IndraLab

Statements

EGFR phosphorylates itself. 10 / 1875
| 26 1849
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"Even at 0.01 micromol/L, ZD1839 inhibited autophosphorylation of HER1 by EGF."
16267010
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"Inhibition of EGF receptor autophosphorylation by 30 uM A77 1726."
8224241
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"Dacomitinib irreversibly inhibited EGFR autophosphorylation in the A431 squamous cell carcinoma cell line with IC 50 of 15.1 nmol/L alone or in the presence of EGF."
25110867
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"For example, GM3 inhibits the autophosphorylation of purified EGFR reconstituted into the proteoliposomes of defined lipid compositions, but not the EGF binding [360] ."
25969757
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"Efficient internalization of EGFR was observed in EGF-stimulated cells lacking Cul3, as well as normal autophosphorylation and ubiquitination of EGFR."
23739092
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"Phosphoamino acid analysis of the autophosphorylated EGF-R indicated that tyrosine residues were phosphorylated in each preparation."
3012222
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"Incubation of A431 cells with Tyrphostin AG1478 at a dose of 1 μM completely inhibited EGF-induced EGFR autophosphorylation ( Fig. 7 )."
12381374
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"We observed that afatinib blocked EGFR autophosphorylation and also reduced Oct4 expression in HCC827 CSCs ( xref )."
28787001
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"We then monitored EGFR autophosphorylation in serum-starved T-HEp3 and D-HEp3 cells using direct Western blotting with antiactive EGFR antibodies (Figure 1C) or EGFR immunoprecipitation followed by [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12124174
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"Autophosphorylation of solubilized EGF receptor kinase was clearly inhibited by 0.1 microM BE-23372M. Autophosphorylation of EGF receptor in A431 cells was also inhibited."
8188523
EGFR phosphorylates itself on Y1092. 10 / 45
| 1 44
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"For example, Okabe et al. [ xref ] demonstrated different patterns of EGFR autophosphorylation in the Y1068 residue, a key residue representing activation status of EGFR in NSCLC cell lines, including primary cells from Japanese patients with NSCLC."
28352061
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"Our data show that the presence of phosphorylated Fas significantly promoted autophosphorylation of EGFR at Y1068, possibly by facilitating EGFR dimerization, and thus the increased EGFR association with STAT3."
30127519
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"Receptor activation was monitored as a function of EGFR autophosphorylation on Tyr1068 in intact MDA-MB-231 cells ( xref and xref .)."
25790232
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"Exposure of 18Co cells to either TNF-α or LPA alone had no effect on EGFR autophosphorylation at Y1068."
23688423
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"Five minutes after EGF addition, EGFR auto-phosphorylation on Tyr 1068 and Tyr 1045 was 2.7- and 1.8-fold greater, respectively, in SEPW1-overexpressing cells compared to empty vector-transfected cont[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25721765
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"IR caused EGFR tyrosine phosphorylation, whereas it did not induce EGFR autophosphorylation at Tyr992, Tyr1045, and Tyr1068 or Src-dependent EGFR phosphorylation at Tyr845."
16414009
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"EGFR was IPed from the cell lysates and IBed for total EGFR and receptor auto-phosphorylation on Y1173 and Y1068. xref shows that the L858R MT EGFR of H3255 cells is constitutively phosphorylated on Y1173 and Y1068, as expected ( xref ), which is further increased by both EGF and CS treatments, and that Erlotinib treatment could abolish the receptor auto-phosphorylation on Y1173 and Y1068 in control and EGF stimulation conditions."
23686837
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"PP1 used at higher concentration (500 nM) also inhibits EGF induced auto-phosphorylation of EGFR at Tyr 1068 residue (data not shown) and the dissociation of the EGF/EGFR activating pathways could not be studied."
27564112
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"ZD1839 also suppressed EGF-induced stimulation of EGFR autophosphorylation on Y1086 and Y1068, MAPK phosphorylation on T402 and Y404, and Pak1 activity in a dose-dependent manner."
12700278
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"Inhibition of EGFR Signaling Incubation of either glioma cell line with EKI-785, alone or in combination with rapamycin, led to a significant attenua­tion of EGFR Tyr-1068 autophosphorylation in con[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16242075
EGFR phosphorylates itself on tyrosine. 10 / 38
| 1 37
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"Upon ligand binding, EGFR dimerisation undergoes autophosphorylation on multiple tyrosine (Y) residues within the cytoplasmic domain of EGFR, such as EGFR-Y992, -Y1045, -Y1068, and -Y1173."
27387133
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"The binding of EGF to its receptor induces dimerization of receptor subunits and stimulation of the tyrosine kinase activity of EGFR, and results in autophosphorylation of EGFR on specific tyrosine residues."
18594017
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"Ligand-bound EGFR autophosphorylates its cytoplasmic tyrosine residue, which can serve as a docking site for proteins like STAT3 that possess Src Homology 2 (SH2) domain [ xref ]."
30400136
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"In A431 cells, the EGF receptor autophosphorylates tyrosine residues and allows the recruitment of PLC-γ1 through interactions with the SH2 domains present on PLCγ1."
9247120
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"Upon activation by its growth factor ligands, EGFR autophosphorylates at several tyrosine residues."
25390346
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"This results in EGFR autophosphorylation in several tyrosine (Y) residues, including Y992, Y1045, Y1068, Y1148 and Y1173 xref ."
28892308
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"The autophosphorylation of the EGF receptor on multiple tyrosine residues is the critical step initiating the propagation of the signal inside the cell ( Hunter, 2000; Pawson and Nash, 2003; Schlessin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17081983
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"The anti-invasive effect of oxidized streptolysin O on MDA-MB231 breast cancer cells is characterized by its ability to activate the autophosphorylation of EGFR selectively on tyrosine residues besides Y845, indicating that, in this case, Y845 may not be involved in the anti-cancer malignancy [ xref ]."
23702846
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"The autophosphorylation of EGFR at tyrosine residues in human hepatocellular carcinoma HepG2 cells (ATCC, Rockville, MD, USA) was determined by the method of Yang et al. [17] ."
11755203
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"EGFR undergoes autophosphorylation on five high affinity tyrosine residues localized to the C-terminus of the receptor."
7926042
EGFR phosphorylates itself on Y1016. 10 / 16
| 16
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"Treatment of pancreatic carcinoma cells with purified EFEMP1 activates autophosphorylation of EGFR at the positions Tyr-992 and Tyr-1068, but not at the position Tyr-1048."
19804359
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"EGFR autophosphorylation of Y992 provides a docking site for PLC γ [ xref , xref ] whose activation is a prerequisite to EGFR-mediated cell migration [ xref , xref ]."
21649524
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"These combined data indicate that in lung epithelial cells, PTPµ selectively restrains EGFR autophosphorylation of Y845, Y992, Y1068, and Y1086."
18952113
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"By contrast, proproliferative GIV-FA cells showed attenuated EGFR autophosphorylation at Y992, Y1045, and Y1068 but sustained phosphorylation at Y845 ( xref , B and C; and Supplemental Figure S3B)."
20462955
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"Overexpression of PTP μ in EGF-treated cells decreased EGFR autophosphorylation of both Y992 (58% reduction) ( xref , lane 4 vs 3) and Y1068 (42% reduction) ( xref , lane 4 vs 3)."
21649524
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"IR caused EGFR tyrosine phosphorylation, whereas it did not induce EGFR autophosphorylation at Tyr992, Tyr1045, and Tyr1068 or Src-dependent EGFR phosphorylation at Tyr845."
16414009
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"We evaluated the tandem SH2 domains from wild-type phospholipase C γ1 (PLCγ1) fused to enhanced green fluorescent protein (EGFP), hereafter denoted as tSH2-WT, as a biosensor for studying the dynamics of EGFR autophosphorylation at Tyr 992 ."
31164479
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"These results suggest that autophosphorylation of EGFR at tyrosine 992 is indispensable for EGF-induced membrane ruffle formation."
12586341
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"Promigratory GIV-wt cells displayed enhanced EGFR autophosphorylation at Y992, Y1045, and Y1068, whereas Src-dependent phosphorylation at Y845 was similar to controls ( xref , B and C; and Supplemental Figure S3B)."
20462955
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"We show that in highly motile GIV-wt cells with an intact GEF motif, EGFR autophosphorylation at Y992, Y1045, and Y1068 and the corresponding adaptor recruitment (PLCγ, cCbl, and Grb2) are enhanced, whereas in rapidly proliferating, GEF-deficient GIV-FA cells these events are suppressed."
20462955
EGFR phosphorylates itself on Y1197. 10 / 11
| 11
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"This inhibitory effect of E-cadherin ligation on the mitogenic activity of EGFR was associated with a selective inhibition of EGFR phosphorylation at Tyr845 whereas no inhibition was observed for EGFR[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21665310
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"In addition, we observed here that HPV8E6 selectively enhances the EGFR autophosphorylation of Y1173 beyond that of Y1068."
30875834
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"The cells were treated with the growth factors for 2 minutes, the unbound factors were washed away, and the EGFR examined by immunoblotting cell extracts with an antibody that recognizes the receptor autophosphorylated on tyrosine 1173."
21640162
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"We showed the activation model of EGFR in the EGFR-nonaddictive H522 cells lacking c-Src activation, in which the autophosphorylation of EGFR at tyrosine 1173 was paralleled with the phosphorylated tyrosine 1068, 1086, and 1148 with the epidermal growth factor (EGF) addition ( xref ), while the phosphorylation of tyrosine 845, 1101, 974, and 992 was undetectable ( xref )."
34367939
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"Specifically, we showed that in old hepatocytes there is a decrease in autophosphorylation of EGF receptor at Tyr-1173."
23604425
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"EGFR was IPed from the cell lysates and IBed for total EGFR and receptor auto-phosphorylation on Y1173 and Y1068. xref shows that the L858R MT EGFR of H3255 cells is constitutively phosphorylated on Y1173 and Y1068, as expected ( xref ), which is further increased by both EGF and CS treatments, and that Erlotinib treatment could abolish the receptor auto-phosphorylation on Y1173 and Y1068 in control and EGF stimulation conditions."
23686837
sparser
"Mechanistically, FBLN7 binds to the epidermal growth factor receptor (EGFR) through its EGF-like domain, together with the EGF-like calcium-binding domain, and induces EGFR autophosphorylation at tyrosine (Y) 1068 and Y1173, which activates downstream focal adhesion kinase/AKT signaling, thereby leading to fibroblast-to-myofibroblast transdifferentiation."
37344348
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"Western blot analyses indicated that treatment of trophoblastic SGHPL-5 cells and purified term trophoblasts with potentially EGF-contaminated hCG (hCG-A) resulted in auto-phosphorylation of the EGF receptor at tyrosine 1173 whereas supplementation of another urine-purified hCG preparation (hCG-B), recombinant holo-hCG or recombinant alphahCG had no effects."
16620962
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"In line with the result showing that EGF-elicited MAPK phosphorylation was not enhanced by LPC, EGF-induced autophosphorylation of EGFR at Tyr1173, which is involved in MAPK activation, was not affected by LPC ( xref D and E)."
23772401
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"PRMT5 monomethylates EGFR receptor at R1175 to increase trans- autophosphorylation of EGFR at Tyr (Y) 1173 [ xref ], which in turn provides a docking site for the SH2 domain of the phosphatase SHP1 (also known as PTPN6) ( xref )."
26305020
EGFR phosphorylates itself on Y1069. 9 / 9
| 1 8
trips
"Mechanistically, we demonstrated that rLZ-8 bound to EGFR to induce EGFR autophosphorylation at tyrosine1045 and trigger ubiquitination by inducing the formation of EGFR/Cbl complexes, resulting in the degradation of EGFR; however, Cbl-shRNA abolished rLZ-8-induced EGFR degradation."
28198003
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"Mechanistically, we demonstrated that rLZ-8 bound to EGFR to induce EGFR autophosphorylation at tyrosine1045 and trigger ubiquitination by inducing the formation of EGFR/Cbl complexes, resulting in the degradation of EGFR; however, Cbl-shRNA abolished rLZ-8-induced EGFR degradation."
28198003
sparser
"Promigratory GIV-wt cells displayed enhanced EGFR autophosphorylation at Y992, Y1045, and Y1068, whereas Src-dependent phosphorylation at Y845 was similar to controls ( xref , B and C; and Supplemental Figure S3B)."
20462955
sparser
"We show that in highly motile GIV-wt cells with an intact GEF motif, EGFR autophosphorylation at Y992, Y1045, and Y1068 and the corresponding adaptor recruitment (PLCγ, cCbl, and Grb2) are enhanced, whereas in rapidly proliferating, GEF-deficient GIV-FA cells these events are suppressed."
20462955
sparser
"It was notable that IGF1R knockdown influenced not only EGFR autophosphorylation on Y1045, 1068, 1086, 1145 and 1173, but also the phosphorylation of Y845, a Src-mediated transphosphorylation event ."
17320820
sparser
"By contrast, proproliferative GIV-FA cells showed attenuated EGFR autophosphorylation at Y992, Y1045, and Y1068 but sustained phosphorylation at Y845 ( xref , B and C; and Supplemental Figure S3B)."
20462955
sparser
"Five minutes after EGF addition, EGFR auto-phosphorylation on Tyr 1068 and Tyr 1045 was 2.7- and 1.8-fold greater, respectively, in SEPW1-overexpressing cells compared to empty vector-transfected cont[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25721765
sparser
"IR caused EGFR tyrosine phosphorylation, whereas it did not induce EGFR autophosphorylation at Tyr992, Tyr1045, and Tyr1068 or Src-dependent EGFR phosphorylation at Tyr845."
16414009
sparser
"Although further work is needed to define the precise molecular mechanisms of EGFR internalization and degradation, the accumulated evidence suggests the following model of EGF-induced EGFR endocytosis: EGFR that is autophosphorylated at Y1045 within its C-terminal domain recruits and interacts with the TKB domain of c-Cbl."
19489726
EGFR phosphorylates itself on Y1110. 6 / 6
| 6
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"Serum dependent cyclin D1 expression in MCA is associated with Y1086 EGFR autophosphorylation."
19935697
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"These combined data indicate that in lung epithelial cells, PTPµ selectively restrains EGFR autophosphorylation of Y845, Y992, Y1068, and Y1086."
18952113
sparser
"ZD1839 also suppressed EGF-induced stimulation of EGFR autophosphorylation on Y1086 and Y1068, MAPK phosphorylation on T402 and Y404, and Pak1 activity in a dose-dependent manner."
12700278
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"This arrest was linked to epidermal growth factor receptor (EGFR)-Y1086 autophosphorylation, which inhibited AKT activation and cyclin D1 induction xref ."
25118602
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"In addition, ZD1839 suppressed EGF-induced stimulation of EGFR autophosphorylation on Y1086 and Grb2-binding Y1068 sites, c-Src phosphorylation on Y215, and Pak1 activity."
14760089
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"EGFR autophosphorylation of Y1086 provides the dominant but not exclusive docking site for the adaptor molecule Shc, which can, through its interaction with Grb2, elicit ERK1/2 activation [ xref ]."
21649524
EGFR phosphorylates itself on Y869. 4 / 4
| 4
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"It caused EGFR down-regulation and inhibited EGFR Tyr-845 autophosphorylation in both Du145 and PC-3 cells."
20651333
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"These combined data indicate that in lung epithelial cells, PTPµ selectively restrains EGFR autophosphorylation of Y845, Y992, Y1068, and Y1086."
18952113
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"We show that EGFR is capable of direct autophosphorylation of tyrosine 845, which is located on its kinase activation loop, and that the kinase activity of EGFR is ~500-fold higher in the presence of EGF vs. the inhibitory anti-EGFR antibody Cetuximab."
19518076
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"We show that EGFR is capable of direct autophosphorylation of tyrosine 845, which is located on its kinase activation loop, and that the kinase activity of EGFR is approximately 500-fold higher in the presence of EGF vs the inhibitory anti-EGFR antibody cetuximab."
19518076
EGFR phosphorylates itself on Y1172. 2 / 2
| 2
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"A previous study has been shown that PRMT5 regulates the autophosphorylation of EGFR at Tyr1068 and Tyr1172 to promotes EMT in pancreatic cancer cells [ xref ]."
33495409
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"Moreover, previous study proved that PRMT5‐mediated EGFR Arg1175 methylation positively modulates EGF‐induced EGFR trans‐autophosphorylation at Tyr 1173, but have no effect on EGFR trans‐autophosphorylation at Tyr 1086, 845, 992, 1045 and 1148. xref Herein, we for the first time find that PRMT5 promotes the autophosphorylation of EGFR at Y1068 and Y1172 to activate Akt‐β‐catenin axis in pancreatic cancer cells."
31851779
EGFR phosphorylates itself on Y978. 1 / 1
| 1
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"Autophosphorylation of EGFR at Y954 facilitated homodimerization and enhanced downstream Ca"
33096081
EGFR phosphorylates itself on Y998. 1 / 1
| 1
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"In addition to tyrosine phosphorylation of protein substrates, the EGFR can be auto-phosphorylated at Y 974 , Y 992 , Y 1045 , Y 1068 , Y 1086 , Y 1114 , Y 1148 and Y 1173."
33449573