IndraLab

Statements

EGFR phosphorylates itself on Y1092. 45 / 45
| 1 44
sparser
"For example, Okabe et al. [ xref ] demonstrated different patterns of EGFR autophosphorylation in the Y1068 residue, a key residue representing activation status of EGFR in NSCLC cell lines, including primary cells from Japanese patients with NSCLC."
28352061
sparser
"Our data show that the presence of phosphorylated Fas significantly promoted autophosphorylation of EGFR at Y1068, possibly by facilitating EGFR dimerization, and thus the increased EGFR association with STAT3."
30127519
sparser
"Receptor activation was monitored as a function of EGFR autophosphorylation on Tyr1068 in intact MDA-MB-231 cells ( xref and xref .)."
25790232
sparser
"Exposure of 18Co cells to either TNF-α or LPA alone had no effect on EGFR autophosphorylation at Y1068."
23688423
sparser
"Five minutes after EGF addition, EGFR auto-phosphorylation on Tyr 1068 and Tyr 1045 was 2.7- and 1.8-fold greater, respectively, in SEPW1-overexpressing cells compared to empty vector-transfected cont[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25721765
sparser
"IR caused EGFR tyrosine phosphorylation, whereas it did not induce EGFR autophosphorylation at Tyr992, Tyr1045, and Tyr1068 or Src-dependent EGFR phosphorylation at Tyr845."
16414009
sparser
"EGFR was IPed from the cell lysates and IBed for total EGFR and receptor auto-phosphorylation on Y1173 and Y1068. xref shows that the L858R MT EGFR of H3255 cells is constitutively phosphorylated on Y1173 and Y1068, as expected ( xref ), which is further increased by both EGF and CS treatments, and that Erlotinib treatment could abolish the receptor auto-phosphorylation on Y1173 and Y1068 in control and EGF stimulation conditions."
23686837
sparser
"PP1 used at higher concentration (500 nM) also inhibits EGF induced auto-phosphorylation of EGFR at Tyr 1068 residue (data not shown) and the dissociation of the EGF/EGFR activating pathways could not be studied."
27564112
sparser
"ZD1839 also suppressed EGF-induced stimulation of EGFR autophosphorylation on Y1086 and Y1068, MAPK phosphorylation on T402 and Y404, and Pak1 activity in a dose-dependent manner."
12700278
sparser
"Inhibition of EGFR Signaling Incubation of either glioma cell line with EKI-785, alone or in combination with rapamycin, led to a significant attenua­tion of EGFR Tyr-1068 autophosphorylation in con[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16242075
sparser
"NEU1 overexpression diminished EGF-stimulated EGFR Tyr-1068 autophosphorylation by up to 44% but enhanced MUC1-dependent Pseudomonas aeruginosa adhesion by 1.6-1.7-fold and flagellin-stimulated ERK1/2 activation by 1.7-1.9-fold."
22247545
sparser
"As shown in xref , both cetuximab and gefitinib clearly inhibited EGFR kinase activity, and gefitinib had a greater inhibitory effect at the doses used in the experiment, evidenced by a greater reduction in the level of EGFR Y1068 autophosphorylation."
27450723
sparser
"Specifically, forced expression of CD317 led to a strong increase in the auto-phosphorylation of EGFR at Tyr1068, as well as Src-mediated phosphorylation of EGFR at Tyr845 ( xref )."
30890618
sparser
"Inhibition of EGFR autophosphorylation on Y1068 in the HER2 cells was unexpected as this site was previously reported to promote Ras activation by acting as the major Grb2-SOS complex docking site."
23027125
sparser
"While PP1 at 250 nM concentration minimaly affected the EGF induced auto-phosphorylation of EGFR at Tyr 1068 residue (Figure xref )."
27564112
sparser
"In pancreatic adenocarcinomas, Fibulin-3 binds EGFR (competitive to EGF) causing autophosphorylation of EGFR at Tyr-992 and Tyr-1068 and the subsequent phosphorylation of AKT at Thr-308 and ERK at Thr-202 and Tyr-204 and, thus, accelerates pancreatic adenocarcinoma growth xref ."
23936443
sparser
"Treatment of cells from four GBM PDXs with comparable EGFRvIII expression levels ( xref ) and two low background level EGFR expressing and non-activated control GBM PDX cultures with 1 or 10 μM of Gefitinib (reversible Type I), Canertinib (irreversible Type I), Lapatinib (reversible Type II) and Neratinib (irreversible Type II) led to similar levels of EGFR kinase activity inhibition (up to >90%) as measured by the levels of EGFR autophosphorylation at Y1068 in cells from EGFRvIII-positive PDXs but not in the controls ( xref , xref )."
33707748
sparser
"EGFR auto-phosphorylation at tyrosine (Tyr) 1068 upon EGF stimulation of control-siRNA cells increased in both cell lines (Fig.  xref , left panel) but it decreased upon E-cadherin silencing with both E-cadherin siRNAs, used either separately or pooled."
29996940
sparser
"Autophosphorylation of EGFR on Tyr1068 leads to an increase in Akt and STAT3/5 activation, both of which are associated with increased NSCLC survival xref ."
23251519
sparser
"Similar to HAdV-C2 (see xref ), HAdV-B7 and HAdV-E4 both triggered EGFR autophosphorylation at Tyr1068, and NFκB-p65 phosphorylation at Thr254, within the first hour of infection ( xref )."
31425554
sparser
"However, they also found that overexpression of Neu1 using recombinant adenovirus (Ad) encoding FLAG-tagged human NEU1 (Ad-NEU1) diminished EGF-stimulated EGFR Tyr-1068 autophosphorylation by up to 44% but instead, enhanced MUC1-dependent Pseudomonas aeruginosa adhesion by about 2-fold and flagellin-stimulated ERK1/2 activation by nearly 2-fold."
27029067
sparser
"According to our review of the relevant literature, the autophosphorylation of EGFR on Y1068 can lead to the activation of the granzyme B 2-RAS-RAF-MEK1-ERKs and Janus activated kinase-signal transducer and activator of transcription 3 pathways [ xref ]."
28787001
sparser
"In these same infected ECs, EGFR Y1068 autophosphorylation also was evident at MOls ≥10 whereas barrier function was lost only at MOls ≥200."
18952113
sparser
"We first showed that EGFR was autophosphorylated at Tyr1068 in response to TNF-α stimulation in the reconstituted cells ( xref )."
31425554
sparser
"EGFR Tyr-1068 autophosphorylation is required for the receptor-mediated activation and phos­phorylation of the STAT3 transcription factor [9,24], and, not surprisingly, EKI-785 effectively blocked STA[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16242075
sparser
"Consistently, all these flavonoids repress EGFR autophosphorylation at Tyr 1068 and phosphorylation of downstream transcription factor ELK1 after EGF treatment ( xref )."
30364229
sparser
"Mechanistically, FBLN7 binds to the epidermal growth factor receptor (EGFR) through its EGF-like domain, together with the EGF-like calcium-binding domain, and induces EGFR autophosphorylation at tyrosine (Y) 1068 and Y1173, which activates downstream focal adhesion kinase/AKT signaling, thereby leading to fibroblast-to-myofibroblast transdifferentiation."
37344348
sparser
"We show that in highly motile GIV-wt cells with an intact GEF motif, EGFR autophosphorylation at Y992, Y1045, and Y1068 and the corresponding adaptor recruitment (PLCγ, cCbl, and Grb2) are enhanced, whereas in rapidly proliferating, GEF-deficient GIV-FA cells these events are suppressed."
20462955
sparser
"These combined data indicate that in lung epithelial cells, PTPµ selectively restrains EGFR autophosphorylation of Y845, Y992, Y1068, and Y1086."
18952113
sparser
"By contrast, proproliferative GIV-FA cells showed attenuated EGFR autophosphorylation at Y992, Y1045, and Y1068 but sustained phosphorylation at Y845 ( xref , B and C; and Supplemental Figure S3B)."
20462955
sparser
"Treatment of pancreatic carcinoma cells with purified EFEMP1 activates autophosphorylation of EGFR at the positions Tyr-992 and Tyr-1068, but not at the position Tyr-1048."
19804359
sparser
"A previous study has been shown that PRMT5 regulates the autophosphorylation of EGFR at Tyr1068 and Tyr1172 to promotes EMT in pancreatic cancer cells [ xref ]."
33495409
sparser
"Promigratory GIV-wt cells displayed enhanced EGFR autophosphorylation at Y992, Y1045, and Y1068, whereas Src-dependent phosphorylation at Y845 was similar to controls ( xref , B and C; and Supplemental Figure S3B)."
20462955
sparser
"However, ibrutinib only exhibited inhibitory activities upon 72 h continuous drug treatment. (Figure xref ) With 4 h pre-drug treatment in the H1975 cell line, WZ4002 (0.3 μM) completely suppressed EGFR Y1068 auto-phosphorylation within 24 h after drug removal."
27626175
sparser
"The results demonstrated that upon 4h's CHMFL-EGFR-26 treatment, the phosphorylation of EGFR Y1068 was inhibited with an EC 50 of 3.6 nM in BaF3-FL-EGFR-L858R -T790M cells and 50 nM in BaF3-TEL-EGFR cells (Figure xref ), suggesting that the drug shows 15-fold selectivity between the EGFR wt and EGFRL858R/T790M. Meanwhile, AZD9291 exhibited similar selectivity between the EGFR wt and EGFR L858R/T790M mutant Y1068 auto-phosphorylation (EC 50 : 63nM and 3.2 nM respectively)."
28407693
sparser
"EGFR autophosphorylation at Y1068 was also attenuated by NME1 overexpression."
31311812
trips
"NEU1 overexpression diminished EGF-stimulated EGFR Tyr-1068 autophosphorylation by up to 44% but enhanced MUC1-dependent Pseudomonas aeruginosa adhesion by 1.6-1.7-fold and flagellin-stimulated ERK1/2 activation by 1.7-1.9-fold."
22247545
sparser
"We found that PDGXs differ considerably from each other in the expression of total EGFR and autophosphorylated EGFR at tyrosine 1068 (p-EGFR Y1068) and 1173 (p-EGFR Y1173) ( xref )."
25598002
sparser
"In contrast, blockade or silencing of Src only suppressed EGF-induced EGFR phosphorylation at Y845 but did not affect EGFR autophosphorylation at Y1068 by EGF, indicating that ligand-dependent EGFR phosphorylation at Y845 depends on Src whereas autophosphorylation at other residues such as Y1068 is Src-independent."
23349873
sparser
"Moreover, previous study proved that PRMT5‐mediated EGFR Arg1175 methylation positively modulates EGF‐induced EGFR trans‐autophosphorylation at Tyr 1173, but have no effect on EGFR trans‐autophosphorylation at Tyr 1086, 845, 992, 1045 and 1148. xref Herein, we for the first time find that PRMT5 promotes the autophosphorylation of EGFR at Y1068 and Y1172 to activate Akt‐β‐catenin axis in pancreatic cancer cells."
31851779
sparser
"Autophosphorylation of EGFR on Tyr1068 leads to activation of Akt xref and since Akt is one of the major mediators of the antiapoptotic effects of EGFR activation xref , it is possible that Env engages this pathway to evade apoptosis."
23251519
sparser
"Taken together, our findings suggest that in cancer cells harboring elevated FAM83B expression, unphosphorylated EGFR and FAM83B complexes result in the increased steady-state autophosphorylation of EGFR on Tyrosine 1068."
23912460
sparser
"Hence, the low level of EGFR autophosphorylation on Y1068 in the presence of HER2 reduces the interaction of Grb2 directly and that of c-Cbl and Shc proteins indirectly."
23027125
sparser
"Depletion of NEU1 increases EGF-stimulated EGFR Tyr-1068 auto-phosphorylation and activation, decreases MUC1-mediated Pseudomonas aeruginosa adhesion and flagellin-stimulated ERK1/2 activation."
24337808
sparser
"The results demonstrated that ibrutinib potently inhibited both EGFR wt/mutant auto-phosphorylation at Y1068. (Fig. xref and xref ) In sensitive cell lines (H1975, PC-9, H3255, HCC827) it also potently inhibited targets downstream of ERK. (Fig. xref and xref ) Interestingly, it significantly affected the phosphorylation of Akt Thr308 and Ser473 in H1975 cells, however not the other three mutant EGFR-expressing cell lines. (Fig. xref , xref ) Although it did not have apparent enzymatic activity against PI3K kinase. ( xref ) This is different from other reported third generation EGFR inhibitors (WZ4002, CO1686 and AZD9291) that can significantly affect the phosphorylation of AktS473 in L858R and del 19 mutant cancer cell lines."
26375053