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EGFR phosphorylates itself on Y1069. 25 / 25
| 1 13 11
sparser
"Mechanistically, we demonstrated that rLZ-8 bound to EGFR to induce EGFR autophosphorylation at tyrosine1045 and trigger ubiquitination by inducing the formation of EGFR/Cbl complexes, resulting in the degradation of EGFR; however, Cbl-shRNA abolished rLZ-8-induced EGFR degradation."
28198003
sparser
"It was notable that IGF1R knockdown influenced not only EGFR autophosphorylation on Y1045, 1068, 1086, 1145 and 1173, but also the phosphorylation of Y845, a Src-mediated transphosphorylation event ."
17320820
rlimsp
"In controls, autophosphorylation of EGFR at both pY1068 and pY1045 peaked at 5 min but was reduced by 15 min after EGF stimulation (Figure 3A), whereas in Gαs-depleted cells EGFR autophosphorylation at these sites was increased greater than threefold at both 5 and 15 min after ligand stimulation (Figure 3, A and B)."
23051738
sparser
"Moreover, myricetin has been found to increase EGF-induced autophosphorylation of epidermal growth factor receptor (EGFR) at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173, as well as inhibit the autop[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26455550
rlimsp
"To confirm the role of Src-dependent phosphorylation of EGFR, we mutated the Src-dependent phosphorylation site, Y845F, or the autophosphorylation site, Y1045F, and expressed these mutations in cos-7 cells after EGFR knock down."
28459445
sparser
"We show that in highly motile GIV-wt cells with an intact GEF motif, EGFR autophosphorylation at Y992, Y1045, and Y1068 and the corresponding adaptor recruitment (PLCγ, cCbl, and Grb2) are enhanced, whereas in rapidly proliferating, GEF-deficient GIV-FA cells these events are suppressed."
20462955
sparser
"It is worthy of notice that myricetin increased EGF-induced the autophosphorylation of EGFR at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173 ( Fig. 3 C, lane 3) although myricetin inhibited the autoph[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18995957
rlimsp
"However, gefitinib induced a more marked decrease in EGFR autophosphorylation at tyrosine residues 1173, 845, and 1045 and a lesser decrease at Y992 in del746–750 cells, compared with the autophosphorylation levels in L858R cells."
28352061
rlimsp
"We found a new mechanism of action, in that T315 appears to directly bind EGFR and triggers EGFR-Y1045 autophosphorylation, whereby its degradation is triggered through the ubiquitin-proteasome pathway."
26583948
sparser
"Fig 5C: Similar to 2G, EGFR autophosphorylation on Y1068 and Y1045 should also be measured, as well as downstream activation of p42/44 ERK MAPK?"
39937579
rlimsp
"ERK(1/2) phosphorylation by Src activation depended on β-arrestin 2, but not β-arrestin 1, was accompanied by Src/EGFR co-precipitation and phosphorylation of the EGFR at the Src-phosphorylated Y845 site and the Y1045 autophosphorylation site; it was independent of transactivation but dependent on MAPK/ERK kinase activity, suggesting EGFR phosphorylation independently of the receptor-tyrosine kinase or activation of Ras or Raf directly from Src."
20831657
rlimsp
"For adaptation to EGFR signaling, c-Cbl binds to autophosphorylated Tyr1045 through its SH2 domain, mediating EGFR ubiquitination and the subsequent desensitization of the proliferating signal by internalization of the active EGFR complex into endosome (Grovdal et al., 2004)."
31722512
sparser
"Following the Reviewer’s request, we have now carried out new experiments to assess the EGFR autophosphorylation on Y1068 and Y1045, as well as downstream activation of p42/44 ERK MAPK."
39937579
sparser
"rLZ-8 could bind to EGFR to induce EGFR autophosphorylation at tyrosine-1045 and trigger ubiquitination by inducing the formation of EGFR/Cbl complexes, subsequently leading to EGFR protein degradation."
40733125
rlimsp
"gefitinib induced a more marked decrease in EGFR autophosphorylation at tyrosine residues 1173, 845, and 1045 and a lesser decrease at Y992 in del746–750 cells, compared with the autophosphorylation levels in L858R cells."
29447182
rlimsp
"Upon binding of the ligand to EGFR, the tyrosine 1045 site on the cytoplasmic domain of the EGFR becomes autophosphorylated."
28410196
trips
"Mechanistically, we demonstrated that rLZ-8 bound to EGFR to induce EGFR autophosphorylation at tyrosine1045 and trigger ubiquitination by inducing the formation of EGFR/Cbl complexes, resulting in the degradation of EGFR; however, Cbl-shRNA abolished rLZ-8-induced EGFR degradation."
28198003
rlimsp
"Y1045 is the major autophosphorylation residue of EGFR and is involved in c-cbl–dependent EGFR ubiquitination and internalization (Sorkin and Goh, 2008)."
22438585
rlimsp
"Mechanistically, we demonstrated that rLZ-8 bound to EGFR to induce EGFR autophosphorylation at tyrosine1045 and trigger ubiquitination by inducing the formation of EGFR/Cbl complexes, resulting in the degradation of EGFR; however, Cbl-shRNA abolished rLZ-8-induced EGFR degradation."
28198003
rlimsp
"We have demonstrated autophosphorylation of Tyr1045, 1068, 1148, 1173 as well as phosphorylation of Tyr845 of EGF receptor in response to H2O2, as assessed by autophosphorylation specific antibody."
20586269
sparser
"Five minutes after EGF addition, EGFR auto-phosphorylation on Tyr 1068 and Tyr 1045 was 2.7- and 1.8-fold greater, respectively, in SEPW1-overexpressing cells compared to empty vector-transfected cont[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25721765
sparser
"Myricetin inhibited the autophosphorylation of endogenous EGFR at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173 ( Fig. 3 C, lane 4)."
18995957
sparser
"Although further work is needed to define the precise molecular mechanisms of EGFR internalization and degradation, the accumulated evidence suggests the following model of EGF-induced EGFR endocytosis: EGFR that is autophosphorylated at Y1045 within its C-terminal domain recruits and interacts with the TKB domain of c-Cbl."
19489726
sparser
"Promigratory GIV-wt cells displayed enhanced EGFR autophosphorylation at Y992, Y1045, and Y1068, whereas Src-dependent phosphorylation at Y845 was similar to controls ( xref , B and C; and Supplemental Figure S3B)."
20462955
sparser
"By contrast, proproliferative GIV-FA cells showed attenuated EGFR autophosphorylation at Y992, Y1045, and Y1068 but sustained phosphorylation at Y845 ( xref , B and C; and Supplemental Figure S3B)."
20462955