IndraLab

Statements

WDR48 affects USP1
2 43 | 1 479 555
WDR48 binds USP1.
1 43 | 431 542
WDR48 binds USP1. 10 / 979
1 43 | 415 509
reach
"15, NO. 19, 2636-2646 http://dx.doi.org/10.1080/15384101.2016.1209613REPORTThe USP1 and UAF1 complex interacts with RAD51AP1 to promote homologous recombination repairScott Cukrasa, Euiho Leea, Emily Palumboa, Pamela Benavideza, George-Lucian Moldovanb, and Younghoon KeeaaDepartment of Cell Biology, Microbiology, and Molecular Biology, College of Arts and Sciences, University of South Florida, Tampa, FL, USA; bDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, USAABSTRACTUSP1 deubiquitinating enzyme and its stoichiometric binding partner UAF1 play an essential role in promoting DNA homologous recombination (HR) repair in response to various types of DNA damaging agents."
27463890
sparser
"Further structural work will be required to elucidate how USP1‐UAF1 targets the ubiquitin on FANCI and how dsDNA may interfere with such targeting."
36385258
sparser
"Binding of USP1 by WDR48 was sensitive to deletions within the WD-repeats, indicating the importance of a presumed propeller domain ( Cohn et al., 2007 )."
26388029
sparser
"The end of the FA pathway is marked with the deubiquitination of FANCD2/ FANCI heterodimer by the USP1-UAF1 complex xref ."
38688950
sparser
"Indeed, the structure of USP1‐UAF1 complex bound to ID2 Ub ‐DNA revealed ID2 movements towards the open‐state conformation, affecting not only FANCI helices in the region where ubiquitin‐conjugation occurs, but also, and most profoundly, the FANCD2 N‐terminus involved in interaction with FANCI's ubiquitin (Rennie et al ,  xref )."
36385258
sparser
"The subsequent unhooking of ICL would be followed by TLS and deubiquitination of the ID complex with USP1-UAF1 complex."
38688950
sparser
"This model, however, seems difficult to reconcile with the finding that the carboxy-terminal region of USP1 is unable to interact with UAF1 [ xref ], and would require further experimental validation."
23937906
sparser
"To some extent, this may be due to USP1‐UAF1 preferentially targeting FANCD2, via USP1's N‐terminal extension (Arkinson et al ,  xref ) and through UAF1‐FANCI interactions acting as a USP1‐FANCD2 enzyme‐substrate recruitment module (Rennie et al ,  xref )."
36385258
sparser
"Indeed, we have shown here that disruption of this extended FANCD2‐ubiquitin interface (via FANCD2 mutagenesis), results in I Ub D2‐DNA being more sensitive to USP1‐UAF1 action (Fig  xref )."
36385258
reach
"We also show that a metabolic stabilization of Gln Usp1 Ct, a change that prolongs DUB activity of the autocleaved Usp1 in the Usp1 and Uaf1 complex, makes cells hypersensitive to UV irradiation."
23159736
USP1 binds WDR48 and RAD51AP1. 7 / 7
| 7
sparser
"We provide evidence that RAD51AP1 interacts with USP1 through UAF1, and that stability of RAD51AP1 is promoted by USP1 and UAF1."
27463890
sparser
"Thus, even though DNA binding by either UAF1 or RAD51AP1 is sufficient for targeting the USP1-UAF1-RAD51AP1 DUB complex to DNA for FANCD2 deubiquitination, the DNA binding attribute of both UAF1 and RAD51AP1 is required for optimal DNA damage repair."
31253762
sparser
"The USP1-UAF1 complex interacts with RAD51AP1 In order to search for the potential role of USP1 or UAF1 in HR repair, we undertook a proteomic approach to identify potential UAF1 or USP1 interacting proteins that regulate HR repair."
27463890
sparser
"Discussion Here we described a functional interaction between RAD51AP1 and the USP1-UAF1 deubiquitinating enzyme complex."
27463890
sparser
"USP1 and UAF1 interact with RAD51AP1."
27463890
sparser
"While Liang et al. found that the role of UAF1-RAD51AP1 is independent of USP1, our results indicate that USP1 and UAF1 interact with RAD51AP1 as a complex."
27463890
sparser
"UAF1-USP1 readily associates with RAD51AP1 to form a trimeric complex ( xref )."
27239033
Estrone binds WDR48 and USP1. 5 / 5
| 5
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
30890612
reach
"Additionally, a role for the E1, UAF1, and USP1 complex in the processing of late bidirectional theta RIs would explain why defective E1, UAF1, and USP1 complex formation has no effect on the progression of theta and unidirectional replication forks (XREF_FIG)."
30890612
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
30890612
reach
"The E1, UAF1, and USP1 complex is necessary for efficient replication of HPV11 episomes in U2OS cells."
30890612
reach
"The E1, UAF1, and USP1 complex contributes to the generation of HPV11 RIs produced by unidirectional replication."
30890612
USP1 binds WDR48 and TBK1. 5 / 5
| 5
sparser
"Mechanistically, UAF1 and USP1 bound to TBK1, removed K48-linked polyubiquitination of TBK1, and then stabilized its expression."
29138248
sparser
"UAF1 and USP1 interacted with TBK1 following LPS stimulation ( xref )."
29138248
sparser
"Mechanistically, USP1 and UAF1 bound to TBK1, removed its K48-linked polyubiquitination, and then reversed the degradation process of TBK1."
29138248
sparser
"Collectively, these results indicate that UAF1 and USP1 specifically bind to TBK1."
29138248
sparser
"UAF1 and USP1 interact with TBK1."
29138248
3A binds WDR48 and USP1. 3 / 3
| 3
sparser
"Since RAD51AP1, which stably associates with UAF1 xref , also possesses DNA binding activity xref , it was of considerable interest to test whether it would restore to USP1-UAF1 3A and USP1-UAF1 11A mutant complexes the ability to deubiquitinate FANCD2 on DNA."
31253762
sparser
"Our biochemical reconstitution studies (Fig.  xref and Supplementary Fig.  xref ) revealed that, via its DNA binding activity, RAD51AP1 targets the DNA binding defective USP1-UAF1 3A or USP1-UAF1 11A complex to deubiquitinate FANCD2 on DNA."
31253762
sparser
"To ask if UAF1 DNA-binding is relevant for ID2 deubiquitination, we examined the mutant USP1-UAF1 3A and USP1-UAF1 11A complexes (Supplementary Fig.  xref ) in FANCD2 deubiquitination."
31253762
HCC binds USP1 and WDR48. 3 / 3
| 3
sparser
"Moreover, we confirmed the interaction of USP1‐WDR48 in HCC cells using co‐immunoprecipitation."
32951278
sparser
"In addition, we confirmed the interaction between USP1 and WDR48 in HCC cell lines using co‐immunoprecipitation, and the results showed that USP1 interacted with WDR48 in MHCC97H and SK‐Hep‐1 cells (Figure  xref )."
32951278
sparser
"Validation of the interaction between USP1 and WDR48 in HCC cell lines."
32951278
WDR48 binds USP1, USP12, and USP46. 3 / 3
| 3
sparser
"UAF1 constitutively binds to USP1, USP12, and USP46, and this binding greatly enhances their deubiquitinase activity."
33247121
sparser
"For example, WDR48 interacts with USP1, USP12 and USP46 to activate their DUB activity ( xref ; xref )."
27880911
sparser
"For example, USP1, USP12 and USP46 can interact with WD40-repeat protein USP1-associated factor 1 (UAF1), and UAF1 stimulates the catalytic activity of human USP1 through the formation of a tight complex [ xref ]."
29386062
USP1 binds WDR48 and Ser313. 2 / 2
| 2
reach
"To assess whether the stability of the USP1 and UAF1 complex was affected by Ser313 to Ala mutation in USP1, the wild-type and mutant USP and UAF1 complexes were analyzed using native gel electrophoresis."
23116119
reach
"To confirm that the interaction between USP1 and UAF1 is mediated by USP1 Ser313 phosphorylation, six-His-tagged USP1 was coexpressed with untagged UAF1 in insect cells."
23116119
WDR48 binds USP1 and WD40 domain. 2 / 2
| 2
reach
"The SLD2 region of UAF1 binds FANCI and the WD40 domain of UAF1 binds and stimulates USP1."
28923280
reach
"USP1 forms a complex with UAF1 where its WD40 domain binds and stimulates the ubiquitin protease activity of USP1 to counteract the monoubiquitination of the FANCD2 and FANCI heterodimer [XREF_BIBR]."
30831436
ATAD5 binds WDR48 and USP1. 2 / 2
| 1 1
reach
"Our findings demonstrate that BAZ1B inhibits premature Ub-PCNA de-ubiquitination by the ATAD5-UAF1-USP1 complex."
39627214
sparser
"Furthermore, the ectopic expression of ATAD5 mutant protein (ATAD5 UAF1del) that does not interact with the USP1–UAF1 complex and results in defective PCNA deubiquitylation ( xref ; xref ) reduced chromatin-bound PCNA level similar to the ectopic expression of wild-type ATAD5 ( xref )."
23277426
WDR48 binds USP1 and USP46. 2 / 2
| 2
sparser
"Homologous amino acid motifs, mapping to the Fingers sub-domain, mediate binding of USP1 and USP46 to UAF1."
25744535
sparser
"Also, UAF1 can interact with USP1 and USP46 in addition to USP12 [ xref ]."
33498168
USP1 binds WDR48 and K48. 2 / 2
| 2
reach
"Hence, a deubiquitinase complex, USP1 and UAF1 complex, eliminates the K48-connected poly-ubiquitination of TBK1, blocks TBK1 protein degradation, and increases anti-viral responses [73] ."
35358582
reach
"Collectively, these data indicate that the USP1 and UAF1 complex selectively removes K48 linked ubiquitination of TBK1."
29138248
ELG1 binds WDR48, PCNA, and USP1. 1 / 1
| 1
sparser
"ELG1 (enhanced level of genomic instability 1) interacts with the USP1–UAF1 complex and supports USP1-mediated de-ubiquitination of PCNA [62] ."
28666590
Mutated USP1 binds WDR48. 1 / 1
| 1
reach
"Native gel electrophoresis of the wild-type and mutant USP1 and UAF1 complexes."
23116119
Helicase binds USP1 and WDR48. 1 / 1
| 1
sparser
"In this study, we showed that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 complex is necessary for the completion of bidirectional replication and the subsequent initiation of the unidirectional replication mechanism."
30890612
PALB2 binds helicase, USP1, and WDR48. 1 / 1
| 1
sparser
"In this report, we provide evidence that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 deubiquitinating enzyme complex, a member of the Fanconi anemia DNA damage response pathway, is required for the completion of the bidirectional theta replication of the HPV11 genome and the subsequent initiation of the unidirectional replication."
30890612
USP1 binds WDR48 and WDR20. 1 / 1
| 1
reach
"In addition, the USP1-Uaf-1-WDR20 complex directly deubiquitinates and stabilizes the Akt phosphatases PHLPP and PHLPPL, leading to a decrease in active pAkt levels, which then downregulates AR phosphorylation and enhances AR stability and transcriptional activity."
37752518
Ubiquitin binds WDR48 and USP1. 1 / 1
| 1
sparser
"Here we determine structures of human USP1-UAF1 with and without ubiquitin and bound to monoubiquitinated FANCI-FANCD2."
33795880
FANCD2 binds WDR48 and USP1. 1 / 1
| 1
sparser
"Alternatively, FANCI ubiquitination may directly disrupt USP1‐UAF1 binding to FANCD2."
32510829
USP1 binds WDR48 and Lys. 1 / 1
| 1
reach
"Very few USP structures where substrates have been trapped are known to date and include USP30, USP22, and USP1–UAF1 complexes with Lys -linked diubiquitin (32), mono-ubiquitinated H2B (33), and mono-ubiquitinated FANCI − FANCD2 (34), respectively."
37777157
ELG1 binds WDR48 and USP1. 1 / 1
| 1
sparser
"Clearly there will also be separate regulatory networks, for example ELG1 interacts with USP1-UAF1 and affects PCNA deubiquitylation, but not FANCD2 ubiquitylation status [ xref ]."
21605559
USP1 binds WDR48 and serine. 1 / 1
| 1
reach
"It has been shown that UAF1 activates USP1 through a potential active-site modulation, and the complex formation between USP1 and UAF1 is regulated by serine phosphorylation."
23797609
USP5 binds USP1, WDR48, and ATAD5. 1 / 1
| 1
sparser
"Intriguingly, human ATAD5 appears to respond differently from its yeast counterpart to PCNA modifications: whereas no preferential affinity for sumoylated PCNA was reported, Lee et al. observed an i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23688817
USP1 binds WDR48 and foci. 1 / 1
| 1
sparser
"The recruitment of USP1-UAF1 appears to be transient because ELG1 knockdown did not affect the levels of USP1 bound to chromatin, and in contrast to ELG1, neither USP1 nor UAF1 formed foci following DNA damage [ xref ]."
21640107
NLRP3 binds USP1 and WDR48. 1 / 1
| 1
sparser
"In vitro binding assays demonstrated that NLRP3 could directly interact with USP1 and UAF1 (Fig.  xref )."
33247121
WDR48 activates USP1.
1 | 1 45 13
WDR48 activates USP1. 10 / 65
1 | 1 44 13
reach
"Although the mechanisms by which these PTMs regulate DUB catalytic activity remain largely unknown, several recent detailed biochemical and structural studies have revealed some aspects of these mecha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23962487
reach
"UAF1 increases the basicity of the USP1 catalytic histidine, increasing its potency as a general base."
25702870
sparser
"Notable examples include USP7 activation by its HUBL domain and GMPS ( xref ), USP1 activation by UAF1 ( xref ), and Ubp8 activation in the SAGA complex ( xref )."
25702870
eidos
"In vitro , USP1 activity is greatly stimulated by USP1-associated factor 1 ( UAF1 ; also known as WDR48 ) , enhancing USP1 catalytic turnover ( kcat ) but not affinity ( Km ) for monoubiquitylated substrates74 ."
28959952
sparser
"Our finding that USP1 is activated by UAF1 prompted us to test whether the USP1/UAF1 complex is capable of deubiquitinating this physiologic substrate."
18082604
reach
"WDR48 stimulates the activity of the deubiquitylases USP1, USP12 and USP46."
40205037
sparser
"Using probes specific for the cysteine catalytic triad residues, we recently showed that UAF1 activates USP1 by modulating its active site conformation( xref )."
23116119
reach
"For example, USP1, USP12, and USP46 are activated by the WD40-repeat containing UAF1, and USP7 is activated by GMPS (Cohn et al., 2007, 2009; Faesen et al., 2011; van der Knaap et al., 2005)."
22195557
sparser
"This study confirmed that the modulator UAF1 activates USP1, USP12, and USP46, and GMPS activates USP7."
22195557
reach
"By using a fluorescent ubiquitin derivative Ub-AMC, Cohn et al demonstrated that UAF1 does not increase the affinity of USP1 for Ub-AMC, but robustly increases its catalytic rate."
19101576
WDR48 bound to USP1 activates USP1. 1 / 1
| 1
reach
"The formation of USP1 and UAF1 complex greatly stimulates the DUB activity of USP1."
23797609
WDR48 increases the amount of USP1.
| 1
WDR48 increases the amount of USP1. 1 / 2
| 1
reach
"Conditional knockout of Uaf1 in germ cells disrupts both the protein levels and localization of the deubiquitinating enzyme USP1, implicating altered ubiquitination in the observed spermiogenesis defects."
38591005
WDR48 inhibits USP1.
| 1
WDR48 inhibits USP1. 1 / 1
| 1
reach
"In vitro, USP1 activity is greatly stimulated by USP1-associated factor 1 (UAF1; also known as WDR48), enhancing USP1 catalytic turnover (k ) but not affinity (K ) for monoubiquitylated substrates ."
28959952
WDR48 deubiquitinates USP1.
| 1
WDR48 deubiquitinates USP1. 1 / 1
| 1
reach
"The E1 protein from anogenital HPV types interacts with the UAF1 associated deubiquitinating enzymes USP1, USP12, and USP46 to stimulate replication of the viral genome."
25833051
USP1 affects WDR48
1 43 | 1 441 542
USP1 binds WDR48.
1 43 | 431 542
WDR48 binds USP1. 10 / 979
1 43 | 415 509
reach
"15, NO. 19, 2636-2646 http://dx.doi.org/10.1080/15384101.2016.1209613REPORTThe USP1 and UAF1 complex interacts with RAD51AP1 to promote homologous recombination repairScott Cukrasa, Euiho Leea, Emily Palumboa, Pamela Benavideza, George-Lucian Moldovanb, and Younghoon KeeaaDepartment of Cell Biology, Microbiology, and Molecular Biology, College of Arts and Sciences, University of South Florida, Tampa, FL, USA; bDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, USAABSTRACTUSP1 deubiquitinating enzyme and its stoichiometric binding partner UAF1 play an essential role in promoting DNA homologous recombination (HR) repair in response to various types of DNA damaging agents."
27463890
sparser
"Further structural work will be required to elucidate how USP1‐UAF1 targets the ubiquitin on FANCI and how dsDNA may interfere with such targeting."
36385258
sparser
"Binding of USP1 by WDR48 was sensitive to deletions within the WD-repeats, indicating the importance of a presumed propeller domain ( Cohn et al., 2007 )."
26388029
sparser
"The end of the FA pathway is marked with the deubiquitination of FANCD2/ FANCI heterodimer by the USP1-UAF1 complex xref ."
38688950
sparser
"Indeed, the structure of USP1‐UAF1 complex bound to ID2 Ub ‐DNA revealed ID2 movements towards the open‐state conformation, affecting not only FANCI helices in the region where ubiquitin‐conjugation occurs, but also, and most profoundly, the FANCD2 N‐terminus involved in interaction with FANCI's ubiquitin (Rennie et al ,  xref )."
36385258
sparser
"The subsequent unhooking of ICL would be followed by TLS and deubiquitination of the ID complex with USP1-UAF1 complex."
38688950
sparser
"This model, however, seems difficult to reconcile with the finding that the carboxy-terminal region of USP1 is unable to interact with UAF1 [ xref ], and would require further experimental validation."
23937906
sparser
"To some extent, this may be due to USP1‐UAF1 preferentially targeting FANCD2, via USP1's N‐terminal extension (Arkinson et al ,  xref ) and through UAF1‐FANCI interactions acting as a USP1‐FANCD2 enzyme‐substrate recruitment module (Rennie et al ,  xref )."
36385258
sparser
"Indeed, we have shown here that disruption of this extended FANCD2‐ubiquitin interface (via FANCD2 mutagenesis), results in I Ub D2‐DNA being more sensitive to USP1‐UAF1 action (Fig  xref )."
36385258
reach
"We also show that a metabolic stabilization of Gln Usp1 Ct, a change that prolongs DUB activity of the autocleaved Usp1 in the Usp1 and Uaf1 complex, makes cells hypersensitive to UV irradiation."
23159736
USP1 binds WDR48 and RAD51AP1. 7 / 7
| 7
sparser
"We provide evidence that RAD51AP1 interacts with USP1 through UAF1, and that stability of RAD51AP1 is promoted by USP1 and UAF1."
27463890
sparser
"Thus, even though DNA binding by either UAF1 or RAD51AP1 is sufficient for targeting the USP1-UAF1-RAD51AP1 DUB complex to DNA for FANCD2 deubiquitination, the DNA binding attribute of both UAF1 and RAD51AP1 is required for optimal DNA damage repair."
31253762
sparser
"The USP1-UAF1 complex interacts with RAD51AP1 In order to search for the potential role of USP1 or UAF1 in HR repair, we undertook a proteomic approach to identify potential UAF1 or USP1 interacting proteins that regulate HR repair."
27463890
sparser
"Discussion Here we described a functional interaction between RAD51AP1 and the USP1-UAF1 deubiquitinating enzyme complex."
27463890
sparser
"USP1 and UAF1 interact with RAD51AP1."
27463890
sparser
"While Liang et al. found that the role of UAF1-RAD51AP1 is independent of USP1, our results indicate that USP1 and UAF1 interact with RAD51AP1 as a complex."
27463890
sparser
"UAF1-USP1 readily associates with RAD51AP1 to form a trimeric complex ( xref )."
27239033
Estrone binds WDR48 and USP1. 5 / 5
| 5
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
30890612
reach
"Additionally, a role for the E1, UAF1, and USP1 complex in the processing of late bidirectional theta RIs would explain why defective E1, UAF1, and USP1 complex formation has no effect on the progression of theta and unidirectional replication forks (XREF_FIG)."
30890612
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
30890612
reach
"The E1, UAF1, and USP1 complex is necessary for efficient replication of HPV11 episomes in U2OS cells."
30890612
reach
"The E1, UAF1, and USP1 complex contributes to the generation of HPV11 RIs produced by unidirectional replication."
30890612
USP1 binds WDR48 and TBK1. 5 / 5
| 5
sparser
"Mechanistically, UAF1 and USP1 bound to TBK1, removed K48-linked polyubiquitination of TBK1, and then stabilized its expression."
29138248
sparser
"UAF1 and USP1 interacted with TBK1 following LPS stimulation ( xref )."
29138248
sparser
"Mechanistically, USP1 and UAF1 bound to TBK1, removed its K48-linked polyubiquitination, and then reversed the degradation process of TBK1."
29138248
sparser
"Collectively, these results indicate that UAF1 and USP1 specifically bind to TBK1."
29138248
sparser
"UAF1 and USP1 interact with TBK1."
29138248
3A binds WDR48 and USP1. 3 / 3
| 3
sparser
"Since RAD51AP1, which stably associates with UAF1 xref , also possesses DNA binding activity xref , it was of considerable interest to test whether it would restore to USP1-UAF1 3A and USP1-UAF1 11A mutant complexes the ability to deubiquitinate FANCD2 on DNA."
31253762
sparser
"Our biochemical reconstitution studies (Fig.  xref and Supplementary Fig.  xref ) revealed that, via its DNA binding activity, RAD51AP1 targets the DNA binding defective USP1-UAF1 3A or USP1-UAF1 11A complex to deubiquitinate FANCD2 on DNA."
31253762
sparser
"To ask if UAF1 DNA-binding is relevant for ID2 deubiquitination, we examined the mutant USP1-UAF1 3A and USP1-UAF1 11A complexes (Supplementary Fig.  xref ) in FANCD2 deubiquitination."
31253762
HCC binds USP1 and WDR48. 3 / 3
| 3
sparser
"Moreover, we confirmed the interaction of USP1‐WDR48 in HCC cells using co‐immunoprecipitation."
32951278
sparser
"In addition, we confirmed the interaction between USP1 and WDR48 in HCC cell lines using co‐immunoprecipitation, and the results showed that USP1 interacted with WDR48 in MHCC97H and SK‐Hep‐1 cells (Figure  xref )."
32951278
sparser
"Validation of the interaction between USP1 and WDR48 in HCC cell lines."
32951278
WDR48 binds USP1, USP12, and USP46. 3 / 3
| 3
sparser
"UAF1 constitutively binds to USP1, USP12, and USP46, and this binding greatly enhances their deubiquitinase activity."
33247121
sparser
"For example, WDR48 interacts with USP1, USP12 and USP46 to activate their DUB activity ( xref ; xref )."
27880911
sparser
"For example, USP1, USP12 and USP46 can interact with WD40-repeat protein USP1-associated factor 1 (UAF1), and UAF1 stimulates the catalytic activity of human USP1 through the formation of a tight complex [ xref ]."
29386062
USP1 binds WDR48 and Ser313. 2 / 2
| 2
reach
"To assess whether the stability of the USP1 and UAF1 complex was affected by Ser313 to Ala mutation in USP1, the wild-type and mutant USP and UAF1 complexes were analyzed using native gel electrophoresis."
23116119
reach
"To confirm that the interaction between USP1 and UAF1 is mediated by USP1 Ser313 phosphorylation, six-His-tagged USP1 was coexpressed with untagged UAF1 in insect cells."
23116119
WDR48 binds USP1 and WD40 domain. 2 / 2
| 2
reach
"The SLD2 region of UAF1 binds FANCI and the WD40 domain of UAF1 binds and stimulates USP1."
28923280
reach
"USP1 forms a complex with UAF1 where its WD40 domain binds and stimulates the ubiquitin protease activity of USP1 to counteract the monoubiquitination of the FANCD2 and FANCI heterodimer [XREF_BIBR]."
30831436
ATAD5 binds WDR48 and USP1. 2 / 2
| 1 1
reach
"Our findings demonstrate that BAZ1B inhibits premature Ub-PCNA de-ubiquitination by the ATAD5-UAF1-USP1 complex."
39627214
sparser
"Furthermore, the ectopic expression of ATAD5 mutant protein (ATAD5 UAF1del) that does not interact with the USP1–UAF1 complex and results in defective PCNA deubiquitylation ( xref ; xref ) reduced chromatin-bound PCNA level similar to the ectopic expression of wild-type ATAD5 ( xref )."
23277426
WDR48 binds USP1 and USP46. 2 / 2
| 2
sparser
"Homologous amino acid motifs, mapping to the Fingers sub-domain, mediate binding of USP1 and USP46 to UAF1."
25744535
sparser
"Also, UAF1 can interact with USP1 and USP46 in addition to USP12 [ xref ]."
33498168
USP1 binds WDR48 and K48. 2 / 2
| 2
reach
"Hence, a deubiquitinase complex, USP1 and UAF1 complex, eliminates the K48-connected poly-ubiquitination of TBK1, blocks TBK1 protein degradation, and increases anti-viral responses [73] ."
35358582
reach
"Collectively, these data indicate that the USP1 and UAF1 complex selectively removes K48 linked ubiquitination of TBK1."
29138248
ELG1 binds WDR48, PCNA, and USP1. 1 / 1
| 1
sparser
"ELG1 (enhanced level of genomic instability 1) interacts with the USP1–UAF1 complex and supports USP1-mediated de-ubiquitination of PCNA [62] ."
28666590
Mutated USP1 binds WDR48. 1 / 1
| 1
reach
"Native gel electrophoresis of the wild-type and mutant USP1 and UAF1 complexes."
23116119
Helicase binds USP1 and WDR48. 1 / 1
| 1
sparser
"In this study, we showed that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 complex is necessary for the completion of bidirectional replication and the subsequent initiation of the unidirectional replication mechanism."
30890612
PALB2 binds helicase, USP1, and WDR48. 1 / 1
| 1
sparser
"In this report, we provide evidence that the previously described interaction between the viral E1 helicase and the cellular UAF1-USP1 deubiquitinating enzyme complex, a member of the Fanconi anemia DNA damage response pathway, is required for the completion of the bidirectional theta replication of the HPV11 genome and the subsequent initiation of the unidirectional replication."
30890612
USP1 binds WDR48 and WDR20. 1 / 1
| 1
reach
"In addition, the USP1-Uaf-1-WDR20 complex directly deubiquitinates and stabilizes the Akt phosphatases PHLPP and PHLPPL, leading to a decrease in active pAkt levels, which then downregulates AR phosphorylation and enhances AR stability and transcriptional activity."
37752518
Ubiquitin binds WDR48 and USP1. 1 / 1
| 1
sparser
"Here we determine structures of human USP1-UAF1 with and without ubiquitin and bound to monoubiquitinated FANCI-FANCD2."
33795880
FANCD2 binds WDR48 and USP1. 1 / 1
| 1
sparser
"Alternatively, FANCI ubiquitination may directly disrupt USP1‐UAF1 binding to FANCD2."
32510829
USP1 binds WDR48 and Lys. 1 / 1
| 1
reach
"Very few USP structures where substrates have been trapped are known to date and include USP30, USP22, and USP1–UAF1 complexes with Lys -linked diubiquitin (32), mono-ubiquitinated H2B (33), and mono-ubiquitinated FANCI − FANCD2 (34), respectively."
37777157
ELG1 binds WDR48 and USP1. 1 / 1
| 1
sparser
"Clearly there will also be separate regulatory networks, for example ELG1 interacts with USP1-UAF1 and affects PCNA deubiquitylation, but not FANCD2 ubiquitylation status [ xref ]."
21605559
USP1 binds WDR48 and serine. 1 / 1
| 1
reach
"It has been shown that UAF1 activates USP1 through a potential active-site modulation, and the complex formation between USP1 and UAF1 is regulated by serine phosphorylation."
23797609
USP5 binds USP1, WDR48, and ATAD5. 1 / 1
| 1
sparser
"Intriguingly, human ATAD5 appears to respond differently from its yeast counterpart to PCNA modifications: whereas no preferential affinity for sumoylated PCNA was reported, Lee et al. observed an i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23688817
USP1 binds WDR48 and foci. 1 / 1
| 1
sparser
"The recruitment of USP1-UAF1 appears to be transient because ELG1 knockdown did not affect the levels of USP1 bound to chromatin, and in contrast to ELG1, neither USP1 nor UAF1 formed foci following DNA damage [ xref ]."
21640107
NLRP3 binds USP1 and WDR48. 1 / 1
| 1
sparser
"In vitro binding assays demonstrated that NLRP3 could directly interact with USP1 and UAF1 (Fig.  xref )."
33247121
USP1 activates WDR48.
| 1 8
USP1 activates WDR48. 9 / 10
| 1 8
reach
"The possibility that the NLS inactivating mutations might simultaneously interfere with UAF1 binding can not be formally ruled out, but these results suggest that the inability of USP1 (235-408) to promote UAF1 relocation can not be ascribed to competition with the nuclear import machinery."
25744535
reach
"Further studies of the USP1/UAF1 inhibitors suggested that pimozide and GW7647 inhibit USP1/UAF1 by a non‐competitive mechanism and bind at a site other than the active site of USP1/UAF1 [113]."
24239534
reach
"These results indicated that UAF1 suppressed JEV replication.As the cofactor of USP1, USP12, and USP46, UAF1 could enhance their deubiquitinase activity by forming stable UAF1/USP protein complexes (6, 38)."
36988464
reach
"Moreover, we verified that (i) UAF1-USP1 is no more effective than UAF1 in the D-loop reaction (XREF_SUPPLEMENTARY), and (ii) USP1 fails to restore functional synergy of the UAF1 EEA mutant with RAD51AP1 (XREF_SUPPLEMENTARY)."
27239033
reach
"Moreover, WDR48 was significantly reduced by USP1 knockdown in MHCC97H and SK‐Hep‐1 cells (Figure 10B,C,E,F)."
32951278
reach
"This assay is based on the nuclear accumulation of UAF1 induced by USP1 co-expression and, by using fluorescently tagged versions of these proteins, the results can be observed in live cells, before processing for microscopy analysis."
25744535
eidos
"Moreover , WDR48 was significantly reduced by USP1 knockdown in MHCC97H and SK-Hep-1 cells ( Figure 10B , C , E , F ) ."
32951278
reach
"USP1, the major DUB of FANCD2 and FANCI, inactivates these two proteins mediated by the USP1 activating factor UAF1 once DNA damage repair is finished [XREF_BIBR]."
28031783
reach
"It raises the possibility that a targeted knockout of Uaf1 in granulosa cells might lead to infertility in female mice, which could provide further insights into UAF1’s specific functions in female reproductive biology.UAF1 acts as a cofactor for USP1, USP12, and USP46, enhancing their deubiquitinase activity by forming stable UAF1/USP protein complexes.14 18 Defective spermiogenesis in Uaf1 sKO mice necessitated the identification of a DUB critical for this process."
38591005
USP1 inhibits WDR48.
| 1
USP1-E444K inhibits WDR48. 1 / 1
| 1
reach
"Likewise, mutation of USP1 E444K, analogous to the impactful USP46 E186K mutation, abrogated WDR48 binding and stimulation of USP1 deubiquitinase activity."
26388029
USP1 deubiquitinates WDR48.
| 1
USP1 deubiquitinates WDR48. 1 / 1
| 1
reach
"To test whether this extended interface is involved in protecting FANCI from deubiquitination, we mutated H209, V243 and P244 of FANCD2 to alanine residues, and assessed I ‐DNA deubiquitination by USP1‐UAF1 in the presence of wild‐type (D2 ) or H209A/V243A/P244A mutated (D2 ) FANCD2."
36385258