IndraLab

Statements


UCHL1 affects NOX4
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UCHL1 deubiquitinates NOX4. 10 / 12
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"Immunoprecipitation studies confirmed that UCH-L1 deubiquitinates NOX4, suggesting that UCH-L1 might be involved in H 2 O 2 -mediated cell invasion by regulating the NOX4 activity through deubiquitination."

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"On the other hand, NOX4 overexpressed in HeLa cells happens to be ubiquitinated, and NOX4 following deubiquitination by UCH-L1, restored H2O2 generating activity."

"These findings indicate that H2O2 levels regulated by UCH-L1 are necessary for cell invasion to occur and demonstrate that UCH-L1 promotes cell invasion by up-regulating H2O2 via deubiquitination of NOX4."

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"In the present study, we have shown that UCH-L1 deubiquitinates NOX4, thereby up-regulating its ability to generate H 2 O 2 and promoting the invasive potential of B16F10 cells both in vitro and in vivo."

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"UCH-L1 restores H 2 O 2 -generating activity of NOX4 by deubiquitinating NOX4."

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"We further investigated whether endogenous NOX4 is also deubiquitinated by UCH-L1 in B16F10 cells."

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"We further showed that UCH-L1 increases ROS levels in HUVEC by deubiquitinating NOX4 in VEGF signaling."

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"These immunoprecipitation studies confirm that UCH-L1 deubiquitinates NOX4 in HUVECs, and suggest that UCH-L1 is involved in ROS mediated angiogenesis in HUVECs by modulating NOX4 activity via deubiqu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Fig. 4 B shows that ubiquitination of NOX4 was markedly decreased by adding back of control UCH-L1 in UCH-L1-knocked down HUVECs."

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"Also, we demonstrated that UCH-L1 restores H 2 O 2 -gernerating activity of NOX4 by deubiquitinating NOX4."
UCHL1 affects TP53
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UCHL1 deubiquitinates TP53. 6 / 6
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"However, while exploring the relationship between UCH-L1 and p53 ubiquitination, it is important to keep in mind that, despite hypotheses to the contrary [XREF_BIBR, XREF_BIBR], it is unlikely that UCH-L1 directly deubiquitinates or ubiquitinates p53 based on what is known about UCH-L1 structure and function [XREF_BIBR, XREF_BIBR]."

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"These results indicate that UCHL1 could deubiquitinate p53 and p14(ARF) and ubiquitinate MDM2 for p53 stabilization to promote p53 signaling, thus involved in nasopharyngeal carcinoma pathogenesis, whereas it is frequently silenced in this tumor."

"These results indicate that UCHL1 could deubiquitinate p53 and p14(ARF)"

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"Our previous work demonstrated that UCHL1 could activate the p14ARF-p53 signaling pathway by deubiquitinating p53 and p14ARF as well as ubiquitinating MDM2, which might be through its two opposing enzyme activities, hydrolase and ligase, further resulting in its tumor suppressive role in NPC tumorigenesis XREF_BIBR, XREF_BIBR."

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"As P53 protein is regulated through ubiquitin dependent degradation in tumorigenesis, UCHL1 promotes p53 signaling by deubiquitinating p53 and p14 ARF and ubiquitinating MDM2 for further MDM2 degradation and p53 stabilization, thus involved in NPC pathogenesis as a functional TSG XREF_BIBR."

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"These results indicate that UCHL1 could deubiquitinate p53 and p14 (ARF) and ubiquitinate MDM2 for p53 stabilization to promote p53 signaling, thus involved in nasopharyngeal carcinoma pathogenesis, whereas it is frequently silenced in this tumor."
Modified UCHL1 leads to the deubiquitination of TP53. 1 / 1
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"Similarly, Li et al. have shown that over-expression of UCH-L1 in LNCaP prostate cancer cells reduces polyubiquitination of p53, leading to inhibition of degradation of p53 by the proteasome [XREF_BIBR]."
UCHL1 affects EGFR
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UCHL1 deubiquitinates EGFR. 5 / 5
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"UCHL1 inhibits EGFR ubiquitination and degradation."

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"As noted above, Chen et al. proposed that UCHL1 deubiquitinates and stabilises EGFR, which subsequently represses ER transcription through hyperactivation of MAPK [41] and UCHL1 inhibition using LDN-57444 or knockdown induced ER expression in ER− breast cancer cells."

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"UCHL1 deubiquitinates epidermal growth factor receptor, preventing its degradation and resulting in the activation of multiple downstream signaling cascades that positively regulate cardiac hypertrophy."

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"In addition, knockdown of UCHL1 increased EGFR polyubiquitination and decreased the EGFR level with or without EGF stimulation."

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"In 2020, Chen et al. through various lines of experimental evidence provided a more detailed explanation for this correlation, proposing that UCHL1 deubiquitinates and stabilises Epidermal Growth Factor Receptor (EGFR), which in turn causes suppression of ER transcription [41]."
Modified UCHL1 leads to the deubiquitination of EGFR. 1 / 1
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"As expected, overexpression of UCHL1 (WT) significantly abrogated EGFR ubiquitination and increased EGFR levels, whereas this effect was reversed by UCHL1 (C90S; XREF_FIG)."
UCHL1 affects NTRK2
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UCHL1 deubiquitinates NTRK2. 5 / 5
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"We provide evidence that UCH-L1 can deubiquitinate TrkB directly."

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"UCH-L1 mediates the deubiquitylation of TrkB by cleaving ubiquitin (probably polyubiquitin chains) and thereby inhibits TrkB degradation."

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"Deubiquitination of TrkB by the DUB UCHL1 has been reported, which maintains expression of the receptor on the cell surface [XREF_BIBR]."

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"Together, our results suggest that the ubiquitination of TrkB is a mechanism that controls its downstream signaling pathways via the regulation of its endocytosis and postendocytic trafficking and that UCH-L1 mediates the deubiquitination of TrkB and could be a potential target for the modulation of hippocampus dependent memory."

"Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) Promotes Hippocampus-Dependent Memory via Its Deubiquitinating Effect on TrkB"
UCHL1 affects SNCA
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UCHL1 deubiquitinates SNCA. 2 / 2
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"The deubiquitination of α-synuclein aggregates by USP9X favor their degradation by autophagy, while UCH-L1 contribute to the accumulation of α-synuclein by downregulating its lysosomal degradation."

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"No clear substrates have been described for UCHL1 in vivo, but UCHL1 may deubiquitinate alpha-synuclein."
UCHL1 deubiquitinates tyrosine-phosphorylated SNCA on lysine. 1 / 1
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No evidence text available
UCHL1 deubiquitinates SNCA phosphorylated on S129 and tyrosine on lysine. 1 / 1
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No evidence text available
UCHL1 affects CDKN2A
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UCHL1 deubiquitinates CDKN2A. 4 / 4
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"Our previous work demonstrated that UCHL1 could activate the p14ARF-p53 signaling pathway by deubiquitinating p53 and p14ARF as well as ubiquitinating MDM2, which might be through its two opposing enzyme activities, hydrolase and ligase, further resulting in its tumor suppressive role in NPC tumorigenesis XREF_BIBR, XREF_BIBR."

trips
"These results indicate that UCHL1 could deubiquitinate p53 and p14(ARF) and ubiquitinate MDM2 for p53 stabilization to promote p53 signaling, thus involved in nasopharyngeal carcinoma pathogenesis, whereas it is frequently silenced in this tumor."

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"As P53 protein is regulated through ubiquitin dependent degradation in tumorigenesis, UCHL1 promotes p53 signaling by deubiquitinating p53 and p14 ARF and ubiquitinating MDM2 for further MDM2 degradation and p53 stabilization, thus involved in NPC pathogenesis as a functional TSG XREF_BIBR."

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"These results indicate that UCHL1 could deubiquitinate p53 and p14 (ARF) and ubiquitinate MDM2 for p53 stabilization to promote p53 signaling, thus involved in nasopharyngeal carcinoma pathogenesis, whereas it is frequently silenced in this tumor."
UCHL1 affects HIF1A
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UCHL1 deubiquitinates HIF1A. 3 / 3
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"We revealed that UCHL1 directly interacts with HIF-1α, mediates the deubiquitination of HIF-1α, and upregulates HIF-1 activity through the stabilization of HIF1α"

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"UCHL1 deubiquitinates HIF-1alpha protein and upregulates HIF-1 activity in murine breast cancer derived EMT6 cells."

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"Moreover, when the ubiquitination status of the HIF-1alpha protein was directly evaluated by performing immunoprecipitation assays, the knockdown of endogenous UCHL1 significantly increased HIF-1alpha ubiquitination (XREF_FIG and XREF_SUPPLEMENTARY)."
UCHL1 affects alpha-syn
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UCHL1 deubiquitinates alpha-syn. 1 / 1
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"55 The finding here that UCH-L1 can deubiquitinate alpha-syn is consistent with the established hydrolytic activity of UCHs."
Modified UCHL1 leads to the deubiquitination of alpha-syn. 1 / 1
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"In this study, we measured both mRNA and protein levels of UCHL1 in the whole brain of mice, and we found decreased expression of UCHL1 in LPL deficient mouse brain, which further led to enhanced ubiq[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
UCHL1 affects TGFB
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UCHL1 deubiquitinates TGFB. 1 / 1
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"In a separate study using a DUB activity profiling approach Liu et al. identified UCHL1 as a particularly active DUB in TNBC, proposing that UCHL1 also promotes TGF-β-induced TNBC metastasises by deubiquitinating and stabilising TGFβR1 and SMAD2 [54]."
UCHL1 deubiquitinates TGFB on R1. 1 / 1
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"In a separate study using a DUB activity profiling approach Liu et al. identified UCHL1 as a particularly active DUB in TNBC, proposing that UCHL1 also promotes TGF-β-induced TNBC metastasises by deubiquitinating and stabilising TGFβR1 and SMAD2 [54]."
UCHL1 affects SMAD2
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UCHL1 deubiquitinates SMAD2. 2 / 2
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"We also found that UCH-L1 interacts, deubiquitinates, and stabilizes SMAD2 and SMAD3."

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"In a separate study using a DUB activity profiling approach Liu et al. identified UCHL1 as a particularly active DUB in TNBC, proposing that UCHL1 also promotes TGF-β-induced TNBC metastasises by deubiquitinating and stabilising TGFβR1 and SMAD2 [54]."
UCHL1 affects PROX1
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UCHL1 deubiquitinates PROX1. 2 / 2
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"These studies provide theoretical support for our experimental results.The mechanism underlying our clinical findings may involve deubiquitination of PROX1 by UCHL1, as indicated in other studies."

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"We therefore hypothesized that PROX1 may be abnormally deubiquitinated by UCHL1 in SCNEC, resulting in lower ubiquitinated PROX1 levels and enhanced PROX1 activity."
UCHL1 affects CD36
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UCHL1 deubiquitinates CD36. 2 / 2
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"Deubiquitination of CD36 by UCHL1 promotes foam cell formation."

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"Deubiquitination of CD36 by UCHL1 promotes foam cell formation."
UCHL1 affects VEGF
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UCHL1 leads to the deubiquitination of VEGF. 1 / 1
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"We further showed that UCH-L1 increases ROS levels in HUVEC by deubiquitinating NOX4 in VEGF signaling."
UCHL1 affects UCHL1
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UCHL1 deubiquitinates UCHL1. 1 / 1
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"Interestingly, UCH-L1 catalyzes its own deubiquitination in an intramolecular manner, thereby regulating the lifetime of this modification."
UCHL1 affects TGFBR1
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UCHL1 deubiquitinates TGFBR1. 1 / 1
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"Besides, we found that recombinant UCHL1 protein is able to deubiquitinate TβRI and SMAD2 in vitro directly, and N-ethylmaleimide (NEM) treatment blocked this process by inhibiting UCHL1 DUB activity"
UCHL1 affects STUB1
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UCHL1 deubiquitinates STUB1. 1 / 1
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"No other tested DUB behaved in a similar manner : USP5 deubiquitinated Ub-CHIP in an unregulated manner, while UCHL-1 and UCHL-3 did not deubiquitinate Ub-CHIP under any conditions (XREF_FIG)."
UCHL1 affects SMAD3
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UCHL1 deubiquitinates SMAD3. 1 / 1
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"We also found that UCH-L1 interacts, deubiquitinates, and stabilizes SMAD2 and SMAD3."
UCHL1 affects SMAD2_3
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UCHL1 deubiquitinates SMAD2_3. 1 / 1
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"In a separate study Nagata et al. recently identified UCHL1 as an active DUB in lung cancer, where UCHL1 also supports TGF-β signalling by deubiquitinating and stabilising SMAD2/3 [67]."
UCHL1 affects SLC5A7
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UCHL1 deubiquitinates SLC5A7. 1 / 1
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"These data provide novel and potentially important evidence that UCHL1 may play a role in the regulation of cholinergic function by affecting CHT ubiquitination and degradation."
UCHL1 affects SLC1A2
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UCHL1 deubiquitinates SLC1A2. 1 / 1
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"Once inside the cell, ubiquitinated GLT-1 can be targeted to lysosomes for degradation, or it can return to the plasma membrane, being this process dependent on the deubiquitination of GLT-1 by UCH-L1 deubiquitinase (Martinez-Villarreal et al., 2012)."
UCHL1 affects RPTOR
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UCHL1 deubiquitinates RPTOR. 1 / 1
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"UCHL1 deubiquitinates Raptor, thereby destabilizing mTORC1 complex formation and shifting the balance toward mTORC2 dependent signaling."
UCHL1 affects NFKB1
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UCHL1 deubiquitinates NFKB1. 1 / 1
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"It was showed that UCH-L1 over-expression inhibited NF-κB activation and decrease IL-6 and IL-8 levels, while knockdown of it enhanced NF-κB activation and increase IL-6 and IL-8 levels during TNF-α treatment."
UCHL1 affects NCAM180
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Modified UCHL1 leads to the deubiquitination of NCAM180. 1 / 1
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"Overexpression of UCHL1 significantly decreased constitutive ubiquitination of NCAM180 and NCAM140 whereas inhibition of endogenous UCHL1 increased NCAM 's ubiquitination."
UCHL1 affects NCAM140
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Modified UCHL1 leads to the deubiquitination of NCAM140. 1 / 1
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"Overexpression of UCHL1 significantly decreased constitutive ubiquitination of NCAM180 and NCAM140 whereas inhibition of endogenous UCHL1 increased NCAM 's ubiquitination."
UCHL1 affects MHC I. 46 Another DUB
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UCHL1 deubiquitinates MHC I. 46 Another DUB. 1 / 1
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"Ubiquitin C-terminal hydrolase-L1 (UCH-L1) has been shown to positively regulate MHC I recycling and antigen cross presentation to CD8 T cells, although it is unclear whether UCH-L1 directly deubiquitinates MHC I. 46 Another DUB, USP14, facilitates MHC I restricted direct antigen presentation, particularly the peptides derived from defective ribosomal products (DRiPs)."
UCHL1 affects MAPT
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UCHL1 deubiquitinates MAPT. 1 / 1
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"Our results suggest that UCH-L1 may be associated with hippocampal MSF followed the epileptogenesis through mediating phosphorylation of tau."
UCHL1 affects IL6
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UCHL1 deubiquitinates IL6. 1 / 1
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"It was showed that UCH-L1 over-expression inhibited NF-κB activation and decrease IL-6 and IL-8 levels, while knockdown of it enhanced NF-κB activation and increase IL-6 and IL-8 levels during TNF-α treatment."
UCHL1 affects HSPA5
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UCHL1 leads to the deubiquitination of HSPA5. 1 / 1
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"Downregulation of UCHL1 by UCHL1 siRNA decreased poly-ubiquitination of GRP78."
UCHL1 affects HIF1
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UCHL1 deubiquitinates HIF1. 1 / 1
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"UCHL1 deubiquitinates HIF-1α protein [31], upregulates HIF-1 activity, and mediates cellular protection under hypoxia."
UCHL1 affects HDAC6
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UCHL1 deubiquitinates HDAC6. 1 / 1
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"By reducing the production of lysine (K63)-linked ubiquitin chains, UCH-L1 inhibition decreases HDAC6 deacetylase activity and attenuates the interaction of HDAC6 and tau protein, finally leading to tau aggresome formation impairment.?"
UCHL1 affects CXCL8
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UCHL1 deubiquitinates CXCL8. 1 / 1
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"It was showed that UCH-L1 over-expression inhibited NF-κB activation and decrease IL-6 and IL-8 levels, while knockdown of it enhanced NF-κB activation and increase IL-6 and IL-8 levels during TNF-α treatment."
UCHL1 affects CTNNB1
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UCHL1 deubiquitinates CTNNB1. 1 / 1
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"Although, it is unlikely UCH-L1 directly deubiquitinates beta-catenin [XREF_BIBR, XREF_BIBR], these observations suggest UCH-L1 may convey its oncogenic function through Wnt signaling pathways."
UCHL1 affects COPS5
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UCHL1 deubiquitinates COPS5. 1 / 1
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"UCH-L1 colocalizes with Jab1 sending p27Kip1?to proteasomal degradation, prevents senescence, and ensures proper somatic cell division"
UCHL1 affects BACE1
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UCHL1 deubiquitinates BACE1. 1 / 1
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"Whether UCHL1, with its physically constrained catalytic site, can deubiquitinate BACE1 and how this would accelerate its degradation is presently unclear."
UCHL1 affects APP
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UCHL1 leads to the deubiquitination of APP. 1 / 1
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"To investigate whether UCHL1 promotes the ubiquitination and degradation of APP by regulating the free ubiquitin pool, Haw cells were co-transfected with pZ-UCHL1-st and ubiquitin expression plasmid pCW7."