IndraLab

Statements

EGFR phosphorylates itself on Y869. 26 / 26
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rlimsp
"Taken together, there are three mechanisms by which T. gondii prevents targeting by autophagosomes; two that are triggered during invasion of the host cell: (a) MIC3/MIC6‐dependent EGFR autophosphorylation resulting in Akt activation (Muniz‐Feliciano et al., 2013), (b) FAK‐mediated activation of Src at the level of the moving junction leading to Src‐mediated transactivation of EGFR (Y845 phosphorylation) and STAT3 signalling (Portillo et al., 2017), and (c) a third mechanism that operates at later stages of the intracellular stage of T. gondii whereby prolonged PKCα/PKCβ‐Src signalling maintains EGFR autophosphorylation that in turn appears to function via Akt (Figure 9)."
31290228
rlimsp
"LPA equally protected B82L cells, or transfectants expressing EGFR, the kinase dead EGFR(K721A), EGFR(Y5F) receptor mutant, which lacks five autophosphorylation sites, or EGFR(Y845F), which lacks the Src phosphorylation site from tumor necrosis factor-alpha-induced apoptosis."
15364923
rlimsp
"A previous study indicates that EGFR Y845 phosphorylation by Src has no effect on the autophosphorylation of EGFR [87]."
26771606
sparser
"Moreover, myricetin has been found to increase EGF-induced autophosphorylation of epidermal growth factor receptor (EGFR) at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173, as well as inhibit the autop[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26455550
rlimsp
"Notably, Y1173 and Y845 of EGFR are the major sites of EGFR autophosphorylation which occurs as a result of EGF binding [98]."
21637383
rlimsp
"On the other hand, several reports demonstrated that Y845 is not EGFR autophosphorylation site, instead EGFR phosphorylation at Y845 is regulated by the SRC kinase [18–20]."
25301722
rlimsp
"Furthermore, the association of Src with Shc, Grb2 and the EGFR after angiotensin II stimulation [25], together with previously described Src-mediated phosphorylation of tyrosine residues Y845 and Y1101 of the EGFR [35,36], suggest an additional means of receptor activation utilized by GPCRs that is distinct from EGFR autophosphorylation."
11250708
rlimsp
"Addition of galardin to cells stimulated with bombesin or LPA specifically inhibited total EGFR phosphorylation, as well as site-specific phosphorylation of tyrosine 845, a putative Src phosphorylation site, and tyrosine 1068, a typical autophosphorylation site."
14568001
sparser
"It is worthy of notice that myricetin increased EGF-induced the autophosphorylation of EGFR at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173 ( Fig. 3 C, lane 3) although myricetin inhibited the autoph[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18995957
rlimsp
"Although EGFR is generally activated through ligand binding and autophosphorylation of its cytoplasmic tail, it is well established that Src non-receptor TK, one of the EGFR downstream transducers, can transactivate EGFR by phosphorylating tyrosine 845 (Y845); this event may contribute to full receptor activation [13]."
26325669
rlimsp
"Phosphorylation of EGFR on Y845 is usually catalyzed by Src and other Src family PTKs, and in some cases, is mediated by a non-Src family PTK or autophosphorylation of EGFR."
23702846
rlimsp
"It caused EGFR down-regulation and inhibited EGFR Tyr-845 autophosphorylation in both Du145 and PC-3 cells."
20651333
rlimsp
"These results suggest that EGFR has the potential to autophosphorylate on Y845 in an EGFR conformation-dependent manner."
23702846
rlimsp
"This idea is also supported by the study of Qiu et al. [192], who showed that a mutant EGFR, in which a stop codon had been introduced following the position encoding G998, could autophosphorylate Y845 in a PP2-insensitive and erlotinib-sensitive manner."
23702846
rlimsp
"Src dependence of EGFR Y845 phosphorylation is well established (Biscardi ; Bromann ; Ishizawar and Parsons, 2004). However, it has been suggested that phosphorylation of Y845 on one of NSCLC-associated EGFR mutants, L861Q, represents an autophosphorylation site and is Src activity-independent based on the use of EGFR and Src inhibitors (Yang ). However, Y845 phosphorylation in EGFR L858R-expressing cells was still sensitive to Src inhibitors (Yang ). While it is reasonable that activated mutant EGFRs might acquire the ability to autophosphorylate Y845, constitutive activity of Src and the enhanced EGFR-Src interaction in mutant EGFR-expressing cells suggest that phosphorylation of Y845 is quite likely to be predominantly Src-dependent."
19305428
sparser
"These combined data indicate that in lung epithelial cells, PTPµ selectively restrains EGFR autophosphorylation of Y845, Y992, Y1068, and Y1086."
18952113
rlimsp
"EGFR is a tyrosine kinase receptor which is activated by autophosphorylation at Tyr845 residue upon ligand binding [18] and we examined whether the activity was altered by de-N-glycosylation."
24932134
sparser
"It caused EGFR down-regulation and inhibited EGFR Tyr-845 autophosphorylation in both Du145 and PC-3 cells."
20651333
rlimsp
"Transphosphorylation of EGFR at Y845 plays an important role in its autophosphorylation and kinase activity."
24677053
rlimsp
"These collective findings indicate that EGFR transactivation by ATP is critically dependent on initial activation of Src, which in turn phosphorylates EGFR at Y845 and activates ADAM17 to promote ligand-dependent EGFR autophosphorylation."
23349873
rlimsp
"HEK293 cells co-expressing EGFR and E-cadherin displayed EGFR phosphorylation but not in cells expressing EGFR alone upon 30 min seeding on culture dish (Supplemental Figure 4C). On further analysis, we observed that residues Y845, Y1068, Y1148 and Y1173 were readily autophosphorylated, while residue Y1086 phosphorylation was barely detectable (Figure 5D)."
19935697
rlimsp
"We show that EGFR is capable of direct autophosphorylation of tyrosine 845, which is located on its kinase activation loop, and that the kinase activity of EGFR is approximately 500-fold higher in the presence of EGF vs the inhibitory anti-EGFR antibody cetuximab."
19518076
sparser
"Myricetin inhibited the autophosphorylation of endogenous EGFR at Tyr845, Tyr992, Tyr1045, Tyr1068, and Tyr1173 ( Fig. 3 C, lane 4)."
18995957
rlimsp
"Expression of kinase domain of EGFR(L858R) and EGFR(L861Q) exhibited auto-phosphorylation of Y869; this was inhibited by EGFR TKIs but not by Src kinase inhibitor."
17927978
sparser
"We show that EGFR is capable of direct autophosphorylation of tyrosine 845, which is located on its kinase activation loop, and that the kinase activity of EGFR is ~500-fold higher in the presence of EGF vs. the inhibitory anti-EGFR antibody Cetuximab."
19518076
rlimsp
"In contrast, Tyr 845 in the EGF receptor has never been identified as an autophosphorylation site."
11250711