IndraLab

Statements

USP45 affects ERCC1
1 2 7 1 | 22 21
USP45 binds ERCC1.
7 | 5 20
USP45 binds ERCC1. 10 / 33
7 | 5 16
reach
"This implies that the rescue of the sensitivity phenotype depends on the interaction between ERCC1 and USP45 as well as its catalytic activity."
25538220
sparser
"USP45 associates with ERCC1 and depletion results in increased levels of ubiquitylated ERCC1, leading to hypersensitivity to UV irradiation [ xref ]."
38572758
sparser
"Another study [ xref ] demonstrated a direct interaction between the deubiquitylase USP45 and ERCC1, which may control its ubiquitylation."
30563071
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
sparser
"USP45 associates with ERCC1, a subunit of the DNA repair endonuclease XPF–ERCC1, via a short acidic motif outside of the USP45 catalytic domain."
25538220
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
sparser
"USP45 interacts with ERCC1."
25538220
USP45 binds ERCC4 and ERCC1. 4 / 4
| 4
sparser
"USP45 associates with the ERCC1–XPF endonuclease."
25538220
sparser
"Upon UV exposure, USP45 interacts with ERCC1–XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that cannot bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
sparser
"These data suggested that USP45 interacts directly with XPF–ERCC1 and not with SLX4."
25538220
sparser
"USP45 interacts specifically with ERCC1–XPF via its N-terminal 61 residues."
25538220
USP45 deubiquitinates ERCC1.
1 1 1 | 12
USP45 deubiquitinates ERCC1. 10 / 11
1 | 10
reach
"It was shown that USP45 interacts with and deubiquitinates the excision repair cross complementation group 1 (ERCC1) subunit of the XPF-ERCC1 DNA repair nuclease which has role in the TC-NER of UV induced DNA damage [XREF_BIBR, XREF_BIBR]."
30831436
reach
"ERCC1 can be deubiquitinated by USP45, which promotes XPF-ERCC1 recruitment to sites of DNA damage [XREF_BIBR]."
27827925
reach
"Upon UV exposure, USP45 interacts with ERCC1-XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that can not bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
reach
"The major role of ERCC1 deubiquitination by USP45 has been speculated to enable ERCC1-XPF to gain access to DNA damage sites."
27827925
reach
"In this light, we observed that 5 other DUBs tested (AMSH, Cezanne, OTUB1, UCHL1 and USP27X) failed to deubiquitylate ERCC1 under conditions that wild-type USP45 deubiquitylated ERCC1 (Supplementary Fig S5C)."
25538220
reach
"This, coupled with the report that inactivating the USP45 protein that deubiquitinates ERCC1 in human myelogenous leukaemia and osteosarcoma cells leads to reduced levels of NER and increased sensitivity to UV irradiation [XREF_BIBR], suggests that our therapeutic strategy is likely applicable to a wide range of human cancers."
28903417
reach
"These data therefore indicate that likely the ubiquitylation of ERCC1, which is reversed by USP45, controls an aspect of ERCC1 function other than regulating its stability."
25538220
ubibrowser
"<span class="match term0">USP45</span> targeting <span class="match term1">ERCC1</span>"
27316462
reach
"Thus, the major role of ERCC1 deubiquitylation by USP45 may be to enable ERCC1-XPF to gain access to DNA damage sites."
25538220
reach
"As expected, the catalytically inactive USP45 [Cys199Ala] mutant also failed to deubiquitylate ERCC1 (Fig XREF_FIG C, XREF_SUPPLEMENTARY)."
25538220
Mutated USP45 deubiquitinates ERCC1. 2 / 2
| 2
reach
"Importantly, the USP45 mutant [Asp25Ala, Glu26Ala] that displays the same intrinsic catalytic activity as wild-type USP45 when assayed using a generic ubiquitin-rhodamine substrate (XREF_SUPPLEMENTARY), but that can not bind ERCC1, failed to deubiquitylate ERCC1 in parallel experiments (Fig XREF_FIG C, XREF_SUPPLEMENTARY)."
25538220
reach
"Importantly, we find that the USP45 mutant [Asp25Ala, Glu26Ala] that is catalytically active but that can not bind to ERCC1 (Supplementary Fig S5A) failed to deubiquitylate ERCC1 in vitro, indicating that specific interaction between ERCC1 and USP45 is essential to trigger deubiquitylation."
25538220
USP45 deubiquitinates ERCC1 bound to ERCC4. 1 / 1
1 |
signor
"USP45 associates with the ERCC1–XPF endonuclease. USP45 interacts specifically with ERCC1–XPF via its N-terminal 61 residues. USP45 deubiquitylates ERCC1. USP45 promotes survival of cells exposed to agents that induce DNA damage responses controlled by ERCC1–XPF endonuclease"
25538220
USP45 deubiquitinates ERCC1 on lysine. 1 / 1
1 |
biopax:reactome
No evidence text available
USP45 activates ERCC1.
1 | 3
USP45 activates ERCC1. 3 / 4
| 3
reach
"USP45 targeting ERCC1."
27316462
reach
"Moreover, expression of wild-type USP45, but not catalytically deficient USP45 [Cys199Ala] mutant in these cells, restored the number of spontaneous ERCC1 foci almost to the level seen in wild-type U2OS cells (Fig XREF_FIG A and B)."
25538220
reach
"Expression of wild-type USP45, but not the catalytically deficient USP45 [Cys199Ala] mutant in USP45 knockout U2OS cells, restored the number of DNA damage induced ERCC1 foci to levels observed in wild-type cells (Fig XREF_FIG A and B)."
25538220
USP45 activates ERCC1 bound to ERCC4. 1 / 1
1 |
signor
"USP45 associates with the ERCC1–XPF endonuclease. USP45 interacts specifically with ERCC1–XPF via its N-terminal 61 residues. USP45 deubiquitylates ERCC1. USP45 promotes survival of cells exposed to agents that induce DNA damage responses controlled by ERCC1–XPF endonuclease"
25538220
USP45 inhibits ERCC1.
| 2 1
USP45 inhibits ERCC1. 3 / 4
| 2 1
reach
"Restoring expression of wild-type USP45 in knockout U2OS cells markedly lowered the high molecular weight species of ERCC1 (Fig XREF_FIG B)."
25538220
sparser
"It was shown that USP45 could inhibit the ubiquitin-proteasome degradation pathway of ERCC1 protein, a DNA repair-related nucleic acid endonuclease, thereby enhancing the ability of tumor cells to repair DNA damage. xref KEGG pathway analysis showed that USP45 may be associated with the MAPK signaling pathway, AMPK signaling pathway, Hippo signaling pathway, cell cycle, p53 signaling pathway, and TGF-β signaling pathway ( xref N and xref )."
38868575
reach
"It was shown that USP45 could inhibit the ubiquitin-proteasome degradation pathway of ERCC1 protein, a DNA repair-related nucleic acid endonuclease, thereby enhancing the ability of tumor cells to repair DNA damage.4 KEGG pathway analysis showed that USP45 may be associated with the MAPK signaling pathway, AMPK signaling pathway, Hippo signaling pathway, cell cycle, p53 signaling pathway, and TGF-β signaling pathway (Fig. 1N and Table S5)."
38868575
Peptide affects USP45
| 39
Peptide activates USP45. 10 / 39
| 39
reach
"On the one hand, it may be because the fusion of the initial signal peptide with Usp45 signal peptide would affect the folding of the signal peptide, which in turn affects its function."
36608408
reach
"Whole cell ELISA assay showed that pSD1nuc which had the combination of P promoter with USP45 signal peptide gave very low signals in the ELISA assay, although fluorescent signals were still detectable in immunofluorescence assay (Fig. 7)."
31775775
reach
"A cassette consisting of USP45 fused with LEISSTCDA propeptide to increase secretion efficiency, pNZ8048 multiple cloning sites (MCS) modified to include more RE sites, pNZ8048 terminator (T), and TP901–1 bacteriophage attachment site (attP) was synthesized by Integrated DNA Technologies (Singapore) and denoted as attP cassette.The vectors constructions strategy was divided into four phases; Phase I) Construction of PZatt cassette composed of the P promoter, USP45 signal peptide, LEISSTCDA propeptide and attP sequence; Phase II) Construction of the secretion integrative vectors (pS1–4); (a) Plasmid circularisation by incorporation of erythromycin resistant gene and E. coli replication origin into the PZatt cassette, denoted as pS1; (b) Replacement of P promoter with P promoter to produce pS3 plasmid; (c) Replacement of USP45-LEISSTCDA fusion with SPK1 signal peptide into each pS1 and pS3 plasmids to produce pS2 and pS4 plasmids (Additional file 3: Figure S1); Phase III) Construction of the surface display integrative vectors (pSD1–4) by incorporation of PrtP anchor domain into each secretion integrative vector; Phase IV) Cloning of reporter gene, nuclease (Additional file 4: Figure S2)."
31775775
reach
"The present investigation included cloning the BAB1-0278 gene fragment, which contains 237 base pairs coupled with the Usp45 signal peptide, into the pNZ8148 vector using K pnl and X bal restriction e[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37866552
reach
"Substantially higher levels of the fusion proteins (four- to six-fold) were secreted by the clones possessing Lactobacillus brevis SlpA signal peptide than by those possessing L. lactis Usp45 signal peptide."
21901278
reach
"On the other hand, the pS2 plasmid was constructed by replacing USP45 signal peptide with SPK1 using EcoRV and KpnI RE."
31775775
reach
"To obtain BaMTGase into the medium, the DNA encoding Usp45 signal peptide (SPusp45) was fused to the B. amyloliquefaciens mtg gene to direct the secretion."
36286175
reach
"Secretion using Usp45 signal peptide generally gave satisfactory efficiency."
21968145
reach
"For example, using Usp45 signal peptide, a Bacillus nattokinase was almost completely secreted to the medium ( Liang et al., 2006 )."
21968145
reach
"Compared to the control which utilizes Usp45 signal peptide, the new signal peptide allowed much higher secretion of mIL-12, 185 pg/ml versus 40 pg/ml ( Fernandez et al., 2009 )."
21968145
ERCC1 affects USP45
7 | 5 20
USP45 binds ERCC1. 10 / 33
7 | 5 16
reach
"This implies that the rescue of the sensitivity phenotype depends on the interaction between ERCC1 and USP45 as well as its catalytic activity."
25538220
sparser
"USP45 associates with ERCC1 and depletion results in increased levels of ubiquitylated ERCC1, leading to hypersensitivity to UV irradiation [ xref ]."
38572758
sparser
"Another study [ xref ] demonstrated a direct interaction between the deubiquitylase USP45 and ERCC1, which may control its ubiquitylation."
30563071
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
sparser
"USP45 associates with ERCC1, a subunit of the DNA repair endonuclease XPF–ERCC1, via a short acidic motif outside of the USP45 catalytic domain."
25538220
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
sparser
"USP45 interacts with ERCC1."
25538220
USP45 binds ERCC4 and ERCC1. 4 / 4
| 4
sparser
"USP45 associates with the ERCC1–XPF endonuclease."
25538220
sparser
"Upon UV exposure, USP45 interacts with ERCC1–XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that cannot bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
sparser
"These data suggested that USP45 interacts directly with XPF–ERCC1 and not with SLX4."
25538220
sparser
"USP45 interacts specifically with ERCC1–XPF via its N-terminal 61 residues."
25538220
USP45 affects MYC
3 | 18 5
USP45 binds MYC.
3 | 4 5
USP45 binds MYC. 10 / 11
3 | 3 5
biogrid
No evidence text available
36765885
biogrid
No evidence text available
36765885
sparser
"Therefore, we explored the regulatory mechanism of USP45-MYC in cervical cancer."
36765885
sparser
"Co-IP assays demonstrated that USP45 interacted directly with MYC, and the interaction was independent to the USP45 catalytic activity ( xref A–C)."
36765885
sparser
"The purified USP45 was incubated with purified MYC from HEK293T cells, followed by the pulling down experiments, which demonstrated the direct interaction between USP45 and MYC ( xref F)."
36765885
sparser
"Herein we report our discoveries in this research area, and we have found that deubiquitinase USP45 can directly bind with MYC, resulting in its deubiquitination and stabilization."
36765885
reach
"Herein we report our discoveries in this research area, and we have found that deubiquitinase USP45 can directly bind with MYC, resulting in its deubiquitination and stabilization."
36765885
sparser
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
reach
"USP45 Interacts with and Deubiquitinates MYC."
36765885
reach
"The purified USP45 was incubated with purified MYC from HEK293T cells, followed by the pulling down experiments, which demonstrated the direct interaction between USP45 and MYC (Figure 5F)."
36765885
USP45 binds MYC and K48. 1 / 1
| 1
reach
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
USP45 activates MYC.
| 8
USP45 activates MYC. 8 / 8
| 8
reach
"Further, USP45 overexpressing can upregulate MYC, and this overexpressing can significantly enhance cancer development, cancer cell stemness and drug resistance."
36765885
reach
"Furthermore, we have found that USP45 inhibition with a natural small molecule promoted MYC degradation and, in turn, reduced MYC-mediated stemness and drug resistance in cervical cancer."
36765885
reach
"We found that USP45 overexpressing significantly upregulated MYC (Figure 1A), and USP45 inhibition with the serval siRNAs significantly decreased MYC (Figure 1B)."
36765885
reach
"The MYC decrease by USP45 inhibition with siRNA was reversed by overexpression of USP45 (Figure 1C) or by the addition of the proteasome inhibitor MG132 (Figure 1D)."
36765885
reach
"MYC regulation by USP45 was dependent on its DUB activity (Figure 1E), and the USP45 resulted in MYC elevation in a dose-dependent manner (Figure 1F), while its C199A mutant did not have any impact on the MYC level."
36765885
reach
"Further, we have discovered that increased USP45 expression significantly promoted cervical cancer cell proliferation, stemness and drug resistance, while USP45-enhanced MYC stability facilitated only stemness and drug resistance."
36765885
reach
"Furthermore, through RT-qPCR and Western blot, we confirm that USP45 overexpression up-regulated the mRNA and protein levels of MYC and ATAD2, while USP45 knockdown down-regulated MYC and ATAD2."
38868575
reach
"USP45 has been demonstrated to modulate the stability of Spindly and MYC proteins through deubiquitination (Conte et al., 2018; Tu et al., 2023)."
40067150
USP45 inhibits MYC.
| 2
USP45 inhibits MYC. 2 / 2
| 2
reach
"In conclusion, we have demonstrated that USP45 may play a critical role in enhancing cell survival after cancer treatments, and USP45 inhibition causing MYC destabilization may provide a new strategy for clinical CSC treatments."
36765885
reach
"We found that USP45 overexpressing significantly upregulated MYC (Figure 1A), and USP45 inhibition with the serval siRNAs significantly decreased MYC (Figure 1B)."
36765885
USP45 increases the amount of MYC.
| 2
USP45 increases the amount of MYC. 2 / 2
| 2
reach
"Furthermore, through RT-qPCR and Western blot, we confirm that USP45 overexpression up-regulated the mRNA and protein levels of MYC and ATAD2, while USP45 knockdown down-regulated MYC and ATAD2."
38868575
reach
"USP45 Upregulates MYC Protein Level and Stability."
36765885
USP45 deubiquitinates MYC.
| 2
USP45 deubiquitinates MYC. 2 / 2
| 2
reach
"Ubiquitination assays demonstrated that USP45 overexpression directly reduced the ubiquitination of MYC (Figure 2E)."
36765885
reach
"USP45 Interacts with and Deubiquitinates MYC."
36765885
USP45 affects SPDL1
2 4 | 3 7
USP45 binds SPDL1.
4 | 1 7
USP45 binds SPDL1. 10 / 11
4 | 1 6
reach
"Conte identified hSpindly as a new target of the ubiquitin-specific protease USP45; hSpindly and USP45 can form a complex whose interaction is specifically related to the catalytic activity of USP45 [38]."
38623460
biogrid
No evidence text available
30258100
sparser
"Conte identified hSpindly as a new target of the ubiquitin-specific protease USP45; hSpindly and USP45 can form a complex whose interaction is specifically related to the catalytic activity of USP45 [ xref ]."
38623460
sparser
"These results further underline the importance of DUB enzymatic activity in the interaction between USP45 and Spindly."
30258100
biogrid
No evidence text available
30258100
sparser
"Given that USP45 is a DUB, we decided to explore the ubiquitylation in the Spindly-USP45 complex."
30258100
biogrid
No evidence text available
30258100
sparser
"To validate the data of mass spectrometry, co-immunoprecipitation assays were performed using endogenous USP45 followed by immunoblot that validated the interaction between endogenous Spindly and endogenous USP45 (Fig.  xref )."
30258100
sparser
"This could be explained if the interaction is through the ubiquitin motif as data from the yeast two hybrid assay reveals that the interaction between USP45 and Spindly is not direct or could be through a third, unknown ubiquitylated protein in the complex."
30258100
sparser
"In order to map the region of USP45 interacting with Spindly, we performed co-immunoprecipitation studies from HEK293 cells transiently expressing different USP45 fragments GFP tagged (Fig.  xref )."
30258100
USP45 binds FlaG and SPDL1. 1 / 1
| 1
sparser
"This suggests that Spindly could be potentially be mono-ubiquitylated instead of poly-ubiquitylated: Treatment of the IP of Spindly-FLAG with recombinant USP45 WT showed a removal of the potential Spindly mono-ubiquitylation band (Fig.  xref , right panel)."
30258100
USP45 deubiquitinates SPDL1.
1 | 1
USP45 deubiquitinates SPDL1. 2 / 2
1 | 1
reach
"USP45 was found to promote cell migration by catalyzing the deubiquitination of SPDL1 protein and upregulating its protein expression level (Conte et al., 2018)."
35836933
signor
"Spindly is mono-ubiquitylated and this ubiquitin can be removed by active USP45. K48 ubiquitylated complex that interacts with Spindly is also de-ubiquitylated by USP45. In the absence of USP45 catalytic activity, interaction is abolished and cell migration is affected similarly to the phenotype described for lack of Spindly."
30258100
USP45 ubiquitinates SPDL1.
| 1
USP45 leads to the ubiquitination of SPDL1. 1 / 1
| 1
reach
"Additionally, inhibiting USP45 leads to the failure of cell migration by blocking the deubiquitination of SPDL1 protein and downregulating its protein expression level.126 Both MARK4 and USP45 are highly promising as general cancer treatment targets."
39469723
USP45 activates SPDL1.
1 |
USP45 activates SPDL1. 1 / 1
1 |
signor
"Spindly is mono-ubiquitylated and this ubiquitin can be removed by active USP45. K48 ubiquitylated complex that interacts with Spindly is also de-ubiquitylated by USP45. In the absence of USP45 catalytic activity, interaction is abolished and cell migration is affected similarly to the phenotype described for lack of Spindly."
30258100
MYC affects USP45
3 | 5 5
MYC binds USP45.
3 | 4 5
USP45 binds MYC. 10 / 11
3 | 3 5
biogrid
No evidence text available
36765885
biogrid
No evidence text available
36765885
sparser
"Therefore, we explored the regulatory mechanism of USP45-MYC in cervical cancer."
36765885
sparser
"Co-IP assays demonstrated that USP45 interacted directly with MYC, and the interaction was independent to the USP45 catalytic activity ( xref A–C)."
36765885
sparser
"The purified USP45 was incubated with purified MYC from HEK293T cells, followed by the pulling down experiments, which demonstrated the direct interaction between USP45 and MYC ( xref F)."
36765885
sparser
"Herein we report our discoveries in this research area, and we have found that deubiquitinase USP45 can directly bind with MYC, resulting in its deubiquitination and stabilization."
36765885
reach
"Herein we report our discoveries in this research area, and we have found that deubiquitinase USP45 can directly bind with MYC, resulting in its deubiquitination and stabilization."
36765885
sparser
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
reach
"USP45 Interacts with and Deubiquitinates MYC."
36765885
reach
"The purified USP45 was incubated with purified MYC from HEK293T cells, followed by the pulling down experiments, which demonstrated the direct interaction between USP45 and MYC (Figure 5F)."
36765885
USP45 binds MYC and K48. 1 / 1
| 1
reach
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
MYC inhibits USP45.
| 1
MYC inhibits USP45. 1 / 1
| 1
reach
"Interestingly, without enhancing cancer development, MYC silencing with shRNA can only suppress USP45-induced stemness and drug resistance."
36765885
SPDL1 affects USP45
4 | 1 7
USP45 binds SPDL1. 10 / 11
4 | 1 6
reach
"Conte identified hSpindly as a new target of the ubiquitin-specific protease USP45; hSpindly and USP45 can form a complex whose interaction is specifically related to the catalytic activity of USP45 [38]."
38623460
biogrid
No evidence text available
30258100
sparser
"Conte identified hSpindly as a new target of the ubiquitin-specific protease USP45; hSpindly and USP45 can form a complex whose interaction is specifically related to the catalytic activity of USP45 [ xref ]."
38623460
sparser
"These results further underline the importance of DUB enzymatic activity in the interaction between USP45 and Spindly."
30258100
biogrid
No evidence text available
30258100
sparser
"Given that USP45 is a DUB, we decided to explore the ubiquitylation in the Spindly-USP45 complex."
30258100
biogrid
No evidence text available
30258100
sparser
"To validate the data of mass spectrometry, co-immunoprecipitation assays were performed using endogenous USP45 followed by immunoblot that validated the interaction between endogenous Spindly and endogenous USP45 (Fig.  xref )."
30258100
sparser
"This could be explained if the interaction is through the ubiquitin motif as data from the yeast two hybrid assay reveals that the interaction between USP45 and Spindly is not direct or could be through a third, unknown ubiquitylated protein in the complex."
30258100
sparser
"In order to map the region of USP45 interacting with Spindly, we performed co-immunoprecipitation studies from HEK293 cells transiently expressing different USP45 fragments GFP tagged (Fig.  xref )."
30258100
USP45 binds FlaG and SPDL1. 1 / 1
| 1
sparser
"This suggests that Spindly could be potentially be mono-ubiquitylated instead of poly-ubiquitylated: Treatment of the IP of Spindly-FLAG with recombinant USP45 WT showed a removal of the potential Spindly mono-ubiquitylation band (Fig.  xref , right panel)."
30258100
USP45 activates response to xenobiotic stimulus. 9 / 9
| 9
reach
"Further, we have discovered that increased USP45 expression significantly promoted cervical cancer cell proliferation, stemness and drug resistance, while USP45-enhanced MYC stability facilitated only stemness and drug resistance."
36765885
reach
"USP45 Promotes Cancer Proliferation, Stemness and Drug Resistance."
36765885
reach
"The cell viability and apoptosis analysis demonstrated that USP45 high expression enhanced drug resistance to doxorubicin (Figure 3H,I)."
36765885
reach
"In summary, we demonstrated that USP45 overexpression promoted cancer proliferation, stemness, drug resistance, and transformation of SiHa cells into CSC-like cells."
36765885
reach
"MYC Is a Downstream Effector in the USP45-Induced Cancer Stemness and Drug Resistance."
36765885
reach
"These results suggested that MYC was a critical downstream effector in USP45-induced cervical cancer stemness and drug resistance, but not proliferation."
36765885
reach
"We demonstrated that USP45 inhibition via destabilizing MYC suppressed the stemness and drug resistance of cervical cancer cells."
36765885
reach
"Alpha-Mangostin Inhibits the USP45-Induced Cancer Stemness, Drug Resistance and Tumorigenesis."
36765885
reach
"Suppression of Cancer Cell Stemness and Drug Resistance via MYC Destabilization by Deubiquitinase USP45 Inhibition with a Natural Small Molecule."
36765885
USP45 bound to natural small molecule inhibits response to xenobiotic stimulus. 2 / 2
| 2
reach
"Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (alpha-mangostin), in turn significantly suppressing the USP45-induced stemness and drug resistance of CSCs."
36765885
reach
"Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (alpha-mangostin), in turn significantly suppressing USP45-induced stemness and drug resistance."
36765885
USP45 affects CORO1B
| 8 2
USP45 binds CORO1B.
| 2 2
USP45 binds CORO1B. 4 / 4
| 2 2
reach
"USP45 interacted with and deubiquitinated the actin-regulatory protein Coronin 1B (Coro1B) to promote actin turnover."
40067150
reach
"Interestingly, coimmunoprecipitation (co-IP) assays showed that USP45 bound to Coro1B but not Coro1C via its N-terminal seven-bladed β-propeller domain (Fig. 5, A and B; and Fig. S3, A and B)."
40067150
sparser
"Given that a large fraction of USP45 is localized to endosomes and lysosomes, it is likely that the USP45–Coro1B axis imposes strict spatial and temporal control on N-WASP activity within the endo-lysosome system, thereby regulating V-ATPase localization and lysosomal function."
40067150
sparser
"Interestingly, coimmunoprecipitation (co-IP) assays showed that USP45 bound to Coro1B but not Coro1C via its N-terminal seven-bladed β-propeller domain ( xref ; and xref )."
40067150
USP45 deubiquitinates CORO1B.
| 3
USP45 deubiquitinates CORO1B. 3 / 3
| 3
reach
"USP45 interacted with and deubiquitinated the actin-regulatory protein Coronin 1B (Coro1B) to promote actin turnover."
40067150
reach
"Moreover, expression of USP45-WT, but not the catalytically inactive USP45 (C199A) mutant, dramatically decreased the ubiquitination of Coro1B under both mild (1% Triton) and denaturing (1% SDS) conditions (Fig. 5, K and L; and Fig. S3 D), suggesting that USP45 protease activity is required for the deubiquitination of Coro1B."
40067150
reach
"Since USP45 mediates Coro1B deubiquitination and regulates its stability, we investigated whether USP45 might regulate actin dynamics by targeting Coro1B."
40067150
USP45 ubiquitinates CORO1B.
| 1
USP45-C199A leads to the ubiquitination of CORO1B. 1 / 1
| 1
reach
"Moreover, expression of USP45-WT, but not the catalytically inactive USP45 (C199A) mutant, dramatically decreased the ubiquitination of Coro1B under both mild (1% Triton) and denaturing (1% SDS) conditions (Fig. 5, K and L; and Fig. S3 D), suggesting that USP45 protease activity is required for the deubiquitination of Coro1B."
40067150
USP45 decreases the amount of CORO1B.
| 1
USP45 decreases the amount of ubiquitinated CORO1B. 1 / 1
| 1
reach
"The in vivo ubiquitination assay showed that USP45 depletion led to increased levels of ubiquitinated Coro1B (Fig. 5, I and J)."
40067150
USP45 activates CORO1B.
| 1
USP45 activates CORO1B. 1 / 1
| 1
reach
"Furthermore, the cycloheximide-chase assay revealed that USP45 depletion enhanced the protein degradation of Coro1B (Fig. 5, G and H)."
40067150
Bisphenol A affects USP45
6 |
Bisphenol A decreases the amount of USP45.
3 |
Bisphenol A decreases the amount of USP45. 3 / 3
3 |
ctd
No evidence text available
34947998
ctd
No evidence text available
30816183
ctd
No evidence text available
35504463
Bisphenol A methylates USP45.
1 |
Bisphenol A methylates USP45. 1 / 1
1 |
ctd
No evidence text available
28505145
Bisphenol A increases the amount of USP45.
1 |
Bisphenol A increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
25181051
Bisphenol A demethylates USP45.
1 |
Bisphenol A demethylates USP45. 1 / 1
1 |
ctd
No evidence text available
30906313
USP45 affects localization
| 6
USP45 activates localization.
| 4
USP45 activates localization. 4 / 4
| 4
reach
"Interestingly, the ablation of USP45 significantly enhanced the localization of the V-ATPase V0 subunit d (ATP6V0D) at late endosomes (Rab 7) and lysosomes (LAMP2), while having no effects on its localization at early endosomes (EEA1) (Fig. 4, E and F)."
40067150
reach
"We then investigated whether USP45 could modulate the localization of V-ATPase on endolysosomes."
40067150
reach
"dUsp45/USP45 impairs lysosomal acidification and disrupts the localization of V-ATPases to lysosomes by modulating actin structures."
40067150
reach
"The Usp45 secretion and spaX (S. aureus protein A) anchor signals efficiently promote the localization of FNIII 7-10 in the bacterial membrane."
25068919
USP45 inhibits localization.
| 2
USP45 inhibits localization. 2 / 2
| 2
reach
"We found that knockdown of USP45 dramatically enhanced the localization of V-ATPase to late endosomes and lysosomes, thereby enhancing the number of acidified lysosomes and lysosomal function.Accumulating evidence has highlighted the pivotal roles of ubiquitination and deubiquitination in governing endocytic trafficking (Piper et al., 2014)."
40067150
reach
"Further, we showed that depletion of dUsp45/USP45 promotes V-ATPase localization to autolysosomes, thereby enhancing lysosomal acidification and autophagic–lysosomal activity."
40067150
reach
"Based on the results of cell clone formation assays and CCK-8 assays, overexpression of USP45 significantly promoted the proliferation of KYSE140 and KYSE410 cells (Fig. 1B, C), whereas knockdown of USP45 inhibited cell proliferation (Fig. 1D, E)."
38868575
reach
"USP45 Promotes Cancer Proliferation, Stemness and Drug Resistance."
36765885
reach
"In addition, the colony formation and proliferation of SiHa cells were significantly increased by USP45 overexpression (Figure 3D,E)."
36765885
reach
"In summary, we demonstrated that USP45 overexpression promoted cancer proliferation, stemness, drug resistance, and transformation of SiHa cells into CSC-like cells."
36765885
reach
"In the present study, we demonstrated that the expression level of USP45 in the majority types of tumors was positively correlated with the tumor stemness index, suggesting that upregulation of USP45 may promote the proliferation and metastasis of tumor cells."
35836933
USP45 inhibits cell population proliferation. 1 / 1
| 1
reach
"Based on the results of cell clone formation assays and CCK-8 assays, overexpression of USP45 significantly promoted the proliferation of KYSE140 and KYSE410 cells (Fig. 1B, C), whereas knockdown of USP45 inhibited cell proliferation (Fig. 1D, E)."
38868575
USP45 affects autophagy
| 6
USP45 inhibits autophagy.
| 5
USP45 inhibits autophagy. 5 / 5
| 5
reach
"USP45 negatively regulates autophagy and lysosomal activity."
40067150
reach
"Correction: The deubiquitinase USP45 inhibits autophagy through actin regulation by Coronin 1B."
40184486
reach
"Mammalian USP45 negatively regulates autophagy."
40184486
reach
"These results suggest that, similar to its Drosophila counterpart, mammalian USP45 also negatively regulates autophagosome formation and autophagy flux.To investigate the mechanism by which USP45 activates autophagy, we examined the effects of USP45 depletion on the expression of key autophagy-regulating proteins."
40067150
reach
"The deubiquitinase USP45 inhibits autophagy through actin regulation by Coronin 1B."
40067150
USP45 activates autophagy.
| 1
USP45 activates autophagy. 1 / 1
| 1
reach
"These results suggest that, similar to its Drosophila counterpart, mammalian USP45 also negatively regulates autophagosome formation and autophagy flux.To investigate the mechanism by which USP45 activates autophagy, we examined the effects of USP45 depletion on the expression of key autophagy-regulating proteins."
40067150
USP45 affects ERCC4
2 | 4
USP45 binds ERCC4 and ERCC1. 4 / 4
| 4
sparser
"USP45 associates with the ERCC1–XPF endonuclease."
25538220
sparser
"Upon UV exposure, USP45 interacts with ERCC1–XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that cannot bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
sparser
"These data suggested that USP45 interacts directly with XPF–ERCC1 and not with SLX4."
25538220
sparser
"USP45 interacts specifically with ERCC1–XPF via its N-terminal 61 residues."
25538220
USP45 binds ERCC4. 2 / 2
2 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
25538220
USP45 affects V-ATPase
| 5
USP45 inhibits V-ATPase.
| 3
USP45 inhibits V-ATPase. 3 / 3
| 3
reach
"We found that knockdown of USP45 dramatically enhanced the localization of V-ATPase to late endosomes and lysosomes, thereby enhancing the number of acidified lysosomes and lysosomal function.Accumulating evidence has highlighted the pivotal roles of ubiquitination and deubiquitination in governing endocytic trafficking (Piper et al., 2014)."
40067150
reach
"USP45 negatively regulates lysosomal activity by altering V-ATPase endolysosomal trafficking."
40184486
reach
"Further, we showed that depletion of dUsp45/USP45 promotes V-ATPase localization to autolysosomes, thereby enhancing lysosomal acidification and autophagic–lysosomal activity."
40067150
USP45 activates V-ATPase.
| 2
USP45 activates V-ATPase. 2 / 2
| 2
reach
"We then investigated whether USP45 could modulate the localization of V-ATPase on endolysosomes."
40067150
reach
"Interestingly, the ablation of USP45 significantly enhanced the localization of the V-ATPase V0 subunit d (ATP6V0D) at late endosomes (Rab 7) and lysosomes (LAMP2), while having no effects on its localization at early endosomes (EEA1) (Fig. 4, E and F)."
40067150
USP45 activates Neoplastic Stem Cells.
| 3
reach
"To investigate the causative roles of MYC in the proliferation, stemness and drug resistance of the USP45-induced CSC-like SiHa cells, we performed MYC deletion."
36765885
reach
"Since the USP45-induced CSC-like SiHa cells have strong capacities in tumorigenesis (Figure 7A) and drug resistance, their suppressions were investigated with AMG, DOX and their combination."
36765885
reach
"In summary, we demonstrated that USP45 overexpression promoted cancer proliferation, stemness, drug resistance, and transformation of SiHa cells into CSC-like cells."
36765885
reach
"With AMG, the tumorsphere formation, CD133+ cells population and Hoechst 33,342 efflux of USP45-induced CSC-like SiHa cells were significantly inhibited (Figure 6B–E)."
36765885
USP45 increases the amount of Neoplastic Stem Cells.
| 1
USP45 increases the amount of Neoplastic Stem Cells. 1 / 1
| 1
reach
"The USP45/MYC axis elevates the expression of MYC-targeted downstream factors and CSC-associated proteins, leading to an increase in tumorsphere formation and CD133+ cell populations [95]."
38702734
USP45 affects SLX4
2 | 1
USP45 binds SLX4. 3 / 4
2 | 1
biogrid
No evidence text available
25538220
sparser
"In agreement with USP45 associating with SLX4 indirectly through its ability to bind ERCC1, we found that USP45 did not co-immunoprecipitate with SLX4 from ERCC1 knockout mouse embryonic fibroblasts (MEFs) (Fig xref D; McWhir et al , xref ; Melton et al , xref )."
25538220
biogrid
No evidence text available
25538220
USP45 affects F_actin
| 3 1
USP45 inhibits F_actin.
| 3
USP45 inhibits F_actin. 3 / 3
| 3
reach
"We next examined whether the F-actin patches induced by USP45 depletion were required for the translocalization of V-ATPase to lysosomes."
40067150
reach
"The F-actin patches induced by USP45 knockdown highly colocalized with the late endosomal marker Rab7 and the lysosomal marker LAMP1, but not with the early endosomal marker EEA1 (Fig. 6, C and D), suggesting a role for cytosolic F-actin patches in regulating endolysosomal activity."
40067150
reach
"Indeed, the F-actin patches induced by USP45 depletion showed high colocalization with LysoTracker-positive acidic lysosomes (Fig. S4 A)."
40067150
USP45 binds F_actin.
| 1
USP45 binds F_actin. 1 / 1
| 1
sparser
"USP45 depletion–induced F-actin patch formation is crucial for V-ATPase lysosome localization and lysosomal acidification."
40067150
SLX4 affects USP45
2 | 1
USP45 binds SLX4. 3 / 4
2 | 1
biogrid
No evidence text available
25538220
sparser
"In agreement with USP45 associating with SLX4 indirectly through its ability to bind ERCC1, we found that USP45 did not co-immunoprecipitate with SLX4 from ERCC1 knockout mouse embryonic fibroblasts (MEFs) (Fig xref D; McWhir et al , xref ; Melton et al , xref )."
25538220
biogrid
No evidence text available
25538220
ERCC4 affects ERCC1
| 4
USP45 binds ERCC4 and ERCC1. 4 / 4
| 4
sparser
"USP45 associates with the ERCC1–XPF endonuclease."
25538220
sparser
"Upon UV exposure, USP45 interacts with ERCC1–XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that cannot bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
sparser
"These data suggested that USP45 interacts directly with XPF–ERCC1 and not with SLX4."
25538220
sparser
"USP45 interacts specifically with ERCC1–XPF via its N-terminal 61 residues."
25538220
CORO1B affects USP45
| 2 2
USP45 binds CORO1B. 4 / 4
| 2 2
reach
"USP45 interacted with and deubiquitinated the actin-regulatory protein Coronin 1B (Coro1B) to promote actin turnover."
40067150
reach
"Interestingly, coimmunoprecipitation (co-IP) assays showed that USP45 bound to Coro1B but not Coro1C via its N-terminal seven-bladed β-propeller domain (Fig. 5, A and B; and Fig. S3, A and B)."
40067150
sparser
"Given that a large fraction of USP45 is localized to endosomes and lysosomes, it is likely that the USP45–Coro1B axis imposes strict spatial and temporal control on N-WASP activity within the endo-lysosome system, thereby regulating V-ATPase localization and lysosomal function."
40067150
sparser
"Interestingly, coimmunoprecipitation (co-IP) assays showed that USP45 bound to Coro1B but not Coro1C via its N-terminal seven-bladed β-propeller domain ( xref ; and xref )."
40067150
Air Pollutants affects USP45
4 |
Air Pollutants increases the amount of USP45.
3 |
Air Pollutants increases the amount of USP45. 3 / 3
3 |
ctd
No evidence text available
38245732
ctd
No evidence text available
37586242
ctd
No evidence text available
32699268
Air Pollutants decreases the amount of USP45.
1 |
Air Pollutants decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
37586242
Valproic acid affects USP45
3 |
Valproic acid increases the amount of USP45.
2 |
Valproic acid increases the amount of USP45. 2 / 2
2 |
ctd
No evidence text available
29154799
ctd
No evidence text available
24935251
Valproic acid demethylates USP45.
1 |
Valproic acid demethylates USP45. 1 / 1
1 |
ctd
No evidence text available
29154799
Folic acid affects USP45
3 |
Folic acid decreases the amount of USP45.
2 |
Folic acid decreases the amount of USP45. 2 / 2
2 |
ctd
No evidence text available
25629700
ctd
No evidence text available
20938992
Folic acid increases the amount of USP45.
1 |
Folic acid increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
22206623
Alpha-mangostin affects USP45
| 3
Alpha-mangostin inhibits USP45. 3 / 3
| 3
reach
"Alpha-Mangostin Is an Inhibitor of USP45."
36765885
reach
"Alpha-Mangostin Inhibits the USP45-Induced Cancer Stemness, Drug Resistance and Tumorigenesis."
36765885
reach
"Since USP45 is significantly expressed in cervical tumors, we have discovered that the combination of alpha-mangostin and doxorubicin can significantly inhibit USP45-induced cervical tumorigenesis in an animal model."
36765885
USP45 affects SNAI1
| 3
USP45 deubiquitinates SNAI1.
| 2
USP45 deubiquitinates SNAI1. 2 / 2
| 2
reach
"Further, we observed that MYH9 could interact with USP45, snail, and ubiquitin with endogenous Co‐IP assays, respectively (Figure 5C), and the CoIP assay demonstrated that USP45 knockdown ameliorated the effect of MYH9 on the deubiquitination and stability of snail (Figure 5D)."
36929633
reach
"Subsequent studies reveal that, USP45, recruited by MYH9 and MYH10, deubiquitinates Snail in serous ovarian cancer (SOC) [65]."
38702734
USP45 activates SNAI1.
| 1
USP45 activates SNAI1. 1 / 1
| 1
reach
"In addition, we found that USP45 knockdown could impair the stability of snail in SOC cells treated with cycloheximide and MG132 at different time points (Figure 5I,J).2.6 Promotion of MYH10 on SOC Carcinogenesis, Progression, and Cisplatin Resistance could be Reversed by Knockdown of MYH9."
36929633
USP45 affects PCNA
| 3
USP45 increases the amount of PCNA.
| 2
USP45 increases the amount of PCNA. 2 / 2
| 2
reach
"USP45 knockdown significantly inhibited the growth rate and weight of tumors and the protein expression of cyclin D1, Ki-67, and PCNA in nude mice (Fig. 1I)."
38868575
reach
"Furthermore, the effect of USP45 on cell proliferation was also confirmed by Western blot, which showed that overexpression of USP45 up-regulated the expression of cyclin D1, Ki-67, and PCNA (Fig. 1F), while knockdown of USP45 down-regulated cyclin D1, Ki-67, and PCNA (Fig. 1G)."
38868575
USP45 activates PCNA.
| 1
USP45 activates PCNA. 1 / 1
| 1
reach
"Furthermore, the effect of USP45 on cell proliferation was also confirmed by Western blot, which showed that overexpression of USP45 up-regulated the expression of cyclin D1, Ki-67, and PCNA (Fig. 1F), while knockdown of USP45 down-regulated cyclin D1, Ki-67, and PCNA (Fig. 1G)."
38868575
USP45 affects MYH9
3 |
USP45 binds MYH9. 3 / 3
3 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36929633
USP45 affects MYH10
3 |
USP45 binds MYH10. 3 / 3
3 |
biogrid
No evidence text available
36929633
biogrid
No evidence text available
19615732
biogrid
No evidence text available
25538220
USP45 affects MKI67
| 3
USP45 increases the amount of MKI67.
| 2
USP45 increases the amount of MKI67. 2 / 2
| 2
reach
"USP45 knockdown significantly inhibited the growth rate and weight of tumors and the protein expression of cyclin D1, Ki-67, and PCNA in nude mice (Fig. 1I)."
38868575
reach
"Furthermore, the effect of USP45 on cell proliferation was also confirmed by Western blot, which showed that overexpression of USP45 up-regulated the expression of cyclin D1, Ki-67, and PCNA (Fig. 1F), while knockdown of USP45 down-regulated cyclin D1, Ki-67, and PCNA (Fig. 1G)."
38868575
USP45 activates MKI67.
| 1
USP45 activates MKI67. 1 / 1
| 1
reach
"Furthermore, the effect of USP45 on cell proliferation was also confirmed by Western blot, which showed that overexpression of USP45 up-regulated the expression of cyclin D1, Ki-67, and PCNA (Fig. 1F), while knockdown of USP45 down-regulated cyclin D1, Ki-67, and PCNA (Fig. 1G)."
38868575
USP45 affects FBXL7
| 1 1
FBXL7 binds USP45. 1 / 2
| 1
reach
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12."
25654763
FBXL7 binds USP1 and USP45. 1 / 1
| 1
sparser
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12. xref These deubiquitylation enzymes could counteract SCF Fbxl18 to remove ubiquitin from Fbxl7 to prevent its degradation."
25654763
USP45 affects Actin
| 3
USP45 inhibits Actin.
| 1
USP45 bound to CORO1B inhibits Actin. 1 / 1
| 1
reach
"USP45 interacted with and deubiquitinated the actin-regulatory protein Coronin 1B (Coro1B) to promote actin turnover."
40067150
USP45 deubiquitinates Actin.
| 1
USP45 deubiquitinates Actin. 1 / 1
| 1
reach
"Firstly, USP45 interacts with and deubiquitinates the actin-associated protein Coro1B, thereby stabilizing its protein levels."
40067150
USP45 binds Actin.
| 1
USP45 binds Actin. 1 / 1
| 1
reach
"Firstly, USP45 interacts with and deubiquitinates the actin-associated protein Coro1B, thereby stabilizing its protein levels."
40067150
MYH9 affects USP45
3 |
USP45 binds MYH9. 3 / 3
3 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
19615732
biogrid
No evidence text available
36929633
MYH10 affects USP45
3 |
USP45 binds MYH10. 3 / 3
3 |
biogrid
No evidence text available
36929633
biogrid
No evidence text available
19615732
biogrid
No evidence text available
25538220
FBXL7 affects USP45
| 1 1
FBXL7 binds USP45. 1 / 2
| 1
reach
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12."
25654763
FBXL7 binds USP1 and USP45. 1 / 1
| 1
sparser
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12. xref These deubiquitylation enzymes could counteract SCF Fbxl18 to remove ubiquitin from Fbxl7 to prevent its degradation."
25654763
Vinclozolin affects USP45
2 |
Vinclozolin methylates USP45.
1 |
Vinclozolin methylates USP45. 1 / 1
1 |
ctd
No evidence text available
31682807
Vinclozolin increases the amount of USP45.
1 |
Vinclozolin increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
23034163
Paracetamol affects USP45
2 |
Paracetamol increases the amount of USP45. 2 / 2
2 |
ctd
No evidence text available
26690555
ctd
No evidence text available
33387578
Natural small molecule affects USP45
| 2
USP45 binds natural small molecule. 2 / 2
| 2
reach
"Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (alpha-mangostin), in turn significantly suppressing the USP45-induced stemness and drug resistance of CSCs."
36765885
reach
"Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (alpha-mangostin), in turn significantly suppressing USP45-induced stemness and drug resistance."
36765885
Doxorubicin affects USP45
1 | 1
Doxorubicin inhibits USP45.
| 1
Doxorubicin inhibits USP45. 1 / 1
| 1
reach
"Since USP45 is significantly expressed in cervical tumors, we have discovered that the combination of alpha-mangostin and doxorubicin can significantly inhibit USP45-induced cervical tumorigenesis in an animal model."
36765885
Doxorubicin decreases the amount of USP45.
1 |
Doxorubicin decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
29803840
Dioxygen affects USP45
2 |
Dioxygen increases the amount of USP45.
1 |
Dioxygen increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
22629407
Dioxygen decreases the amount of USP45.
1 |
Dioxygen decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
22629407
Bortezomib affects USP45
1 | 1
Bortezomib increases the amount of USP45.
1 |
Bortezomib increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
20977926
Bortezomib activates USP45.
| 1
Bortezomib activates USP45. 1 / 1
| 1
reach
"In particular, we found that ZOL inhibited expression of the ubiquitin-specific peptidase 24 ( USP24) a de-ubiquitin protease that is overexpressed in bortezomib-resistant RPMI-R5 cells compared to RP[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20977926
Beta-lapachone affects USP45
2 |
Beta-lapachone increases the amount of USP45.
1 |
Beta-lapachone increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
38218311
Beta-lapachone decreases the amount of USP45.
1 |
Beta-lapachone decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
38218311
Aflatoxin B1 affects USP45
2 |
Aflatoxin B1 methylates USP45.
1 |
Aflatoxin B1 methylates USP45. 1 / 1
1 |
ctd
No evidence text available
30157460
Aflatoxin B1 increases the amount of USP45.
1 |
Aflatoxin B1 increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
19770486
ZFR affects USP45
2 |
USP45 binds ZFR. 2 / 2
2 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
19615732
USP45 affects pH reduction
| 2
USP45 inhibits pH reduction. 2 / 2
| 2
reach
"Further, we showed that depletion of dUsp45/USP45 promotes V-ATPase localization to autolysosomes, thereby enhancing lysosomal acidification and autophagic–lysosomal activity."
40067150
reach
"dUsp45/USP45 impairs lysosomal acidification and disrupts the localization of V-ATPases to lysosomes by modulating actin structures."
40067150
USP45 affects natural small molecule
| 2
USP45 binds natural small molecule. 2 / 2
| 2
reach
"Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (alpha-mangostin), in turn significantly suppressing the USP45-induced stemness and drug resistance of CSCs."
36765885
reach
"Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (alpha-mangostin), in turn significantly suppressing USP45-induced stemness and drug resistance."
36765885
USP45 affects ZFR
2 |
USP45 binds ZFR. 2 / 2
2 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
19615732
USP45 affects RBMX
2 |
USP45 binds RBMX. 2 / 2
2 |
biogrid
No evidence text available
19615732
biogrid
No evidence text available
25538220
USP45 affects MYL12B
2 |
USP45 binds MYL12B. 2 / 2
2 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
19615732
USP45 affects ERCC1-XPF
| 2
USP45 binds ERCC1-XPF. 2 / 2
| 2
reach
"Upon UV exposure, USP45 interacts with ERCC1-XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that can not bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
reach
"USP45 interacts specifically with ERCC1-XPF via its N-terminal 61 residues."
25538220
USP45 affects Cyclin
| 2
USP45 increases the amount of Cyclin.
| 1
USP45 increases the amount of Cyclin. 1 / 1
| 1
reach
"Furthermore, the effect of USP45 on cell proliferation was also confirmed by Western blot, which showed that overexpression of USP45 up-regulated the expression of cyclin D1, Ki-67, and PCNA (Fig. 1F), while knockdown of USP45 down-regulated cyclin D1, Ki-67, and PCNA (Fig. 1G)."
38868575
USP45 activates Cyclin.
| 1
USP45 activates Cyclin. 1 / 1
| 1
reach
"Furthermore, the effect of USP45 on cell proliferation was also confirmed by Western blot, which showed that overexpression of USP45 up-regulated the expression of cyclin D1, Ki-67, and PCNA (Fig. 1F), while knockdown of USP45 down-regulated cyclin D1, Ki-67, and PCNA (Fig. 1G)."
38868575
USP45 affects Carcinogenesis
| 2
USP45 activates Carcinogenesis. 2 / 2
| 2
reach
"Since USP45 is significantly expressed in cervical tumors, we have discovered that the combination of alpha-mangostin and doxorubicin can significantly inhibit USP45-induced cervical tumorigenesis in an animal model."
36765885
reach
"Alpha-Mangostin Inhibits the USP45-Induced Cancer Stemness, Drug Resistance and Tumorigenesis."
36765885
USP45 affects ATAD2
| 2
USP45 increases the amount of ATAD2.
| 1
USP45 increases the amount of ATAD2. 1 / 1
| 1
reach
"Furthermore, through RT-qPCR and Western blot, we confirm that USP45 overexpression up-regulated the mRNA and protein levels of MYC and ATAD2, while USP45 knockdown down-regulated MYC and ATAD2."
38868575
USP45 activates ATAD2.
| 1
USP45 activates ATAD2. 1 / 1
| 1
reach
"Furthermore, through RT-qPCR and Western blot, we confirm that USP45 overexpression up-regulated the mRNA and protein levels of MYC and ATAD2, while USP45 knockdown down-regulated MYC and ATAD2."
38868575
RBMX affects USP45
2 |
USP45 binds RBMX. 2 / 2
2 |
biogrid
No evidence text available
19615732
biogrid
No evidence text available
25538220
Particulate Matter increases the amount of USP45.
1 |
Particulate Matter increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
37586242
Particulate Matter decreases the amount of USP45.
1 |
Particulate Matter decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
37586242
MYL12B affects USP45
2 |
USP45 binds MYL12B. 2 / 2
2 |
biogrid
No evidence text available
25538220
biogrid
No evidence text available
19615732
ERCC1-XPF affects USP45
| 2
USP45 binds ERCC1-XPF. 2 / 2
| 2
reach
"Upon UV exposure, USP45 interacts with ERCC1-XPF but the USP45 mutant (Asp25Ala, Glu26Ala) that is catalytically active but that can not bind to ERCC1 failed to deubiquitinate ERCC1."
27827925
reach
"USP45 interacts specifically with ERCC1-XPF via its N-terminal 61 residues."
25538220
AMELX affects USP45
| 1 1
AMELX inhibits USP45. 2 / 2
| 1 1
sparser
"Via our structural and functional studies, we have found that AMG can directly inhibit USP45 (with Kd of 19.3 μM), causing MYC destabilization and, in turn, suppressing the stemness and drug resistance of cervical CSCs."
36765885
reach
"Via our structural and functional studies, we have found that AMG can directly inhibit USP45 (with Kd of 19.3 μM), causing MYC destabilization and, in turn, suppressing the stemness and drug resistance of cervical CSCs."
36765885
2,3,7,8-tetrachlorodibenzodioxine increases the amount of USP45.
1 |
2,3,7,8-tetrachlorodibenzodioxine increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
32109520
2,3,7,8-tetrachlorodibenzodioxine decreases the amount of USP45.
1 |
2,3,7,8-tetrachlorodibenzodioxine decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
33387578
17alpha-ethynylestradiol increases the amount of USP45.
1 |
17alpha-ethynylestradiol increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
29097150
17alpha-ethynylestradiol decreases the amount of USP45.
1 |
17alpha-ethynylestradiol decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
23129252
Vorinostat affects USP45
1 |
Vorinostat increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
27188386
ctd
No evidence text available
26179874
Trichostatin A affects USP45
1 |
Trichostatin A increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
24935251
Titanium dioxide affects USP45
1 |
Titanium dioxide demethylates USP45. 1 / 1
1 |
ctd
No evidence text available
35295148
Thioacetamide affects USP45
1 |
Thioacetamide decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
34492290
Sunitinib affects USP45
1 |
Sunitinib increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
31533062
Sorafenib affects USP45
1 |
Sorafenib decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
39786591
Signal peptide affects USP45
| 1
Signal peptide binds USP45. 1 / 1
| 1
sparser
"In L. lactis , the Usp45-signal peptide is the most widely used signal peptide (SP) for the secretion of heterologous proteins [ xref ]."
36286175
Rotenone affects USP45
1 |
Rotenone decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
39786591
Rosiglitazone affects USP45
1 |
Rosiglitazone decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
35504463
PstIR affects USP45
| 1
PstIR activates USP45. 1 / 1
| 1
reach
"The psaA gene was cloned into the pT1NX vector PstI site, which produced a fusion of a truncated usp45 signal peptide carried by the vector to the PsaA sequence (Fig. 1), under the control of a constitutive promoter."
16549380
Pirinixic acid affects USP45
1 |
Pirinixic acid decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
18445702
Phorbol 13-acetate 12-myristate increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
25613284
Phlorizin affects USP45
1 |
Phlorizin decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
22538082
Perfluorooctane-1-sulfonic acid decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
36331819
Ozone affects USP45
1 |
Ozone increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
32699268
Mono(2-ethylhexyl) phthalate decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
31059758
Mitomycin C affects USP45
| 1
Mitomycin C activates USP45. 1 / 1
| 1
reach
"We also explored how USP45 knockout impacts on gamma-H2AX foci induced by MMC."
25538220
Methylmercury chloride decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
28001369
Methyl methanesulfonate increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
23649840
Methapyrilene affects USP45
1 |
Methapyrilene methylates USP45. 1 / 1
1 |
ctd
No evidence text available
30157460
Methacrylaldehyde affects USP45
1 |
Methacrylaldehyde increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
32699268
Manganese(II) chloride decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
17175027
Manganese atom affects USP45
1 |
Manganese atom decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
17175027
Lactacystin affects USP45
| 1
Lactacystin inhibits USP45. 1 / 1
| 1
reach
"To explore whether USP45 can directly catalyse deubiquitylation of ERCC1, we isolated ubiquitylated Flag-ERCC1 (Fig XREF_FIG C) or GFP-ERCC1 (XREF_SUPPLEMENTARY), expressed in USP45 knockout U2OS cells treated with lactacystin proteasomal inhibitor."
25538220
Ionomycin affects USP45
1 |
Ionomycin increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
25613284
Hsa-mir-3662 affects USP45
1 |
Hsa-mir-3662 decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-mir-3149 affects USP45
1 |
Hsa-mir-3149 decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-mir-3148 affects USP45
1 |
Hsa-mir-3148 decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-6873-5p affects USP45
1 |
Hsa-miR-6873-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-6783-5p affects USP45
1 |
Hsa-miR-6783-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-526a affects USP45
1 |
Hsa-miR-526a decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-523-5p affects USP45
1 |
Hsa-miR-523-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-522-5p affects USP45
1 |
Hsa-miR-522-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-520g-5p affects USP45
1 |
Hsa-miR-520g-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-520c-5p affects USP45
1 |
Hsa-miR-520c-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-519c-5p affects USP45
1 |
Hsa-miR-519c-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-519b-5p affects USP45
1 |
Hsa-miR-519b-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-519a-5p affects USP45
1 |
Hsa-miR-519a-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-518f-5p affects USP45
1 |
Hsa-miR-518f-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-518e-5p affects USP45
1 |
Hsa-miR-518e-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-518d-5p affects USP45
1 |
Hsa-miR-518d-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-4777-5p affects USP45
1 |
Hsa-miR-4777-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-4668-5p affects USP45
1 |
Hsa-miR-4668-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-32-5p affects USP45
1 |
Hsa-miR-32-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-3145-3p affects USP45
1 |
Hsa-miR-3145-3p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Hsa-miR-192-5p affects USP45
1 |
Hsa-miR-192-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
19074876
Hsa-let-7f-5p affects USP45
1 |
Hsa-let-7f-5p decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23622248
Gallic acid affects USP45
1 |
Gallic acid decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
34408198
Galactose affects USP45
| 1
Galactose activates USP45. 1 / 1
| 1
sparser
"We also show that the usp45 promoter is highly activated by galactose."
32532874
Formaldehyde affects USP45
1 |
Formaldehyde decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
23649840
Dexamethasone affects USP45
1 |
Dexamethasone decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
35504463
Crocidolite asbestos decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
29279043
Choline affects USP45
1 |
Choline decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
20938992
Chlorpyrifos affects USP45
1 |
Chlorpyrifos decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
37019170
Cadmium dichloride demethylates USP45. 1 / 1
1 |
ctd
No evidence text available
22457795
Biotin affects USP45
1 |
Biotin decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
35504463
Beta-D-glucose affects USP45
1 |
Beta-D-glucose decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
36331819
Benzo[e]pyrene affects USP45
1 |
Benzo[e]pyrene methylates USP45. 1 / 1
1 |
ctd
No evidence text available
30157460
Alpha-pinene affects USP45
1 |
Alpha-pinene increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
32699268
Alpha-D-galactose affects USP45
| 1
Alpha-D-galactose activates USP45. 1 / 1
| 1
reach
"We also show that the usp45 promoter is highly activated by galactose."
32532874
All-trans-retinoic acid decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
33167477
Acrolein affects USP45
1 |
Acrolein increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
32699268
YLPM1 affects USP45
1 |
USP45 binds YLPM1. 1 / 1
1 |
biogrid
No evidence text available
19615732
XPF-ERCC1 affects USP45
| 1
XPF-ERCC1 activates USP45. 1 / 1
| 1
reach
"On this basis, we speculate that is likely that XPF-ERCC1 is the major target of USP45 in controlling DNA repair."
25538220
WIZ affects USP45
1 |
WIZ binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45
1 |
USP45 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
33864866
USP45 affects β-1,3-1,4-glucanase
| 1
USP45 increases the amount of β-1,3-1,4-glucanase. 1 / 1
| 1
reach
"Usp45 was fused with the BG gene to promote extracellular secretion of β-1,3-1,4-glucanase."
31112758
USP45 affects ubiquitin-proteasome
| 1
USP45 inhibits ubiquitin-proteasome. 1 / 1
| 1
reach
"It was shown that USP45 could inhibit the ubiquitin-proteasome degradation pathway of ERCC1 protein, a DNA repair-related nucleic acid endonuclease, thereby enhancing the ability of tumor cells to repair DNA damage.4 KEGG pathway analysis showed that USP45 may be associated with the MAPK signaling pathway, AMPK signaling pathway, Hippo signaling pathway, cell cycle, p53 signaling pathway, and TGF-β signaling pathway (Fig. 1N and Table S5)."
38868575
reach
"In this study, USP7, USP10, USP13, USP18, USP45, UCHL1 and SENP2 were all found to reduce myogenesis and may therefore all be essential for the switch from proliferation to differentiation [19]."
38716888
USP45 affects signal peptide
| 1
Signal peptide binds USP45. 1 / 1
| 1
sparser
"In L. lactis , the Usp45-signal peptide is the most widely used signal peptide (SP) for the secretion of heterologous proteins [ xref ]."
36286175
USP45 affects secretion heterologous protein L. lactis
| 1
USP45 activates secretion heterologous protein L. lactis. 1 / 1
| 1
eidos
"One report concluded that usp45 could enhance secretion to increase heterologous protein production in L. lactis [ 21 ] ."
31015862
USP45 affects sacA
| 1
USP45 activates sacA. 1 / 1
| 1
reach
"Removal of the USP45 secretion peptide allowed a soluble and functional invertase to be expressed intracellularly in L. lactis."
28155177
USP45 affects recombinant GarQ
| 1
USP45 activates recombinant GarQ. 1 / 1
| 1
reach
"In fact, it was shown for several bacteriocins that the double glycine SPs can be successfully replaced by Sec-dependent SPs (McCormick et al., 1996; Martín et al., 2007; Borrero et al., 2011; Back et al., 2016) and recently a fusion of the SP of lactococcal protein Usp45 and GarQ allowed recombinant GarQ production by L. lactis (Feito et al., 2023)."
38260893
USP45 affects proteolysis
| 1
USP45 activates proteolysis. 1 / 1
| 1
reach
"Furthermore, the cycloheximide-chase assay revealed that USP45 depletion enhanced the protein degradation of Coro1B (Fig. 5, G and H)."
40067150
USP45 affects protein secretion
| 1
reach
"The standard substances were glucose, lactose, cellotriose, cellotetrose, 3-O-β-cellobiosyl- d -glucose, 3-O-β-cellotriosyl- d -glucose, and β-glucans.The pSEC:Nuc plasmid contains Usp45 , which enabl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31112758
reach
"On the other hand, Usp45, as the original signal peptide of L. lactis , is adapted to the secretion mechanism of L. lactis and can sufficiently induce enzyme protein localization [16,39–41] ."
36608408
USP45 affects plasmin
| 1
USP45 increases the amount of plasmin. 1 / 1
| 1
reach
"Expression of the plasmin gene driven by the promoter and complete signal sequence of the lactococcal usp45 gene resulted in efficient plasmin secretion in strain BPL420."
9352676
USP45 affects peptide
| 1
USP45 activates peptide. 1 / 1
| 1
reach
"Analysis of the amino acid sequences performed on the two hsTRAIL protein bands showed that the upper (larger) band represents a slightly truncated form of hsTRAIL containing 160 amino acid (aa) residues, while the lower (smaller) band represents a more truncated form of hsTRAIL deprived of 37 aa residues from the N-terminus of the protein, both having the bacterial usp45 leading peptide cut off."
30446013
USP45 affects pathway
| 1
USP45 inhibits pathway. 1 / 1
| 1
sparser
"It was shown that USP45 could inhibit the ubiquitin-proteasome degradation pathway of ERCC1 protein, a DNA repair-related nucleic acid endonuclease, thereby enhancing the ability of tumor cells to repair DNA damage. xref KEGG pathway analysis showed that USP45 may be associated with the MAPK signaling pathway, AMPK signaling pathway, Hippo signaling pathway, cell cycle, p53 signaling pathway, and TGF-β signaling pathway ( xref N and xref )."
38868575
USP45 affects nisin
| 1
USP45 activates nisin. 1 / 1
| 1
reach
"As shown in Fig. 3 , when P nisA promoter and signal peptides (sp Usp45 ) were used to direct the secretion of barnase using 5 ng/mL nisin, after 36 h fermentation the strain secreted the highest yiel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36608408
USP45 affects migration velocity
| 1
USP45 activates migration velocity. 1 / 1
| 1
eidos
"Our data indicate that the lack of USP45 reduces migration velocity compared to wild-type cells ."
30258100
USP45 affects miR-30e-5p
| 1
USP45 activates miR-30e-5p. 1 / 1
| 1
reach
"Furthermore, Usp45 is also potential target of miR-30e-5p which is a regulator of XPF-ERCC1 crucial for efficient DNA repair [XREF_BIBR]."
26439850
USP45 affects doxorubicin
| 1
USP45 activates doxorubicin. 1 / 1
| 1
reach
"The cell viability and apoptosis analysis demonstrated that USP45 high expression enhanced drug resistance to doxorubicin (Figure 3H,I)."
36765885
USP45 affects dimer
| 1
USP45 activates dimer. 1 / 1
| 1
reach
"Fig. 2D indicate that recombinant USP45 WT is able to mediate the cleavage of 100% of the K6, K48 and K63 dimer in 60 min."
30258100
USP45 affects cell migration
| 1
reach
"USP45 depleted cells were defective in cell migration and the defects observed were not produced by off-target effects: phenotypic rescue could be achieved only by re-expressing the catalytically active USP45 and not with the inactive mutant C199A, suggesting a potential interplay of Spindly and USP45 in cell migration (Fig. 5C)."
30258100
USP45 inhibits autophagosome assembly. 1 / 1
| 1
reach
"These results suggest that, similar to its Drosophila counterpart, mammalian USP45 also negatively regulates autophagosome formation and autophagy flux.To investigate the mechanism by which USP45 activates autophagy, we examined the effects of USP45 depletion on the expression of key autophagy-regulating proteins."
40067150
USP45 affects alpha-amylase
| 1
USP45 activates alpha-amylase. 1 / 1
| 1
reach
"Fusion of the usp45 promoter region and various parts of the leader sequence to an amyS gene devoid of its signal sequence, showed that in Escherichia coli the first 19, 20, and 27 residues of the Usp45 leader are able to direct alpha-amylase secretion."
8413193
USP45 affects acmA
| 1
USP45 activates acmA. 1 / 1
| 1
reach
"To verify whether IL-23-specific ILP protein binders can be produced in L. lactis host cells, DNA sequences coding for ILP proteins (ILP030, ILP317 and ILP323 variants) were genetically fused to Usp45 secretion signal and C-terminal domain of AcmA protein (cA) containing LysM repeats (LysM) for peptidoglycan anchoring."
29966384
USP45 affects YLPM1
1 |
USP45 binds YLPM1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects WIZ
1 |
WIZ binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45 affects V-ATPases
| 1
USP45 activates V-ATPases. 1 / 1
| 1
reach
"dUsp45/USP45 impairs lysosomal acidification and disrupts the localization of V-ATPases to lysosomes by modulating actin structures."
40067150
USP45 affects USP1
| 1
FBXL7 binds USP1 and USP45. 1 / 1
| 1
sparser
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12. xref These deubiquitylation enzymes could counteract SCF Fbxl18 to remove ubiquitin from Fbxl7 to prevent its degradation."
25654763
USP45 affects ULK1
| 1
USP45 decreases the amount of ULK1. 1 / 1
| 1
reach
"Additionally, USP45 depletion led to increased expression of ULK1, VPS34, and Beclin1 (Fig. 3, J and K), which are key regulators of autophagy initiation and autophagosome formation."
40067150
USP45 affects UBC
1 |
UBC binds USP45. 1 / 1
1 |
biogrid
No evidence text available
29576527
USP45 affects TMOD3
1 |
USP45 binds TMOD3. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects TGFBR1
1 |
TGFBR1 binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
USP45 affects Spindly
| 1
USP45 activates Spindly. 1 / 1
| 1
reach
"USP45 has been demonstrated to modulate the stability of Spindly and MYC proteins through deubiquitination (Conte et al., 2018; Tu et al., 2023)."
40067150
USP45 affects STX3
| 1
USP45 increases the amount of STX3. 1 / 1
| 1
reach
"In addition, USP45 overexpression up-regulated protein levels of NCAPD3, NCOA4, STX3, and KDELR2, while USP45 knockdown down-regulated them."
38868575
USP45 affects SRBD1
1 |
USP45 binds SRBD1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects SP
| 1
SP binds USP45. 1 / 1
| 1
sparser
"The halo formed by SP Usp45 –LysL was weaker than that of native LysL."
39153967
USP45 affects SMURF1
1 |
SMURF1 binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
USP45 affects SMAD1
1 |
SMAD1 binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
USP45 affects SF3A2
1 |
USP45 binds SF3A2. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects SAFB
1 |
USP45 binds SAFB. 1 / 1
1 |
biogrid
No evidence text available
19615732
reach
"Later, other groups showed that mutations in USP45 in human patients cause Leber congenital amaurosis, a severe form of retinal degeneration [28], overall supporting the validity of this type of analysis to unveil plausible candidates for retinal dystrophies."
31819342
USP45 affects RTCA
1 |
USP45 binds RTCA. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects RBM7
1 |
RBM7 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45 affects RALY
1 |
USP45 binds RALY. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects Prkaa1
1 |
Prkaa1 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45 affects PTPRC
| 1
USP45 binds PTPRC. 1 / 1
| 1
sparser
"Our study implicates that biallelic mutations in USP45 are associated with the occurrence of LCA."
30573563
USP45 affects PPP5C
1 |
PPP5C binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45 affects POLR2G
1 |
USP45 binds POLR2G. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects PIK3CG
1 |
USP45 binds PIK3CG. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects PIK3C3
| 1
USP45 decreases the amount of PIK3C3. 1 / 1
| 1
reach
"Additionally, USP45 depletion led to increased expression of ULK1, VPS34, and Beclin1 (Fig. 3, J and K), which are key regulators of autophagy initiation and autophagosome formation."
40067150
USP45 activates Neoplasm Metastasis. 1 / 1
| 1
reach
"In the present study, we demonstrated that the expression level of USP45 in the majority types of tumors was positively correlated with the tumor stemness index, suggesting that upregulation of USP45 may promote the proliferation and metastasis of tumor cells."
35836933
USP45 affects Ncbp2
1 |
Ncbp2 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45 affects NOP53
1 |
USP45 binds NOP53. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects NCAPD3
| 1
USP45 increases the amount of NCAPD3. 1 / 1
| 1
reach
"In addition, USP45 overexpression up-regulated protein levels of NCAPD3, NCOA4, STX3, and KDELR2, while USP45 knockdown down-regulated them."
38868575
USP45 affects MYL6
1 |
USP45 binds MYL6. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects MTOR
| 1
USP45 activates MTOR. 1 / 1
| 1
reach
"While mTORC1 protein expression remained unaffected, the phosphorylation of its downstream target, p70 ribosomal protein S6 kinase (S6K), was reduced in USP45-depleted cells, indicating a decrease in mTOR activity (Fig. 3, H and I)."
40067150
USP45 affects LiaRS
| 1
USP45 activates LiaRS. 1 / 1
| 1
reach
"Usp45 and TEM-1 IA (2)-lactamase specifically induce the LiaRS dependent response."
22624725
USP45 activates Leber Congenital Amaurosis. 1 / 1
| 1
reach
"Later, other groups showed that mutations in USP45 in human patients cause Leber congenital amaurosis, a severe form of retinal degeneration [28], overall supporting the validity of this type of analysis to unveil plausible candidates for retinal dystrophies."
31819342
USP45 affects LCN2
| 1
USP45 activates LCN2. 1 / 1
| 1
reach
"Construction of Lcn2 under the P1 promoter allows for constitutive expression, whereas USP45 encodes for the sequence signal to drive the secretion of Lcn2 from L. lactis."
28445245
USP45 affects Kif3c
1 |
Kif3c binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
USP45 affects KDELR2
| 1
USP45 increases the amount of KDELR2. 1 / 1
| 1
reach
"In addition, USP45 overexpression up-regulated protein levels of NCAPD3, NCOA4, STX3, and KDELR2, while USP45 knockdown down-regulated them."
38868575
USP45 affects K48
| 1
USP45 binds MYC and K48. 1 / 1
| 1
reach
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
USP45 affects IGKV4-1
1 |
USP45 binds IGKV4-1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects H2BC9
1 |
USP45 binds H2BC9. 1 / 1
1 |
biogrid
No evidence text available
30021884
USP45 affects H2BC18
1 |
H2BC18 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
30021884
USP45 affects FlaG
| 1
USP45 binds FlaG and SPDL1. 1 / 1
| 1
sparser
"This suggests that Spindly could be potentially be mono-ubiquitylated instead of poly-ubiquitylated: Treatment of the IP of Spindly-FLAG with recombinant USP45 WT showed a removal of the potential Spindly mono-ubiquitylation band (Fig.  xref , right panel)."
30258100
USP45 affects FLNA
1 |
USP45 binds FLNA. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects FARS2
1 |
USP45 binds FARS2. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects DNA repair
| 1
USP45 inhibits DNA repair. 1 / 1
| 1
reach
"It was shown that USP45 could inhibit the ubiquitin-proteasome degradation pathway of ERCC1 protein, a DNA repair-related nucleic acid endonuclease, thereby enhancing the ability of tumor cells to repair DNA damage.4 KEGG pathway analysis showed that USP45 may be associated with the MAPK signaling pathway, AMPK signaling pathway, Hippo signaling pathway, cell cycle, p53 signaling pathway, and TGF-β signaling pathway (Fig. 1N and Table S5)."
38868575
USP45 affects DNA Damage
| 1
USP45 activates DNA Damage. 1 / 1
| 1
reach
"Taken together, these data indicate that USP45 localises at sites of UV induced DNA damage, and at intra- and interstrand cross-links, and that USP45 is required, like ERCC1 for efficient NER."
25538220
USP45 affects CSNK2B
1 |
CSNK2B binds USP45. 1 / 1
1 |
biogrid
No evidence text available
35271311
USP45 affects CORO1C
1 |
USP45 binds CORO1C. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects CFL1
1 |
CFL1 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
30021884
USP45 affects CDK1
1 |
USP45 binds CDK1. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 affects BMPR1B
1 |
BMPR1B binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
USP45 affects BECN1
| 1
USP45 decreases the amount of BECN1. 1 / 1
| 1
reach
"Additionally, USP45 depletion led to increased expression of ULK1, VPS34, and Beclin1 (Fig. 3, J and K), which are key regulators of autophagy initiation and autophagosome formation."
40067150
USP45 affects ATP6V0D
| 1
USP45 activates ATP6V0D. 1 / 1
| 1
reach
"Interestingly, the ablation of USP45 significantly enhanced the localization of the V-ATPase V0 subunit d (ATP6V0D) at late endosomes (Rab 7) and lysosomes (LAMP2), while having no effects on its localization at early endosomes (EEA1) (Fig. 4, E and F)."
40067150
USP45 affects ARID1A
| 1
USP45 binds ARID1A. 1 / 1
| 1
reach
"We further investigated shared segments and WES for evidence supporting the complexes USP45 and ARID1A are involved in."
29389935
USP45 affects APOH
| 1
USP45 increases the amount of APOH. 1 / 1
| 1
reach
"Directly using the broth supernatant while omitting the purification process is economical.In conclusion, the solubilized form of BG was expressed in L. lactis NZ3900; the addition of Usp45 caused ext[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31112758
USP45 affects AKR1B15
1 |
AKR1B15 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
39358380
USP45 affects ABL1
| 1
USP45 increases the amount of ABL1. 1 / 1
| 1
reach
"It revealed that UCHL3, USP7, USP8 and USP45 increased c‐Abl protein level (Figure 2A)."
38082439
USP1 affects USP45
| 1
FBXL7 binds USP1 and USP45. 1 / 1
| 1
sparser
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12. xref These deubiquitylation enzymes could counteract SCF Fbxl18 to remove ubiquitin from Fbxl7 to prevent its degradation."
25654763
UBC affects USP45
1 |
UBC binds USP45. 1 / 1
1 |
biogrid
No evidence text available
29576527
TMOD3 affects USP45
1 |
USP45 binds TMOD3. 1 / 1
1 |
biogrid
No evidence text available
19615732
TGFBR1 affects USP45
1 |
TGFBR1 binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
T-2 toxin affects USP45
1 |
T-2 toxin increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
36695871
Smoke affects USP45
1 |
Smoke increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
38245732
SRBD1 affects USP45
1 |
USP45 binds SRBD1. 1 / 1
1 |
biogrid
No evidence text available
19615732
SPDL1 affects FlaG
| 1
USP45 binds FlaG and SPDL1. 1 / 1
| 1
sparser
"This suggests that Spindly could be potentially be mono-ubiquitylated instead of poly-ubiquitylated: Treatment of the IP of Spindly-FLAG with recombinant USP45 WT showed a removal of the potential Spindly mono-ubiquitylation band (Fig.  xref , right panel)."
30258100
SP affects USP45
| 1
SP binds USP45. 1 / 1
| 1
sparser
"The halo formed by SP Usp45 –LysL was weaker than that of native LysL."
39153967
SMURF1 affects USP45
1 |
SMURF1 binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
SMAD1 affects USP45
1 |
SMAD1 binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
SF3A2 affects USP45
1 |
USP45 binds SF3A2. 1 / 1
1 |
biogrid
No evidence text available
19615732
SAFB affects USP45
1 |
USP45 binds SAFB. 1 / 1
1 |
biogrid
No evidence text available
19615732
RTCA affects USP45
1 |
USP45 binds RTCA. 1 / 1
1 |
biogrid
No evidence text available
19615732
RBM7 affects USP45
1 |
RBM7 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
RALY affects USP45
1 |
USP45 binds RALY. 1 / 1
1 |
biogrid
No evidence text available
19615732
Prkaa1 affects USP45
1 |
Prkaa1 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
PTPRC affects USP45
| 1
USP45 binds PTPRC. 1 / 1
| 1
sparser
"Our study implicates that biallelic mutations in USP45 are associated with the occurrence of LCA."
30573563
PPP5C affects USP45
1 |
PPP5C binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
POLR2G affects USP45
1 |
USP45 binds POLR2G. 1 / 1
1 |
biogrid
No evidence text available
19615732
PIK3CG affects USP45
1 |
USP45 binds PIK3CG. 1 / 1
1 |
biogrid
No evidence text available
19615732
USP45 is modified
| 1
USP45 is ubiquitinated. 1 / 1
| 1
sparser
"Given that USP45 is a DUB, we decided to explore the ubiquitylation in the Spindly-USP45 complex."
30258100
Ncbp2 affects USP45
1 |
Ncbp2 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
NOP53 affects USP45
1 |
USP45 binds NOP53. 1 / 1
1 |
biogrid
No evidence text available
19615732
MYL6 affects USP45
1 |
USP45 binds MYL6. 1 / 1
1 |
biogrid
No evidence text available
19615732
MYC affects K48
| 1
USP45 binds MYC and K48. 1 / 1
| 1
reach
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
MIR665 affects USP45
1 |
MIR665 decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
27292025
MIR630 affects USP45
1 |
MIR630 decreases the amount of USP45. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
20371350
Leber Congenital Amaurosis activates USP45. 1 / 1
| 1
reach
"Biallelic LoF mutations in USP45 cause Leber congenital amaurosis, a severe form of inherited retinal dystrophies that results in irreversible childhood blindness (45)."
35662507
Lane 1 affects USP45
| 1
Lane 1 activates USP45. 1 / 1
| 1
reach
"Lane 1 : DNA molecular weight marker, Lane 2 : PCR products of usp45.1 2Figure 2."
22998828
L-methionine affects USP45
1 |
L-methionine decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
20938992
Kif3c affects USP45
1 |
Kif3c binds USP45. 1 / 1
1 |
biogrid
No evidence text available
26496610
K48 affects USP45
| 1
USP45 binds MYC and K48. 1 / 1
| 1
reach
"Co-IP assays confirmed that USP45 binds directly to MYC, selectively removing K48-linked ubiquitin chains from MYC, thereby intensifying Dox resistance in cancer cells."
38702734
IGKV4-1 affects USP45
1 |
USP45 binds IGKV4-1. 1 / 1
1 |
biogrid
No evidence text available
19615732
H2BC9 affects USP45
1 |
USP45 binds H2BC9. 1 / 1
1 |
biogrid
No evidence text available
30021884
H2BC18 affects USP45
1 |
H2BC18 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
30021884
FlaG affects USP45
| 1
USP45 binds FlaG and SPDL1. 1 / 1
| 1
sparser
"This suggests that Spindly could be potentially be mono-ubiquitylated instead of poly-ubiquitylated: Treatment of the IP of Spindly-FLAG with recombinant USP45 WT showed a removal of the potential Spindly mono-ubiquitylation band (Fig.  xref , right panel)."
30258100
F_actin affects USP45
| 1
USP45 binds F_actin. 1 / 1
| 1
sparser
"USP45 depletion–induced F-actin patch formation is crucial for V-ATPase lysosome localization and lysosomal acidification."
40067150
FR900359 affects USP45
1 |
FR900359 dephosphorylates USP45. 1 / 1
1 |
ctd
No evidence text available
37730182
FLNA affects USP45
1 |
USP45 binds FLNA. 1 / 1
1 |
biogrid
No evidence text available
19615732
FBXL7 affects USP1, and USP45
| 1
FBXL7 binds USP1 and USP45. 1 / 1
| 1
sparser
"For example, Fbxl7 directly associates to USP1 and USP45, and indirectly is linked to USP12. xref These deubiquitylation enzymes could counteract SCF Fbxl18 to remove ubiquitin from Fbxl7 to prevent its degradation."
25654763
FARS2 affects USP45
1 |
USP45 binds FARS2. 1 / 1
1 |
biogrid
No evidence text available
19615732
Cuprizon affects USP45
1 |
Cuprizon decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
26577399
CSNK2B affects USP45
1 |
CSNK2B binds USP45. 1 / 1
1 |
biogrid
No evidence text available
35271311
CORO1C affects USP45
1 |
USP45 binds CORO1C. 1 / 1
1 |
biogrid
No evidence text available
19615732
CFL1 affects USP45
1 |
CFL1 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
30021884
CDK1 affects USP45
1 |
USP45 binds CDK1. 1 / 1
1 |
biogrid
No evidence text available
19615732
BMPR1B affects USP45
1 |
BMPR1B binds USP45. 1 / 1
1 |
hprd
No evidence text available
15761153
Aflatoxin B2 alpha methylates USP45. 1 / 1
1 |
ctd
No evidence text available
30157460
Actin affects USP45
| 1
USP45 binds Actin. 1 / 1
| 1
reach
"Firstly, USP45 interacts with and deubiquitinates the actin-associated protein Coro1B, thereby stabilizing its protein levels."
40067150
ARID1A affects USP45
| 1
USP45 binds ARID1A. 1 / 1
| 1
reach
"We further investigated shared segments and WES for evidence supporting the complexes USP45 and ARID1A are involved in."
29389935
AQP1 affects USP45
| 1
AQP1 activates USP45. 1 / 1
| 1
reach
"The siRNA targeting MYH10 (siMYH10), MYH9 (siMYH9), and USP45 (siUSP45) were all synthesized by Guangzhou Ribobio Co., Ltd. (Guangzhou, China)."
36929633
AKR1B15 affects USP45
1 |
AKR1B15 binds USP45. 1 / 1
1 |
biogrid
No evidence text available
39358380
3-isobutyl-1-methyl-7H-xanthine decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
35504463
3,4-methylenedioxymethamphetamine decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
30071829
2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
31545209
17beta-estradiol affects USP45
1 |
17beta-estradiol increases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
31614463
1,2-dimethylhydrazine decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
22206623
(R)-pantothenic acid decreases the amount of USP45. 1 / 1
1 |
ctd
No evidence text available
35504463