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JOSD1 affects MCL1
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JOSD1 binds MCL1.
3 | 8 9
MCL1 binds JOSD1. 10 / 20
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"As shown in Fig. xref , the interaction between JOSD1 and MCL-1 was strikingly disrupted in TRAF4-deficient cells, while other deubiquitinases were not affected."
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"Likewise, compared with CAL27R-sgCtrl cells, the depletion of TRAF4 destroyed the interaction between JOSD1 and MCL-1, which was further enhanced in the presence of radiation (Fig. xref )."
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"MCL1 WT and S159D mutant (which is a phosphomimetic mutant in the GSK3β phosphorylation site), but not MCL1 S159A mutant (which abolished phosphorylation by GSK-3), substantially decreased the interaction between JOSD1 and MCL-1 in the presence of IR in CAL27R cells (Fig. xref )."
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"TRAF4 promoted Akt K63-linked ubiquitination and activation to suppress GSK3β activity and MCL-1 S159 phosphorylation, facilitating the interaction between MCL-1 and JOSD1, which ultimately promoted the stability and expression of MCL-1 to confer resistance to radiotherapy in OSCC cells (Fig. 7G)."
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"These results suggest that the phosphorylation of MCL-1 S159 disrupts the interaction between JOSD1 and MCL-1, and eventually induces MCL-1 ubiquitination and degradation in TRAF4-knockout OSCC cells."
36535926
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"These results indicate that depletion of TRAF4 sensitizes OSCC cells to radiotherapy, which depends on disrupting the interaction of JOSD1 with MCL-1 to promote MCL-1 ubiquitination and degradation."
36535926
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"Our results indicated that depletion of TRAF4 disrupted the interaction between MCL-1 and JOSD1, particularly under IR treatment."
36535926
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"TRAF4 promoted Akt K63-linked ubiquitination and activation to suppress GSK3β activity and MCL-1 S159 phosphorylation, facilitating the interaction between MCL-1 and JOSD1, which ultimately promoted the stability and expression of MCL-1 to confer resistance to radiotherapy in OSCC cells (Fig. xref )."
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"Further research showed that curcumol-induced Mcl-1 Ser159 phosphorylation is required to disrupt the interaction between deubiquitinase JOSD1 and Mcl-1 and eventually induce Mcl-1 ubiquitination and degradation."
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"Likewise, compared with CAL27R-sgCtrl cells, the depletion of TRAF4 destroyed the interaction between JOSD1 and MCL-1, which was further enhanced in the presence of radiation (Fig. 5B)."
36535926
JOSD1 deubiquitinates MCL1.
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JOSD1 deubiquitinates MCL1. 1 / 2
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"JOSD1 could also deubiquitinate MCL1 and stabilise MCL1 to suppress mitochondrial apoptosis and confer chemoresistance in gynaecological cancer [20]."
39284830
JOSD1 activates MCL1.
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JOSD1 activates MCL1. 2 / 2
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"JOSD1 promotes chemoresistance by stabilizing MCL1 in gynecologic tumors [40]."
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"JOSD1 overexpression caused chemoresistance in gynaecological cancer by upregulating the MCL1 protein."
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JOSD1 ubiquitinates MCL1.
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JOSD1 leads to the ubiquitination of MCL1. 1 / 1
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"Moreover, the ubiquitination assay showed that ectopic overexpression of JOSD1 remarkably abolished the IR-induced ubiquitination of MCL-1 in CAL27R cells (Fig. 5C)."
36535926
JOSD1 inhibits MCL1.
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JOSD1 inhibits MCL1. 1 / 1
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"By stabilizing MCL1, JOSD1 inhibits the mitochondrial apoptosis pathway and exerts antiapoptotic effects."
35924961
JOSD1 increases the amount of MCL1.
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JOSD1 increases the amount of MCL1. 1 / 1
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"High JOSD1 protein level causes the chemoresistance in gynecological cancer by removing the K48-linked polyubiquitin and upregulating the MCL1 protein level, and is negatively correlated with prognosi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33508399
JOSD1 affects YAP1
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JOSD1 binds YAP1.
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YAP1 binds JOSD1. 9 / 9
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"We conducted deletion construct experiments to study the interaction between YAP and JOSD1."
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"JOSD1 is associated with YAP and regulates YAP protein stability."
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"In addition, endogenous immunoprecipitation experiments demonstrated that JOSD1 could interact with YAP in colon cancer cells (Fig. xref )."
39143074
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"We conducted deletion construct experiments to study the interaction between YAP and JOSD1."
39143074
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"In addition, JOSD1 interacts with YAP through its N terminus (Fig. xref )."
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"Furthermore, subsequent experiments involving the depletion of YAP in colon cancer cells demonstrated a significant reduction in YAP binding to the JOSD1 promoter region, as evidenced by the ChIP assay results (Fig. xref )."
39143074
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"ChIP assays have shown that YAP binds to JOSD1’s promoter region, promoting its gene transcription."
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"ChIP assays have shown that YAP binds to JOSD1's promoter region, promoting its gene transcription."
39143074
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"After demonstrating the association between JOSD1 and YAP, we investigated its biological effect on YAP protein."
39143074
JOSD1 decreases the amount of YAP1.
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JOSD1 decreases the amount of YAP1. 3 / 3
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"We inhibited YAP degradation using the proteasome inhibitor MG132 and found that removal of JOSD1 decreased YAP protein levels in colon cancer cells."
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"The results from these experiments confirmed that JOSD1 inhibited the total ubiquitination level of YAP, particularly the K48-linked ubiquitination level (Fig. 6A–C)."
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"The RNA sequencing data demonstrated that JOSD1 silencing induced dramatic change of Hippo signature (Fig. 1C), while JOSD1 depletion decreased the global YAP target gene expression from GSEA analysis (NES = 1.65; P < 0.001; Fig. 1D)."
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JOSD1 increases the amount of YAP1.
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JOSD1 increases the amount of YAP1. 2 / 2
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"In the gene expression assay, JOSD1 wild type form could increase YAP protein level, while the JOSD1 could not (Fig. 3K)."
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"The RNA sequencing data demonstrated that JOSD1 silencing induced dramatic change of Hippo signature (Fig. 1C), while JOSD1 depletion decreased the global YAP target gene expression from GSEA analysis (NES = 1.65; P < 0.001; Fig. 1D)."
39143074
JOSD1 deubiquitinates YAP1.
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Mutated JOSD1 leads to the deubiquitination of YAP1. 1 / 1
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"Notably, our findings demonstrated that the mutant JOSD1 attenuates the impact of JOSD1 on YAP ubiquitination (Fig. 6G, H)."
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JOSD1 deubiquitinates YAP1. 1 / 1
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"The structural domain-based ubiquitination assays showed that the N-terminal structural domain of JOSD1 is crucial for the deubiquitination of YAP by JOSD1 (Fig. 6I, J)."
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JOSD1 activates YAP1.
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JOSD1 activates YAP1. 2 / 2
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"JOSD1 activates Hippo/YAP axis in colon cancer cells."
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"The results indicated that wild-type JOSD1 increased the stability of YAP, while catalytically defective JOSD1 did not (Fig. 5F, G)."
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JOSD1 affects SOCS1
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JOSD1 binds SOCS1.
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JOSD1 binds SOCS1. 7 / 8
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"Co-immunoprecipitation assay further shows that endogenous SOCS1 interacts with ectopically expressed JOSD1 both in HepG2 and primary mouse hepatocytes (Fig. xref )."
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"Also, the functional role of the JOSD1–SOCS1 axis in human subjects is to be investigated."
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"In Duck Tembesu virus infection, JOSD1 interacts with SOCS1 via its SH2 domain and stabilises SOCS1."
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"JOSD1 interacts with and deubiquitinates SOCS1 to mitigate apoptosis under proteotoxic stress."
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"JOSD1 physically interacts with SOCS1, which is an essential negative regulator of many cytokines signaling, and enhances SOCS1 stability by deubiquitinating K48-linked polyubiquitination of SOCS1."
28355105
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"In Duck Tembesu virus infection, JOSD1 interacts with SOCS1 via its SH2 domain and stabilises SOCS1."
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"JOSD1 physically interacts with SOCS1, which is an essential negative regulator of many cytokines signaling, and enhances SOCS1 stability by deubiquitinating K48 linked polyubiquitination of SOCS1."
28355105
JOSD1 deubiquitinates SOCS1.
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JOSD1 deubiquitinates SOCS1. 2 / 3
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"JOSD1 interacts with and deubiquitinates SOCS1 to mitigate apoptosis under proteotoxic stress."
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"Since JOSD1 deubiquitinates and stabilises SOCS1, we next examined whether SOCS1 alone could impede proteotoxicity in hepatocytes."
39284830
JOSD1 ubiquitinates SOCS1.
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JOSD1 leads to the ubiquitination of SOCS1. 1 / 1
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"JOSD1 Negatively Regulates Type-I Interferon Antiviral Activity by Deubiquitinating and Stabilizing SOCS1."
28355105
JOSD1 inhibits SOCS1.
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JOSD1 inhibits SOCS1. 1 / 1
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"JOSD1 can inhibit the IFN-1-induced signalling pathway and antiviral response by physically interacting with SOCS1 and enhancing SOCS1 stability by cleaving K48-ubiquitin chains from SOCS1 [21]."
39284830
JOSD1 activates SOCS1.
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JOSD1 activates SOCS1. 1 / 1
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"Moreover, JOSD1 could enhance the half-life of SOCS1 and protect it from cycloheximide-mediated decrease with time in HepG2 cells under proteotoxic stress (Fig. 4F)."
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YAP1 affects JOSD1
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YAP1 binds JOSD1.
| 3 6
YAP1 binds JOSD1. 9 / 9
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"We conducted deletion construct experiments to study the interaction between YAP and JOSD1."
39143074
sparser
"JOSD1 is associated with YAP and regulates YAP protein stability."
39143074
sparser
"In addition, endogenous immunoprecipitation experiments demonstrated that JOSD1 could interact with YAP in colon cancer cells (Fig. xref )."
39143074
sparser
"We conducted deletion construct experiments to study the interaction between YAP and JOSD1."
39143074
sparser
"In addition, JOSD1 interacts with YAP through its N terminus (Fig. xref )."
39143074
sparser
"Furthermore, subsequent experiments involving the depletion of YAP in colon cancer cells demonstrated a significant reduction in YAP binding to the JOSD1 promoter region, as evidenced by the ChIP assay results (Fig. xref )."
39143074
sparser
"ChIP assays have shown that YAP binds to JOSD1’s promoter region, promoting its gene transcription."
39143074
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"ChIP assays have shown that YAP binds to JOSD1's promoter region, promoting its gene transcription."
39143074
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"After demonstrating the association between JOSD1 and YAP, we investigated its biological effect on YAP protein."
39143074
YAP1 increases the amount of JOSD1.
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YAP1 increases the amount of JOSD1. 1 / 1
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"Moreover, the study revealed that YAP upregulates JOSD1 expression, indicating a mutual reinforcement between JOSD1 and Hippo signaling in a positive feedback loop (Fig. 8)."
39143074
YAP1 activates JOSD1.
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YAP1 activates JOSD1. 1 / 1
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"Conversely, JOSD1 mRNA and protein levels in HCT116 and SW480 cells were increased by activation of YAP by XMU-MP-1 (Fig. 7M–P)."
39143074
SOCS1 affects JOSD1
1 | 5 4
SOCS1 binds JOSD1.
1 | 3 4
JOSD1 binds SOCS1. 7 / 8
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"Co-immunoprecipitation assay further shows that endogenous SOCS1 interacts with ectopically expressed JOSD1 both in HepG2 and primary mouse hepatocytes (Fig. xref )."
39284830
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"Also, the functional role of the JOSD1–SOCS1 axis in human subjects is to be investigated."
39284830
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"In Duck Tembesu virus infection, JOSD1 interacts with SOCS1 via its SH2 domain and stabilises SOCS1."
39284830
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"JOSD1 interacts with and deubiquitinates SOCS1 to mitigate apoptosis under proteotoxic stress."
39284830
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"JOSD1 physically interacts with SOCS1, which is an essential negative regulator of many cytokines signaling, and enhances SOCS1 stability by deubiquitinating K48-linked polyubiquitination of SOCS1."
28355105
sparser
"In Duck Tembesu virus infection, JOSD1 interacts with SOCS1 via its SH2 domain and stabilises SOCS1."
39284830
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"JOSD1 physically interacts with SOCS1, which is an essential negative regulator of many cytokines signaling, and enhances SOCS1 stability by deubiquitinating K48 linked polyubiquitination of SOCS1."
28355105
SOCS1 inhibits JOSD1.
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SOCS1 inhibits JOSD1. 2 / 2
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"The absence of SOCS1 attenuated JOSD1’s ability to reverse apoptosis under proteotoxic stress, while JOSD1 C36A cells were unaffected (Fig. 5C–E)."
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"Although overexpression of JOSD1 did not alter SOCS1 protein levels (Fig. 6B), we find a decline in SOCS1 following knockdown of JOSD1 (Fig. 6C)."
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JOSD1 affects apoptotic process
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JOSD1 inhibits apoptotic process.
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JOSD1 inhibits apoptotic process. 6 / 6
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"Using siRNA-mediated JOSD1 knockdown, we confirmed that the loss of JOSD1 could markedly increase the apoptosis of gynecological cancer cells ."
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"In addition, in the FACS analysis, JOSD1 depletion was found to elevate cell apoptosis in colon cancer cells."
39143074
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"By stabilizing MCL1, JOSD1 inhibits the mitochondrial apoptosis pathway and exerts antiapoptotic effects."
35924961
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"JOSD1 was reported to stabilize MCL1 and inhibit mitochondrial-dependent apoptosis, which subsequently promoted chemotherapy resistance in leukemia [31]."
39143074
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"JOSD1 suppresses mitochondrial apoptosis to induce the chemotherapy drug resistance in the gynaecological tumor by deubiquitinating and stabilizing MCL1 (Wu et al., 2020)."
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"Moreover, with the treatment of CDDP, JOSD1 knockdown could increase expression of apoptosis-related proteins and promote cell apoptosis rate."
34261480
JOSD1 activates apoptotic process.
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JOSD1 activates apoptotic process. 4 / 4
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"We found that ATXN3 inhibited apoptosis, while JOSD1 and JOSD2 promoted apoptosis, JOSD2 inhibited DNA damage response, JOSD1 and JOSD2 promoted EMT, and JOSD2 suppressed RTK and androgen pathways.Moreover, we used the cBioPortal database to explore the co-expression (Table 3) and mutual exclusion (Table 4) between individual members of the MJDs family."
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"JOSD1 depletion led to severe apoptosis in gynaecological cancer cells both in vivo and in vitro."
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"Since the absence of JOSD1 caused significant enhancement of apoptosis, we proceeded to address how JOSD1 confers protection against proteotoxic stress."
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"In contrast to the depletion of JOSD1, we observed that expression of JOSD1 caused a noticeable reduction in cellular apoptosis as evidenced by decreased expression of CC3 and cleaved PARP (Fig. 2A) and in the number of dead cells (Fig. 2B)."
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JOSD1 affects Neoplasms
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JOSD1 activates Neoplasms.
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JOSD1 activates Neoplasms. 4 / 4
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"Furthermore , we found the high expression of Ki67 in JOSD1-depleted xenografts , indicating that JOSD1 could accelerate tumor proliferation in HNSCC ."
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"Knockdown of JOSD1 attenuates tumor growth and improves chemosensitivity of HNSCC cells in vivo."
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"Furthermore, we found the high expression of Ki67 in JOSD1-depleted xenografts, indicating that JOSD1 could accelerate tumor proliferation in HNSCC."
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"Additionally, JOSD1 suppression inhibited the tumor growth and improved chemosensitivity against cisplatin in a xenograft mouse model."
35924961
JOSD1 inhibits Neoplasms.
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JOSD1 inhibits Neoplasms. 3 / 3
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"Furthermore, we found the high expression of Ki67 in JOSD1-depleted xenografts, indicating that JOSD1 could accelerate tumor proliferation in HNSCC."
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"Additionally, JOSD1 suppression inhibited the tumor growth and improved chemosensitivity in vivo."
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"In our study, JOSD1 knockdown could inhibit the growth and colony formation of TSCCA and SCC25 cells, and suppress the tumor growth in vivo."
34261480
JOSD1 is modified
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JOSD1 is ubiquitinated.
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JOSD1 is ubiquitinated. 4 / 4
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"Notably, JOSD1’s ubiquitination can regulate its catalytic activity, but this modification is not necessary for JOSD2 to exert its DUB function."
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"As ubiquitinated JOSD1 could localise to the plasma membrane to influence membrane dynamics, cell motility, and pinocytosis [ xref ], we sought to investigate if similar translocation and localisation of JOSD1 could be seen under proteasomal inhibition."
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"In agreement with array data, USP2c and USP21c removed ubiquitin from both substrates; PLP2 from only one (ubiquitylated JOSD1); and Ataxin3 showed no activity towards either ( Fig. 7 )."
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"The ubiquitination of JosD1 triggers its localization to the membrane from the cytoskeletal fraction [ xref ]."
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JOSD1 is methylated.
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JOSD1 is methylated. 2 / 2
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"However, JOSD1 methylation levels in HCC tissues was higher than that in corresponding normal tissues without statistic difference, in spite of histological grades, TP53 mutations, lymph node metastatic, genders, and years ( xref )."
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"Furthermore, we analyzed the correlations between the degree of methylation of ATXN3, JOSD1, and JOSD2 and the mRNA expression levels with the cBioPortal database."
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JOSD1 affects KRAS
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JOSD1 deubiquitinates KRAS.
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JOSD1 deubiquitinates KRAS. 2 / 2
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"However, neither JOSD1 wild-type nor its enzymatic mutant failed to decrease KRAS polyubiquitination levels (Fig. 2g)."
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"Moreover, our data have demonstrated that JOSD1 does not interact with or deubiquitinate KRAS and its depletion has minimal effects on KRAS-mutant CRC cell growth; the anti-tumor effects of HY041004 on KRAS-mutant CRC are primarily attributed to JOSD2 inhibition."
40240366
JOSD1 decreases the amount of KRAS.
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JOSD1 decreases the amount of KRAS. 2 / 2
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"Correspondingly, depletion of JOSD2, but not JOSD1, significantly downregulated KRAS levels (Fig. 1e and Supplementary Fig. 1b, c), indicating JOSD2 positively regulates KRAS abundance."
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"However, neither JOSD1 wild-type nor its enzymatic mutant failed to decrease KRAS polyubiquitination levels (Fig. 2g)."
40240366
JOSD1 ubiquitinates KRAS.
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JOSD1 leads to the ubiquitination of KRAS. 1 / 1
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"On this basis, overexpression of JOSD2, not JOSD1, dramatically decreased the polyubiquitin chains of KRAS promoted by LZTR1 and restored the LZTR1-mediated KRAS levels downregulation (Fig. 2k and Supplementary Fig. 2k, l), indicating that JOSD2 reversed the effect of LZTR1-mediated KRAS ubiquitination degradation."
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BRD4 affects JOSD1
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BRD4 increases the amount of JOSD1.
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BRD4 increases the amount of JOSD1. 2 / 2
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"Besides, we found that knockdown of BRD4 by shRNA, resembling the effect of JQ1, inhibited the expression of JOSD1 (Fig. 2e, f), while BRD4 overexpression significantly upregulated JOSD1 in HNSCC cells (Fig. 2g, h), suggesting that BRD4 affected JOSD1 expression at the transcriptional level."
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"Taken together, these evidences illustrate that BRD4, as an epigenetic modulator, enhances the expression of JOSD1 by facilitating its transcription activation."
34261480
BRD4 activates JOSD1.
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BRD4 activates JOSD1. 2 / 2
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"Taken together , these evidences illustrate that BRD4 , as an epigenetic modulator , enhances the expression of JOSD1 by facilitating its transcription activation ."
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"BRD4 inhibition downregulates JOSD1 in HNSCC ."
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BRD4 decreases the amount of JOSD1.
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BRD4 decreases the amount of JOSD1. 1 / 1
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"Besides, we found that knockdown of BRD4 by shRNA, resembling the effect of JQ1, inhibited the expression of JOSD1 (Fig. 2e, f), while BRD4 overexpression significantly upregulated JOSD1 in HNSCC cells (Fig. 2g, h), suggesting that BRD4 affected JOSD1 expression at the transcriptional level."
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JOSD1 activates cell population proliferation. 3 / 3
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"Furthermore, JOSD1 silencing could inhibit proliferation and enhance chemosensitivity of HNSCC cells in vitro and in vivo."
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"Furthermore, we found the high expression of Ki67 in JOSD1-depleted xenografts, indicating that JOSD1 could accelerate tumor proliferation in HNSCC."
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"JOSD1 promotes proliferation and chemoresistance of head and neck squamous cell carcinoma under the epigenetic regulation of BRD4."
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JOSD1 inhibits cell population proliferation. 1 / 1
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"Furthermore, we found the high expression of Ki67 in JOSD1-depleted xenografts, indicating that JOSD1 could accelerate tumor proliferation in HNSCC."
34261480
JOSD1 affects HNSCC
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JOSD1 activates HNSCC.
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JOSD1 activates HNSCC. 3 / 3
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"Furthermore, we found the high expression of Ki67 in JOSD1-depleted xenografts, indicating that JOSD1 could accelerate tumor proliferation in HNSCC."
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"In summary, these data demonstrate that JOSD1 upregulation in HNSCC positively correlated with poor prognosis of patients, uncovering the clinical prognostic significance of JOSD1."
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"Compared with sensitive specimens, JOSD1 expression was dramatically increased in chemoresistant tissues (Fig. 1d), indicating a potential role of JOSD1 in HNSCC chemoresistance."
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JOSD1 inhibits HNSCC.
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JOSD1 inhibits HNSCC. 1 / 1
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"Furthermore, we found the high expression of Ki67 in JOSD1-depleted xenografts, indicating that JOSD1 could accelerate tumor proliferation in HNSCC."
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JOSD1 affects tumor growth
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JOSD1 activates tumor growth. 3 / 3
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"Additionally , JOSD1 suppression inhibited the tumor growth and improved chemosensitivity in vivo ."
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"JOSD1 knockdown impedes tumor growth and increases chemosensitivity of HNSCC cells in vivo ."
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"In our study , JOSD1 knockdown could inhibit the growth and colony formation of TSCCA and SCC25 cells , and suppress the tumor growth in vivo ."
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JOSD1 affects cell death
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JOSD1 activates cell death.
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JOSD1 activates cell death. 2 / 2
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"We found that overexpression of SOCS1 caused a decrease in CC3 expression and number of dead cells (Supplementary Fig 3A–C), while its downregulation conversely caused an increase in CC3 expression (Fig. 5A) and number of dead cells (Fig. 5B) under proteasomal inhibition in a manner similar to JOSD1.Next, we sought to determine whether SOCS1 sufficiency was a prerequisite for JOSD1’s ability to modulate cell death under proteotoxic conditions."
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"Consistently, adenovirus-mediated overexpression of JOSD1 in primary mouse hepatocytes caused a marked decline in cell death under proteotoxicity (Fig. 2C)."
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JOSD1 inhibits cell death.
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JOSD1 inhibits cell death. 1 / 1
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"A small molecule inhibitor of JOSD1 was shown to induce cell death of JAK2-V617F-positive primary acute myeloid leukemia (AML) cells [176]."
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JOSD1 affects JAK2-V617F
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JOSD1 deubiquitinates JAK2-V617F.
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JOSD1 deubiquitinates JAK2-V617F. 2 / 2
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"JOSD1 deubiquitinates and stabilises mutant JAK2 (JAK2-V617F), and therefore, inactivation of JOSD1 via small molecules led to the degradation of mutant JAK2 in MPNs providing a novel therapeutic approach for the disease [23]."
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"The preferential regulation of oncogenic mutated substrates by DUBs, as previously reported, where USP10 and JOSD1 selectively deubiquitinate FLT-mutants and JAK2-V617F, respectively , is also mirrored in the current study."
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JOSD1 binds JAK2-V617F.
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JOSD1 binds JAK2-V617F. 1 / 1
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"JOSD1 interacts with and stabilizes JAK2-V617F, and inactivation of the DUB leads to JAK2-V617F protein degradation by increasing its ubiquitination levels, thereby shortening its protein half-life."
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JOSD1 affects JAK2
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JOSD1 binds JAK2.
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JOSD1 binds JAK2. 1 / 2
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"In acute myeloid leukemia (AML) cells, JOSD1 interacts with JAK2-V617F and promotes its expression ( xref )."
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JOSD1 deubiquitinates JAK2.
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JOSD1 leads to the deubiquitination of mutated JAK2. 1 / 1
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"JOSD1 deubiquitinates and stabilises mutant JAK2 (JAK2-V617F), and therefore, inactivation of JOSD1 via small molecules led to the degradation of mutant JAK2 in MPNs providing a novel therapeutic approach for the disease [23]."
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TP53 affects JOSD1
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TP53 increases the amount of JOSD1.
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TP53 increases the amount of JOSD1. 1 / 1
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"Indeed, JOSD1 was up-regulated upon p53 inactivation, consistent with their anticorrelation in the unperturbed system, indicating a possible negative feedback loop in which p53 inhibits JOSD1 transcription, while the JOSD1 protein stabilizes p53."
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TP53 decreases the amount of JOSD1.
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TP53 decreases the amount of JOSD1. 1 / 1
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"Indeed, JOSD1 was up-regulated upon p53 inactivation, consistent with their anticorrelation in the unperturbed system, indicating a possible negative feedback loop in which p53 inhibits JOSD1 transcription, while the JOSD1 protein stabilizes p53."
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JQ1 affects JOSD1
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JQ1 inhibits JOSD1. 2 / 2
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"JQ1 treatment has been identified to downregulate both the JOSD1 protein and mRNA expression."
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"The results showed that the administration of JQ1 to HNSCC cells strikingly downregulated JOSD1 in mRNA and protein level (Fig. 2c, d)."
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"JOSD1 has been identified to negatively regulate IFN-I-induced signaling and the antiviral response by deubiquitinating the K48-linked polyubiquitination of SOCS1, which is an essential negative regulator of many cytokine signaling pathways (120)."
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"JOSD1 can inhibit the IFN-1-induced signalling pathway and antiviral response by physically interacting with SOCS1 and enhancing SOCS1 stability by cleaving K48-ubiquitin chains from SOCS1 [21]."
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JOSD1 affects cell motility
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JOSD1 activates cell motility. 1 / 2
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"Indeed, time-lapse imaging revealed that JosD1 enhances membrane dynamics and cell motility."
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JOSD1 affects cell growth
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JOSD1 activates cell growth. 2 / 2
| 1 1
eidos
"Under the regulation of BRD4 , JOSD1 is a key driver of cell growth and chemoresistance in vitro and in vivo ."
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reach
"Under the regulation of BRD4, JOSD1 is a key driver of cell growth and chemoresistance in vitro and in vivo."
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JOSD1 affects SNAI1
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JOSD1 deubiquitinates SNAI1. 2 / 2
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reach
"While data on JOSD1 in NBT are lacking, JOSD1 can deubiquitinate and stabilize Snail protein to promote EMT and tissue invasion of lung cancer cells [175]."
37892185
reach
"It was seen that JOSD1 could deubiquitinate and stabilise Snail protein which promoted epithelial to mesenchymal transition (EMT) in LUAD [24]."
39284830
JOSD1 affects JOSD1
| 2
JOSD1 increases the amount of JOSD1.
| 1
JOSD1 increases the amount of JOSD1. 1 / 1
| 1
reach
"Jing et al. found that under epigenetic regulation of BRD4, JOSD1 was overexpressed in HNSCC, and increased expression of JOSD1 was positively correlated with proliferation and chemoresistance of HNSCC cells, while highly expressed JOSD1 was also associated with poor prognosis of HNSCC patients [42]."
38012642
JOSD1 activates JOSD1.
| 1
JOSD1 activates JOSD1. 1 / 1
| 1
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"Compared with sensitive specimens, JOSD1 expression was dramatically increased in chemoresistant tissues (Fig. 1d), indicating a potential role of JOSD1 in HNSCC chemoresistance."
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JOSD1 affects GST
| 2
JOSD1 binds GST. 2 / 2
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sparser
"Subsequently, we examined the biological activity of GST-JOSD2/JOSD1 by introducing their co-reported substrate proteins YAP xref , xref , as shown in Supplementary Fig.  xref , both GST-JOSD2 and GST-JOSD1 efficiently removing the polyubiquitin chains of YAP, confirming that our purified recombinant protein are biologically active."
40240366
sparser
"More importantly, we performed in vitro deubiquitination assays using bacterial-expressed recombinant rhJOSD2 and found rhJOSD2 effectively removing the polyubiquitin chains of KRAS wild-type and mutants, while GST-JOSD1 failed to pose similar effects (Fig.  xref and Supplementary Fig.  xref )."
40240366
JAK2 affects JOSD1
1 | 1
JOSD1 binds JAK2. 1 / 2
1 | 1
sparser
"In acute myeloid leukemia (AML) cells, JOSD1 interacts with JAK2-V617F and promotes its expression ( xref )."
36159990
GST affects JOSD1
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JOSD1 binds GST. 2 / 2
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sparser
"Subsequently, we examined the biological activity of GST-JOSD2/JOSD1 by introducing their co-reported substrate proteins YAP xref , xref , as shown in Supplementary Fig.  xref , both GST-JOSD2 and GST-JOSD1 efficiently removing the polyubiquitin chains of YAP, confirming that our purified recombinant protein are biologically active."
40240366
sparser
"More importantly, we performed in vitro deubiquitination assays using bacterial-expressed recombinant rhJOSD2 and found rhJOSD2 effectively removing the polyubiquitin chains of KRAS wild-type and mutants, while GST-JOSD1 failed to pose similar effects (Fig.  xref and Supplementary Fig.  xref )."
40240366
JOSD1 binds suppressor of cytokine signalling. 1 / 1
| 1
sparser
"Mechanistically, JOSD1 physically interacts with the suppressor of cytokine signalling 1 (SOCS1), deubiquitinates it and enhances its stability under proteotoxic stress."
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JOSD1 binds suppressor of cytokine signalling 1. 1 / 1
| 1
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"Mechanistically, JOSD1 physically interacts with the suppressor of cytokine signalling 1 (SOCS1), deubiquitinates it and enhances its stability under proteotoxic stress."
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Positive regulation of NF-kappaB transcription factor activity activates JOSD1. 1 / 1
| 1
eidos
"We found that NF-kappaB activation through each of these agents elicited a significant increase in APOBEC3B gene expression with less robust activation of APOBEC3C and no effect on JOSD1 ."
33238122
Facilitating transcription activation affects JOSD1
| 1
Facilitating transcription activation activates JOSD1. 1 / 1
| 1
eidos
"Taken together , these evidences illustrate that BRD4 , as an epigenetic modulator , enhances the expression of JOSD1 by facilitating its transcription activation ."
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Epigenetic regulator BRD4 affects JOSD1
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Epigenetic regulator BRD4 activates JOSD1. 1 / 1
| 1
eidos
"The epigenetic regulator BRD4 contributed to the upregulation of JOSD1 in HNSCC ."
34261480
Cisplatin affects JOSD1
| 1
Cisplatin activates JOSD1. 1 / 1
| 1
reach
"The results of MTT assay showed that the IC50 value of CDDP was dramatically reduced in the HNSCC cells expressing JOSD1 shRNAs, indicating that JOSD1 knockdown could sensitize HNSCC cells to CDDP (Fig. 4c)."
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Administration JQ1 cells affects JOSD1
| 1
Administration JQ1 cells inhibits JOSD1. 1 / 1
| 1
eidos
"The results showed that the administration of JQ1 to HNSCC cells strikingly downregulated JOSD1 in mRNA and protein level ( Fig. 2c , d ) ."
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XMU-MP-1 affects JOSD1
| 1
XMU-MP-1 activates JOSD1. 1 / 1
| 1
reach
"Conversely, JOSD1 mRNA and protein levels in HCT116 and SW480 cells were increased by activation of YAP by XMU-MP-1 (Fig. 7M–P)."
39143074
Ubiquitin affects JOSD1
| 1
Ubiquitin-K48R activates JOSD1. 1 / 1
| 1
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"Consistently, the dominant negative mutant of ubiquitin (K48R) diminished the effect of JOSD1 on YAP poly-ubiquitination (Fig. 6F)."
39143074
USP21c affects JOSD1
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USP21c activates JOSD1. 1 / 1
| 1
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"In addition, USP21c has been shown to cause complete removal of ubiquitin chains from not only itself, but also JOSD1 (XREF_FIG b)."
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N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal inhibits JOSD1. 1 / 1
| 1
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"MG132 reduced the effect of JOSD1 removal on YAP protein levels (Fig. 5D, E)."
39143074
JOSD1 affects tumor cell
| 1
JOSD1 activates tumor cell. 1 / 1
| 1
eidos
"Furthermore , we found that JOSD1 knockdown suppressed tumor cell proliferation and metastasis , whereas overexpression of JOSD1 led to opposite phenotypes ."
35693075
JOSD1 affects tumor cell invasion
| 1
JOSD1 activates tumor cell invasion. 1 / 1
| 1
eidos
"Indeed , JOSD1 promoted tumor cell invasion as well as metastasis on the dependence of Snail ."
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JOSD1 binds suppressor of cytokine signalling. 1 / 1
| 1
sparser
"Mechanistically, JOSD1 physically interacts with the suppressor of cytokine signalling 1 (SOCS1), deubiquitinates it and enhances its stability under proteotoxic stress."
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JOSD1 binds suppressor of cytokine signalling 1. 1 / 1
| 1
reach
"Mechanistically, JOSD1 physically interacts with the suppressor of cytokine signalling 1 (SOCS1), deubiquitinates it and enhances its stability under proteotoxic stress."
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JOSD1 inhibits signal transduction. 1 / 1
| 1
reach
"JOSD1 can inhibit the IFN-1-induced signalling pathway and antiviral response by physically interacting with SOCS1 and enhancing SOCS1 stability by cleaving K48-ubiquitin chains from SOCS1 [21]."
39284830
JOSD1 affects signal cascade
| 1
JOSD1 inhibits signal cascade. 1 / 1
| 1
eidos
"JOSD1 inhibits the IFN signal cascade via deubiquitinating and stabilizing SOCS1 [ 92 ] ."
32650621
JOSD1 affects plasma membrane
| 1
JOSD1 inhibits plasma membrane. 1 / 1
| 1
reach
"In human cells, in addition to AMSH proteins , USP2 , USP6 , USP8/UBPY , USP9X , USP32 , and JosD1 have been shown to modulate the degradation of PM proteins."
36371501
JOSD1 affects pathway
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JOSD1 inhibits pathway. 1 / 1
| 1
sparser
"JOSD1 inhibits the mitochondrial apoptosis pathway and exerts anti-apoptosis effects and results in chemo-resistance in gynaecological cancer by stabilizing MCL1."
32982735
JOSD1 affects metastasis dependence Snail
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JOSD1 activates metastasis dependence Snail. 1 / 1
| 1
eidos
"Indeed , JOSD1 promoted tumor cell invasion as well as metastasis on the dependence of Snail ."
35693075
JOSD1 affects localization
| 1
JOSD1 activates localization. 1 / 1
| 1
reach
"The ubiquitination of JosD1 triggers its localization to the membrane from the cytoskeletal fraction [38]."
32512887
JOSD1 affects growth colony TSCCA SCC25 cells
| 1
JOSD1 activates growth colony TSCCA SCC25 cells. 1 / 1
| 1
eidos
"In our study , JOSD1 knockdown could inhibit the growth and colony formation of TSCCA and SCC25 cells , and suppress the tumor growth in vivo ."
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JOSD1 activates epithelial to mesenchymal transition. 1 / 1
| 1
reach
"We found that ATXN3 inhibited apoptosis, while JOSD1 and JOSD2 promoted apoptosis, JOSD2 inhibited DNA damage response, JOSD1 and JOSD2 promoted EMT, and JOSD2 suppressed RTK and androgen pathways.Moreover, we used the cBioPortal database to explore the co-expression (Table 3) and mutual exclusion (Table 4) between individual members of the MJDs family."
36159990
JOSD1 affects cisplatin-induced apoptosis HNSCC cells
| 1
JOSD1 inhibits cisplatin-induced apoptosis HNSCC cells. 1 / 1
| 1
eidos
"Moreover , JOSD1 depletion dramatically impaired cell proliferation and colony formation , and promoted cisplatin-induced apoptosis of HNSCC cells in vitro ."
34261480
JOSD1 affects chemosensitivity HNSCC cells
| 1
JOSD1 inhibits chemosensitivity HNSCC cells. 1 / 1
| 1
eidos
"JOSD1 knockdown impedes tumor growth and increases chemosensitivity of HNSCC cells in vivo ."
34261480
JOSD1 affects chemoresistance
| 1
JOSD1 activates chemoresistance. 1 / 1
| 1
eidos
"Under the regulation of BRD4 , JOSD1 is a key driver of cell growth and chemoresistance in vitro and in vivo ."
34261480
JOSD1 affects chemoresistance head neck squamous cell carcinoma epigenetic regulation enzymes
| 1
JOSD1 activates chemoresistance head neck squamous cell carcinoma epigenetic regulation enzymes. 1 / 1
| 1
eidos
"JOSD1 promotes proliferation and chemoresistance of head and neck squamous cell carcinoma under the epigenetic regulation of BRD4 Background Deubiquitinating enzymes ( DUBs ) play critical roles in various cancers by modulating functional proteins post-translationally ."
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JOSD1 affects cell apoptosis
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JOSD1 inhibits cell apoptosis. 1 / 1
| 1
eidos
"Moreover , with the treatment of CDDP , JOSD1 knockdown could increase expression of apoptosis-related proteins and promote cell apoptosis rate ."
34261480
JOSD1 affects capacity colony
| 1
JOSD1 activates capacity colony. 1 / 1
| 1
eidos
"Moreover , reduced level of JOSD1 impaired the capacity of colony formation in both TSCCA and SCC25 cells ( Fig. 5b ) ."
34261480
JOSD1 affects apoptosis-related proteins
| 1
JOSD1 inhibits apoptosis-related proteins. 1 / 1
| 1
eidos
"Moreover , with the treatment of CDDP , JOSD1 knockdown could increase expression of apoptosis-related proteins and promote cell apoptosis rate ."
34261480
JOSD1 affects apoptosis HNSCC cells treated CDDP flow cytometry
| 1
JOSD1 inhibits apoptosis HNSCC cells treated CDDP flow cytometry. 1 / 1
| 1
eidos
"Meanwhile , we found that depletion of JOSD1 led to a significant increase of apoptosis rate of HNSCC cells treated with CDDP by using flow cytometry ( Fig. 4d ) ."
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JOSD1 affects acquired chemoresistance
| 1
JOSD1 activates acquired chemoresistance. 1 / 1
| 1
eidos
"In gynaecological cancer , JOSD1 promotes acquired chemoresistance by stabilizing MCL1 [ 14 ] ."
34261480
JOSD1 affects Ubiquitin
| 1
JOSD1 inhibits Ubiquitin. 1 / 1
| 1
reach
"JOSD1, a deubiquitinase, and RNF7, a catalytic subunit of an E3 ligase complex, are pivotal for protein homeostasis by modulating ubiquitination dynamics, with JOSD1 reversing ubiquitin modifications and RNF7 facilitating neddylation and substrate recruitment for degradation ."
39574586
JOSD1 affects TEAD
| 1
JOSD1 activates TEAD. 1 / 1
| 1
reach
"In the luciferase assay, the wild type form of JOSD1 could increase the TEAD luciferase activity, while the JOSD1 could not (Fig. 3L)."
39143074
reach
"JOSD1 promotes proliferation and chemoresistance of head and neck squamous cell carcinoma under the epigenetic regulation of BRD4."
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JOSD1 inhibits Reactive Oxygen Species. 1 / 1
| 1
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"However, there was no change in the level of reactive oxygen species in JOSD1-depleted cells compared to control under proteasomal inhibition, indicating that JOSD1 operates in a ROS-independent pathway (Fig. 1D, bottom panel)."
39284830
JOSD1 affects RNF7
| 1
JOSD1 inhibits RNF7. 1 / 1
| 1
reach
"JOSD1, a deubiquitinase, and RNF7, a catalytic subunit of an E3 ligase complex, are pivotal for protein homeostasis by modulating ubiquitination dynamics, with JOSD1 reversing ubiquitin modifications and RNF7 facilitating neddylation and substrate recruitment for degradation ."
39574586
JOSD1 affects PARP1
| 1
JOSD1 inhibits PARP1. 1 / 1
| 1
reach
"A similar observation was seen in primary mouse hepatocytes where adenovirus-mediated knockdown of JOSD1 caused a robust increase in cell death as evidenced by increased expression of CC3 and cleaved PARP when subjected to proteasomal inhibition (Fig. 1E)."
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eidos
"Furthermore , we found that JOSD1 knockdown suppressed tumor cell proliferation and metastasis , whereas overexpression of JOSD1 led to opposite phenotypes ."
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reach
"A small molecule inhibitor of JOSD1 was shown to induce cell death of JAK2-V617F-positive primary acute myeloid leukemia (AML) cells [176]."
37892185
JOSD1 affects Hippo
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JOSD1 activates Hippo. 1 / 1
| 1
reach
"JOSD1 activates Hippo/YAP axis in colon cancer cells."
39143074
JOSD1 affects HYCC1
| 1
JOSD1 methylates HYCC1. 1 / 1
| 1
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"However, JOSD1 methylation levels in HCC tissues was higher than that in corresponding normal tissues without statistic difference, in spite of histological grades, TP53 mutations, lymph node metastatic, genders, and years (Figure 3C)."
36159990
JOSD1 affects HNSCC cell
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JOSD1 activates HNSCC cell. 1 / 1
| 1
eidos
"JOSD1 depletion inhibits HNSCC cell proliferation in vitro ."
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JOSD1 affects HMGCR
| 1
JOSD1 activates HMGCR. 1 / 1
| 1
reach
"To comprehensively identify specific DUBs, we transiently expressed a series of plasmids individually encoding 44 DUBs fused with the Flag protein in 293T cells, and found that USP8, USP10, BRCC36, and JOSD1 enhanced the HMGCR protein level (Figure S3A, Supporting Information)."
38178583
JOSD1 affects GOT
| 1
JOSD1 increases the amount of GOT. 1 / 1
| 1
reach
"Interestingly, the levels of plasma AST, a liver enzyme which serves as a marker for liver injury, was reciprocally increased upon JOSD1 knockdown and decreased by overexpression of JOSD1 (Fig. 6D)."
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JOSD1 affects Flag
| 1
JOSD1 binds Flag. 1 / 1
| 1
sparser
"Importantly, cell viability (Fig. xref ), plate colony formation (Fig. xref ) and anchorage-independent colony formation (Fig. xref ) were significantly restored after transfection of Flag-JOSD1 in TRAF4-null CAL27R cells."
36535926
JOSD1 affects Carcinogenesis
| 1
reach
"JOSD1 could facilitate colon cancer progression and in colon cancer, inhibition of JOSD1 via shRNA has been demonstrated to impede tumorigenesis."
39143074
JOSD1 affects CDDP treating HNSCC
| 1
JOSD1 inhibits CDDP treating HNSCC. 1 / 1
| 1
eidos
"Meanwhile , JOSD1 silencing combined with CDDP treatment extremely enhanced the positive staining of cleaved Caspase-3 compared with the other three groups ( Fig. 6c ) , which indicated that JOSD1 knockdown could improve the effect of CDDP for treating HNSCC in vivo ."
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JAK2-V617F affects JOSD1
| 1
JOSD1 binds JAK2-V617F. 1 / 1
| 1
reach
"JOSD1 interacts with and stabilizes JAK2-V617F, and inactivation of the DUB leads to JAK2-V617F protein degradation by increasing its ubiquitination levels, thereby shortening its protein half-life."
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Flag affects JOSD1
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JOSD1 binds Flag. 1 / 1
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sparser
"Importantly, cell viability (Fig. xref ), plate colony formation (Fig. xref ) and anchorage-independent colony formation (Fig. xref ) were significantly restored after transfection of Flag-JOSD1 in TRAF4-null CAL27R cells."
36535926
BRD4 shRNA resembling JQ1 affects JOSD1
| 1
BRD4 shRNA resembling JQ1 activates JOSD1. 1 / 1
| 1
eidos
"Besides , we found that knockdown of BRD4 by shRNA , resembling the effect of JQ1 , inhibited the expression of JOSD1 ( Fig. 2e , f ) , while BRD4 overexpression significantly upregulated JOSD1 in HNSCC cells ( Fig. 2g , h ) , suggesting that BRD4 affected JOSD1 expression at the transcriptional level ."
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BRD4 overexpression affects JOSD1
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BRD4 overexpression activates JOSD1. 1 / 1
| 1
eidos
"Besides , we found that knockdown of BRD4 by shRNA , resembling the effect of JQ1 , inhibited the expression of JOSD1 ( Fig. 2e , f ) , while BRD4 overexpression significantly upregulated JOSD1 in HNSCC cells ( Fig. 2g , h ) , suggesting that BRD4 affected JOSD1 expression at the transcriptional level ."
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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide activates JOSD1. 1 / 1
| 1
reach
"The results of MTT assay showed that the IC50 value of CDDP was dramatically reduced in the HNSCC cells expressing JOSD1 shRNAs, indicating that JOSD1 knockdown could sensitize HNSCC cells to CDDP (Fig. 4c)."
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