1 confirmed that inhibition of USP14 by IU1 increases the K4 linked polyubiquitination of Dvl [XREF_BIBR]."
32486158
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"As inhibition of Usp14 activity increased Dvl polyubiquitination, we examined whether knockdown of Usp14 has any effect on Wnt signaling."
23958854
USP14 affects CXCR4
1 | 8
USP14 deubiquitinates CXCR4. 7 / 7
1 | 6
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"These data suggest that the USP14 gene product indeed serves as a catalyst to deubiquitinate the CXCR4, because when its expression is reduced there is a reciprocal increase in CXCR4 ubiquitination."
19106094
ubibrowser
"Co-localization of CXCR4 and USP14 also is time-dependent following CXCL12 stimulation."
19106094
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"Deubiquitination of CXCR4 by USP14 would thus be expected to reduce the rate of ligand accelerated receptor degradation and result in an increased steady state level of receptors."
19106094
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"Deubiquitination of CXCR4 by USP14 Is Critical for Both CXCL12 induced CXCR4 Degradation and Chemotaxis but Not ERK Activation *."
19106094
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"The physical interaction of CXCR4 and USP14 is paralleled by USP14 catalyzed deubiquitination of the receptor; knockdown of endogenous USP14 by RNA interference (RNAi) blocks CXCR4 deubiquitination, whereas overexpression of USP14 promotes CXCR4 deubiquitination."
19106094
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"In summary, our findings demonstrate that CXCL12 activation of the CXCR4 leads to a dynamic ubiquitination and deubiquitination cycle and that USP14 preferentially interacts with and deubiquitinates CXCR4."
19106094
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"Deubiquitination of CXCR4 by USP14 is critical for both CXCL12 induced CXCR4 degradation and chemotaxis but not ERK ativation."
19106094
Modified USP14 leads to the deubiquitination of CXCR4. 2 / 2
| 2
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"Overexpression of USP14 promotes CXCR4 deubiquitination and allows it to escape degradation [XREF_BIBR, XREF_BIBR]."
28448471
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"The physical interaction of CXCR4 and USP14 is paralleled by USP14 catalyzed deubiquitination of the receptor; knockdown of endogenous USP14 by RNA interference (RNAi) blocks CXCR4 deubiquitination, whereas overexpression of USP14 promotes CXCR4 deubiquitination."
19106094
USP14 affects AR
1 | 6
USP14 deubiquitinates AR. 7 / 7
1 | 6
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"USP14 inhibits AR ubiquitination and subsequent degradation in both prostate and breast cancer cells [77, 78]."
30217566
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"Treatment of control MDA-MB-453 (either scramble shRNA or parental) cells with CHX for up to 12h caused decreased levels of AR, suggesting a contribution of AR protein synthesis to endogenous AR protein levels; however, co-treatment of CHX and USP14 shRNA or IU1 resulted more rapid decrease in levels of endogenous AR protein, strongly suggest that deubiquitination of AR protein by USP14 is essential for its protein stability."
29353883
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"As a deubiquitinating enzyme, USP14 has previously been reported to enhance the deubiquitination of AR and resist the binding of MDM2, but the specific mechanism is still unclear and needs further exploration."
34759344
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"In addition, reduction of UCHL5 by its siRNA did not affect the expression of AR, suggesting that USP14 but not UCHL5 recruited on the19S proteasome plays a selective role in the deubiquitination of AR."
29353883
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"We found that IU1 and USP14 knockdown dramatically increased levels of ubiquitinated and K48 ubiquitinated AR, suggesting that USP14 is an AR DUB, capable of deubiquitinating and thereby stabilizing AR protein."
29353883
ubibrowser
"Additionally, both genetic and pharmacological inhibition of USP14 significantly suppressed cell proliferation in AR-responsive breast cancer cells by blocking G0/G1 to S phase transition and inducing apoptosis."
29353883
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"Moreover, AR overexpression inhibited USP14 inhibition induced events, suggesting that AR deubiquitination by USP14 is critical for breast cancer growth and USP14 inhibition is a possible strategy to treat AR positive breast cancer."
29353883
USP14 affects Proteasome
| 5
USP14 deubiquitinates Proteasome. 2 / 2
| 2
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"Given that proteasome deubiquitination mediated by USP14 has fundamental roles in regulating proteasomal degradation of ubiquitinylated substrates XREF_BIBR XREF_BIBR, it is rational to speculate that perturbation of ubiquitin chain trimming functions of PfUSP14 may impact intraerythrocytic parasite development and cause difficulties for parasite egress from the host cell."
26639022
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"USP14 deubiquitinates proteasome bound substrates that are ubiquitinated at multiple sites."
27074503
USP14 deubiquitinates Proteasome on S26. 2 / 2
| 2
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"Thus Uch37 and Usp14 may both deubiquitinate the 26S proteasome."
24743594
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"Thus the DUB activities of Uch37 and/or Usp14 appear to antagonize ubiquitination of the 26S proteasome."
24743594
USP14 leads to the deubiquitination of Proteasome on K48. 1 / 1
| 1
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"We found that inhibition of USP14 accelerated the K48 ubiquitination and proteasome mediated degradation of AR protein."
29353883
USP14 affects NLRC5
1 | 4
USP14 deubiquitinates NLRC5. 5 / 5
1 | 4
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"The ubiquitination of NLRC5 could be reversely regulated by USP14."
26620909
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"Next, we determined whether USP14 inhibited NLRC5 ubiquitination through its DUB activity."
26620909
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"In an effort to elucidate the mechanisms underlying this regulation, the Cui group showed that TLR4 stimulation activates the TRAF2/6 complex, which ubiquitinates NLRC5 on Lys1178 residue, presumably leading to its degradation and release of IKKalpha and IKKbeta to complex with IKKgamma [XREF_BIBR, XREF_BIBR] (XREF_FIG) This study also showed that the ubiquitin specific protease 14 (USP14) deubiquitinates NLRC5 to sustain the NLRC5 mediated inhibition of NF-kappaB activation."
33671123
ubibrowser
"The researchers propose that, after the ubiquitination of NLRC5 at lysine 1178 is catalyzed by TRAF2/6, USP14 specifically removes the polyubiquitin chains from NLRC5 to enhance NLRC5-mediated inhibition of IKK–NF-κB signaling, thus forming a coherent feedforward loop to regulate IKK–NF-κB activation"
26620908
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"These results suggest that USP14 inhibits NLRC5 ubiquitination through its DUB activity."
26620909
USP14 affects TAB2
1 | 3
USP14 leads to the deubiquitination of TAB2. 4 / 4
1 | 3
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"These results suggest that USP14 induces the deubiquitination of TAB 2."
29326710
ubibrowser
"Taken together, our data demonstrate that USP14 can negatively regulate autophagy induction by inhibiting Beclin 1 ubiquitination, interrupting association between TRAF6 and Beclin 1, and affecting TLR4-induced activation of NF-魏B through deubiquitination of TAB 2 protein"
29326710
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"Since USP14 induced the deubiquitination of TAB 2, we then examined the functional regulation of TLR4 mediated signaling in Ctrl and USP14 KD THP-1 cells."
29326710
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"USP14 Induces Deubiquitination of TAB 2 and Inhibits TLR4 Mediated Signaling."
29326710
USP14 affects VIM
1 | 2
USP14 deubiquitinates VIM. 3 / 3
1 | 2
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"According to the above results, USP14 de-ubiquitinates vimentin and increases its expression levels, which may influence the aggressiveness of GC cells."
27448976
ubibrowser
"Furthermore, USP14 can also deubiquitinate and stabilize vimentin, a vital protein which involves in epithelial-to-mesenchymal transition(EMT) and significantly promotes cell growth, migration and invasion in human gastric cancer"
30319415
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"Furthermore, USP14 can also deubiquitinate and stabilize vimentin, a vital protein which involves in epithelial-to-mesenchymal transition(EMT) and significantly promotes cell growth, migration and invasion in human gastric cancer (Zhu et al., 2017)."
30319415
USP14 affects CREBBP
| 3
USP14 deubiquitinates CREBBP. 2 / 2
| 2
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"To investigate whether USP14 deubiquitylated CBP, we transfected MLE12 cells to overexpress USP14 or treated them with IU1, and then we examined the ubiquitylation of CBP."
28611184
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"Furthermore, we revealed that USP14 deubiquitylated CBP and promoted its stability."
28611184
Modified USP14 leads to the deubiquitination of CREBBP. 1 / 1
| 1
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"We found that overexpression of USP14 reduced CBP ubiquitylation, whereas IU1 had the opposite effect (XREF_FIG)."
28611184
USP14 affects BECN1
1 | 2
USP14 deubiquitinates BECN1. 3 / 3
1 | 2
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"The deubiquitination of BECLIN 1 by USP14 inhibits the PtdIns3P production of VPS34."
29186924
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"Based on these previous findings, we hypothesized that the suppression of Beclin 1 ubiquitination by USP14 might be critically associated with TRAF6 mediated ubiquitination in both autophagy and TLR4 mediated signaling."
29326710
ubibrowser
"USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1"
27542828
USP14 affects PI3K
| 2
USP14 deubiquitinates PI3K. 2 / 2
| 2
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"Mechanistically, USP14 deubiquitinates and activates PI3K/AKT in HCC cells."
34900376
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"In addition, USP14 was observed to upregulate the Wnt/β-catenin signaling mediated by cleaving K63-linked polyubiquitin chains (Table 2, Figure 6) (Jung et al., 2013) (Huang et al., 2015), and promote the proliferation and metastasis of HCC cells by deubiquitinating and activating PI3K (Table 2) (Wright et al., 2019; Zhang Y. et al., 2020)."
35069211
USP14 affects IL6
| 2
USP14 deubiquitinates IL6. 2 / 2
| 2
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"IL-6 protein is deubiquitinated by USP14 and upregulated in LPS-treated GMECs, further promoting ferroptosis and inflammation through the NRF2 signaling pathway."
35549778
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"Further, ubiquitin experiment and co-immunoprecipitation assay showed that USP14 upregulated IL-6 protein expression by reducing the ubiquitination of IL-6, and overexpression of IL-6 reversed the inhibitory effect of USP14 shRNA on LPS-treated GMECs ferroptosis."
35549778
USP14 affects FASN
| 2
USP14 deubiquitinates FASN. 2 / 2
| 2
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"As shown in Figure 4b,c, USP14 slightly reduced FASN ubiquitination."
| PMC
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"Since USP14 reduced FASN protein levels in cancer cells, it was necessary to confirm the deubiquitination of FASN by USP14."
| PMC
USP14 affects DDX58
1 | 1
USP14 deubiquitinates DDX58. 2 / 2
1 | 1
ubibrowser
"n this study, we demonstrated USP14, a deubiquitinating enzyme, as a negative regulator in antiviral responses by directly deubiquitinating K63-linked RIG-I."
30466171
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"Furthermore, USP14 is a negative regulator in antiviral responses because it directly deubiquitinates K63-linked ubiquitin on retinoic acid-inducible gene I (RIG-I), which is a critical RNA virus sensor that initiates the antiviral immune response (31)."
33968009
USP14 affects CGAS
| 2
USP14 deubiquitinates CGAS. 2 / 2
| 2
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"TRIM14 has been reported to interact with cGAS via its PRYSPRY domain and upon DNA virus infection recruit the proteasome-associated deubiquitinase (DUB) USP14 to deubiquitinate cGAS, preventing recruitment of p62 and autophagy-dependent degradation of cGAS (Jia et al., 2017) ."
29551141
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"TRIM14 has been reported to interact with cGAS via its PRYSPRY domain and upon DNA virus infection recruit the proteasome associated deubiquitinase (DUB) USP14 to deubiquitinate cGAS, preventing recruitment of p62 and autophagy dependent degradation of cGAS."
29551141
USP14 affects CCNB1
| 2
USP14 deubiquitinates CCNB1. 2 / 2
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"Inhibition or knockdown of USP14 significantly augmented the ubiquitination of cyclin B1 and arrested the cell cycle at the G2/M phase, thereby inhibiting the proliferation and migration of breast cancer cells, and offering the theoretical basis for the development of USP14-targeted anticancer drugs (Table 2) (Liu et al., 2019)."
35069211
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"Liu et al. found that USP14 modulated the cell cycle progression of breast cancer cells by deubiquitinating cyclin B1, a critical regulator of the mitotic phase (M phase) (Yu et al., 2002; Porter and Donoghue, 2003; Ding et al., 2014; Liu et al., 2019)."
35069211
USP14 deubiquitinates lipopolysaccharide. 1 / 1
| 1
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"IL-6 protein is deubiquitinated by USP14 and upregulated in LPS-treated GMECs, further promoting ferroptosis and inflammation through the NRF2 signaling pathway."
35549778
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"Consequently, USP14 deficiency may cause ataxia in the ax J mice in part by perturbing the turnover and/or cell surface distribution of GABA A R. Still, since it is not clear that USP14 directly deubiquitinates GABA A R, it is also worth considering that this DUB may regulate the receptor indirectly."
25191222
USP14 affects ZUP1
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USP14 deubiquitinates ZUP1. 1 / 1
| 1
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"Proteasomes function under tonic inhibitory conditions, possibly via the ubiquitin chain-trimming function of USP14, a proteasome-associated deubiquitinating enzyme (DUB)."
26041011
USP14 affects Wnt
| 1
USP14 leads to the deubiquitination of Wnt. 1 / 1
| 1
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"Genetic and chemical suppression of USP14 activity caused an increase in Dishevelled (Dvl) polyubiquitination and significantly impaired downstream Wnt signaling, suggesting an oncogenic role for USP14 through Wnt and beta-catenin signaling enhancement [XREF_BIBR]."
26097878
USP14 affects WT NCB1
| 1
USP14 deubiquitinates WT NCB1. 1 / 1
| 1
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"When we quenched substrate degradation by replacing ATP with ADP (XREF_FIG), USP14 dependent deubiquitination of WT NCB1 conjugates was preserved (XREF_FIG)."
27074503
USP14 affects TRIM14
| 1
USP14 deubiquitinates TRIM14. 1 / 1
| 1
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"In the absence of USP14, TRIM14 failed to inhibit K63‐linked ubiquitination of p100 and stabilize p100/p52 (Figure S7D, Supporting Information), while USP14 alone could not deubiquitinate p100 and stabilize p100/p52 without TRIM14 (Figure 5I)."
31921549
USP14 affects NFKB2
| 1
USP14 deubiquitinates NFKB2. 1 / 1
| 1
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"In the absence of USP14, TRIM14 failed to inhibit K63‐linked ubiquitination of p100 and stabilize p100/p52 (Figure S7D, Supporting Information), while USP14 alone could not deubiquitinate p100 and stabilize p100/p52 without TRIM14 (Figure 5I)."
31921549
USP14 affects MAP3K7
| 1
USP14 deubiquitinates MAP3K7. 1 / 1
| 1
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"Min et al. 20 reported that USP14 deubiquitinates TAK1‐binding protein 2 protein to active NF‐κB signaling, leading to enhance the production of proinflammatory cytokines."
34089575
USP14 affects IKBKB
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USP14 deubiquitinates IKBKB. 1 / 1
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ubibrowser
"This study suggests that USP14 removes the ubiquitin chain of I-κB, therefore inducing I-κB degradation and increasing cytokine release in lung epithelial cells."
23615914
USP14 affects HAND1
| 1
USP14 deubiquitinates HAND1. 1 / 1
| 1
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"RGS2 enhanced the protein degradation of the transcription factor heart and neural crest derivatives expressed 1 (HAND1) by suppressing ubiquitin-specific protease 14 (USP14)-mediated deubiquitination of HAND1, resulting in the restoration of HAND1-induced trans-inactivation of the aromatase gene and subsequent increases in E2 levels."
36653442
USP14 affects GABBR1
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USP14 deubiquitinates GABBR1. 1 / 1
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ubibrowser
"Post-endocytotic Deubiquitination and Degradation of the Metabotropic γ-Aminobutyric Acid Receptor by the Ubiquitin-specific Protease 14"
26817839
USP14 affects FAM126A
| 1
USP14 deubiquitinates FAM126A. 1 / 1
| 1
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"Mechanistically, USP14 deubiquitinates and activates PI3K/AKT in HCC cells."
34900376
USP14 affects ELOVL6
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USP14 deubiquitinates ELOVL6. 1 / 1
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ubibrowser
"Among the potential substrates identified, we show that fatty acid synthase (FASN), a key enzyme involved in hepatic lipogenesis, is a bona fide substrate of USP14."
30425250
USP14 affects DVL2
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USP14 deubiquitinates DVL2. 1 / 1
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ubibrowser
"Usp14 deubiquitinates K63-linked polyubiquitin chains of Dvl"
23958854
USP14 affects CyclinB1
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USP14 deubiquitinates CyclinB1. 1 / 1
| 1
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"CyclinB1 deubiquitination by USP14 regulates cell cycle progression in breast cancer."
31474315
USP14 affects CTNNB1
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USP14 deubiquitinates CTNNB1. 1 / 1
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ubibrowser
"Additionally, DUBs USP14, USP15, USP47, and Fam/USP9X have been reported to prevent β-catenin turnover by inhibiting its Ub-proteasomal degradation"
29080116
USP14 affects CDK1
| 1
USP14 deubiquitinates CDK1. 1 / 1
| 1
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"USP14 regulates cell cycle progression through deubiquitinating CDK1 in breast cancer."
36604147
USP14 affects BUR6
| 1
USP14 deubiquitinates BUR6. 1 / 1
| 1
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"USP14 deubiquitinated this form of NCB1 at a rate comparable to that of wild-type conjugates, indicating that USP14 does not act obligatorily on ubiquitin-ubiquitin linkages (XREF_FIG)."
27074503
USP14 affects AURKB
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USP14 deubiquitinates AURKB. 1 / 1
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ubibrowser
"The Deubiquitinating Enzyme USP14 Regulates Leukemic Chemotherapy Drugs-Induced Cell Apoptosis by Suppressing Ubiquitination of Aurora Kinase B"
28662510
USP14 affects AKT
| 1
USP14 deubiquitinates AKT. 1 / 1
| 1
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"Mechanistically, USP14 deubiquitinates and activates PI3K/AKT in HCC cells."
34900376