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EGFR phosphorylates itself on Y1016. 39 / 39
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"The results obtained suggest that the autophosphorylation of EGFR at tyrosine 992 is required for EGF-induced membrane ruffle formation and that the docking enzyme PLCγ1 mediates the signaling from th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12586341
sparser
"We show that in highly motile GIV-wt cells with an intact GEF motif, EGFR autophosphorylation at Y992, Y1045, and Y1068 and the corresponding adaptor recruitment (PLCγ, cCbl, and Grb2) are enhanced, whereas in rapidly proliferating, GEF-deficient GIV-FA cells these events are suppressed."
20462955
sparser
"We evaluated the tandem SH2 domains from wild-type phospholipase C γ1 (PLCγ1) fused to enhanced green fluorescent protein (EGFP), hereafter denoted as tSH2-WT, as a biosensor for studying the dynamics of EGFR autophosphorylation at Tyr 992 ."
31164479
sparser
"These results suggest that autophosphorylation of EGFR at tyrosine 992 is indispensable for EGF-induced membrane ruffle formation."
12586341
sparser
"Treatment of pancreatic carcinoma cells with purified EFEMP1 activates autophosphorylation of EGFR at the positions Tyr-992 and Tyr-1068, but not at the position Tyr-1048."
19804359
sparser
"In pancreatic adenocarcinomas, Fibulin-3 binds EGFR (competitive to EGF) causing autophosphorylation of EGFR at Tyr-992 and Tyr-1068 and the subsequent phosphorylation of AKT at Thr-308 and ERK at Thr-202 and Tyr-204 and, thus, accelerates pancreatic adenocarcinoma growth xref ."
23936443
sparser
"Moreover, myricetin has been found to increase EGF-induced autophosphorylation of epidermal growth factor receptor (EGFR) at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173, as well as inhibit the autop[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26455550
sparser
"It was revealed that Cu(edtc) 2 enhanced EGFR autophosphorylation (at approximately 135 kDa) at the site of Y992 but not Y1068 at 0.5 h after treatment with Cu(edtc) 2 ( xref A–C)."
30352976
rlimsp
"EGFR has an extracellular ligand-binding domain, a membrane-spanning domain and a cytoplasmatic protein tyrosine kinase domain with a carboxyl terminal that contains tyrosine residues that undergo autophosphorylation during receptor activation [31-34]. Three major tyrosine sites, Y1068, Y1173 and Y1148 and two minor tyrosine sites, Y992 and Y1086, serve as sites of autophosphorylation following ligand binding or transphosphorylation by other stimuli (figure 2)."
18460189
rlimsp
"GIV-wt cells showed increased EGFR autophosphorylation at Y992 at both 5 and 15 min and at Y1045 and Y1068 at 15 min, whereas GIV-FA cells showed sustained phosphorylation at Y845 at 15 and 30 min."
20462955
sparser
"Importantly, blockade of EGFR kinase activity by pretreatment with erlotinib, as visualized by reduced auto-phosphorylation of nEGFR at Y992, reduced AGO2 phosphorylation at Y393 in response to irradiation ( xref )."
29253018
sparser
"These combined data indicate that in lung epithelial cells, PTPµ selectively restrains EGFR autophosphorylation of Y845, Y992, Y1068, and Y1086."
18952113
rlimsp
"Furthermore, we also observed increased phosphorylation of EGFR at its autophosphorylation tyrosine sites Y992, Y1045, and Y1068, providing further evidence supporting enhanced mutant receptor activation (Fig. 3B)."
27900366
sparser
"It is worthy of notice that myricetin increased EGF-induced the autophosphorylation of EGFR at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173 ( Fig. 3 C, lane 3) although myricetin inhibited the autoph[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18995957
rlimsp
"Substantial inhibition (about 90%) of rasGAP tyrosine phosphorylation by the EGF receptor occurred only when the remaining autophosphorylation site (Tyr-992) was mutated, in the context of this truncated receptor or in the full-length receptor mutated at all four other autophosphorylation sites."
8408058
sparser
"More importantly, EFEMP1 has been shown to bind EGFR in a competitive manner relative to EGF to autophosphorylate EGFR at the positions Tyr-992 and Tyr-1068 for further activation of AKT [20] ."
21163514
rlimsp
"IR caused EGFR tyrosine phosphorylation, whereas it did not induce EGFR autophosphorylation at Tyr992, Tyr1045, and Tyr1068 or Src-dependent EGFR phosphorylation at Tyr845."
16414009
rlimsp
"We show that in highly motile GIV-wt cells with an intact GEF motif, EGFR autophosphorylation at Y992, Y1045, and Y1068 and the corresponding adaptor recruitment (PLCγ, cCbl, and Grb2) are enhanced, whereas in rapidly proliferating, GEF-deficient GIV-FA cells these events are suppressed."
20462955
sparser
"Overexpression of PTP μ in EGF-treated cells decreased EGFR autophosphorylation of both Y992 (58% reduction) ( xref , lane 4 vs 3) and Y1068 (42% reduction) ( xref , lane 4 vs 3)."
21649524
rlimsp
"It was revealed that Cu(edtc)2 enhanced EGFR autophosphorylation (at approximately 135 kDa) at the site of Y992 but not Y1068 at 0.5 h after treatment with Cu(edtc)2 (Figure 8A–C)."
30352976
rlimsp
"The EGF can induce phosphorylation of all five tyrosine phosphorylation sites of EGFR (Peng et al., 2010). Among these sites, Y992, Y1173, and Y1045 are autophosphorylation sites, with Y1173 as a major one and Y992 as a minor one."
26442853
rlimsp
"Serum starved GIV-wt, GIV-FA, and control HeLa cells were stimulated with EGF, and EGFR autophosphorylation was assessed at Y992, Y1045, and Y1068, (docking sites of the SH2 adaptors, PLCγ1, cCbl, and Grb2) and at Y845 (the substrate for c-Src kinase that triggers mitosis; Tice ), using site-specific phospho-Tyr antibodies (Figure 2A)."
20462955
rlimsp
"Promigratory GIV-wt cells displayed enhanced EGFR autophosphorylation at Y992, Y1045, and Y1068, whereas Src-dependent phosphorylation at Y845 was similar to controls (Figure 2, B and C; and Supplemental Figure S3B)."
20462955
rlimsp
"In addition, the conversion of Tyr992, which is an EGFr ligand-induced autophosphorylation site, to phenylalanine also increases the rate of receptor internalization. However, the mutation of Tyr992 to a glutamate residue does not alter the receptor internalization rate. In addition, the truncation of the EGFr at glutamate 996 reduces the internalization rate by half. This result confirms previous reports that residues immediately C-terminal to Glu996 are necessary to allow the normal rate of ligand-induced receptor endocytosis. The data suggest that negative charge in the vicinity of Tyr992, and potentially the phosphorylation of the EGFr at Tyr992, reduces the rate of ligand-induced receptor endocytosis."
10413509
rlimsp
"By contrast, proproliferative GIV-FA cells showed attenuated EGFR autophosphorylation at Y992, Y1045, and Y1068 but sustained phosphorylation at Y845 (Figure 2, B and C; and Supplemental Figure S3B)."
20462955
rlimsp
"We designed oligopeptides consisting of amino-acid sequences of the major (Y1068, Y1148, and Y1173) and minor (Y992) autophosphorylation sites of EGFR."
16402038
rlimsp
"In pancreatic adenocarcinomas, Fibulin-3 binds EGFR (competitive to EGF) causing autophosphorylation of EGFR at Tyr-992 and Tyr-1068 and the subsequent phosphorylation of AKT at Thr-308 and ERK at Thr-202 and Tyr-204 and, thus, accelerates pancreatic adenocarcinoma growth [28]."
23936443
sparser
"EGFR autophosphorylation of Y992 provides a docking site for PLC γ [ xref , xref ] whose activation is a prerequisite to EGFR-mediated cell migration [ xref , xref ]."
21649524
rlimsp
"Requirement of autophosphorylated tyrosine 992 of EGF receptor and its docking protein phospholipase C gamma 1 for membrane ruffle formation."
12586341
rlimsp
"Requirement of autophosphorylated tyrosine 992 of EGF receptor and its docking protein phospholipase C gamma 1 for membrane ruffle formation. Stimulation of the epidermal growth factor receptor (EGFR) produces membrane ruffles through the small G protein Rac1; however, the signaling pathway from EGFR to Rac1 has not yet been clarified. Here, we show that autophosphorylation of EGFR at tyrosine 992 is required for EGF-induced membrane ruffle formation in CHO cells. Signaling from the autophosphorylated tyrosine 992 appears to be mediated by phospholipase C (PLC) gamma 1."
12586341
rlimsp
"Here, we show that autophosphorylation of EGFR at tyrosine 992 is required for EGF-induced membrane ruffle formation in CHO cells."
12586341
rlimsp
"These results, taken together, suggest that autophosphorylation of EGFR at tyrosine 992 and the subsequent PLC gamma 1 activation transduce the signal to Rac1 to induce membrane ruffle formation."
12586341
sparser
"By contrast, proproliferative GIV-FA cells showed attenuated EGFR autophosphorylation at Y992, Y1045, and Y1068 but sustained phosphorylation at Y845 ( xref , B and C; and Supplemental Figure S3B)."
20462955
rlimsp
"Ba/F3 cells expressing either L858R or P848L mutation were treated for 1 h. (D, E) and 24 h. (F, G) with the indicated concentrations of JAKi's AZD1480 and ruxolitinib, and autophosphorylations of EGFR at Tyr992, p‐STAT5 at Tyr 694, and p‐ERK were measured by western blotting."
31314158
rlimsp
"Both TK domain (Y847) as well as autophosphorylation sites (Y992, Y1068) of EGFR are affected more, with concurrent inhibition of Erk1/2 and Akt phosphorylation."
19240716
sparser
"Myricetin inhibited the autophosphorylation of endogenous EGFR at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173 ( Fig. 3 C, lane 4)."
18995957
sparser
"Promigratory GIV-wt cells displayed enhanced EGFR autophosphorylation at Y992, Y1045, and Y1068, whereas Src-dependent phosphorylation at Y845 was similar to controls ( xref , B and C; and Supplemental Figure S3B)."
20462955
rlimsp
"In the case of the EGF receptor (EGFR), the RTK activity is stimulated by ligand binding, resulting in autophosphorylation of specific tyrosine residues (Tyr992 and Tyr1148), which are located within the COOH-terminal intracellular domain of the receptor [10]."
18253097
sparser
"Of note, at the concentration used (5 μM), AG1478 did not significantly affect viability of MDA-MB-231 cells, though serum-induced EGFR auto-phosphorylation at Tyr-992 and Tyr-1068 was markedly inhibi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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