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CHEK2 phosphorylates TP53. 10 / 348
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rlimsp
"CHK2 is known to phosphorylate and stabilize p53."

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"However, p53 is required to release Histone H1.2 from the nucleus upon damage induced by ionizing radiation in a process requiring Chk2 phosphorylation and stabilization of p53."

sparser
"While Chk2 activates multiple downstream targets in addition to p53, and the p53 protein may be activated through multiple post-transcriptional events [ xref ], the finding that CHEK2 mutations may substitute for TP53 mutations as a cause of chemo-resistance indicates Chk2 phosphorylation of the p53 protein to play a pivotal role executing cell death in response to anthracycline therapy in breast cancer."

sparser
"Additionally, CHK2 phosphorylates the N-terminal activation domain of P53 to regulate the response of P53 to DNA damage."

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"Activated Chk1 and Chk2 phosphorylate p53 at multiple positions, with Chk1 phosphorylating multiple sites within the intrinsically disordered C-terminus of p53 113 and Chk2 primarily targeting the intrinsically disordered N-terminal region of p53 114."

sparser
"CHEK2 can promote the phosphorylation of tumor suppressor gene p53 (Ser20), block the binding of murine double micro-2 (MDM2) protein to p53 and its role in degradation of p53, thus improving the stability of p53 in cells [ xref ]."

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"In this regard, we reported that hMSH2, a mismatch repair protein, accumulates to DNA damage site during cisplatin treatment and further recruits ATR and ATRIP complex to activate Chk2, which then phosphorylate p53 to induce further apoptosis."

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"In the present study, it was also demonstrated that valproic acid activates CHK2, a kinase that phosphorylates p53 subsequent to DNA damage, in the MCF7 cells and primary MEFs."

sparser
"The phosphorylation of CHK2 and p53 was also increased by EEOM, indicating that activated CHK2 triggers p53 phosphorylation to increase the stability of p53. xref , xref However, EEOM did not show any big difference in the protein levels of Cdc25C. These results suggest that EEOM-mediated G2/M arrest occurs by inhibition of CDK/cyclin complex via p53-dependent p21 induction."

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"Studies of p53 posttranslational stabilization reveal that DNA dependent protein kinases, ATM and CHK2, are activated by gamma radiation and phosphorylate p53 at N-terminal sites near the region for M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
CHEK2 phosphorylates TP53 on S20. 10 / 109
1 1 1 | 42 52 9

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"20 ATM and ATR directly phosphorylates p53 at Ser15, whereas CHK-2 and CHK-1 phosphorylates Ser20 on p53."

sparser
"P53 is phosphorylated at S20 by PLK3 and Chk2 following ionizing radiation xref , xref ."

sparser
"Several studies showed that DNA double-strand breaks induce phosphorylation of p53 S15 by ATM or DNA-PK. xref , xref ATM also activates Chk2, which in turn phosphorylates p53 on S20 which is part of the MDM2 binding site. xref , xref These findings suggested a model that p53 phosphorylation on the N terminus disrupts MDM2 binding and results in p53 stabilization."

rlimsp
"Chk2 directly phosphorylated p53 on serine 20, which is known to interfere with Mdm2 binding."

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"Chk2 phosphorylates p53 at Ser 20, inhibiting MDM2-p53 protein binding [XREF_BIBR] but also at several sites located in the C-terminal domain of the p53 protein [XREF_BIBR]."

sparser
"In addition, following DNA damage, Chk2 and p53 are enriched in PML NBs, where PML promotes Chk2 autophosphorylation, phosphorylation of p53 by Chk2 at Ser20 and subsequent stabilization of p53 ( xref ; xref )."

rlimsp
"Activated Chk2 subsequently phosphorylated p53 at Ser 20 [39], which upregulated the expression of DNA-damage and cell cycle arrest-related genes (p21 and p27) [40]."

"The tumour suppressor protein p53 is stabilised and activated in response to ionising radiation. This is known to depend on the kinase atm;recent results suggest atm acts via the downstream kinase chk2/hcds1, which stabilises p53 at least in part by direct phosphorylation of residue serine 20"

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"It is known that DNA damage response (DDR) induced by double strand breaks or uncapped telomeres can result in activation of ATM and Chk2; phosphorylation of p53 by Chk1 or Chk2 at Ser 20 stabilizes p53 by dissociating it from HDM2 and Mdm2 XREF_BIBR, XREF_BIBR."

sparser
"The serine 20 of p53 is phosphorylated by Chk2, and thereby interrupts the binding of p53 to Mdm2 and interrupts p53 ubiquitination, resulting in greater stability of p53 [ xref ]."
CHEK2 phosphorylates TP53 on S15. 10 / 23
1 4 | 12 6

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"The DNA damage response manifested itself by H2AX phosphorylation, ATM and Chk2 activation and phosphorylation of p53 on Ser15."

sparser
"For instance, the DNA damage insult mediates phosphorylation of p53 at Ser15 and Ser20 by ATM, ATR, DNA-PK, Chk1, and Chk2, resulting in an increase of transcriptional activity of p53 [ xref , xref , xref ]."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability. We have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

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"After DNA damage, ATM and CHK2 phosphorylate p53 (S15 and S20), thus reducing its ability to bind MDM2 and contributing to its stabilization [XREF_BIBR, XREF_BIBR]."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

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"Phosphorylation of p53 at Ser 15 by ATM and at Ser 20 by Chk2 inhibits p53 degradation by MDM2, a RING finger E3 ligase which ubiquitinates and degrades p53 through ubiquitin mediated proteolysis."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

sparser
"In contrast to cdc25a, p53 is phosphorylated by Chk2 at sites Ser 15 and 20, stabilizing p53 expression and leading to enhanced transcriptional activity [ xref ]."

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"Importantly, Chel A induced p53 protein expression was blocked in Chk2-/- cells, and the phosphorylation of p53 at Ser20 and Ser15 were almost abrogated by Chk2 knockout."

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"Whereas CHK1 inbibits the CDC25 phosphatase, inducing phosphorylation and inactivation of G2 CDKs, ATM and CHK2 phosphorylate p53 at Ser 15 in the N-terminal transcriptional activation domain."
CHEK2 phosphorylates TP53 on serine. 10 / 22
| 21 1

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"In addition, the ATM dependent chk2 protein phosphorylates p53 at serine 20."

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"Note that ATM also activates check point kinase 2 (Chk2), which then phosphorylates p53 on Serine 20 [27]."

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"Recent data [45-47] show that activated Chk2 and Chk1 phosphorylate p53 on serine 20, an event which leads to decreased protein turnover and thus accumulation of p53."

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"ATM is also known to phosphorylate Chk2, which subsequently phosphorylates p53 on serine 20 [XREF_BIBR]."

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"On the other hand, activation of ATM leads to direct phosphorylation of p53 on serine 15 and 37, as well as phosphorylation and activation of another checkpoint kinase (CHK2), which also phosphorylates p53 on serine 20."

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"PML neutralizes the inhibitory effects of Mdm2 by prolonging the stress induced phosphorylation of p53 on serine 20, a site of the checkpoint kinase 2 (Chk2)."

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"For example, after DNA damage p53 is multiply phosphorylated, with the phosphorylation of Serine 15 by ATM enhancing its transcriptional activity and promoting other phosphorylations, while the ATM ac[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"XREF_BIBR In response to ionizing radiation, the kinase activity of ATM is enhanced, leading to phosphorylation of p53 on serine 15 and activation of the checkpoint kinase Chk2, which, in turn, phosphorylates p53 on serine 20."

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"The serine 20 of p53 is phosphorylated by Chk2, and thereby interrupts the binding of p53 to Mdm2 and interrupts p53 ubiquitination, resulting in greater stability of p53 [XREF_BIBR]."

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"P53 is targeted for deg- radation by binding with the MDM2 protein, which transfers a small polypeptide molecule called ubiquitin onto p53.37-4In response to IR, ATM phosphorylates MDM2 on serine 395 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
CHEK2 phosphorylates TP53 at position 20. 10 / 18
| 18

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"Note that ATM also activates check point kinase 2 (Chk2), which then phosphorylates p53 on Serine 20 [27]."

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"XREF_BIBR In response to ionizing radiation, the kinase activity of ATM is enhanced, leading to phosphorylation of p53 on serine 15 and activation of the checkpoint kinase Chk2, which, in turn, phosphorylates p53 on serine 20."

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"This group has demonstrated that Chk2 is able to phosphorylate p53 on serine 20, and that this phosphorylation event triggers dissociation of preformed complexes between p53 and MDM2 under in vitro co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Checkpoint kinase 2 (CHK2) a key downstream target of ATM (Matsuoka et al., 1998) and mediator of ATM signalling also phosphorylates p53, on serine 20 (Chehab et al., 1999, 2000)."

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"ATM phosphorylates p53 at serine 15 XREF_BIBR, XREF_BIBR, whereas Chk2 phosphorylates p53 at serine 20 in response to activation by ATM XREF_BIBR, XREF_BIBR."

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"ATM-activated Chk2 kinase also phosphorylates p53 at the threonine-18 and serine-20 residues [63,64]."

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"P53 is targeted for deg- radation by binding with the MDM2 protein, which transfers a small polypeptide molecule called ubiquitin onto p53.37-4In response to IR, ATM phosphorylates MDM2 on serine 395 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"ATM is also known to phosphorylate Chk2, which subsequently phosphorylates p53 on serine 20 [XREF_BIBR]."

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"Moreover, PML is capable of enhancing p53 stability through sequestering MDM2 [XREF_BIBR] or inhibiting MDM2 mediated p53 degradation via prolonging the phosphorylation of p53 on serine 20 by Chk2 [XREF_BIBR]."

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"XREF_BIBR, XREF_BIBR Chk2 directly phosphorylates p53 on serine 20, which is known to interfere with Mdm2 binding, thus providing a mechanism for the increased stability of p53 in response to DNA damage."
CHEK2 phosphorylates TP53 on T18. 10 / 11
1 4 | 3 3

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"In contrast to Cdc25A, p53 is phosphorylated not only by Chk1 and Chk2 (on Thr18, Ser20, and possibly other residues) but also directly by the upstream checkpoint kinases ATM and ATR, particularly, on[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

sparser
"ATM also activates CHK2, a serine threonine kinase, which phosphorylates p53 at threonine 18 and serine 20."

No evidence text available

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"The N-terminal activation domain of p53 does not contain the classical Chk1 and Chk2 consensus target sequence found in other substrates such as the Cdc25 phosphatases, and the physiological tetrameri[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"CHK2 also phosphorylates P53 Thr18 and Ser20 to promote p21 accumulation and to maintain G2/M arrest."

sparser
"ATM-activated Chk2 kinase also phosphorylates p53 at the threonine-18 and serine-20 residues [ xref , xref ]."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."
CHEK2 phosphorylates TP53 on S378. 8 / 8
1 3 | 4

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

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"The Chk1 and Chk2 kinases phosphorylate p53 at S366, S378 and T387."

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"Chk2 is known to mediate phosphorylation of p53 at Ser 366 and Ser 378 in response to genotoxic stresses XREF_BIBR."

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"However, during the later stages, CHK2 may contribute to p53 phosphorylation at Ser366 and Ser378, resulting in p53 degradation through the ubiquitin-proteasome pathway and blocking of p53 mediated apoptosis [XREF_BIBR] and the EBV protein kinase BGLF4 contributes to p27 phosphorylation and its ubiquitin-proteasome dependent degradation [XREF_BIBR]."

No evidence text available

"The cell cycle checkpoint kinases CHK1 and CHK2 have been shown to phosphorylate multiple sites in the N-terminal domain of p53, consequently leading to p53 stabilization and activation. Phosphorylation of at least three of these sites, Ser366, Ser378, and Thr387, was induced by DNA damage."

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"ATM dependent dephosphorylation of p53 at S376 and Chk1 and Chk2 dependent phosphorylation of p53 at S378, S366 and T387 generate 14-3-3 binding sites in vivo."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."
CHEK2 phosphorylates TP53 on S366. 7 / 7
1 2 | 4

"The cell cycle checkpoint kinases CHK1 and CHK2 have been shown to phosphorylate multiple sites in the N-terminal domain of p53, consequently leading to p53 stabilization and activation. Phosphorylation of at least three of these sites, Ser366, Ser378, and Thr387, was induced by DNA damage."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

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"ATM dependent dephosphorylation of p53 at S376 and Chk1 and Chk2 dependent phosphorylation of p53 at S378, S366 and T387 generate 14-3-3 binding sites in vivo."

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"Chk2 is known to mediate phosphorylation of p53 at Ser 366 and Ser 378 in response to genotoxic stresses XREF_BIBR."

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"However, during the later stages, CHK2 may contribute to p53 phosphorylation at Ser366 and Ser378, resulting in p53 degradation through the ubiquitin-proteasome pathway and blocking of p53 mediated apoptosis [XREF_BIBR] and the EBV protein kinase BGLF4 contributes to p27 phosphorylation and its ubiquitin-proteasome dependent degradation [XREF_BIBR]."

No evidence text available

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"The Chk1 and Chk2 kinases phosphorylate p53 at S366, S378 and T387."
CHEK2 phosphorylates TP53 on S37. 5 / 5
1 4 |

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."

"The cell cycle checkpoint kinases CHK1 and CHK2 have been shown to phosphorylate multiple sites in the N-terminal domain of p53, consequently leading to p53 stabilization and activation. Phosphorylation of at least three of these sites, Ser366, Ser378, and Thr387, was induced by DNA damage. On activation, both of these kinases also phosphorylate multiple sites in the p53 N-terminal domain. These include Ser15, Thr18, Ser20, and Ser37, which are all DNA-damageinducible sites"

No evidence text available

"Chk1/chk2 and atm/atr also phosphorylate the effector p53, increasing its stability.We Have demonstrated that the human homologs of the checkpoint kinases, chk1 and chk2/hcds1, phosphorylate at least three dna damage-inducible phosphorylation sites in p53."
Kinase-active CHEK2 phosphorylates TP53 on S20. 4 / 4
2 2 |

"12111733;10801407;15489221;15254178"

"The CHK2 phosphorylates p53 at S20 and in turn activates it."

"Two peptides derived from the DNA-binding domain of p53 bind to CHK2 and stimulate phosphorylation of full-length p53 at Thr 18 and Ser 20, thus identifying CHK2-docking sites."

"ATM is one of the major kinases that phosphorylate p53 at Ser15 after IR and that ATM also phosphorylates and activates Chk2 after IR, which then phosphorylates p53 at Ser20"
CHEK2 phosphorylates TP53 on T387. 3 / 3
| 3

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"ATM dependent dephosphorylation of p53 at S376 and Chk1 and Chk2 dependent phosphorylation of p53 at S378, S366 and T387 generate 14-3-3 binding sites in vivo."

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"The Chk1 and Chk2 kinases phosphorylate p53 at S366, S378 and T387."

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"Chk1 and Chk2 can also phosphorylate additional p53 residues including T387 by Chk1, T18 and S366 by Chk2, and 4 residues (S313, S314, T377, S378) in the C-terminus by both kinases [XREF_BIBR]."
Phosphorylated CHEK2 phosphorylates TP53. 3 / 3
| 3

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"The phosphorylated CHK2 then phosphorylates p53 and CDC25."

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"Furthermore, phosphorylation of checkpoint kinases CHK1 and CHK2 promotes the phosphorylation of p53 and enhances the stability of p53, hence causing activation of CASP3."

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"The phosphorylated CHK2 then phosphorylates p53 and CDC25."
CHEK2 phosphorylates TP53 on threonine. 2 / 2
| 2

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"ATM-activated Chk2 kinase also phosphorylates p53 at the threonine-18 and serine-20 residues [63,64]."

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"ATM also activates CHK2, a serine threonine kinase, which phosphorylates p53 at threonine 18 and serine 20."
CHEK2 phosphorylates TP53 on S313. 1 / 1
| 1

rlimsp
"While Chk2 phosphorylates p53 at Ser 20, thereby stabilizing p53 by preventing MDM2 binding [19], Chk2 also phosphorylates p53 at six additional sites, including Ser 313 and Ser 314 located in the nuclear localization signal domain of p53 [51]."
CHEK2 phosphorylated on S379, T383, T387, and T68 phosphorylates TP53 phosphorylated on S15 on S20. 1 / 1
1 |

No evidence text available
Modified CHEK2 leads to the phosphorylation of TP53 on S20. 1 / 1
| 1

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"In vivo, ectopic expression of wild-type Chk2 and hCds1 led to increased p53 stabilization after DNA damage, whereas expression of a dominant negative Chk2 and hCds1 mutant abrogated both phosphorylation of p53 on Ser 20 and p53 stabilization."
CHEK2 phosphorylates TP53 on T377. 1 / 1
| 1

sparser
"In fact, various kinases such as TFIIH CDK7-cycH-p36 [ xref ], protein kinase C [ xref , xref ], CHK1, and CHK2 [ xref ] reportedly phosphorylate at least p53 Thr377."
Modified CHEK2-K249Q leads to the phosphorylation of TP53. 1 / 1
| 1

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"Overexpression of K235Q and K249Q CHK2 further elicited the phosphorylation of p53 but K235R and K249R CHK2, a potential inactive protein, did not."
Kinase-active CHEK2 leads to the phosphorylation of TP53 on S15. 1 / 1
1 |

"The respective kinetics of p53-Ser15 phosphorylation versus ATM and Chk2 activation suggest that in response to DNA damage by TPT or MXT, Chk2 rather than ATM mediates p53 phosphorylation."
Phosphorylated CHEK2 leads to the phosphorylation of TP53 on S15. 1 / 1
| 1

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"Correlatively, Chk2 phosphorylation was increased, which was accompanied with increases in both total and phosphorylated p53 (ser 15) (XREF_FIG)."
Modified CHEK2 leads to the phosphorylation of TP53. 1 / 1
| 1

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"Increased CHK2 expression stimulated CHK2 activation and increased the phosphorylation of p53, the target of CHK2."
Modified CHEK2-K235Q leads to the phosphorylation of TP53. 1 / 1
| 1

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"Overexpression of K235Q and K249Q CHK2 further elicited the phosphorylation of p53 but K235R and K249R CHK2, a potential inactive protein, did not."
Kinase-active CHEK2 phosphorylates TP53 on T18. 1 / 1
1 |

"Two peptides derived from the DNA-binding domain of p53 bind to CHK2 and stimulate phosphorylation of full-length p53 at Thr 18 and Ser 20, thus identifying CHK2-docking sites."
CHEK2 phosphorylates TP53 on S367. 1 / 1
| 1

sparser
"HDMX is a negative regulator of p53 that is phosphorylated by Chk2 on Ser342 and Ser367 upon exposure to IR, leading to its degradation."
CHEK2 phosphorylates TP53 at position 18. 1 / 1
| 1

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"ATM-activated Chk2 kinase also phosphorylates p53 at the threonine-18 and serine-20 residues [63,64]."
CHEK2 phosphorylates TP53 phosphorylated on S15 on S20. 1 / 1
1 |

No evidence text available
CHEK2 leads to the phosphorylation of TP53 at position 15. 1 / 1
| 1

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"ATM and its effector protein Chk2 play a role in the phosphorylation of p53 (serine 15) and activation of p53 in response to oxidative stress [XREF_BIBR] and also in cell cycle arrest in response to oxidative stress [XREF_BIBR, XREF_BIBR]."