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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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RPS6KB1 phosphorylates RPS6. 10 / 376
10 | 3 16 165 182
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"While S6 is predominantly phosphorylated by p70S6K in most mammalian adult tissues, phosphorylation of S6 by p90rsk is important during meiosis in mammalian and amphibian oocytes [XREF_BIBR, XREF_BIBR], and to a lesser extent in growth factor stimulated hepatocytes [XREF_BIBR]."
sparser
"Rapamycin effectively inhibited rpS6 phosphorylation by WT S6K1 or WT S6K2 but did not affect rpS6 phosphorylation in cells expressing the activated, rapamycin-resistant forms of S6K1 or S6K2."
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"In LN229 and GAMG glioblastoma cells treated with either non targeting or PTEN targeting siRNA (siNT and siPTEN, respectively), both AD80 and LY-2779964 (LY64) were effective in reducing the S6K1 dependent phosphorylation of the ribosomal protein S6 (rpS6) at 3 hours (XREF_FIG, XREF_SUPPLEMENTARY)."
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"These results demonstrate that, in imatinib resistant KCL22 cells, inhibition of either CK2 or p70S6K almost abolishes rpS6 phosphorylation, while the inhibition of one kinase does not influence the activity of the other."
sparser
"S6K1 phosphorylates ribosomal protein S6 (RPS6), which can increase translation rates by stimulation of ribosome biogenesis facilitated by increased translation of mRNAs containing 5′ (TOP) sequences."
sparser
"It is known that mTOR phosphorylates p70S6K followed by p70S6K-induced phosphorylation of eIF4B and S6 ( xref ) ( xref )."
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"Active mTORC1 phosphorylates p70S6K and, in turn, activated p70S6K phosphorylates RPS6 XREF_BIBR."
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"In LN229 and GAMG glioblastoma cells treated with either non targeting or PTEN targeting siRNA (siNT and siPTEN, respectively), both AD80 and LY-2779964 (LY64) were effective in reducing the S6K1 dependent phosphorylation of the ribosomal protein S6 (rpS6) at 3 hours (XREF_FIG, XREF_SUPPLEMENTARY)."
rlimsp
"S6K1 phosphorylates ribosomal protein S6 (rpS6) leading to increased translation of ribosomal and transcription factor mRNA resulting in protein translation initiation."
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"mTORC1 phosphorylates the p70S6 kinase (p70S6K), which in turn phosphorylates the S6 ribosomal protein and 4E-BP1, leading to protein translation."
RPS6KB1 phosphorylates RPS6 on S240. 7 / 7
1 | 1 5
sparser
"Similarly, p70 S6 kinase-mediated phosphorylation of ribosomal S6 at S235 and S240 was comparable between WT and Dapk −/− T cells ( xref )."
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"These results are consistent with the conclusion that phosphorylation of S6 (S240) by S6K1 is an essential prerequisite for subsequent phosphorylation of S6 (S235) by RSK."
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"Similarly, p70 S6 kinase mediated phosphorylation of ribosomal S6 at S235 and S240 was comparable between WT and Dapk -/- T cells (XREF_SUPPLEMENTARY)."
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"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [XREF_BIBR]."
biopax:phosphositeplus
No evidence text available
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"The p70 ribosomal S6 kinases, directly regulated by mTOR, phosphorylate rpS6 on Ser 240 and Ser 244."
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"The ribosomal protein S6 is a component of the 40S ribosomal subunit and is phosphorylated at Ser 240/244 by p70S6 kinase (S6K1), a direct target of mTORC1."
Phosphorylated RPS6KB1 phosphorylates RPS6. 7 / 7
1 | 6
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"Activated mTOR phosphorylates p70 ribosomal protein S6 kinase (p70S6K) followed by p70S6K induced phosphorylation of ribosomal protein S6 and eukaryotic initiation factor 4B (eIF-4B) which promotes the initiation of protein translation."
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"It is known that mTOR phosphorylates p70S6K followed by p70S6K induced phosphorylation of eIF4B and S6 (XREF_FIG)."
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"It has been well established that activation of PI3K/Akt/mTOR pathway leads to phosphorylation of p70S6 kinase, which subsequently induces the phosphorylation of ribosomal protein S6."
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"Both the phosphorylated p70S6K and phosphorylated rpS6 expression were reduced in the FulvC2 as compared to the parental and other sublines."
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"In particular, phosphorylated p70S6K enhances phosphorylation and activity of ribosomal protein S6 and initiates various translation activities required for cell growth."
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"Phosphorylation of p70S6K stimulates its phosphorylation of ribosomal protein S6, initiating a variety of translation associated activities."
biopax:panther
No evidence text available
RPS6KB1 phosphorylates RPS6 on serine. 6 / 6
| 1 3 2
sparser
"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [ xref ]."
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"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [XREF_BIBR]."
rlimsp
"Ribosomal protein (rp) S6 is a component of the 40S ribosomal subunit that becomes phosphorylated at several serine residues upon mitogen stimulation, but the exact molecular mechanisms regulating its phosphorylation and the function of phosphorylated rpS6 is poorly understood. Here, we provide evidence that activation of the p90 ribosomal S6 kinases (RSKs) by serum, growth factors, tumor promoting phorbol esters, and oncogenic Ras is required for rpS6 phosphorylation downstream of the Ras/ERK signaling cascade. We demonstrate that while ribosomal S6 kinase 1 (S6K1) phosphorylates rpS6 at all sites, RSK exclusively phosphorylates rpS6 at Ser(235/236) in vitro and in vivo using an mTOR-independent mechanism."
sparser
"In T cells, rpS6 is phosphorylated on five evolutionarily conserved serine residues by S6K1 and to a lesser extent by other AGC kinases including the p90 ribosomal S6 kinases ( xref ) in response to TCR/costimulation and cytokine and nutrient signaling pathways."
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"p70S6K subsequently phosphorylates the S6 ribosomal protein on Ser (235)/Ser (236) and Ser (240)/Ser (244) to promote initiation of mRNA translation."
sparser
"In T cells, rpS6 is phosphorylated on 5 evolutionarily conserved serine residues by S6K1, and to a lesser extent by other AGC kinases including the p90 ribosomal S6 kinases (RSKs) ( xref ), in response to TCR/costimulation and cytokine and nutrient signaling pathways."
RPS6KB1-S6K phosphorylates RPS6. 5 / 6
| 1 5
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"p70 S6K phosphorylates eukaryotic translation Initiation Factor-4B (eIF-4B) and S6 Ribosomal Protein (S6RP), which in turn allows translation and translational elongation."
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"p70 S6K phosphorylates the ribosomal protein S6 (rpS6), resulting in increased translation of mRNAs containing a 5 ' olygopyrimidine tract, whereas phosphorylation of 4E-BP1 by mTOR relieves inhibition on the initiation factor eIF4E resulting in more efficient cap dependent translation XREF_BIBR."
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"p70 S6K phosphorylates the ribosomal protein S6 (RPS6), resulting in increased translation of mRNAs containing a 5 ' oligopyrimidine tract, whereas phosphorylation of 4E-BP1 by mTOR relieves inhibition on the initiation factor eIF4E, resulting in more efficient cap dependent translation."
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"As mentioned above, the phosphorylation status of S6 is also commonly used as a marker of mTOR activity, and previous studies have shown that S6 is predominantly phosphorylated by p70 s6k on residues such as S235/236 and S240/244 XREF_BIBR, XREF_BIBR."
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"Upon activation by receptor tyrosine kinases or G protein coupled receptors, AKT activates mTOR, which in turn stimulates protein translation through inhibitory phosphorylation of eukaryotic translational initiation factor eIF4E binding protein 1 (4E-BP1) that inhibits eIF4E, and activating phosphorylation of p70S6 kinase (S6K) that phosphorylates ribosomal S6 protein."
RPS6KB1 phosphorylates RPS6 on S244. 4 / 4
1 | 3
biopax:phosphositeplus
No evidence text available
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"The p70 ribosomal S6 kinases, directly regulated by mTOR, phosphorylate rpS6 on Ser 240 and Ser 244."
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"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [XREF_BIBR]."
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"The ribosomal protein S6 is a component of the 40S ribosomal subunit and is phosphorylated at Ser 240/244 by p70S6 kinase (S6K1), a direct target of mTORC1."
RPS6KB1 phosphorylates RPS6 on S235. 4 / 4
1 1 | 1 1
biopax:phosphositeplus
No evidence text available
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"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [XREF_BIBR]."
sparser
"Similarly, p70 S6 kinase-mediated phosphorylation of ribosomal S6 at S235 and S240 was comparable between WT and Dapk −/− T cells ( xref )."
signor
"A knockin mouse carrying mutations at all phosphorylation sites in the primary s6k substrate, ribosomal protein s6 (rps6), has provided insight into the physiological role of this protein phosphorylation event. Of the many known substrates of s6k1, it is rps6 that has been shown to be directly involved, via its phosphorylation, in controlling cell size."
RPS6KB1 phosphorylates RPS6 on S236. 4 / 4
1 1 | 2
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"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [XREF_BIBR]."
biopax:phosphositeplus
No evidence text available
signor
"A knockin mouse carrying mutations at all phosphorylation sites in the primary s6k substrate, ribosomal protein s6 (rps6), has provided insight into the physiological role of this protein phosphorylation event. Of the many known substrates of s6k1, it is rps6 that has been shown to be directly involved, via its phosphorylation, in controlling cell size."
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"XREF_BIBR The S6K1 dependent regulation of cellular size is mediated by ribosomal protein S6, which is phosphorylated by S6K1 and the closely related S6K2 on Ser235 and Ser236."
RPS6KB1 phosphorylates RPS6 on S6. 3 / 3
| 3
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"The IFNgamma activated p70 S6 kinase subsequently phosphorylates the 40S S6 ribosomal protein on serines 235/236, to regulate IFNgamma dependent mRNA translation."
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"Activated mTORC1 phosphorylates S6K1, which phosphorylates S6 (40S ribosomal protein S6), enhancing the translation of mRNAs with a 5 '-terminal oligopolypyrimidine (5 '-TOP)."
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"Subsequently, activated p70S6K phosphorylates S6 (40S ribosomal protein S6) and other ribosomal proteins and elongation factors and therefore enhances the translation of mRNAs and promotes proteins synthesis XREF_BIBR."
Kinase-active RPS6KB1 phosphorylates RPS6 on S244. 2 / 2
2 |
bel
"from full text:Phosphorylation of rpS6 is mediated by both S6K1 and p90RSK, whereby S6K1 phosphorylates two sets of sites, S235/236 and S240/244, and p90RSK phosphorylates only S235/236"
bel
"we found that S6K1, but not RSK1 or RSK2, was capable of phosphorylating rpS6 on Ser240/244."
Kinase-active RPS6KB1 phosphorylates RPS6 on S240. 2 / 2
2 |
bel
"we found that S6K1, but not RSK1 or RSK2, was capable of phosphorylating rpS6 on Ser240/244."
bel
"from full text:Phosphorylation of rpS6 is mediated by both S6K1 and p90RSK, whereby S6K1 phosphorylates two sets of sites, S235/236 and S240/244, and p90RSK phosphorylates only S235/236"
Modified RPS6KB1 leads to the phosphorylation of RPS6. 1 / 1
| 1
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"Both S6K1 and S6K2 are expressed in T cells and contribute to robust phosphorylation of S6 and other substrates following TCR stimulation."
Phosphorylated RPS6KB1 phosphorylates RPS6 on S6. 1 / 1
| 1
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"The phosphorylated S6K1 then phosphorylates S6 (40S ribosomal protein S6), thereby enhancing the translation of mRNAs."
RPS6KB1 phosphorylates RPS6 on threonine. 1 / 1
| 1
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"Protein, p70S6K, accompanied with Ser Thr kinase, phosphorylates the ribosomal S6 subunit, the fundamental sequel in cell cycle control, growth, and differentiation leading to tau accumulation by translation and upregulating the expression."
RPS6KB1 phosphorylates RPS6 on S247. 1 / 1
| 1
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"After stimulation, S6K1 phosphorylates S6 on five serine residues (S235, S236, S240, S244, and S247) within the C-terminus [XREF_BIBR]."