IndraLab

Statements


| 4 16
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sparser
"While the interaction between AMSH-LP with distal Ub domain is critical for binding affinity, the interaction of AMSH-LP with proximal Ub is critical for catalysis."

sparser
"Sato et al. resolved the crystal structure of AMSH-LP E292A -ubiquitin complex (PDB ID: 2ZNV) from H. sapiens and proposed the catalytic mechanism of JAMMs, which was probably similar to that of thermolysin ( xref H) [ xref , xref ]."

sparser
"Cys282 is next to the active site, and in fact it makes a van der Waals contact with Leu73 of the distal ubiquitin in the structure of AMSH-LP DUB domain bound to Lys63-linked ubiquitin dimer. xref In the disulfide orientation, however, as seen in the model of AMSH244 bound to Lys63-linked diubiquitin (please see below), the contact is lost."

sparser
"Interestingly, in the structure of AMSH-LP DUB domain bound to Lys63-linked ubiquitin dimer, the corresponding residue pair is still seen in a similar position as the substrate-free form, xref suggesting that these residues close the active site during catalysis as well, perhaps to help position the scissile peptide bond or stabilize the transition state or both."

sparser
"It is known that Val328 and Phe332 of AMSH-LP interact with ubiquitin Ile44, but structural comparison shows that the equivalent positions in Rpn11 (Val83 and Phe87) are oriented in an opposite direction from AMSH-LP ( xref B)."

sparser
"The Oγ atom of AMSH’s Ser346 is pointing away from Glu64 of the proximal ubiquitin ( xref ), contrary to the equivalent residue in both free and ubiquitin-bound AMSH-LP, which are at distances of 2.4Å and 2.6Å from proximal ubiquitin’s Glu64, respectively."

reach
"A structural study revealed that AMSH-LP directly binds Ub molecules to specifically cleave Ub chains ( Sato et al., 2008 )."

sparser
"A structural study revealed that AMSH-LP directly binds Ub molecules to specifically cleave Ub chains ( Sato et al., 2008 )."

sparser
"The structural basis for this recognition was elucidated with the homologous protein, AMSH-LP, bound to Lys63-linked dimer of ubiquitin ( xref )."

sparser
"Using the AMSH-LP E292A -ubiquitin complex (PDB ID: 2ZNV) from Homo sapiens as an example, the C-terminal tail of the distal ubiquitin moiety binds the active site cleft and is stabilized with the Ins-1 segment by extensive hydrophobic interactions and hydrogen bonds, thus facilitating a scissile isopeptide bond for hydrolysis ( xref A,C) [ xref ]."
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sparser
"The structure was determined by molecular replacement using a model of Sst2–K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref )) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry (Table xref )."

sparser
"The overall three-dimensional fold of Sst2 Δ220 –K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 Å of Cα atoms over 266 residues from both Sst2 and K63-Ub 2 ."

sparser
"Figure xref d shows the superposition of Sst2 Δ220 –K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref ))."

sparser
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2 ."
Rpn11 binds STAMBPL1 and Ubiquitin. 1 / 1
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sparser
"Rpn11 and AMSH-LP bind ubiquitin in a similar orientation, but their interaction with the Ile44 hydrophobic patch is quite different."

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
STAMBPL1 binds patch and Ubiquitin. 1 / 1
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sparser
"AMSH-LP binds the distal ubiquitin via its Leu8/Ile44/Val70 hydrophobic patch conserved in NEDD8."
STAMBPL1 inhibits Ubiquitin.
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reach
"Particularly, sites 251-436 of STAMBPL1 interact with sites 228-605 of NRF2, facilitating DUB activity and eliminating ubiquitin molecules attached to NRF2, thus protecting it from proteasome-mediated degradation."
STAMBPL1 increases the amount of Ubiquitin.
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STAMBPL1 increases the amount of Ubiquitin. 1 / 1
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reach
"Since our data clearly indicate that STAMBPL1 affects Snail stability via the proteasome, we assessed if the levels of the main ubiquitin ligases or DUBs involved in the regulation of Snail half-life are modulated by STAMBPL1."
STAMBPL1 activates Ubiquitin.
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reach
"The observation that AMSH-LP causes partial cleavage of the first ubiquitin attached to some proteins has been noted previously [24]."
STAMBPL1 affects CFLAR
1 | 1 14 7
STAMBPL1 binds CFLAR.
1 | 3 7
1 | 3 5

reach
"Specifically, when STAMBPL1 and c-FLIP bound together, STAMBPL1 deubiquitylated c-FLIP."

sparser
"Specifically, when STAMBPL1 and c-FLIP bound together, STAMBPL1 deubiquitylated c-FLIP."

reach
"STAMBPL1 bound to c-FLIP and deubiqutylated c-FLIP protein."

sparser
"As DUBs remove ubiquitin chains by binding to the target protein and inhibiting ubiquitination, we performed an immunoprecipitation (IP) assay using the STAMBPL1 antibody and observed that endogenous STAMBPL1 interacts with c-FLIP ( xref C)."

sparser
"Additionally, we observed that STAMBPL1 endogenously interacts with c-FLIP ( xref C)."

sparser
"STAMBPL1 bound to c-FLIP and deubiqutylated c-FLIP protein."

sparser
"In addition, STAMBPL1 also bound endogenous c-FLIP in Caki cells ( xref D)."

reach
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
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sparser
"Thus, these data reveal that STAMBPL1 directly interacts with survivin and c-FLIP, resulting in stabilization of proteins."

sparser
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
STAMBPL1 activates CFLAR.
| 1 5
| 1 5

reach
"Knockdown of STAMBPL1 reduced survivin and c-FLIP protein levels, while overexpression of STAMBPL1 inhibited honokinol induced survivin and c-FLIP degradation."

reach
"Our findings provided that honokiol could overcome TRAIL resistance through survivin and c-FLIP degradation induced by inhibition of STAMBPL1 expression."

reach
"STAMBPL1 stabilizes XIAP to inhibit apoptosis in prostate cancer cells (Chen et al., 2019) and confers resistance to honokiol-induced apoptosis in various cancer cell types by stabilizing Survivin and c-FLIP (Woo et al., 2019)."

reach
"Tubeimoside-1 Enhances TRAIL-Induced Apoptotic Cell Death through STAMBPL1-Mediated c-FLIP Downregulation."

eidos
"Mechanistically , TBMS-1 destabilized c-FLIP expression by downregulating STAMBPL1 , a deubiquitinase ( DUB ) ."

reach
"STAMBPL1 overexpression markedly prevented apoptosis induced by TBMS-1 and TRAIL combination therapy as well as c-FLIP degradation (Figure 4G)."
STAMBPL1 inhibits CFLAR.
| 3
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reach
"This is achieved through the downregulation of anti-apoptotic proteins survivin and cFLIP, which are degraded by the deubiquitinase STAMBPL1."

reach
"This study demonstrated that TBMS-1 sensitizes cancer cells to the apoptosis effect of TRAIL via STAMBPL1-dependent downregulation of c-FLIP."

reach
"We previously reported the involvement of STAMBPL1-mediated c-FLIP degradation in TRAIL sensitization and showed that STABMPL1 overexpression reduces c-FLIP ubiquitination [19]."
STAMBPL1 increases the amount of CFLAR.
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STAMBPL1 increases the amount of CFLAR. 1 / 1
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reach
"However, considering the observation that STAMBPL1 knockdown reduced c-FLIP expression, we proposed c-FLIP as a new substrate of STAMBPL1."
STAMBPL1 deubiquitinates CFLAR.
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STAMBPL1 deubiquitinates CFLAR. 1 / 1
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reach
"Specifically, when STAMBPL1 and c-FLIP bound together, STAMBPL1 deubiquitylated c-FLIP."
STAMBPL1 decreases the amount of CFLAR.
| 1
STAMBPL1 decreases the amount of CFLAR. 1 / 1
| 1

reach
"Knockdown of STAMBPL1 reduced survivin and c-FLIP protein levels, while overexpression of STAMBPL1 inhibited honokinol induced survivin and c-FLIP degradation."
STAMBPL1 affects SESN2
2 | 9 11
STAMBPL1 binds SESN2.
2 | 2 11
2 | 2 11

sparser
"Inhibiting the interaction between STAMBPL1 and Sestrin2 thereby inactivating mTORC1 using a cell-permeable peptide could be a potential treatment approach for gastric and colorectal cancers [ xref ]."

reach
"The binding of RNF167 and STAMBPL1 to Sestrin2 is not mutually exclusive, as the interaction of one protein with Sestrin2 was not affected by deficiency or overexpression of the other protein ( Figure[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Importantly, we demonstrated that disrupting the STAMBPL1-Sestrin2 interaction with a cell-penetrating peptide might serve as a potential strategy for colon cancer therapy."

sparser
"Moreover, a cell-permeable peptide that blocks STAMBPL1-Sestrin2 interaction suppresses mTOR signaling and anchorage-independent cell growth of human colon cancer cells."

reach
"Inhibiting the interaction between STAMBPL1 and Sestrin2 thereby inactivating mTORC1 using a cell-permeable peptide could be a potential treatment approach for gastric and colorectal cancers [141]."

sparser
"Next, we tested whether a cell-permeable peptide disrupting STAMBPL1-Sestrin2 interaction may suppress cancer development."

sparser
"Deleting the USP8_dimer domain or the linker region between the USP8_dimer domain and the JAB domain (UJ_linker) suppressed STAMBPL1-Sestrin2 interaction ( Figures S10 A and S10B)."

sparser
"Therefore, disrupting the STAMBPL1-Sestrin2 interaction with a cell-permeable peptide may be a promising therapeutic strategy for gastric and colorectal cancers."

sparser
"A cell-permeable peptide disrupting STAMBPL1-Sestrin2 interaction can inhibit mTORC1 signaling and suppress colon cancer cell growth ( Figure 7 F)."

sparser
"In contrast, changes in arginine levels did not affect RNF167-Sestrin2 or STAMBPL1-Sestrin2 interaction ( Figures S4 C and S4D)."
STAMBPL1 deubiquitinates SESN2.
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STAMBPL1 deubiquitinates SESN2. 4 / 4
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reach
"To test whether STAMBPL1 or STAMBP plays the dominant role in deubiquitinating Sestrin2 and regulating mTORC1 signaling, we knocked out STAMBPL1 on its own or in combination with STAMBP knockdown."

reach
"Interestingly, Wang et al. reported that gastric cancer and colorectal cancer downregulate an E3 ubiquitin ligase RING finger protein 167 (RNF167) and upregulate a deubiquitinase STAMBPL1 that can prevent the ubiquitination of Sestrin2, decrease Sestrin2-GATOR2 interaction and increase mTORC1 signaling [119]."

reach
"Deficiency of STAMBPL1 promoted Sestrin2 ubiquitination ( Figures 2 E and S3 H–S3J), prevented lysosomal localization of mTORC1 upon amino acid sufficiency and suppressed mTORC1 activity ( Figures 2 F[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"These results indicate that STAMBPL1 plays a major role in deubiquitinating Sestrin2."
Mutated STAMBPL1 leads to the deubiquitination of SESN2. 1 / 1
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reach
"Consistent with the above results, endogenous K405Rfs ∗ 21 mutation of STAMBPL1 promoted the deubiquitination of Sestrin2 and activated mTORC1 signaling ( Figures 6 D and 6E)."
STAMBPL1 ubiquitinates SESN2.
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STAMBPL1 leads to the ubiquitination of SESN2. 1 / 1
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reach
"Consistently, knockout of STAMBPL1 and subsequent increase in Sestrin2 ubiquitination markedly inhibited xenograft tumor growth [119]."
STAMBPL1 decreases the amount of SESN2.
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STAMBPL1 decreases the amount of SESN2. 1 / 1
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reach
"Knockout of STAMBPL1 greatly elevated the polyubiquitination level of Sestrin2 and suppressed mTORC1 activity ( Figures S3 D and S3E)."
| 1 16

sparser
"While the interaction between AMSH-LP with distal Ub domain is critical for binding affinity, the interaction of AMSH-LP with proximal Ub is critical for catalysis."

sparser
"Sato et al. resolved the crystal structure of AMSH-LP E292A -ubiquitin complex (PDB ID: 2ZNV) from H. sapiens and proposed the catalytic mechanism of JAMMs, which was probably similar to that of thermolysin ( xref H) [ xref , xref ]."

sparser
"Cys282 is next to the active site, and in fact it makes a van der Waals contact with Leu73 of the distal ubiquitin in the structure of AMSH-LP DUB domain bound to Lys63-linked ubiquitin dimer. xref In the disulfide orientation, however, as seen in the model of AMSH244 bound to Lys63-linked diubiquitin (please see below), the contact is lost."

sparser
"Interestingly, in the structure of AMSH-LP DUB domain bound to Lys63-linked ubiquitin dimer, the corresponding residue pair is still seen in a similar position as the substrate-free form, xref suggesting that these residues close the active site during catalysis as well, perhaps to help position the scissile peptide bond or stabilize the transition state or both."

sparser
"It is known that Val328 and Phe332 of AMSH-LP interact with ubiquitin Ile44, but structural comparison shows that the equivalent positions in Rpn11 (Val83 and Phe87) are oriented in an opposite direction from AMSH-LP ( xref B)."

sparser
"The Oγ atom of AMSH’s Ser346 is pointing away from Glu64 of the proximal ubiquitin ( xref ), contrary to the equivalent residue in both free and ubiquitin-bound AMSH-LP, which are at distances of 2.4Å and 2.6Å from proximal ubiquitin’s Glu64, respectively."

reach
"A structural study revealed that AMSH-LP directly binds Ub molecules to specifically cleave Ub chains ( Sato et al., 2008 )."

sparser
"A structural study revealed that AMSH-LP directly binds Ub molecules to specifically cleave Ub chains ( Sato et al., 2008 )."

sparser
"The structural basis for this recognition was elucidated with the homologous protein, AMSH-LP, bound to Lys63-linked dimer of ubiquitin ( xref )."

sparser
"Using the AMSH-LP E292A -ubiquitin complex (PDB ID: 2ZNV) from Homo sapiens as an example, the C-terminal tail of the distal ubiquitin moiety binds the active site cleft and is stabilized with the Ins-1 segment by extensive hydrophobic interactions and hydrogen bonds, thus facilitating a scissile isopeptide bond for hydrolysis ( xref A,C) [ xref ]."
| 4

sparser
"The structure was determined by molecular replacement using a model of Sst2–K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref )) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry (Table xref )."

sparser
"The overall three-dimensional fold of Sst2 Δ220 –K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 Å of Cα atoms over 266 residues from both Sst2 and K63-Ub 2 ."

sparser
"Figure xref d shows the superposition of Sst2 Δ220 –K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref ))."

sparser
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2 ."
Rpn11 binds STAMBPL1 and Ubiquitin. 1 / 1
| 1

sparser
"Rpn11 and AMSH-LP bind ubiquitin in a similar orientation, but their interaction with the Ile44 hydrophobic patch is quite different."

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
STAMBPL1 binds patch and Ubiquitin. 1 / 1
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sparser
"AMSH-LP binds the distal ubiquitin via its Leu8/Ile44/Val70 hydrophobic patch conserved in NEDD8."
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reach
"In response to genotoxic stress, the degradation of STAMBPL1 augments apoptotic cell death."

reach
"Moreover, Chen et al. indicated that STAMBPL1 depletion increases cell death 72 h after shRNA treatment; however, our data did not show STAMBPL1 siRNA-induced apoptosis after treatment for 24 h (Figure 4G)."

reach
"We observed activation of caspase-9, -3 and -7, but not caspase-8, in STAMBPL1 silencing-induced apoptosis."

reach
"Here we show evidence that STAMBPL1 regulates X-linked inhibitor of apoptosis protein (XIAP) stability, thus modulates apoptosis in prostate cancer cells.XIAP is one of the Inhibitor of Apoptosis Prot[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Apoptosis of CRC was induced by silence of STAMBPL1."

reach
"STAMBPL1 overexpression markedly prevented apoptosis induced by TBMS-1 and TRAIL combination therapy as well as c-FLIP degradation (Figure 4G)."

reach
"STAMBPL1 depletion was found to induce apoptosis by promoting the degradation of the protein XIAP, suggesting that targeting STAMBPL1 might offer a promising therapeutic strategy for prostate cancer [23]."

reach
"We designed experiments to show that the percentage of apoptotic cells in A549 STAMBPL1 SI group (4.89% ± 0.33%, p<0.05) and H1299 STAMBPL1 SI (9.18% ± 0.51%, p<0.001) group increased significantly compared with A549 STAMBPL1 NC group (3.33% ± 0.41%) and H1299 STAMBPL1 NC group (2.91% ± 0.24%) (Fig. 2G, p<0.05, p<0.001), which proved that STAMBPL1 could reduce apoptosis of LUAD cells."

reach
"STAMBPL1 reduces apoptosis of LUAD cells."

eidos
"In response to genotoxic stress , the degradation of STAMBPL1 augments apoptotic cell death ."
| 4

reach
"Mechanistically, downregulation of STAMBPL1 upon H. pylori infection has been shown to promote apoptosis by circumventing the deubiquitinylation and hence stabilization of the antiapoptotic protein su[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"STAMBPL1 stabilizes XIAP to inhibit apoptosis in prostate cancer cells (Chen et al., 2019) and confers resistance to honokiol-induced apoptosis in various cancer cell types by stabilizing Survivin and c-FLIP (Woo et al., 2019)."

reach
"Further, we demonstrated the involvement of STAMBPL1 in combined treatment-induced apoptosis using an overexpression or depletion system."

reach
"Recent studies have shown that excessive ROS generated in H. pylori infection induces direct degradation of STAMBPL1 by cullin 1-RING ubiquitin ligase and the 26S proteasome, while STAMBPL1 can lead to cell apoptosis by reducing the anti-apoptotic protein survivin (Chaithongyot and Naumann, 2022)."
STAMBPL1 affects BIRC5
3 | 13 4
STAMBPL1 binds BIRC5.
3 | 1 4
3 | 1 2

sparser
"However, although cepharanthine reduces STAMBPL1 expression and overexpression of STAMBPL1 inhibits cepharanthine-mediated downregulation of survivin, interaction between STAMBPL1 and survivin was not investigated."

reach
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."

sparser
"As shown in xref C, we detected the interaction between STAMBPL1 and survivin."
| 2

sparser
"Thus, these data reveal that STAMBPL1 directly interacts with survivin and c-FLIP, resulting in stabilization of proteins."

sparser
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
STAMBPL1 activates BIRC5.
| 5
| 5

reach
"Mechanistically, downregulation of STAMBPL1 upon H. pylori infection has been shown to promote apoptosis by circumventing the deubiquitinylation and hence stabilization of the antiapoptotic protein su[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Knockdown of STAMBPL1 reduced survivin and c-FLIP protein levels, while overexpression of STAMBPL1 inhibited honokinol induced survivin and c-FLIP degradation."

reach
"Our findings provided that honokiol could overcome TRAIL resistance through survivin and c-FLIP degradation induced by inhibition of STAMBPL1 expression."

reach
"STAMBPL1 stabilizes XIAP to inhibit apoptosis in prostate cancer cells (Chen et al., 2019) and confers resistance to honokiol-induced apoptosis in various cancer cell types by stabilizing Survivin and c-FLIP (Woo et al., 2019)."

reach
"Recent studies have shown that excessive ROS generated in H. pylori infection induces direct degradation of STAMBPL1 by cullin 1-RING ubiquitin ligase and the 26S proteasome, while STAMBPL1 can lead to cell apoptosis by reducing the anti-apoptotic protein survivin (Chaithongyot and Naumann, 2022)."
STAMBPL1 inhibits BIRC5.
| 4
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reach
"This is achieved through the downregulation of anti-apoptotic proteins survivin and cFLIP, which are degraded by the deubiquitinase STAMBPL1."

reach
"Honokiol Enhances TRAIL Mediated Apoptosis through STAMBPL1 Induced Survivin and c-FLIP Degradation."

reach
"Ectopic expression of STAMBPL1 significantly inhibited CEP induced survivin downregulation (XREF_FIG F)."

reach
"Next, we investigated whether overexpression of STAMBPL1 prevents CEP induced survivin downregulation."
STAMBPL1 increases the amount of BIRC5.
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STAMBPL1 increases the amount of BIRC5. 2 / 2
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reach
"Cepharanthine Enhances TRAIL Mediated Apoptosis Through STAMBPL1 Mediated Downregulation of Survivin Expression in Renal Carcinoma Cells."

reach
"Exogenous expression of STAMBPL1 increased the percentage of cells in the S phase and upregulated the expressions of CyclinD1 and Survivin."
STAMBPL1 decreases the amount of BIRC5.
| 1
STAMBPL1 decreases the amount of BIRC5. 1 / 1
| 1

reach
"Knockdown of STAMBPL1 reduced survivin and c-FLIP protein levels, while overexpression of STAMBPL1 inhibited honokinol induced survivin and c-FLIP degradation."
SESN2 affects STAMBPL1
2 | 2 11
2 | 2 11

sparser
"Inhibiting the interaction between STAMBPL1 and Sestrin2 thereby inactivating mTORC1 using a cell-permeable peptide could be a potential treatment approach for gastric and colorectal cancers [ xref ]."

reach
"The binding of RNF167 and STAMBPL1 to Sestrin2 is not mutually exclusive, as the interaction of one protein with Sestrin2 was not affected by deficiency or overexpression of the other protein ( Figure[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Importantly, we demonstrated that disrupting the STAMBPL1-Sestrin2 interaction with a cell-penetrating peptide might serve as a potential strategy for colon cancer therapy."

sparser
"Moreover, a cell-permeable peptide that blocks STAMBPL1-Sestrin2 interaction suppresses mTOR signaling and anchorage-independent cell growth of human colon cancer cells."

reach
"Inhibiting the interaction between STAMBPL1 and Sestrin2 thereby inactivating mTORC1 using a cell-permeable peptide could be a potential treatment approach for gastric and colorectal cancers [141]."

sparser
"Next, we tested whether a cell-permeable peptide disrupting STAMBPL1-Sestrin2 interaction may suppress cancer development."

sparser
"Deleting the USP8_dimer domain or the linker region between the USP8_dimer domain and the JAB domain (UJ_linker) suppressed STAMBPL1-Sestrin2 interaction ( Figures S10 A and S10B)."

sparser
"Therefore, disrupting the STAMBPL1-Sestrin2 interaction with a cell-permeable peptide may be a promising therapeutic strategy for gastric and colorectal cancers."

sparser
"A cell-permeable peptide disrupting STAMBPL1-Sestrin2 interaction can inhibit mTORC1 signaling and suppress colon cancer cell growth ( Figure 7 F)."

sparser
"In contrast, changes in arginine levels did not affect RNF167-Sestrin2 or STAMBPL1-Sestrin2 interaction ( Figures S4 C and S4D)."
TRAF2 affects STAMBPL1
| 6 6
TRAF2 binds STAMBPL1.
| 3 6
| 3 6

sparser
"The 251–436 sites of STAMBPL1 particularly interact with the 294–496 sites of TRAF2, thereby exerting the function of DUB and removing the ubiquitin molecules attached to TRAF2."

reach
"The CO-IP experiment was further utilized to identify the interaction of STAMBPL1 and TRAF2 in HCCLM3 and Hep3B cell line, and the result showed that STAMBPL1 can interact with TRAF2 in both HCCLM3 and Hep3B cell line (Fig. 6B, C)."

reach
"The interaction between the TRAF2 (1-348), TRAF2 (74-496) fragments, and STAMBPL1 was verified (Fig. 8E)."

sparser
"STAMBPL1 directly interacts with TRAF2 and stabilize its protein stability."

sparser
"The CO-IP experiment was further utilized to identify the interaction of STAMBPL1 and TRAF2 in HCCLM3 and Hep3B cell line, and the result showed that STAMBPL1 can interact with TRAF2 in both HCCLM3 and Hep3B cell line (Fig.  xref B, C)."

sparser
"The specific binding sites of interaction between STAMBPL1 and TRAF2."

reach
"The specific binding sites of interaction between STAMBPL1 and TRAF2."

sparser
"Simultaneously, through IP experiments, it was further discovered that STAMBPL1 can interact with the TRAF2 (1-348), TRAF2 (74-496) fragments and undergo deubiquitination modification (Fig.  xref F)."

sparser
"IP assays confirmed the interaction between the STAMBPL1 (251-436) fragment and TRAF2 (Fig.  xref D)."
TRAF2 inhibits STAMBPL1.
| 3
| 3

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"TRAF2 reversed the tumorigenic activity of STAMBPL1 in HCC cells."

reach
"The results from the colony formation assay suggested that TRAF2 can reverse the pro-proliferative ability of STAMBPL1 in HCC cells (Fig. 9E–F)."

reach
"TRAF2 can reverse the tumorigenic activity of STAMBPL1 in vitro at the same time (Fig. 11M–R)."

reach
"Additionally, the suppression of STAMBPL1 inhibits tumor growth and the proliferation in colorectal and gastric cancer cells [20,21]."

reach
"These findings highlight the diverse roles that deubiquitinases play in HCC, either by promoting or suppressing the disease, and suggest that they could be potential targets for therapeutic intervention in HCC.In the study, we identified one oncogene gene (STAMBPL1) which promote the proliferation and metastasis of HCC."

reach
"Functionally, STAMBPL1 notably enhances CCA cell proliferation and metastasis while impeding ferroptosis."

reach
"Knockdown of STAMBPL1 reduced cell viability of CRC and suppressed the proliferation, invasion, and migration."

reach
"CCK-8 assays revealed that the inhibition of STAMBPL1 could reduce the proliferation of HCCLM3 and Hep3B (Fig. 3G)."

reach
"STAMBPL1 has been reported to promote cell proliferation and function as an oncogenic protein in gastric cancer ( Yu et al., 2019 )."

reach
"Following STAMBPL1 knockdown by short hairpin RNA (sh)STAMBPL1, cell proliferation was significantly suppressed, the cell apoptosis rate was significantly upregulated, and the numbers of invasive AGS cells and the AGS wound healing rate were significantly decreased (P<0.01 and P<0.001, respectively), compared with those in the shControl group."

reach
"These results suggested that AGS cell proliferation was suppressed by STAMBPL1 knockdown."

reach
"STAMBPL1 promotes the ability of cell cloning and proliferation in LUAD."

reach
"Functionally, STAMBPL1 significantly promoted HCC cells proliferation and metastasis, and it interacts with TRAF2 and stabilize it via the deubiquitination at the K63 residue."

reach
"Furthermore, knockdown of STAMBPL1 by shSTAMBPL1 resulted in STAMBPL1 downregulation, which in turn decreased the cell proliferation of AGS gastric cancer cells, increasing cell apoptosis, cell invasion and migration in vitro."

reach
"In addition, the results from the colony formation experiment demonstrated that the downregulation of STAMBPL1 suppressed the proliferation of hepatocellular carcinoma cells (Fig. 3H)."
STAMBPL1 affects STAMBP
2 | 4 6
STAMBPL1 binds STAMBP.
2 | 2 5
2 | 2 4

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30)."

sparser
"Unlike the monomer of AMSH or AMSH-LP with inherent DUB activity, BRCC36 must form a complex with other subunits to specifically cleave K63-linked polyubiquitin chains."

sparser
"To quantify the interaction of the UbVs with STAMBP and STAMBPL1 and to validate the key residues responsible for the tight binding, we used isothermal titration calorimetry (ITC) to measure the interaction thermodynamics ( xref )."

sparser
"Hameed et al. developed a zinc chelating ABP that forms a tight complex with STAMBP and STAMBPL1 and inhibits the activity of the proteasomal DUB Rpn11 (PSMD14 in humans)."

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30) ."
| DOI

sparser
"Hence, it is possible that AMSH and AMSH-LP might be associated with a proteasome or other unknown large protein complex in the nucleus through their MPN domain."

sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
STAMBPL1 inhibits STAMBP.
| 2
| 2

reach
"Next, we compared the K63-diUb (FRET) cleavage inhibition by the UbVs with STAMBP JAMM to the inhibition of fulllength STAMBP activated by STAM protein and full-length STAMBPL1."
| DOI

reach
"Next, we compared the K63-diUb (FRET) cleavage inhibition by the UbVs with STAMBP to the inhibition of full-length STAMBP activated by STAM protein and full-length STAMBPL1."
STAMBPL1 activates STAMBP.
| 1
| 1

sparser
"Next, we compared the K63-diUb (FRET) cleavage inhibition by the UbVs with STAMBP JAMM to the inhibition of full-length STAMBP activated by STAM protein and full-length STAMBPL1."
| 12
STAMBPL1 activates Neoplasms.
| 11
| 10

reach
"Additionally, the suppression of STAMBPL1 inhibits tumor growth and the proliferation in colorectal and gastric cancer cells [20,21]."

reach
"Therefore, we investigated whether STAMBPL1 promotes tumor angiogenesis."

reach
"Deletion of STAMBPL1 or correction of the heterozygous STAMBPL1 mutation in a human colon cancer cell line suppresses xenograft tumor growth."

reach
"STAMBPL1 knockout or correction of the heterozygous STAMBPL1 mutation in a human colon cancer cell could suppress xenograft tumor growth [24]."

reach
"Notably, STAMBPL1 overexpression increased lipid accumulation in HCC cells and xenograft tumors."

reach
"STAMBPL1 knockout significantly suppressed mTORC1 activity, anchorage-independent cell growth, and xenograft tumor growth ( Figures 5 F–5J and S8 C–S8J) in all three cell lines."

reach
"Besides, upregulation of STAMBPL1 could significantly enhance the tumor growth rate and volume in nude mice (Fig. 11E–H)."

reach
"All these results hinted that the STAMBPL1 positively mediated tumor growth in vitro."

reach
"Suppression of STAMBPL1 function inhibits tumor growth and provides a potential option for colorectal cancer therapy."

reach
"Knocking down STAMBPL1 inhibited the growth of HCC1806 xenografts in mice (Figure 3G–I) and decreased the number of microvessels in tumor tissue (Figure 3J, K), indicating that STAMBPL1 promoted tumor angiogenesis."
Mutated STAMBPL1 activates Neoplasms. 1 / 1
| 1

reach
"STAMBPL1 knockout or correction of the heterozygous STAMBPL1 mutation in a human colon cancer cell could suppress xenograft tumor growth [24]."
STAMBPL1 inhibits Neoplasms.
| 1
| 1

reach
"Tumor growth of CRC was also suppressed by the silence of STAMBPL1."
STAMBPL1 affects FOXO1
| 3 9
STAMBPL1 binds FOXO1.
| 9

sparser
"The study also shows that STAMBPL1 interacts with FOXO1 to promote GRHL3 transcription, which in turn activates HIF1A."

sparser
"Because the authors find that STAMBPL1 and FOXO1 interact, they suggest that STAMBPL1 may promote the formation of an active transcriptional complex containing FOXO1, perhaps by facilitating the recruitment of transcriptional coactivators."

sparser
"The results of the GST pull-down assay indicated that the N-terminus (1–140 aa) of STAMBPL1 interacted with FOXO1 ( xref )."

sparser
"Since the interaction sites of STAMBPL1-FOXO1 have been mapped, a mutant disrupting the interaction would provide better insight into how STAMBPL1 promotes GRHL3 transcription by interacting with FOXO1."

sparser
"Additionally, we found that STAMBPL1 interacts with FOXO1 to facilitate the transcription of GRHL3/HIF1α/VEGFA."

sparser
"This interaction between STAMBPL1 and FOXO1 does not necessarily affect the binding of FOXO1 with DNA, including the GRHL3 promoter."

sparser
"Additionally, an immunoprecipitation assay revealed an interaction between the FOXO1 protein (250–596 aa) and STAMBPL1 ( xref )."

sparser
"They demonstrate that STAMBPL1 interacts with FOXO1, define the required domains in each protein, and illustrate that this interaction facilitates FOXO1 transcriptional factor activity, which then activates GRHL3/HIF1a/VEGFA signaling."

sparser
"Furthermore, it would be important to determine whether the STAMBPL1-FOXO1 interaction is essential for GRHL3 transcription."
STAMBPL1 activates FOXO1.
| 3
| 3

reach
"However, the mechanism by which STAMBPL1 activates the transcriptional activity of FOXO1 has not been elucidated."

reach
"This suggests that STAMBPL1 enhances the transcriptional activity of FOXO1 on GRHL3 by interacting with nuclear FOXO1."

reach
"STAMBPL1 was shown to increase the transcriptional activation of FOXO1 at the GRHL3 promoter (Figure 7K), and it also bound to the GRHL3 promoter which could be disrupted by FOXO1 knockdown (Figure 7L)."
CFLAR affects STAMBPL1
1 | 4 7
CFLAR binds STAMBPL1.
1 | 3 7
1 | 3 5

reach
"Specifically, when STAMBPL1 and c-FLIP bound together, STAMBPL1 deubiquitylated c-FLIP."

sparser
"Specifically, when STAMBPL1 and c-FLIP bound together, STAMBPL1 deubiquitylated c-FLIP."

reach
"STAMBPL1 bound to c-FLIP and deubiqutylated c-FLIP protein."

sparser
"As DUBs remove ubiquitin chains by binding to the target protein and inhibiting ubiquitination, we performed an immunoprecipitation (IP) assay using the STAMBPL1 antibody and observed that endogenous STAMBPL1 interacts with c-FLIP ( xref C)."

sparser
"Additionally, we observed that STAMBPL1 endogenously interacts with c-FLIP ( xref C)."

sparser
"STAMBPL1 bound to c-FLIP and deubiqutylated c-FLIP protein."

sparser
"In addition, STAMBPL1 also bound endogenous c-FLIP in Caki cells ( xref D)."

reach
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
| 2

sparser
"Thus, these data reveal that STAMBPL1 directly interacts with survivin and c-FLIP, resulting in stabilization of proteins."

sparser
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
CFLAR inhibits STAMBPL1.
| 1
| 1

reach
"Moreover, STAMBPL1 knockdown markedly increased sensitivity to TRAIL by destabilizing c-FLIP."
STAMBPL1 affects TRAF2
| 5 6
STAMBPL1 binds TRAF2.
| 3 6
| 3 6

sparser
"The 251–436 sites of STAMBPL1 particularly interact with the 294–496 sites of TRAF2, thereby exerting the function of DUB and removing the ubiquitin molecules attached to TRAF2."

reach
"The CO-IP experiment was further utilized to identify the interaction of STAMBPL1 and TRAF2 in HCCLM3 and Hep3B cell line, and the result showed that STAMBPL1 can interact with TRAF2 in both HCCLM3 and Hep3B cell line (Fig. 6B, C)."

reach
"The interaction between the TRAF2 (1-348), TRAF2 (74-496) fragments, and STAMBPL1 was verified (Fig. 8E)."

sparser
"STAMBPL1 directly interacts with TRAF2 and stabilize its protein stability."

sparser
"The CO-IP experiment was further utilized to identify the interaction of STAMBPL1 and TRAF2 in HCCLM3 and Hep3B cell line, and the result showed that STAMBPL1 can interact with TRAF2 in both HCCLM3 and Hep3B cell line (Fig.  xref B, C)."

sparser
"The specific binding sites of interaction between STAMBPL1 and TRAF2."

reach
"The specific binding sites of interaction between STAMBPL1 and TRAF2."

sparser
"Simultaneously, through IP experiments, it was further discovered that STAMBPL1 can interact with the TRAF2 (1-348), TRAF2 (74-496) fragments and undergo deubiquitination modification (Fig.  xref F)."

sparser
"IP assays confirmed the interaction between the STAMBPL1 (251-436) fragment and TRAF2 (Fig.  xref D)."
STAMBPL1 decreases the amount of TRAF2.
| 1
STAMBPL1 decreases the amount of TRAF2. 1 / 1
| 1

reach
"Meanwhile, the ubiquitination level of TRAF2 was also detected in control group and shSTAMBPL1 group, and it showed that the inhibition of STAMBPL1 can increasing the ubiquitination level of TRAF2 in both HCCLM3 and Hep3B cell (Fig. 7C, D)."
STAMBPL1 activates TRAF2.
| 1
| 1

reach
"Especially STAMBPL1 could modulate the stability of TRAF2 protein and activate WNT/PI3K/NF-kb signalling pathway."
STAMBPL1 affects GRHL3
| 11
STAMBPL1 increases the amount of GRHL3.
| 8
STAMBPL1 increases the amount of GRHL3. 8 / 8
| 8

reach
"Furthermore, we discovered that STAMBPL1 regulates GRHL3 transcription by interacting with the transcription factor FOXO1."

reach
"Silencing of STAMBPL1 inhibited GRHL3 transcription in both HCC1806 and HCC1937 cells (Figure 4B, Figure 4—figure supplement 1B)."

reach
"Conversely, the overexpression of STAMBPL1 promoted GRHL3 transcription (Figure 4D, Figure 4—figure supplement 1D)."

reach
"STAMBPL1 mediates GRHL3 transcription by interacting with FOXO1."

reach
"Therefore, we aimed to investigate whether STAMBPL1 promotes GRHL3 transcription via FOXO1."

reach
"To investigate the mechanism by which STAMBPL1 promotes GRHL3 transcription through FOXO1, we conducted immunofluorescence and immunoprecipitation assays."

reach
"These findings suggest that STAMBPL1 promotes GRHL3 transcription by interacting with FOXO1."

reach
"Through RNA-seq analysis, we revealed that STAMBPL1 positively regulates the transcription of GRHL3."
STAMBPL1 activates GRHL3.
| 3
| 3

reach
"In summary, STAMBPL1 promoted TNBC angiogenesis by activating the GRHL3/HIF1α/VEGFA pathway via interacting with FOXO1 in a non-enzymatic manner."

reach
"Our study reveals for the first time that GRHL3 promotes transcriptional activity by binding to the promoter of the HIF1A gene.In this study, we utilized two TNBC cell lines, HCC1806 and HCC1937, along with human primary umbilical vein endothelial cells (HUVECs) and a nude mouse breast orthotopic transplantation tumor model to investigate the regulatory mechanism by which STAMBPL1 activates the GRHL3/HIF1α/VEGFA signaling pathway through its interaction with FOXO1, thereby promoting angiogenesis in TNBC."

reach
"These findings suggest that STAMBPL1 activates the HIF1α/VEGFA axis by upregulating GRHL3."
UBD affects STAMBPL1
| 3 7
| 2 7

sparser
"How JAMM/MPNDUBs cleave ubiquitin chains with K63-linkage specificity has been explained by the crystal structure of AMSH-LP bound to K63-linked di-ubiquitin: Two insertion loops orient the distal and proximal ubiquitin in order to place the isopeptide bond at lysine 63 in direct proximity to the active site [ xref ]."

sparser
"The crystal structure of the catalytic core domain of AMSH-LP bound to K63-linked diubiquitin has provided a molecular description of this specificity xref ."

reach
"(These observations are also true in the structure of AMSH-LP bound to diubiquitin.)"

sparser
"Only recently was Lys63-linkage specificity understood from the structural analysis of the DUB domain of AMSH-LP bound Lys63-linked diubiquitin. xref This structure reveals interactions between the enzyme and both the distal and proximal ubiquitins, but the basis for specificity arises from residues in the proximal site of the enzyme interacting with the proximal ubiquitin, despite a more extensive interaction with the distal ubiquitin (a significantly more accessible surface area is buried upon interaction with distal than proximal ubiquitin). xref Overall, AMSH adopts very similar interactions when modeled according to the ubiquitin-bound structure of AMSH-LP."

sparser
"However, this residue, in fact, the two-residue turn segment, Leu402-Phe403, needs to move apart relative to Thr316 to make room for the diubiquitin substrate to position itself correctly in the active site. (These observations are also true in the structure of AMSH-LP bound to diubiquitin.) It seems likely that the β-turn segment is dynamic, fluctuating between open and closed forms; the substrate binds to the open form, perhaps by conformational selection."

sparser
"The crystal structure of the catalytic domain of AMSH-LP bound to its substrate Lys63-linked diubiquitin (PBD ID: 2ZNV) reveals the flap adopting a closed position over the active site ( xref )."

reach
"Recent structure determination of the K63 linked diubiquitin bound to AMSH-LP revealed specific interactions between the proximal ubiquitin and the DUB catalytic core as well as an inserted motif."

sparser
"The structure of AMSH-LP bound to K63-linked diubiquitin demonstrates that the recognition of the proximal ubiquitin in diubiquitin determines AMSH-LP linkage selectivity xref ."

sparser
"Modeling of the catalytic domain of AMSH onto the structure of AMSH-LP bound to Lys63-linked diubiquitin revealed four residues within AMSH (Thr341, Phe343, Ser346, and Phe395) that could determine its specificity for Lys63-linked polyubiquitin chains by recognizing the tri-peptide sequence motif Gln62-Lys63-Glu64 within the proximal ubiquitin, which encompasses the acceptor Lys63 and its two immediate flanking residues."
STAMBPL1 binds UBD and Phe403. 1 / 1
| 1

reach
"X-ray crystal structures of the DUB domains of both AMSH-LP and Sst2 bound to diubiquitin shows Phe403 collapsed onto the Lys63 side chain of the proximal ubiquitin; however, the flap residues appear to retain their mobility even in presence of bound substrate or product (indicated by relatively weak electron density enveloping these residues and higher B-factors than internal residues)."
STAMBPL1 affects DUSP1
3 | 5 2
STAMBPL1 binds DUSP1.
3 | 2 2
3 | 2 2

sparser
"STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination."

reach
"STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination."

reach
"The study by Liu et al. (2022) found that STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination, further promoting breast cancer cell resistance to cisplatin."

sparser
"The study by xref found that STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination, further promoting breast cancer cell resistance to cisplatin."
STAMBPL1 activates DUSP1.
| 2
| 2

reach
"Our previous research revealed that STAMBPL1, a deubiquitinase, promotes cisplatin resistance in TNBC by stabilizing MKP1 (Liu et al., 2022)."

reach
"Our previous research revealed that the deubiquitinase STAMBPL1 enhances the stability of MKP-1, thereby promoting cisplatin resistance in breast cancer."
STAMBPL1 increases the amount of DUSP1.
| 1
STAMBPL1 increases the amount of DUSP1. 1 / 1
| 1

reach
"STAMBPL1 promotes breast cancer cell resistance to cisplatin partially by stabilizing MKP-1 expression."
TBMS-1 affects STAMBPL1
| 9
TBMS-1 decreases the amount of STAMBPL1.
| 5
TBMS-1 decreases the amount of STAMBPL1. 5 / 5
| 5

reach
"However, TBMS-1 significantly downregulated the expression of STAMBPL1, a DUB of c-FLIP, but had no effect on other DUBs (USP2, USP8, and USP9x) (Figure 4A)."

reach
"Additionally, TBMS-1 treatment downregulated c-FLIP and STAMBPL1 expression in HeLa and A549 cells; however, such effects were absent in normal EA.hy926 cells (Figure 4B)."

reach
"Interestingly, the results of this study showed that TBMS-1 only downregulates STAMBPL1 expression (Figure 4A,B)."

reach
"These findings were further confirmed in vivo using a xenograft model based on the observation that combined treatment with TBMS-1 and TRAIL decreased tumor volume and downregulated STAMBPL1 and c-FLIP expression levels."

reach
"Importantly, the combination of TBMS-1 and TRAIL therapy reduced the tumor volume and downregulated the expression levels of STAMBPL1 and c-FLIP in an in vivo xenograft model."
TBMS-1 inhibits STAMBPL1.
| 3
TBMS-1 inhibits STAMBPL1. 3 / 3
| 3

reach
"Particularly, pretreatment with CHX rapidly enhanced the degradation of STAMBPL1 by TBMS-1 (Figure 4F)."

reach
"Therefore, TBMS-1 downregulated STAMBPL1 at the post-translational regulation level."

reach
"Taken together, our findings indicated that TBMS-1 downregulates c-FLIP by downregulating STAMBPL1 deubiquitinating enzymes in various cancer cells."
TBMS-1 activates STAMBPL1.
| 1
TBMS-1 activates STAMBPL1. 1 / 1
| 1

reach
"Further mechanism studies have shown that TBMS-1 significantly increases sensitivity to TRAIL by downregulating the ubiquitinase STAMBPL1 (DUB) to disrupt c-FLIP expression."
STAMBPL1 affects mTORC1
| 9
STAMBPL1 activates mTORC1.
| 5
| 5

reach
"When leucine is sufficient, STAM-binding-protein-like 1 (STAMBPL) removes the ubiquitin chain on Sestrin2 and activates the mTORC1 signal [80]."

reach
"STAMBPL1 knockout significantly suppressed mTORC1 activity, anchorage-independent cell growth, and xenograft tumor growth ( Figures 5 F–5J and S8 C–S8J) in all three cell lines."

reach
"When overexpressed in cells, STAMBPL1 catalyzed the deubiquitination of Sestrin2 ( Figure 2 C) and promoted mTORC1 activation ( Figure 2 D)."

reach
"Accordingly, STAMBPL1(K405Rfs ∗ 21) showed higher interaction level with Sestrin2, even in the absence of amino acids or leucine ( Figures 6 B and S9 C), and activated mTORC1 signaling ( Figure 6 C)."

reach
"To further test if the catalytic activity of STAMBPL1 is required to modulate mTORC1 signaling, we mutated the water-activating Glu 292 (E292A) and the Zn 2+ -coordinating Asp 360 (D360A) residues in [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 inhibits mTORC1.
| 2
| 1

reach
"Correction of the STAMBPL1(K405Rfs ∗ 21) mutation in LS174T colon cancer cells inhibited mTORC1 activity and suppressed anchorage-independent cell growth ( Figures 6 F, 6G, and S9 D)."
STAMBPL1 bound to SESN2 inhibits mTORC1. 1 / 1
| 1

reach
"Inhibiting the interaction between STAMBPL1 and Sestrin2 thereby inactivating mTORC1 using a cell-permeable peptide could be a potential treatment approach for gastric and colorectal cancers [141]."
STAMBPL1 ubiquitinates mTORC1.
| 1
STAMBPL1 leads to the ubiquitination of mTORC1. 1 / 1
| 1

reach
"Interestingly, Wang et al. reported that gastric cancer and colorectal cancer downregulate an E3 ubiquitin ligase RING finger protein 167 (RNF167) and upregulate a deubiquitinase STAMBPL1 that can prevent the ubiquitination of Sestrin2, decrease Sestrin2-GATOR2 interaction and increase mTORC1 signaling [119]."
STAMBPL1 deubiquitinates mTORC1.
| 1
Mutated STAMBPL1 leads to the deubiquitination of mTORC1. 1 / 1
| 1

reach
"Consistent with the above results, endogenous K405Rfs ∗ 21 mutation of STAMBPL1 promoted the deubiquitination of Sestrin2 and activated mTORC1 signaling ( Figures 6 D and 6E)."
STAMBPL1 affects HIF1
| 9
STAMBPL1 activates HIF1.
| 7
STAMBPL1 activates HIF1. 7 / 7
| 7

reach
"In summary, STAMBPL1 promoted TNBC angiogenesis by activating the GRHL3/HIF1α/VEGFA pathway via interacting with FOXO1 in a non-enzymatic manner."

reach
"In this study, we discovered that STAMBPL1 promotes angiogenesis in TNBC by activating the HIF1α/VEGFA pathway independently of its enzymatic activity."

reach
"Under normoxic conditions, both STAMBPL1 and its enzymatically mutated variant (E292A) can upregulate the protein expression of HIF1α (Figure 1F)."

reach
"To elucidate how STAMBPL1 upregulates HIF1α, we examined the transcription levels of HIF1A when STAMBPL1 was knocking down under hypoxia."

reach
"These findings suggest that STAMBPL1 activates the HIF1α/VEGFA axis in TNBC cells, leading to enhanced abilities of HUVECs through a paracrine pathway."

reach
"These findings suggest that STAMBPL1 activates the HIF1α/VEGFA axis by upregulating GRHL3."

reach
"Our study reveals for the first time that GRHL3 promotes transcriptional activity by binding to the promoter of the HIF1A gene.In this study, we utilized two TNBC cell lines, HCC1806 and HCC1937, along with human primary umbilical vein endothelial cells (HUVECs) and a nude mouse breast orthotopic transplantation tumor model to investigate the regulatory mechanism by which STAMBPL1 activates the GRHL3/HIF1α/VEGFA signaling pathway through its interaction with FOXO1, thereby promoting angiogenesis in TNBC."
STAMBPL1 increases the amount of HIF1.
| 2
STAMBPL1 increases the amount of HIF1. 2 / 2
| 2

reach
"These findings suggest that STAMBPL1 enhances the expression of HIF1α in breast cancer cells through a non-DUB enzyme activity mechanism."

reach
"In this study, we discovered that, independent of its deubiquitinating enzyme activity, STAMBPL1 upregulates HIF1α expression in TNBC cells."
STAMBP affects STAMBPL1
2 | 2 5
2 | 2 4

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30)."

sparser
"Unlike the monomer of AMSH or AMSH-LP with inherent DUB activity, BRCC36 must form a complex with other subunits to specifically cleave K63-linked polyubiquitin chains."

sparser
"To quantify the interaction of the UbVs with STAMBP and STAMBPL1 and to validate the key residues responsible for the tight binding, we used isothermal titration calorimetry (ITC) to measure the interaction thermodynamics ( xref )."

sparser
"Hameed et al. developed a zinc chelating ABP that forms a tight complex with STAMBP and STAMBPL1 and inhibits the activity of the proteasomal DUB Rpn11 (PSMD14 in humans)."

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30) ."
| DOI

sparser
"Hence, it is possible that AMSH and AMSH-LP might be associated with a proteasome or other unknown large protein complex in the nucleus through their MPN domain."

sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
FOXO1 affects STAMBPL1
| 9

sparser
"The study also shows that STAMBPL1 interacts with FOXO1 to promote GRHL3 transcription, which in turn activates HIF1A."

sparser
"Because the authors find that STAMBPL1 and FOXO1 interact, they suggest that STAMBPL1 may promote the formation of an active transcriptional complex containing FOXO1, perhaps by facilitating the recruitment of transcriptional coactivators."

sparser
"The results of the GST pull-down assay indicated that the N-terminus (1–140 aa) of STAMBPL1 interacted with FOXO1 ( xref )."

sparser
"Since the interaction sites of STAMBPL1-FOXO1 have been mapped, a mutant disrupting the interaction would provide better insight into how STAMBPL1 promotes GRHL3 transcription by interacting with FOXO1."

sparser
"Additionally, we found that STAMBPL1 interacts with FOXO1 to facilitate the transcription of GRHL3/HIF1α/VEGFA."

sparser
"This interaction between STAMBPL1 and FOXO1 does not necessarily affect the binding of FOXO1 with DNA, including the GRHL3 promoter."

sparser
"Additionally, an immunoprecipitation assay revealed an interaction between the FOXO1 protein (250–596 aa) and STAMBPL1 ( xref )."

sparser
"They demonstrate that STAMBPL1 interacts with FOXO1, define the required domains in each protein, and illustrate that this interaction facilitates FOXO1 transcriptional factor activity, which then activates GRHL3/HIF1a/VEGFA signaling."

sparser
"Furthermore, it would be important to determine whether the STAMBPL1-FOXO1 interaction is essential for GRHL3 transcription."
STAMBPL1 affects SNAI1
| 2 6
STAMBPL1 binds SNAI1.
| 6

sparser
"Hence, we analysed 35 additional human tumour types for a STAMBPL1-SNAI1 co-signature (Fig.  xref )."

sparser
"Importantly, we found that 24 tumour types showed a positive correlation, out of which >4000 samples in 12 TCGA tumours displayed a significant STAMBPL1-SNAI1 co-expression profile (Fig.  xref )."

sparser
"A significant STAMBPL1-SNAI1 co-signature was observed across multiple tumour types."

sparser
"Altogether, these data highlight the prevalence of the identified STAMBPL1-SNAI1 association across an extensive number of tumours types."

sparser
"While our study does not address the mechanistic details on this regulation, we uncover a substantial STAMBPL1-SNAI1 link across multiple human tumours."

sparser
"Prevalence of a STAMBPL1-SNAI1 co-signature across a spectrum of tumour types."
STAMBPL1 inhibits SNAI1.
| 1
| 1

reach
"By downregulating the level of Ubiquitin using RNA interference-mediated knockdown of Ubb and Ubc, we found that knockdown of UbC that instructs tandem units of ubiquitin with nine repeats resulted in more efficient attenuation of both K48 and total and mono-Ub levels without affecting STAMBPL1 levels (Supplementary Fig. S3e) and that downregulation of UbC effectively hindered STAMBPL1-mediated Snail protein reduction (Fig. 4i)."
STAMBPL1 activates SNAI1.
| 1
| 1

reach
"Since STAMBPL1 has been shown to be an indirect activator of nuclear factor-κB) (NF-κB) signalling, and given that this pathway promotes Snail stabilisation through the synthesis of COP9 signalosome 2 protein (CSN2), we assessed if CSN2 is relevant for STAMBPL1 effect on Snail."
BIRC5 affects STAMBPL1
3 | 1 4
3 | 1 2

sparser
"However, although cepharanthine reduces STAMBPL1 expression and overexpression of STAMBPL1 inhibits cepharanthine-mediated downregulation of survivin, interaction between STAMBPL1 and survivin was not investigated."

reach
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."

sparser
"As shown in xref C, we detected the interaction between STAMBPL1 and survivin."
| 2

sparser
"Thus, these data reveal that STAMBPL1 directly interacts with survivin and c-FLIP, resulting in stabilization of proteins."

sparser
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
SNAI1 affects STAMBPL1
| 1 6
SNAI1 binds STAMBPL1.
| 6

sparser
"Hence, we analysed 35 additional human tumour types for a STAMBPL1-SNAI1 co-signature (Fig.  xref )."

sparser
"Importantly, we found that 24 tumour types showed a positive correlation, out of which >4000 samples in 12 TCGA tumours displayed a significant STAMBPL1-SNAI1 co-expression profile (Fig.  xref )."

sparser
"A significant STAMBPL1-SNAI1 co-signature was observed across multiple tumour types."

sparser
"Altogether, these data highlight the prevalence of the identified STAMBPL1-SNAI1 association across an extensive number of tumours types."

sparser
"While our study does not address the mechanistic details on this regulation, we uncover a substantial STAMBPL1-SNAI1 link across multiple human tumours."

sparser
"Prevalence of a STAMBPL1-SNAI1 co-signature across a spectrum of tumour types."
SNAI1 inhibits STAMBPL1.
| 1
| 1

reach
"By genetically depleting STAMBPL1 in these cells, we observed a primary effect on Snail protein levels (Fig. 4b)."
DUSP1 affects STAMBPL1
3 | 2 2
3 | 2 2

sparser
"STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination."

reach
"STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination."

reach
"The study by Liu et al. (2022) found that STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination, further promoting breast cancer cell resistance to cisplatin."

sparser
"The study by xref found that STAMBPL1 interacts with MKP-1 and stabilizes MKP-1 via deubiquitination, further promoting breast cancer cell resistance to cisplatin."
| 6

reach
"STAMBPL1 activates the GRHL3/HIF1A/VEGFA axis through interaction with FOXO1 to promote angiogenesis in triple-negative breast cancer."

reach
"In summary, STAMBPL1 promoted TNBC angiogenesis by activating the GRHL3/HIF1α/VEGFA pathway via interacting with FOXO1 in a non-enzymatic manner."

reach
"Therefore, we investigated whether STAMBPL1 promotes tumor angiogenesis."

reach
"In this study, we discovered that STAMBPL1 promotes angiogenesis in TNBC by activating the HIF1α/VEGFA pathway independently of its enzymatic activity."

reach
"STAMBPL1 in TNBC cells enhances the activity of HUVECs and promotes TNBC angiogenesis."

reach
"Knocking down STAMBPL1 inhibited the growth of HCC1806 xenografts in mice (Figure 3G–I) and decreased the number of microvessels in tumor tissue (Figure 3J, K), indicating that STAMBPL1 promoted tumor angiogenesis."
STAMBPL1 affects XIAP
| 6
STAMBPL1 inhibits XIAP.
| 3
| 3

reach
"These data suggest that STAMBPL1 loss diverts the XIAP degradation from proteasome pathway to lysosome pathway, and drastically enhances XIAP degradation.In this study, we reported evidence that silen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"STAMBPL1 depletion was found to induce apoptosis by promoting the degradation of the protein XIAP, suggesting that targeting STAMBPL1 might offer a promising therapeutic strategy for prostate cancer [23]."

reach
"Of interest, XIAP protein was diminished by STAMBPL1 RNAi."
STAMBPL1 increases the amount of XIAP.
| 1
STAMBPL1 increases the amount of XIAP. 1 / 1
| 1

reach
"Albeit the sharp drop seen in XIAP protein level in STAMBPL1 depleted cells, we did not observe any change in XIAP mRNA level ( Fig. 6 A), indicating that regulation of XIAP expression by STAMBPL1 is [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 deubiquitinates XIAP.
| 1
STAMBPL1 leads to the deubiquitination of XIAP. 1 / 1
| 1

reach
"We observed that STAMBPL1 depletion modulates XIAP ubiquitination status, which diverts the XIAP degradation from proteasome pathway to lysosome pathway."
STAMBPL1 activates XIAP.
| 1
STAMBPL1 activates XIAP. 1 / 1
| 1

reach
"We demonstrated that STAMBPL1 RNAi depletion induced apoptosis in PC3 and DU145 cells by promoting XIAP lysosomal degradation."
STAMBPL1 affects HIF1A
| 6
STAMBPL1 increases the amount of HIF1A.
| 5
STAMBPL1 increases the amount of HIF1A. 5 / 5
| 5

reach
"We demonstrated that STAMBPL1 increased HIF1A transcription in a non-enzymatic manner, thereby activating the HIF1alpha/VEGFA signaling pathway to facilitate triple-negative breast cancer angiogenesis."

reach
"STAMBPL1 promotes HIF1A transcription and activates the HIF1alpha/VEGFA axis."

reach
"Data showed that STAMBPL1 overexpression increased the transcription of HIF1A (Figure 4E, Figure 4—figure supplement 1E) and VEGFA (Figure 2E, G)."

reach
"These findings suggested that STAMBPL1 activated the HIF1α/VEGFA axis through enhancing the transcription of HIF1A."

reach
"By silencing the STAMBPL1 gene in HCC1806 cells subjected to 10 hr of hypoxia, we performed RNA-seq analysis to investigate the mechanism by which STAMBPL1 promotes HIF1A transcription."
STAMBPL1 activates HIF1A.
| 1
| 1

reach
"STAMBPL1 upregulates HIF1alpha in a non-enzymatic manner in TNBC cells."
AXL affects STAMBPL1
| 6
AXL binds STAMBPL1.
| 5
| 5

sparser
"DUBs employ diverse mechanisms and modes of interaction with their substrates to mediate the removal of mono‐ubiquitin or poly‐ubiquitin chains from the target protein. [ xref ] To ascertain the direct interaction between STAMBPL1 and AXL, Immunoprecipitation (IP) and in vitro pull‐down assays were performed."

sparser
"Based on their interaction, we next identified the critical region within STAMBPL1 that binds to AXL."

sparser
"Our results showed truncated mutant lacking USP8_dimer domain exhibited much lower binding affinity with AXL comparing with STAMBPL1‐WT, STAMBPL1‐ΔUJ_linker and STAMBPL1‐ΔJAB, indicating that STAMBPL1 interacts with AXL largely through USP8_dimer domain (Figure  xref )."

sparser
"First, our data showed that the interaction between STAMBPL1 and AXL proteins in KIRC cells was not affected upon GAS6 stimulation (Figure  xref )."

sparser
"Thus, STAMBPL1 binding to AXL will sterically block TRIM21 from recognizing the AXL and inhibit the subsequent ubiquitination of AXL."
AXL ubiquitinates STAMBPL1.
| 1
AXL ubiquitinates STAMBPL1. 1 / 1
| 1

sparser
"Of note, STAMBPL1 silencing failed to downregulate AXL protein level in TRIM21 KD 786‐O cells, implying that STAMBPL1 KD‐induced AXL ubiquitination and degradation was dependent on TRIM21 (Figure  xref )."
Ubiquitin affects K63
| 5
| 4

sparser
"The structure was determined by molecular replacement using a model of Sst2–K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref )) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry (Table xref )."

sparser
"The overall three-dimensional fold of Sst2 Δ220 –K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 Å of Cα atoms over 266 residues from both Sst2 and K63-Ub 2 ."

sparser
"Figure xref d shows the superposition of Sst2 Δ220 –K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref ))."

sparser
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2 ."

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
| 5
STAMBPL1 activates cell growth.
| 4

reach
"Knockdown of STAMBPL1 inhibited cell growth and metastasis of CRC through inactivation of the NF-κB pathway."

reach
"Our findings indicated that STAMBPL1 overexpression enhanced breast cancer cell growth in vivo."

reach
"STAMBPL1 knockout significantly suppressed mTORC1 activity, anchorage-independent cell growth, and xenograft tumor growth ( Figures 5 F–5J and S8 C–S8J) in all three cell lines."

reach
"We also showed that knockout of STAMBPL1 in HEK293T cells activated the catabolic autophagy pathway ( Figures S7 A and S7B), reduced the cell size ( Figure S7 C), and suppressed anchorage-independent [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 inhibits cell growth.
| 1

reach
"Correction of the STAMBPL1(K405Rfs ∗ 21) mutation in LS174T colon cancer cells inhibited mTORC1 activity and suppressed anchorage-independent cell growth ( Figures 6 F, 6G, and S9 D)."
STAMBPL1 affects STAM
| 5
| 3

sparser
"We observed a significant enhancement of IL-2-mediated c- myc induction in AMSH-LP-transfected cells as well as AMSH-transfected cells, although AMSH-LP did not associate physically with either STAM [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We addressed whether the SBM-like motif of AMSH-LP could bind STAM1."

sparser
"These results indicate that, unlike AMSH, AMSH-LP cannot bind to the STAMs."
| 1

sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
| 1

sparser
"Another STAM family protein, STAM2, did not bind to AMSH-LP either, although AMSH strongly binds to STAM2 (data not shown)."
STAMBPL1 affects K63-Ub 2
| 5
STAMBPL1 binds K63-Ub 2. 4 / 4
| 4

reach
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2."

reach
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."

reach
"Figure XREF_FIG d shows the superposition of Sst2 Delta220 -K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV)."

reach
"The structure was determined by molecular replacement using a model of Sst2-K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry."
STAMBPL1 binds K63-Ub 2 and DUB domain. 1 / 1
| 1

reach
"The overall three-dimensional fold of Sst2 Delta220 -K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 A of Calpha atoms over 266 residues from both Sst2 and K63-Ub 2."
STAMBPL1 affects K63
| 5
| 4

sparser
"The structure was determined by molecular replacement using a model of Sst2–K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref )) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry (Table xref )."

sparser
"The overall three-dimensional fold of Sst2 Δ220 –K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 Å of Cα atoms over 266 residues from both Sst2 and K63-Ub 2 ."

sparser
"Figure xref d shows the superposition of Sst2 Δ220 –K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref ))."

sparser
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2 ."

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
STAMBPL1 affects HYCC1
| 5
| 5

reach
"These findings highlight the diverse roles that deubiquitinases play in HCC, either by promoting or suppressing the disease, and suggest that they could be potential targets for therapeutic intervention in HCC.In the study, we identified one oncogene gene (STAMBPL1) which promote the proliferation and metastasis of HCC."

reach
"Through the analysis of TCGA expression symbol and clinicopathological information, it was found that the expression of STAMBPL1 increased with the variation of tumor stage (Fig. 1K) and grade (Fig. 1M) in HCC."

reach
"Additionally, the EDU staining also demonstrated that suppression of STAMBPL1 expression could reduce the proportion of proliferative HCC cells (Fig. 3I, J)."

reach
"Additionally, the EDU staining also demonstrated that overexpression of STAMBPL1 expression could enhance the proportion of proliferative HCC cells (Fig. 4G)."

reach
"Functionally, STAMBPL1 significantly promoted HCC cells proliferation and metastasis, and it interacts with TRAF2 and stabilize it via the deubiquitination at the K63 residue."
STAMBPL1 affects FAS
| 5
| 5

sparser
"Seven of the nine fusions frequently detected in patients with HCC included: MAN2A1–FER (100%), SLC45A2–AMACR (62.3%), ZMPSTE24–ZMYM4 (62.3%), PTEN–NOLC1 (57.4%), CCNH–C5orf30 (55.7%), STAMBPL1FAS (26.2%), and PCMTD1–SNTG1 (16.4%)."

sparser
"STAMBPL1-FAS fusion isoform was identified in sample 1 M (metastasis cancer) and 2 M (the metastasis cancer): XM_011539985 from STAMBPL1 and XM_011539766.2 from FAS (Fig.  xref )."

sparser
"However, subsequent analysis using Taqman qPCR showed that the STAMBPL1-FAS fusion isoform can be found in all 6 cancer samples (Supplementary Fig.  xref ), implying a wider distribution of this gene fusion in colon cancers."

sparser
"The relatively low-frequency fusion transcripts were STAMBPL1FAS (26.2%, 16/61), PCMTD1–SNTG1 (16.4%, 10/61), and ZNF124–SMYD3 (16.4%, 10/61)."

sparser
"The Fisher exact test showed that seven fusion genes— MAN2A1–FER ( P  = 1.4 × 10 −19 ), CCNH–C5orf30 ( P  = 1.1 × 10 −5 ), SLC45A2–AMACR ( P  = 2.8 × 10 −9 ), ZMPSTE24–ZMYM4 ( P  = 9.3 × 10 −6 ), PTEN–NOLC1 ( P  = 1.8 × 10 −6 ), PCMTD1–SNTG1 ( P  = 0.006), and STAMBPL1FAS ( P  = 0.01)—had significantly greater frequencies of expression in the serum samples of patients with HCC, suggesting that the detection of these fusion transcripts in the serum samples of patients with HCC likely predicts the risk for HCC."
STAMBPL1 affects AXL
| 5
| 5

sparser
"DUBs employ diverse mechanisms and modes of interaction with their substrates to mediate the removal of mono‐ubiquitin or poly‐ubiquitin chains from the target protein. [ xref ] To ascertain the direct interaction between STAMBPL1 and AXL, Immunoprecipitation (IP) and in vitro pull‐down assays were performed."

sparser
"Based on their interaction, we next identified the critical region within STAMBPL1 that binds to AXL."

sparser
"Our results showed truncated mutant lacking USP8_dimer domain exhibited much lower binding affinity with AXL comparing with STAMBPL1‐WT, STAMBPL1‐ΔUJ_linker and STAMBPL1‐ΔJAB, indicating that STAMBPL1 interacts with AXL largely through USP8_dimer domain (Figure  xref )."

sparser
"First, our data showed that the interaction between STAMBPL1 and AXL proteins in KIRC cells was not affected upon GAS6 stimulation (Figure  xref )."

sparser
"Thus, STAMBPL1 binding to AXL will sterically block TRIM21 from recognizing the AXL and inhibit the subsequent ubiquitination of AXL."
STAM affects STAMBPL1
| 5
| 3

sparser
"We observed a significant enhancement of IL-2-mediated c- myc induction in AMSH-LP-transfected cells as well as AMSH-transfected cells, although AMSH-LP did not associate physically with either STAM [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We addressed whether the SBM-like motif of AMSH-LP could bind STAM1."

sparser
"These results indicate that, unlike AMSH, AMSH-LP cannot bind to the STAMs."
| 1

sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
| 1

sparser
"Another STAM family protein, STAM2, did not bind to AMSH-LP either, although AMSH strongly binds to STAM2 (data not shown)."
K63-Ub 2 affects STAMBPL1
| 5
STAMBPL1 binds K63-Ub 2. 4 / 4
| 4

reach
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2."

reach
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."

reach
"Figure XREF_FIG d shows the superposition of Sst2 Delta220 -K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV)."

reach
"The structure was determined by molecular replacement using a model of Sst2-K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry."
STAMBPL1 binds K63-Ub 2 and DUB domain. 1 / 1
| 1

reach
"The overall three-dimensional fold of Sst2 Delta220 -K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 A of Calpha atoms over 266 residues from both Sst2 and K63-Ub 2."
K63 affects Ubiquitin
| 5
| 4

sparser
"The structure was determined by molecular replacement using a model of Sst2–K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref )) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry (Table xref )."

sparser
"The overall three-dimensional fold of Sst2 Δ220 –K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 Å of Cα atoms over 266 residues from both Sst2 and K63-Ub 2 ."

sparser
"Figure xref d shows the superposition of Sst2 Δ220 –K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref ))."

sparser
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2 ."

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
FAS affects STAMBPL1
| 5
| 5

sparser
"Seven of the nine fusions frequently detected in patients with HCC included: MAN2A1–FER (100%), SLC45A2–AMACR (62.3%), ZMPSTE24–ZMYM4 (62.3%), PTEN–NOLC1 (57.4%), CCNH–C5orf30 (55.7%), STAMBPL1FAS (26.2%), and PCMTD1–SNTG1 (16.4%)."

sparser
"STAMBPL1-FAS fusion isoform was identified in sample 1 M (metastasis cancer) and 2 M (the metastasis cancer): XM_011539985 from STAMBPL1 and XM_011539766.2 from FAS (Fig.  xref )."

sparser
"However, subsequent analysis using Taqman qPCR showed that the STAMBPL1-FAS fusion isoform can be found in all 6 cancer samples (Supplementary Fig.  xref ), implying a wider distribution of this gene fusion in colon cancers."

sparser
"The relatively low-frequency fusion transcripts were STAMBPL1FAS (26.2%, 16/61), PCMTD1–SNTG1 (16.4%, 10/61), and ZNF124–SMYD3 (16.4%, 10/61)."

sparser
"The Fisher exact test showed that seven fusion genes— MAN2A1–FER ( P  = 1.4 × 10 −19 ), CCNH–C5orf30 ( P  = 1.1 × 10 −5 ), SLC45A2–AMACR ( P  = 2.8 × 10 −9 ), ZMPSTE24–ZMYM4 ( P  = 9.3 × 10 −6 ), PTEN–NOLC1 ( P  = 1.8 × 10 −6 ), PCMTD1–SNTG1 ( P  = 0.006), and STAMBPL1FAS ( P  = 0.01)—had significantly greater frequencies of expression in the serum samples of patients with HCC, suggesting that the detection of these fusion transcripts in the serum samples of patients with HCC likely predicts the risk for HCC."
| 4
STAMBPL1 inhibits cell death.
| 2

reach
"Taken together, these data indicate that inhibition of STAMBPL1 in prostate cancer cells triggers caspase-dependent apoptosis.Considering that caspase 8 is the predominant initiator caspase in the cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"To elucidate the machinery of STAMBPL1 depletion-induced cell death, we tested necroptosis inhibitor necrostatin-1 (Nec-1), autophagy inhibitor chloroquine (CQ) and caspase 3 inhibitor Z-DEVD-FMK for [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 activates cell death.
| 2
| 2

reach
"Accordingly, overexpression of XIAP in STAMBPL1-silenced cells rescued cell death, supporting the notion that decreasing XIAP is the major mechanism underlying the cell death caused by STAMBPL1 downre[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Necroptosis inhibitor Nec-1 and autophagy inhibitor CQ did not efficiently suppress cell death induced by STAMBPL1 targeting; whereas, addition of a caspase 3 inhibitor, Z-DEVD-FMK, to cells markedly [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Functionally, STAMBPL1 notably enhances CCA cell proliferation and metastasis while impeding ferroptosis."

reach
"Functionally, STAMBPL1 significantly promoted HCC cells proliferation and metastasis, and it interacts with TRAF2 and stabilize it via the deubiquitination at the K63 residue."

reach
"These findings highlight the diverse roles that deubiquitinases play in HCC, either by promoting or suppressing the disease, and suggest that they could be potential targets for therapeutic intervention in HCC.In the study, we identified one oncogene gene (STAMBPL1) which promote the proliferation and metastasis of HCC."

reach
"Knockdown of STAMBPL1 inhibited cell growth and metastasis of CRC through inactivation of the NF-κB pathway."

reach
"Knockdown of STAMBPL1 reduced cell viability of CRC and suppressed the proliferation, invasion, and migration."

reach
"Cell invasion showed that the number of cells in A549 and H1299 of the STAMBPL1 SI group was lower than that of A549 STAMBPL1 NC and H1299 STAMBPL1 NC group, suggesting that STAMBPL1 promoted the cell invasion ability of LUAD (Fig. 2D, p<0.001, p<0.01)."

reach
"STAMBPL1 promotes cell migration and invasion in LUAD."

reach
"Furthermore, knockdown of STAMBPL1 by shSTAMBPL1 resulted in STAMBPL1 downregulation, which in turn decreased the cell proliferation of AGS gastric cancer cells, increasing cell apoptosis, cell invasion and migration in vitro."
STAMBPL1 affects NFkappaB
| 3
| 2

reach
"These data ruled out the dependence of cell death on caspase 8 activation, indicating cell-extrinsic pathway is not a mechanism underlying the STAMBPL1 depletion induced apoptosis.It was reported that[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Silencing of STAMBPL1 with siRNA inhibits Tax activation of both the canonical and noncanonical NF-kappaB signaling pathways."
| PMC
Modified STAMBPL1 activates NFkappaB. 1 / 1
| 1

reach
"Overexpression of wild-type STAMBPL1, but not a catalytically inactive mutant, enhances Tax mediated NF-kappaB activation and the induction of NF-kappaB target genes."
| PMC
STAMBPL1 affects DHRS2
| 4
STAMBPL1 increases the amount of DHRS2.
| 3
STAMBPL1 increases the amount of DHRS2. 3 / 3
| 3

reach
"STAMBPL1 promotes NSCLC progression by regulating DHRS2 expression."

reach
"STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression."

reach
"Besides, we found that the expression of DHRS2 in the primary tumor was lower than in normal samples in the TCGA-LUAD database (Fig. 3E), while the expression of STAMBPL1 in the primary tumor was higher than in normal samples (Fig. 3F), so we carried out the hypothesis that STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression."
STAMBPL1 decreases the amount of DHRS2.
| 1
STAMBPL1 decreases the amount of DHRS2. 1 / 1
| 1

reach
"Combined with microarray sequencing, qPCR, and WB results, we proved that knockdown of STAMBPL1 in LUAD A549 and H1299 cell lines could up-regulate DHRS2 gene expression."
K63 affects STAMBPL1, and Ubiquitin
| 4
| 4

sparser
"The structure was determined by molecular replacement using a model of Sst2–K63-Ub 2 generated by superimposition of Sst2 cat with the structure of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref )) and refined yielding satisfactory crystallographic, free R factors and good stereochemistry (Table xref )."

sparser
"The overall three-dimensional fold of Sst2 Δ220 –K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 Å of Cα atoms over 266 residues from both Sst2 and K63-Ub 2 ."

sparser
"Figure xref d shows the superposition of Sst2 Δ220 –K63-Ub 2 and AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV( xref ))."

sparser
"Proximal ubiquitin binding is similar to that observed in the crystal structure of AMSH-LP bound to K63-Ub 2 ."
Honokiol affects STAMBPL1
| 4
| 4

eidos
"Honokiol decreased STAMBPL1 expression , resulting in degradation of survivin and c-FLIP protein ."

eidos
"Therefore , we tested alteration of various DUBs expression and discovered that honokiol only decreased STAMBPL1 which is classified as JAMM family ( Figure 5B ) ."

eidos
"In our study , the honokiol inhibited STAMBPL1 expression ( Figure 5B ) ."

eidos
"Honokiol induced STAMBPL1 downregulation in tested cancer cells ( ACHN , A498 , A549 , and Hela cells ) ( Figure 6E ) ."
TP53 affects STAMBPL1
| 2 1
TP53 activates STAMBPL1.
| 1 1
Mutated TP53 activates STAMBPL1. 1 / 1
| 1

reach
"Thus, the mechanism by which mutant p53 might activate STAMBPL1 is therefore likely through its interaction with these TFs, which possess responsive to mutant p53."
TP53 activates STAMBPL1. 1 / 1
| 1

sparser
"Thus, the mechanism by which mutant p53 might activate STAMBPL1 is therefore likely through its interaction with these TFs, which possess responsive to mutant p53. xref – xref Although future studies are needed to delineate whether other DUBs are involved in mutant p53-induced EMT or to other mutant p53-mediated oncogenic pro-survival benefits (ref. xref , xref ), our findings suggest an involvement of mutant p53 in driving the expression of STAMBPL1 as a novel pro-EMT GOF, providing further rationale to investigate DUB expression by other oncoproteins."
TP53 increases the amount of STAMBPL1.
| 1
TP53 increases the amount of STAMBPL1. 1 / 1
| 1

reach
"While ChIP assay revealed p53 enrichment on MDM2 and CDKN1A promoters in p53 -expressing cells (up to ~4% and ~10% of input), no significant (>1%) occupancy on any of the six predicted was detected for either WT or p53 (Supplementary Fig. S5c), indicating that the expression of STAMBPL1 gene is not directly regulated by p53 ."
TNFSF10 affects STAMBPL1
| 3
TNFSF10 decreases the amount of STAMBPL1.
| 2
TNFSF10 decreases the amount of STAMBPL1. 2 / 2
| 2

reach
"These findings were further confirmed in vivo using a xenograft model based on the observation that combined treatment with TBMS-1 and TRAIL decreased tumor volume and downregulated STAMBPL1 and c-FLIP expression levels."

reach
"Importantly, the combination of TBMS-1 and TRAIL therapy reduced the tumor volume and downregulated the expression levels of STAMBPL1 and c-FLIP in an in vivo xenograft model."
TNFSF10 inhibits STAMBPL1.
| 1
| 1

reach
"Further mechanism studies have shown that TBMS-1 significantly increases sensitivity to TRAIL by downregulating the ubiquitinase STAMBPL1 (DUB) to disrupt c-FLIP expression."
STAMBPL1 affects VEGFA
| 2 1
STAMBPL1 increases the amount of VEGFA. 3 / 3
| 2 1

sparser
"For Fig.7G-H, our experimental objective was to determine whether the activation of HIF1A / VEGFA transcription by STAMBPL1 via FOXO1."

reach
"Data showed that STAMBPL1 overexpression increased the transcription of HIF1A (Figure 4E, Figure 4—figure supplement 1E) and VEGFA (Figure 2E, G)."

reach
"The ability of STAMBPL1 to induce VEGFA transcription was blocked by HIF1α knockdown (Figure 2D–G)."
STAMBPL1 affects Tax
| 3
STAMBPL1 activates Tax.
| 2
STAMBPL1 activates Tax. 2 / 2
| 2

reach
"Furthermore, overexpression of STAMBPL1 enhances the stability of Tax by blocking its proteasomal degradation, whereas siRNA silencing of STAMBPL1 promotes Tax degradation."
| PMC

reach
"Silencing of STAMBPL1 with siRNA inhibits Tax activation of both the canonical and noncanonical NF-kappaB signaling pathways."
| PMC
STAMBPL1 deubiquitinates Tax.
| 1
STAMBPL1 leads to the deubiquitination of Tax. 1 / 1
| 1

reach
"Importantly, STAMBPL1 does not induce Tax de-ubiquitination but protects Tax from degradation."
STAMBPL1 affects GST
| 3
| 3

sparser
"The GSTSTAMBPL1 or GST‐TRIM21 fusion proteins were produced in Escherichia coli (BL21) cells and purified using a GST‐Tag Protein Purification Kit (Sangon Biotech, Shanghai, China)."

sparser
"Indeed, endogenous binding between STAMBPL1 and AXL was observed in multiple KIRC cell lines (786‐O, 769‐P and Caki‐1), and the glutathione S‐transferase (GST) pull‐down experiments showed that AXL interacted with purified GSTSTAMBPL1, but not GST alone ( Figure xref ; Figure xref , Supporting Information)."

sparser
"Prokaryotic plasmids encoding GSTSTAMBPL1 or GST‐TRIM21 fusion protein were constructed in pGEX‐4T. The PD‐L1 wild‐type or mutant promoter sequence was constructed into pGL3‐basic firefly luciferase reporter vector."
STAMBPL1 affects Clathrin
| 2 1

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30)."

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30) ."
| DOI

sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
GST affects STAMBPL1
| 3
| 3

sparser
"The GSTSTAMBPL1 or GST‐TRIM21 fusion proteins were produced in Escherichia coli (BL21) cells and purified using a GST‐Tag Protein Purification Kit (Sangon Biotech, Shanghai, China)."

sparser
"Indeed, endogenous binding between STAMBPL1 and AXL was observed in multiple KIRC cell lines (786‐O, 769‐P and Caki‐1), and the glutathione S‐transferase (GST) pull‐down experiments showed that AXL interacted with purified GSTSTAMBPL1, but not GST alone ( Figure xref ; Figure xref , Supporting Information)."

sparser
"Prokaryotic plasmids encoding GSTSTAMBPL1 or GST‐TRIM21 fusion protein were constructed in pGEX‐4T. The PD‐L1 wild‐type or mutant promoter sequence was constructed into pGL3‐basic firefly luciferase reporter vector."
Clathrin affects STAMBPL1
| 2 1

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30)."

reach
"Both STAMBP and STAMBPL1 localize to early endosomes by binding to clathrin (31); however, STAMBPL1 fails to bind to STAM due to residue substitutions in the SBM-like motif, suggesting it may function in different signaling pathway from that of STAMBP (30) ."
| DOI

sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."

reach
"Especially STAMBPL1 could modulate the stability of TRAF2 protein and activate WNT/PI3K/NF-kb signalling pathway."

reach
"Our study reveals for the first time that GRHL3 promotes transcriptional activity by binding to the promoter of the HIF1A gene.In this study, we utilized two TNBC cell lines, HCC1806 and HCC1937, along with human primary umbilical vein endothelial cells (HUVECs) and a nude mouse breast orthotopic transplantation tumor model to investigate the regulatory mechanism by which STAMBPL1 activates the GRHL3/HIF1α/VEGFA signaling pathway through its interaction with FOXO1, thereby promoting angiogenesis in TNBC."
| 2

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"STAMBPL1 promotes cell migration and invasion in LUAD."

reach
"Cell migration showed that the number of A549 and H1299 cells in the STAMBPL1 SI group was lower than that in A549 STAMBPL1 NC and H1299 STAMBPL1 NC group, indicating that STAMBPL1 promoted cell migration of LUAD (Fig. 2C, p<0.001, p<0.001)."
STAMBPL1 affects TNFSF10
| 2
| 2

reach
"Conversely, STAMBPL1 knockdown enhanced sensitivity to TRAIL (Figure 4H)."

reach
"Moreover, STAMBPL1 knockdown markedly increased sensitivity to TRAIL by destabilizing c-FLIP."
STAMBPL1 affects TNBC
| 2
STAMBPL1 activates TNBC. 2 / 2
| 2

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"In summary, STAMBPL1 promoted TNBC angiogenesis by activating the GRHL3/HIF1α/VEGFA pathway via interacting with FOXO1 in a non-enzymatic manner."

reach
"In this study, we discovered that STAMBPL1 promotes angiogenesis in TNBC by activating the HIF1α/VEGFA pathway independently of its enzymatic activity."
STAMBPL1 affects TGFB
| 2
STAMBPL1 activates TGFB. 2 / 2
| 2

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"Finally, AMSH-LP and UCHL5 promote TGF-beta responses through their interaction with inhibitory I-SMADs [XREF_BIBR, XREF_BIBR]."

reach
"One further link between this pathway and endosomes concerns the parallel findings that the endosomal DUBs AMSH and AMSH-LP positively regulate the related bone morphogenic protein (BMP) and TGFβ sign[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 affects STAM2
| 2
| 1

sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
| 1

sparser
"Another STAM family protein, STAM2, did not bind to AMSH-LP either, although AMSH strongly binds to STAM2 (data not shown)."
STAMBPL1 affects SMAD7
| 1 1
STAMBPL1 inhibits SMAD7.
| 1
| 1

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"Similarly, AMSH-LP has been reported to sequester SMAD7, thereby exerting a positive impact on the TGFbeta pathway (XREF_FIG C) XREF_BIBR."
STAMBPL1 binds SMAD7.
| 1
| 1

sparser
"Previous studies reported that although they did not investigate the DUB activity of STAMBPL1, STAMBPL1 interact with Smad2 and Smad7, followed by induction of TGF-β-mediated transcriptional activity [ xref ]."
STAMBPL1 affects NFE2L2
| 1 1
STAMBPL1 binds NFE2L2.
| 1

sparser
"Particularly, sites 251-436 of STAMBPL1 interact with sites 228-605 of NRF2, facilitating DUB activity and eliminating ubiquitin molecules attached to NRF2, thus protecting it from proteasome-mediated degradation."
STAMBPL1 activates NFE2L2.
| 1
| 1

reach
"This study firstly reveals that STAMBPL1 promotes cholangiocarcinoma progression by upregulating NRF2, indicating that targeting the STAMBPL1/NRF2 axis is a novel therapeutic strategy."
STAMBPL1 affects JAG1
| 2
STAMBPL1 inhibits JAG1.
| 1
| 1

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"Furthermore, knockdown of STAMBPL1 by shSTAMBPL1 resulted in STAMBPL1 downregulation, which in turn decreased the cell proliferation of AGS gastric cancer cells, increasing cell apoptosis, cell invasion and migration in vitro."
STAMBPL1 activates JAG1.
| 1
STAMBPL1 activates JAG1. 1 / 1
| 1

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"These results suggested that AGS cell proliferation was suppressed by STAMBPL1 knockdown."
STAMBPL1 affects EGFR
| 2
STAMBPL1 activates EGFR. 2 / 2
| 2

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"STAMBPL1 augments RNA efficient splicing of EGFR to avoid intron retention by activating cleavage of the K63-linked ubiquitin chain on the target of EGR1 protein 1 (TOE1)."

reach
"Previous studies suggested that STAMBPL1 enhances the trafficking of EGFR to the cell surface by removing Lys 63-linked polyubiquitin chains [ 5 , 7 , 8 ]."
| 1 1
| 1 1

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"Knockdown of STAMBPL1 reduced cell viability of CRC and suppressed the proliferation, invasion, and migration."

eidos
"In addition , STAMBPL1 deubiquitinylates the anti-apoptotic protein Survivin and thus ameliorates cell survival ."

reach
"In addition, the results from the colony formation experiment demonstrated that the downregulation of STAMBPL1 suppressed the proliferation of hepatocellular carcinoma cells (Fig. 3H)."

reach
"STAMBPL1, transcriptionally regulated by SREBP1, promotes malignant behaviors of hepatocellular carcinoma cells via Wnt/β-catenin signaling pathway."
STAMBPL1 affects CRC
| 2
STAMBPL1 activates CRC. 2 / 2
| 2

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"Knockdown of STAMBPL1 reduced cell viability of CRC and suppressed the proliferation, invasion, and migration."

reach
"Knockdown of STAMBPL1 inhibited cell growth and metastasis of CRC through inactivation of the NF-κB pathway."
STAM2 affects STAMBPL1
| 2
| 1

sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
| 1

sparser
"Another STAM family protein, STAM2, did not bind to AMSH-LP either, although AMSH strongly binds to STAM2 (data not shown)."

reach
"Thus, H. pylori infection causes excessive OS and produces redundant ROS, which in turn downregulates the STAMBPL1 and contributes to the survival of GBC cells."

reach
"Recent studies have shown that excessive ROS generated in H. pylori infection induces direct degradation of STAMBPL1 by cullin 1-RING ubiquitin ligase and the 26S proteasome, while STAMBPL1 can lead to cell apoptosis by reducing the anti-apoptotic protein survivin (Chaithongyot and Naumann, 2022)."
STAMBPL1 is modified
| 2
STAMBPL1 is methylated. 2 / 2
| 2

sparser
"We found that dysregulation of miR-29C, miR-34A, miR-98, and miR-215; and methylation of ANKRD1, ARID5B, CDCA2, PIF1, STAMBPL1, TROAP, ZNF165 , and HIST1H2AJ in HeLa cells could result in cell proliferation and anti-apoptosis through NFκB, TGF-β, and PI3K pathways."

sparser
"It has been reported that NUF2, TROAP, STAMBPL1 , and ARID5B can be methylated in cancers. xref Furthermore, we used the DNA methylation profile of human cervical cancer and ESCs to validate our findings."
Neoplasm Invasiveness decreases the amount of STAMBPL1.
| 1
Neoplasm Invasiveness decreases the amount of STAMBPL1. 1 / 1
| 1

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"The transwell assays were used to evaluate the influence on invasion and metastasis abilities of HCC cells by reducing the expression of STAMBPL1."

reach
"The transwell assays and cell scratch assay both validated the influence of STAMBPL1 on migration and invasion ability of HCCLM3 and Hep3B cells, The migration and invasion ability of HCCLM3 and Hep3B cells was enhanced in the STAMBPL1-overexpressed group (Fig. 4I–J)."
NPRL2 affects STAMBPL1
| 2
| 2

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"Knockout of GATOR1 component DEPDC5 or NPRL2 in WT or STAMBPL1-null LS174T cells ( Figures S8 K and S8L) restored anchorage-independent cell growth and xenograft tumor growth in STAMBPL1-deficient cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Knockout of GATOR1 component DEPDC5 or NPRL2 restored mTORC1 activity and promoted anchorage-independent cell growth in STAMBPL1(K405Rfs ∗ 21) mutation-corrected LS174T cells ( Figures 6 G and S9 E–S9[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
NFkappaB affects STAMBPL1
| 2
NFkappaB inhibits STAMBPL1.
| 1
| 1

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"Overexpression of wild-type STAMBPL1, but not a catalytically inactive mutant, enhanced Tax mediated NF-kappaB activation, whereas silencing of STAMBPL1 with siRNA impaired Tax activation of both the canonical and noncanonical NF-kappaB signaling pathways."
NFkappaB activates STAMBPL1.
| 1
| 1

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"Overexpression of wild-type STAMBPL1, but not a catalytically inactive mutant, enhanced Tax mediated NF-kappaB activation, whereas silencing of STAMBPL1 with siRNA impaired Tax activation of both the canonical and noncanonical NF-kappaB signaling pathways."
DEPDC5 affects STAMBPL1
| 2
| 2

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"Knockout of GATOR1 component DEPDC5 or NPRL2 in WT or STAMBPL1-null LS174T cells ( Figures S8 K and S8L) restored anchorage-independent cell growth and xenograft tumor growth in STAMBPL1-deficient cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Knockout of GATOR1 component DEPDC5 or NPRL2 restored mTORC1 activity and promoted anchorage-independent cell growth in STAMBPL1(K405Rfs ∗ 21) mutation-corrected LS174T cells ( Figures 6 G and S9 E–S9[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
CFLAR affects BIRC5
| 2
| 2

sparser
"Thus, these data reveal that STAMBPL1 directly interacts with survivin and c-FLIP, resulting in stabilization of proteins."

sparser
"STAMBPL1 interacted with survivin and c-FLIP, resulted in reduction of ubiquitination."
Sst2 affects STAMBPL1
| 1
| 1

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"In contrast, AMSH-LP and its yeast homologue Sst2 bind K63 diUb with K M ~ 50 muM, and k cat ~ 1 s -1, substantially below the catalytic proficiency associated with cleavage of some small peptides by thermolysin."
| 1

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"The binding energies for each interaction were recorded, and statistical analyses were performed to draw meaningful conclusions regarding the potential binding of STAMBPL1 with sorafenib, regorafenib, lenvatinib, and cabozantinib."
ShSTAMBPL1 affects STAMBPL1
| 1
ShSTAMBPL1 inhibits STAMBPL1. 1 / 1
| 1

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"Furthermore, knockdown of STAMBPL1 by shSTAMBPL1 resulted in STAMBPL1 downregulation, which in turn decreased the cell proliferation of AGS gastric cancer cells, increasing cell apoptosis, cell invasion and migration in vitro."
ShSTAMBPL1#2 affects STAMBPL1
| 1
ShSTAMBPL1#2 decreases the amount of STAMBPL1. 1 / 1
| 1

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"The collective findings demonstrate that shSTAMBPL1#1 and shSTAMBPL1#2 inhibited the expression of STAMBPL1 effectively (Fig. 3C–F)."
ShSTAMBPL1#1 affects STAMBPL1
| 1
ShSTAMBPL1#1 decreases the amount of STAMBPL1. 1 / 1
| 1

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"The collective findings demonstrate that shSTAMBPL1#1 and shSTAMBPL1#2 inhibited the expression of STAMBPL1 effectively (Fig. 3C–F)."
Rpn11 affects Ubiquitin
| 1
Rpn11 binds STAMBPL1 and Ubiquitin. 1 / 1
| 1

sparser
"Rpn11 and AMSH-LP bind ubiquitin in a similar orientation, but their interaction with the Ile44 hydrophobic patch is quite different."
Polyubiquitin affects STAMBPL1
| 1
STAMBPL1 binds polyubiquitin. 1 / 1
| 1

sparser
"Thus, the structure of the AMSH-LP domain bound to K63-linked polyubiquitin reveals one mode by which DUBs discriminate between polyubiquitin isoforms."
Patch affects Ubiquitin
| 1
STAMBPL1 binds patch and Ubiquitin. 1 / 1
| 1

sparser
"AMSH-LP binds the distal ubiquitin via its Leu8/Ile44/Val70 hydrophobic patch conserved in NEDD8."
Ectopic following affects STAMBPL1
| 1
Ectopic following activates STAMBPL1. 1 / 1
| 1

eidos
"A 137 number of novel host factors have been confirmed to facilitate SARS-CoV-2 pseudovirus entry ,138 151HEK293T cells , besides ACE2 and CTSL , the ectopic expression of any of the following genes could 152 effectively enable SARS-CoV-2 infection , CLEC4G , CPLX1 , LDLRAD3 , TMEM30A , and STAMBPL1 153 ( Fig. 3B ) ."
| DOI
DiUb affects STAMBPL1
| 1
DiUb binds STAMBPL1. 1 / 1
| 1

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"(E) A comparison of the diUb/STAMBPL1 complex structure (left) and UbV/STAMBPL1 structure overlaid with diUb (right)."
| DOI
| 1

eidos
"However , although cepharanthine reduces STAMBPL1 expression and overexpression of STAMBPL1 inhibits cepharanthine-mediated downregulation of survivin , interaction between STAMBPL1 and survivin was not investigated ."
YPEL3 affects STAMBPL1
| 1
| 1

sparser
"These data suggest that STAMBPL1, CEP55, YPEL3 and IGFBP1 may be associated with the prognosis of patients with PAAD."
Ubiquitin affects rpn11
| 1
Rpn11 binds STAMBPL1 and Ubiquitin. 1 / 1
| 1

sparser
"Rpn11 and AMSH-LP bind ubiquitin in a similar orientation, but their interaction with the Ile44 hydrophobic patch is quite different."
Ubiquitin affects patch
| 1
STAMBPL1 binds patch and Ubiquitin. 1 / 1
| 1

sparser
"AMSH-LP binds the distal ubiquitin via its Leu8/Ile44/Val70 hydrophobic patch conserved in NEDD8."
Ubiquitin affects BRCC36
| 1

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
UbV affects STAMBPL1
| 1
STAMBPL1 binds UbV. 1 / 1
| 1

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"A strong hydrogen bond network is then formed between the Arg72 guanidinium and the backbone carbonyl group of Glu326 in ins-1 and between the amide group of Gln49 of UbV with the backbone carbonyl and amide of Val328 and Glu326, thus stabilizing the interaction between the STAMBPL1 and UbV (Fig. 2G)."
UbV SP.1 affects STAMBPL1
| 1
UbV SP.1 inhibits STAMBPL1. 1 / 1
| 1

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"In addition, UbV SP.1 appeared to inhibit STAMBPL1 to some extent, whereas UbV SP.3 showed no inhibitory effect with STAMBPL1 (Fig. S2A) ."
| DOI
UBD affects Phe403
| 1
STAMBPL1 binds UBD and Phe403. 1 / 1
| 1

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"X-ray crystal structures of the DUB domains of both AMSH-LP and Sst2 bound to diubiquitin shows Phe403 collapsed onto the Lys63 side chain of the proximal ubiquitin; however, the flap residues appear to retain their mobility even in presence of bound substrate or product (indicated by relatively weak electron density enveloping these residues and higher B-factors than internal residues)."
TRIM21 affects STAMBPL1
| 1

sparser
"However, the interaction of STAMBPL1 and TRIM21 was not detected in KIRC cell lines (786‐O, 769‐P and Caki‐1) (Figure xref , Supporting Information)."
TBMS-1- affects STAMBPL1
| 1
TBMS-1- decreases the amount of STAMBPL1. 1 / 1
| 1

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"Moreover, TBMS-1- or CHX-only treatments downregulated STAMBPL1 protein expression levels from 9 h (Figure 4E,F)."
STAMBPL1 affects survivin c-FLIP protein
| 1
STAMBPL1 activates survivin c-FLIP protein. 1 / 1
| 1

eidos
"Knockdown of STAMBPL1 reduced survivin and c-FLIP protein levels , while overexpression of STAMBPL1 inhibited honokinol-induced survivin and c-FLIP degradation ."
STAMBPL1 affects sst2
| 1
| 1

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"In contrast, AMSH-LP and its yeast homologue Sst2 bind K63 diUb with K M ~ 50 muM, and k cat ~ 1 s -1, substantially below the catalytic proficiency associated with cleavage of some small peptides by thermolysin."
| 1

reach
"The binding energies for each interaction were recorded, and statistical analyses were performed to draw meaningful conclusions regarding the potential binding of STAMBPL1 with sorafenib, regorafenib, lenvatinib, and cabozantinib."
| 1

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"Collectively, our findings suggested that STAMBPL1 contributes to c-FLIP degradation and TRAIL sensitization by TBMS-1."
STAMBPL1 affects rpn11
| 1
Rpn11 binds STAMBPL1 and Ubiquitin. 1 / 1
| 1

sparser
"Rpn11 and AMSH-LP bind ubiquitin in a similar orientation, but their interaction with the Ile44 hydrophobic patch is quite different."

reach
"Multi-omics studies demonstrate that Gamabufotalin exerts anti-HCC effects through STAMBPL1-mediated regulation of amino acid metabolism (Zheng, et al., 2024)."
STAMBPL1 affects polyubiquitin
| 1
STAMBPL1 binds polyubiquitin. 1 / 1
| 1

sparser
"Thus, the structure of the AMSH-LP domain bound to K63-linked polyubiquitin reveals one mode by which DUBs discriminate between polyubiquitin isoforms."
STAMBPL1 affects peptide
| 1
STAMBPL1 bound to SESN2 inhibits peptide. 1 / 1
| 1

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"Inhibiting the interaction between STAMBPL1 and Sestrin2 thereby inactivating mTORC1 using a cell-permeable peptide could be a potential treatment approach for gastric and colorectal cancers [141]."
STAMBPL1 affects pathway
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STAMBPL1 activates pathway. 1 / 1
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sparser
"Mechanistically, STAMBPL1 activated Wnt/β-catenin pathway and increased the expression of downstream cancer-promoting genes."
STAMBPL1 affects patch
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STAMBPL1 binds patch and Ubiquitin. 1 / 1
| 1

sparser
"AMSH-LP binds the distal ubiquitin via its Leu8/Ile44/Val70 hydrophobic patch conserved in NEDD8."
| 1
STAMBPL1 increases the amount of messenger RNA. 1 / 1
| 1

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"qPCR results revealed, beyond the knockdown efficiency, that depletion of STAMBPL1 causes an elevation of CDH1 and a reduction of VIM messenger RNA (mRNA) levels (Fig. 2b)."
| 1

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"Functionally, STAMBPL1 notably enhances CCA cell proliferation and metastasis while impeding ferroptosis."

reach
"STAMBPL1 expression contributes to EMT."
STAMBPL1 affects diUb
| 1
DiUb binds STAMBPL1. 1 / 1
| 1

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"(E) A comparison of the diUb/STAMBPL1 complex structure (left) and UbV/STAMBPL1 structure overlaid with diUb (right)."
| DOI
| 1
| 1

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"Our previous research demonstrated that STAMBPL1 enhances cisplatin resistance in TNBC cells through the stabilization of MKP-1 (Liu et al., 2022)."
STAMBPL1 affects cepharanthine-mediated survivin
| 1
STAMBPL1 activates cepharanthine-mediated survivin. 1 / 1
| 1

eidos
"However , although cepharanthine reduces STAMBPL1 expression and overexpression of STAMBPL1 inhibits cepharanthine-mediated downregulation of survivin , interaction between STAMBPL1 and survivin was not investigated ."
STAMBPL1 affects breast cancer cell resistance cisplatin
| 1
STAMBPL1 activates breast cancer cell resistance cisplatin. 1 / 1
| 1

eidos
"STAMBPL1 promotes breast cancer cell resistance to cisplatin partially by stabilizing MKP-1 expression ."
| 1
| 1

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"We also showed that knockout of STAMBPL1 in HEK293T cells activated the catabolic autophagy pathway ( Figures S7 A and S7B), reduced the cell size ( Figure S7 C), and suppressed anchorage-independent [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 affects YPEL3
| 1
| 1

sparser
"These data suggest that STAMBPL1, CEP55, YPEL3 and IGFBP1 may be associated with the prognosis of patients with PAAD."
STAMBPL1 affects Wnt/β-catenin
| 1
STAMBPL1 activates Wnt/β-catenin. 1 / 1
| 1

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"Mechanistically, STAMBPL1 activated Wnt/β-catenin pathway and increased the expression of downstream cancer-promoting genes."
STAMBPL1 affects WNT/PI3K/NF-kb
| 1
STAMBPL1 activates WNT/PI3K/NF-kb. 1 / 1
| 1

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"Especially STAMBPL1 could modulate the stability of TRAF2 protein and activate WNT/PI3K/NF-kb signalling pathway."
STAMBPL1 affects VIM
| 1
STAMBPL1 increases the amount of VIM. 1 / 1
| 1

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"qPCR results revealed, beyond the knockdown efficiency, that depletion of STAMBPL1 causes an elevation of CDH1 and a reduction of VIM messenger RNA (mRNA) levels (Fig. 2b)."
STAMBPL1 affects Ubiquitin, and rpn11
| 1
Rpn11 binds STAMBPL1 and Ubiquitin. 1 / 1
| 1

sparser
"Rpn11 and AMSH-LP bind ubiquitin in a similar orientation, but their interaction with the Ile44 hydrophobic patch is quite different."
STAMBPL1 affects Ubiquitin, and patch
| 1
STAMBPL1 binds patch and Ubiquitin. 1 / 1
| 1

sparser
"AMSH-LP binds the distal ubiquitin via its Leu8/Ile44/Val70 hydrophobic patch conserved in NEDD8."
STAMBPL1 affects UbV
| 1
STAMBPL1 binds UbV. 1 / 1
| 1

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"A strong hydrogen bond network is then formed between the Arg72 guanidinium and the backbone carbonyl group of Glu326 in ins-1 and between the amide group of Gln49 of UbV with the backbone carbonyl and amide of Val328 and Glu326, thus stabilizing the interaction between the STAMBPL1 and UbV (Fig. 2G)."
STAMBPL1 affects TRIM21
| 1

sparser
"However, the interaction of STAMBPL1 and TRIM21 was not detected in KIRC cell lines (786‐O, 769‐P and Caki‐1) (Figure xref , Supporting Information)."
STAMBPL1 affects TP53
| 1
STAMBPL1 activates mutated TP53. 1 / 1
| 1

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"Concomitantly, we observed that STAMBPL1 expression was elevated in cells with mutant p53-elicited EMT (Fig. 6c)."
STAMBPL1 affects Sestrin2-GATOR2
| 1
STAMBPL1 leads to the ubiquitination of Sestrin2-GATOR2. 1 / 1
| 1

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"Interestingly, Wang et al. reported that gastric cancer and colorectal cancer downregulate an E3 ubiquitin ligase RING finger protein 167 (RNF167) and upregulate a deubiquitinase STAMBPL1 that can prevent the ubiquitination of Sestrin2, decrease Sestrin2-GATOR2 interaction and increase mTORC1 signaling [119]."
STAMBPL1 affects SYT1
| 1
STAMBPL1 activates SYT1. 1 / 1
| 1

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"The ICC/IF experiment showed the expression of STAMBPL1 could mediate the P65 protein to transfer to the nucleus (Fig. 5I, J)."
STAMBPL1 affects STAMBPL1
| 1
STAMBPL1 increases the amount of STAMBPL1. 1 / 1
| 1

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"We further constructed stable STAMBPL1 over-expressed cells (including HCCLM3 and Hep3B), and the efficiency of overexpression STAMBPL1 were examined by qRT-PCR and WB, the results showed that overexpression STAMBPL1 can upregulate the STAMBPL1 expression in HCCLM3 cells and Hep3B cells (Fig. 4A–D)."
STAMBPL1 affects SP1
| 1
| 1

sparser
"While our study highlights and connect three key signalling pathways: mutant p53, EMT and DUBs, showing that STAMBPL1 is transcriptionally elevated in mutant p53-expressing cells, STAMBPL1 seems not to be under a direct regulation by p53 R175H . However, out of the known TFs that help recruit mutant p53 to the chromatin to upregulate genes, xref we found that E2F2, E2F6, MAFF and SP1 can bind to the core promoter region of STAMBPL1 by their motif search using Eukaryotic promoter database."
STAMBPL1 affects SMAD2
| 1
| 1

sparser
"Previous studies reported that although they did not investigate the DUB activity of STAMBPL1, STAMBPL1 interact with Smad2 and Smad7, followed by induction of TGF-β-mediated transcriptional activity [ xref ]."
STAMBPL1 affects S7B
| 1
STAMBPL1 inhibits S7B. 1 / 1
| 1

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"We also showed that knockout of STAMBPL1 in HEK293T cells activated the catabolic autophagy pathway ( Figures S7 A and S7B), reduced the cell size ( Figure S7 C), and suppressed anchorage-independent [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 affects RPN11
| 1
| 1

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"In fact, whereas the Ins-2 loop of AMSH-LP contacts the proximal ubiquitin, restricting linkage specificity [44], in Rpn11, it anchors the enzyme to the proteasome [45,66]."
STAMBPL1 affects RNF167
| 1

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"The binding of RNF167 and STAMBPL1 to Sestrin2 is not mutually exclusive, as the interaction of one protein with Sestrin2 was not affected by deficiency or overexpression of the other protein ( Figure[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 affects Phe403
| 1
STAMBPL1 binds UBD and Phe403. 1 / 1
| 1

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"X-ray crystal structures of the DUB domains of both AMSH-LP and Sst2 bound to diubiquitin shows Phe403 collapsed onto the Lys63 side chain of the proximal ubiquitin; however, the flap residues appear to retain their mobility even in presence of bound substrate or product (indicated by relatively weak electron density enveloping these residues and higher B-factors than internal residues)."
STAMBPL1 affects NSCLC
| 1
STAMBPL1 activates NSCLC. 1 / 1
| 1

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"The results suggested that STAMBPL1 promoting NSCLC progression in A549 and H1299 LUAD cell lines is partly achieved by inhibiting the expression of DHRS2 (Fig. 5B-E)."
STAMBPL1 affects MYC
| 1
STAMBPL1 decreases the amount of MYC. 1 / 1
| 1

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"It is of great interest that the C-terminal-half-deleted mutants of AMSH and AMSH-LP, which lack the JAMM motifs, are unable to induce the IL-2-mediated c- myc expression."
STAMBPL1 affects MAFF
| 1
| 1

sparser
"While our study highlights and connect three key signalling pathways: mutant p53, EMT and DUBs, showing that STAMBPL1 is transcriptionally elevated in mutant p53-expressing cells, STAMBPL1 seems not to be under a direct regulation by p53 R175H . However, out of the known TFs that help recruit mutant p53 to the chromatin to upregulate genes, xref we found that E2F2, E2F6, MAFF and SP1 can bind to the core promoter region of STAMBPL1 by their motif search using Eukaryotic promoter database."
STAMBPL1 affects Lys63-Ub 2
| 1
STAMBPL1 binds Lys63-Ub 2. 1 / 1
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"The 10 best structures of the best cluster in terms of Haddock score were retained.The proposed structure of the STAM2/AMSH/Lys63-Ub 3 ternary complex has been obtained by assembling the SH3/AMSH stru[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 affects Liu et al., 2022
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STAMBPL1 activates Liu et al., 2022. 1 / 1
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reach
"Our previous research revealed that STAMBPL1, a deubiquitinase, promotes cisplatin resistance in TNBC by stabilizing MKP1 (Liu et al., 2022)."
STAMBPL1 affects LUAD
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STAMBPL1 activates LUAD. 1 / 1
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"Cell migration showed that the number of A549 and H1299 cells in the STAMBPL1 SI group was lower than that in A549 STAMBPL1 NC and H1299 STAMBPL1 NC group, indicating that STAMBPL1 promoted cell migration of LUAD (Fig. 2C, p<0.001, p<0.001)."
STAMBPL1 affects IGFBP1
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sparser
"These data suggest that STAMBPL1, CEP55, YPEL3 and IGFBP1 may be associated with the prognosis of patients with PAAD."
STAMBPL1 affects Hypoxia
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reach
"In this study, we discovered that STAMBPL1 could upregulate the expression of the hypoxia-inducible factor HIF1alpha in breast cancer cells."
STAMBPL1 affects GRB2
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sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
STAMBPL1 affects Flag
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sparser
"The Flag-STAMBPL1 plasmid were well constructed and transferred to HCCLM3 and Hep3B cell, including (Control, Flag-STAMBPL1-2ug, Flag-STAMBPL1-3ug, Flag-STAMBPL1-4ug), The findings indicated that elevated STAMBPL1 levels led to a decrease in the ubiquitination levels of TRAF2 in both HCCLM3 and Hep3B cells (Fig.  xref A, B)."
STAMBPL1 affects FBN2
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STAMBPL1 activates FBN2. 1 / 1
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"Functionally, STAMBPL1 notably enhances CCA cell proliferation and metastasis while impeding ferroptosis."
STAMBPL1 affects E2F6
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sparser
"While our study highlights and connect three key signalling pathways: mutant p53, EMT and DUBs, showing that STAMBPL1 is transcriptionally elevated in mutant p53-expressing cells, STAMBPL1 seems not to be under a direct regulation by p53 R175H . However, out of the known TFs that help recruit mutant p53 to the chromatin to upregulate genes, xref we found that E2F2, E2F6, MAFF and SP1 can bind to the core promoter region of STAMBPL1 by their motif search using Eukaryotic promoter database."
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STAMBPL1 deubiquitinates Deubiquitinase on K63. 1 / 1
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reach
"Structure of the Zn-dependent AMSH-LP deubiquitinating enzyme (DUB) in complex with Lys63-linked diubiquitins provided the first evidence for recognition of an isopeptide bond-linked ubiquitin chain [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
STAMBPL1 affects Death
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reach
"Chen et al. reported that the silencing of STAMBPL1 using shRNA triggers caspase-dependent death via XIAP degradation in prostate cancer cells [35]."
STAMBPL1 affects DUB domain
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STAMBPL1 binds K63-Ub 2 and DUB domain. 1 / 1
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"The overall three-dimensional fold of Sst2 Delta220 -K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 A of Calpha atoms over 266 residues from both Sst2 and K63-Ub 2."

reach
"As a result, viral transcription and replication are greatly suppressed by either LEF1 or STAMBPL1, resulting in selective viral replication in LEF1 and STAMPBPL1 low expressing cells."
STAMBPL1 affects DMD
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sparser
"Furthermore, by transcriptomic analysis we were able to identify 10 fusions compatible with inter-chromosomal translocations not previously showed by conventional methods ( PAX5::POM121C, IK::FBXW2, ZNF444::HLA-B, NFX1::DICER1, DCAF8::ZNF836, DMD::STAMBPL1, SLFNL1::SMPD2, RP11-148O21.2::ATG4B, TMEM263::CD47,INPP5A::SETD7 ) and 6 intra-chromosomal events involving distant genes ( NUP214::ABL1, MAEA::CTBP1, ZC3H12D::RP11-445F6.2, MAML2::FAT3, MNT::CLUH, TSKS::ARRDC2 ) ( xref )."

reach
"This study firstly reveals that STAMBPL1 promotes cholangiocarcinoma progression by upregulating NRF2, indicating that targeting the STAMBPL1/NRF2 axis is a novel therapeutic strategy."
STAMBPL1 affects Caspase
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reach
"Chen et al. reported that the silencing of STAMBPL1 using shRNA triggers caspase-dependent death via XIAP degradation in prostate cancer cells [35]."

eidos
"A rescue experiment was conducted to certify that STAMBPL1 promotes NSCLC progression by regulating DHRS2 expression ."
| 1

reach
"STAMBPL1 deficiency attenuates liver tumorigenesis in vitro and in vivo."
STAMBPL1 affects CUL1
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"Recent studies have shown that excessive ROS generated in H. pylori infection induces direct degradation of STAMBPL1 by cullin 1-RING ubiquitin ligase and the 26S proteasome, while STAMBPL1 can lead to cell apoptosis by reducing the anti-apoptotic protein survivin (Chaithongyot and Naumann, 2022)."
STAMBPL1 affects CNTN2
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STAMBPL1 leads to the deubiquitination of CNTN2. 1 / 1
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reach
"The deubiquitination of Tax-1 can be also mediated by STAM binding protein like 1 (STAMBPL1), which was identified in an RNA interference screen XREF_BIBR."
STAMBPL1 affects CLTC
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sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
STAMBPL1 affects CEP55
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sparser
"These data suggest that STAMBPL1, CEP55, YPEL3 and IGFBP1 may be associated with the prognosis of patients with PAAD."
STAMBPL1 affects CDH1
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STAMBPL1 increases the amount of CDH1. 1 / 1
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"These analyses showed that the KO of STAMBPL1 caused a significant restoration in the expression of E-cadherin, both transcriptionally and at protein levels, and a significant vimentin reduction."
STAMBPL1 affects CD274
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STAMBPL1 increases the amount of CD274. 1 / 1
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"In addition, STAMBPL1 enhanced PD-L1 transcription via facilitating nuclear translocation of p65, and knockdown (KD) of STAMBPL1 augmented antitumor effects of PD-1 blockade."
STAMBPL1 affects CCND1
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STAMBPL1 increases the amount of CCND1. 1 / 1
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"Exogenous expression of STAMBPL1 increased the percentage of cells in the S phase and upregulated the expressions of CyclinD1 and Survivin."
STAMBPL1 affects BRCC36
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sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."

eidos
"STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression ."
STAMBPL1 affects ANXA5
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reach
"STAMBPL1 knockdown in both PC3 and DU145 cells significantly increased Annexin V-positive cell population ( Fig. 1 H), as well as accumulation of dead cells in sub-G1 phase ( Fig. 1 I)."
STAMBP affects Clathrin
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sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
STAM2 affects GRB2
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sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
STAM affects STAM2, and STAMBPL1
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sparser
"Another STAM family protein, STAM2, did not bind to AMSH-LP either, although AMSH strongly binds to STAM2 (data not shown)."
STAM affects GRB2
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sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
SP1 affects STAMBPL1
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sparser
"While our study highlights and connect three key signalling pathways: mutant p53, EMT and DUBs, showing that STAMBPL1 is transcriptionally elevated in mutant p53-expressing cells, STAMBPL1 seems not to be under a direct regulation by p53 R175H . However, out of the known TFs that help recruit mutant p53 to the chromatin to upregulate genes, xref we found that E2F2, E2F6, MAFF and SP1 can bind to the core promoter region of STAMBPL1 by their motif search using Eukaryotic promoter database."
SNP rs2862833 affects STAMBPL1
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SNP rs2862833 increases the amount of STAMBPL1. 1 / 1
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"The protective SNP rs2862833, a downstream variant of the FAS gene, increased the expression of FAS (Z-score 10.8, P = 3.51 x10 -27) and the STAMBPL1 and ACTA2 locus (Z-score 38.25, P = 9.81 x10 -198)."
SMAD7 affects STAMBPL1
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sparser
"Previous studies reported that although they did not investigate the DUB activity of STAMBPL1, STAMBPL1 interact with Smad2 and Smad7, followed by induction of TGF-β-mediated transcriptional activity [ xref ]."
RNF167 affects STAMBPL1
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reach
"The binding of RNF167 and STAMBPL1 to Sestrin2 is not mutually exclusive, as the interaction of one protein with Sestrin2 was not affected by deficiency or overexpression of the other protein ( Figure[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
Phe403 affects UBD
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STAMBPL1 binds UBD and Phe403. 1 / 1
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"X-ray crystal structures of the DUB domains of both AMSH-LP and Sst2 bound to diubiquitin shows Phe403 collapsed onto the Lys63 side chain of the proximal ubiquitin; however, the flap residues appear to retain their mobility even in presence of bound substrate or product (indicated by relatively weak electron density enveloping these residues and higher B-factors than internal residues)."
Neoplasm Metastasis decreases the amount of STAMBPL1. 1 / 1
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"The transwell assays were used to evaluate the influence on invasion and metastasis abilities of HCC cells by reducing the expression of STAMBPL1."
NFE2L2 affects STAMBPL1
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sparser
"Particularly, sites 251-436 of STAMBPL1 interact with sites 228-605 of NRF2, facilitating DUB activity and eliminating ubiquitin molecules attached to NRF2, thus protecting it from proteasome-mediated degradation."
MYC affects STAMBPL1
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MYC activates mutated STAMBPL1. 1 / 1
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"Second, defective intracellular trafficking and localization, originating from D406G germline mutation of STAMBPL1 and enhanced by MYC network activation, inhibit cell polarity establishment and cell differentiation, preventing cell cycle exit."
MIOS affects STAMBPL1
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MIOS decreases the amount of STAMBPL1. 1 / 1
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"Knockdown of the GATOR2 subunit Mios completely blocked the effect of RNF167 deficiency or STAMBPL1 overexpression on mTORC1 activity ( Figures S5 A and S5B), which suggests that GATOR2 functions down[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
MAFF affects STAMBPL1
| 1
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sparser
"While our study highlights and connect three key signalling pathways: mutant p53, EMT and DUBs, showing that STAMBPL1 is transcriptionally elevated in mutant p53-expressing cells, STAMBPL1 seems not to be under a direct regulation by p53 R175H . However, out of the known TFs that help recruit mutant p53 to the chromatin to upregulate genes, xref we found that E2F2, E2F6, MAFF and SP1 can bind to the core promoter region of STAMBPL1 by their motif search using Eukaryotic promoter database."
Lys63-Ub 2 affects STAMBPL1
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STAMBPL1 binds Lys63-Ub 2. 1 / 1
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reach
"The 10 best structures of the best cluster in terms of Haddock score were retained.The proposed structure of the STAM2/AMSH/Lys63-Ub 3 ternary complex has been obtained by assembling the SH3/AMSH stru[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
K63-Ub 2 affects DUB domain
| 1
STAMBPL1 binds K63-Ub 2 and DUB domain. 1 / 1
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"The overall three-dimensional fold of Sst2 Delta220 -K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 A of Calpha atoms over 266 residues from both Sst2 and K63-Ub 2."
K63 affects BRCC36
| 1

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
JAG1 affects STAMBPL1
| 1
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reach
"In cultured human gastric adenocarcinoma epithelial cell lines AGS and NCI-N87, H. pylori -induced production of ROS caused the degradation of the deubiquitinylase STAM-binding protein-like 1 (STAMBPL[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
IGFBP1 affects YPEL3
| 1
| 1

sparser
"These data suggest that STAMBPL1, CEP55, YPEL3 and IGFBP1 may be associated with the prognosis of patients with PAAD."
HIF1A affects STAMBPL1
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HIF1A increases the amount of STAMBPL1. 1 / 1
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sparser
"The difference between HIF-1a expression upon FOXO1 silencing should be compared in the presence or absence of STAMBPL1 overexpression to understand if FOXO1 impacts HIF-1a transcription dependently or independently of STAMBPL1."
GSK3 affects STAMBPL1
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GSK3 decreases the amount of STAMBPL1. 1 / 1
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"Consistent with the effect of GSK-3 inhibition to stimulate the transcription of Snail, in this study, we show that GSK-3α/β inhibition can induce a significant STAMBPL1 expression in all cell lines tested, signifying that this stimulation is able to trigger similar STAMBPL1 molecular response in several cell and cancer types and suggest that GSK-3α/β inhibition may converge upon common signalling element in multiple carcinomas to activate STAMBPL1."
GRB2 affects STAMBPL1
| 1
| 1

sparser
"Consistent with this, coimmunoprecipitation analyses revealed that AMSH-LP could not bind to STAM1, STAM2, or Grb2, all of which contain an SH3 domain that interacts with AMSH [2,3,11] ."
Flag affects STAMBPL1
| 1
| 1

sparser
"The Flag-STAMBPL1 plasmid were well constructed and transferred to HCCLM3 and Hep3B cell, including (Control, Flag-STAMBPL1-2ug, Flag-STAMBPL1-3ug, Flag-STAMBPL1-4ug), The findings indicated that elevated STAMBPL1 levels led to a decrease in the ubiquitination levels of TRAF2 in both HCCLM3 and Hep3B cells (Fig.  xref A, B)."
E2F6 affects STAMBPL1
| 1
| 1

sparser
"While our study highlights and connect three key signalling pathways: mutant p53, EMT and DUBs, showing that STAMBPL1 is transcriptionally elevated in mutant p53-expressing cells, STAMBPL1 seems not to be under a direct regulation by p53 R175H . However, out of the known TFs that help recruit mutant p53 to the chromatin to upregulate genes, xref we found that E2F2, E2F6, MAFF and SP1 can bind to the core promoter region of STAMBPL1 by their motif search using Eukaryotic promoter database."
DUB domain affects STAMBPL1
| 1
STAMBPL1 binds K63-Ub 2 and DUB domain. 1 / 1
| 1

reach
"The overall three-dimensional fold of Sst2 Delta220 -K63-Ub 2 is highly similar to the structure of the DUB domain of AMSH-LP bound to K63-Ub 2 (PDB entry 2ZNV) with an rmsd of 1.19 A of Calpha atoms over 266 residues from both Sst2 and K63-Ub 2."
DMD affects STAMBPL1
| 1
| 1

sparser
"Furthermore, by transcriptomic analysis we were able to identify 10 fusions compatible with inter-chromosomal translocations not previously showed by conventional methods ( PAX5::POM121C, IK::FBXW2, ZNF444::HLA-B, NFX1::DICER1, DCAF8::ZNF836, DMD::STAMBPL1, SLFNL1::SMPD2, RP11-148O21.2::ATG4B, TMEM263::CD47,INPP5A::SETD7 ) and 6 intra-chromosomal events involving distant genes ( NUP214::ABL1, MAEA::CTBP1, ZC3H12D::RP11-445F6.2, MAML2::FAT3, MNT::CLUH, TSKS::ARRDC2 ) ( xref )."
Clathrin affects STAMBP
| 1

sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
Caspase affects STAMBPL1
| 1
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reach
"Necroptosis inhibitor Nec-1 and autophagy inhibitor CQ did not efficiently suppress cell death induced by STAMBPL1 targeting; whereas, addition of a caspase 3 inhibitor, Z-DEVD-FMK, to cells markedly [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
CLTC affects STAMBPL1
| 1

sparser
"We further show that AMSH and AMSH-LP bind to the terminal domain of clathrin heavy chain via a novel clathrin-binding site conserved between these proteins."
CEP55 affects YPEL3
| 1
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sparser
"These data suggest that STAMBPL1, CEP55, YPEL3 and IGFBP1 may be associated with the prognosis of patients with PAAD."
CASP8 affects STAMBPL1
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reach
"Taken together, these data indicate that inhibition of STAMBPL1 in prostate cancer cells triggers caspase-dependent apoptosis.Considering that caspase 8 is the predominant initiator caspase in the cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
BRCC36 affects Ubiquitin
| 1

sparser
"Thus, it is likely that BRCC36 and AMSH-LP bind K63-Ub 2 similarly."
BRCC3 affects STAMBPL1
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BRCC3 deubiquitinates STAMBPL1. 1 / 1
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"To identify the DUB associated with NALP7, we over-expressed plasmids expressing several candidate DUBs including BRCC3, which deubiquitinates NALP3, COPS5, STAMBPL1, USP7 and the endosome associated DUBs, STAMBP and USP8."