IndraLab

Statements

MPND affects diphosphate(4-)

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MPND activates diphosphate(4-). 3 / 3

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"Interestingly, the indolactam specific TleC and MpnD both accepted different chain lengths (from C 5 to C 25) of prenyl pyrophosphate in vitro."

26952246

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"Interestingly, both TleC and MpnD also accepted farnesyl pyrophosphate (FPP) (C 15), geranylgeranyl pyrophosphate (GGPP) (C 20) and geranylfarnesyl pyrophosphate (GFPP) (C 25) as prenyl donors, to produce 5, 6 and 7, respectively (XREF_FIG and XREF_SUPPLEMENTARY)."

26952246

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"In contrast to typical indole PTs that exhibit broad substrate specificities towards the prenyl acceptors but with strict specificities towards the prenyl donors XREF_BIBR XREF_BIBR XREF_BIBR XREF_BIBR, it is remarkable that the indolactam specific TleC and MpnD exhibited unusually relaxed substrate specificities towards the prenyl donors and accepted different chain lengths (from C 5 to C 25) of prenyl pyrophosphate in vitro."

26952246

Valproic acid affects MPND

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Valproic acid methylates MPND.

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Valproic acid methylates MPND. 1 / 1

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ctd

No evidence text available

29154799

Valproic acid decreases the amount of MPND.

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Valproic acid decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

15901671

TleC affects MPND

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MPND binds TleC. 2 / 2

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"The structural analysis of MpnD and TleC complexed with substrates suggested that the three amino acid residues Trp97, Phe170, and Ala173 in TleC and Tyr80, Trp157, and Met159 in MpnD regulate the selectivity of the length of prenyl donor and regiospecificity of prenylation position."

32180104

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"Furthermore, the crystal structures of TleC and MpnD complexed with the substrates suggested that the distance between the C-3 position of DMSPP and the C-7 position of 1 (3.3 A in TleC and 3.1 A in MpnD) is closer than that between the C-1 position of the prenyl substrates and the N-1 position of 1 (6.0 A in TleC and MpnD) (XREF_SUPPLEMENTARY)."

26952246

MPND is modified

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MPND is phosphorylated on S181. 1 / 1

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hprd

No evidence text available

17081983

MPND is phosphorylated on S178. 1 / 1

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hprd

No evidence text available

17081983

MPND affects prenyl diphosphate

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MPND activates prenyl diphosphate. 2 / 2

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"Thus, TleC and MpnD clearly prefer to accept GPP and DMAPP as the prenyl donors, respectively."

26952246

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"First, the indolactam specific TleC and MpnD both accepted DMAPP (C 5) and GPP (C 10) as substrates to produce pendolmycin (3), and lyngbyatoxin A (2) and 5-geranylindolactam V (4) (2:4 = 26:1 for TleC; 2:4 = 1:3 for MpnD), respectively (XREF_FIG)."

26952246

MPND affects TleC

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MPND binds TleC. 2 / 2

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32180104

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26952246

MPND affects DEG15

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MPND activates DEG15. 2 / 2

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26952246

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"Thus, TleC and MpnD clearly prefer to accept GPP and DMAPP as the prenyl donors, respectively."

26952246

Trichostatin A affects MPND

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Trichostatin A decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

15901671

Thioacetamide affects MPND

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Thioacetamide decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

23411599

Tetrachloroethene affects MPND

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Tetrachloroethene decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

28973375

Sulforaphane affects MPND

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Sulforaphane decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

31838189

Pregnenolone 16alpha-carbonitrile affects MPND

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Pregnenolone 16alpha-carbonitrile increases the amount of MPND. 1 / 1

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ctd

No evidence text available

28903501

Methylmercury chloride affects MPND

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Methylmercury chloride decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

28001369

Methapyrilene affects MPND

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Methapyrilene demethylates MPND. 1 / 1

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ctd

No evidence text available

30157460

Jinfukang affects MPND

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Jinfukang increases the amount of MPND. 1 / 1

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ctd

No evidence text available

27392435

Hsa-miR-335-5p affects MPND

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Hsa-miR-335-5p decreases the amount of MPND. 1 / 1

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biopax:mirtarbase

No evidence text available

18185580

Hsa-let-7b-5p affects MPND

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Hsa-let-7b-5p decreases the amount of MPND. 1 / 1

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biopax:mirtarbase

No evidence text available

23622248

Hexabromocyclododecane affects MPND

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Hexabromocyclododecane decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

29177809

Genistein affects MPND

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Genistein increases the amount of MPND. 1 / 1

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ctd

No evidence text available

18847459

Cyclosporin A affects MPND

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Cyclosporin A decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

20106945

Copper(II) sulfate affects MPND

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Copper(II) sulfate decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

19549813

Copper atom affects MPND

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Copper atom increases the amount of MPND. 1 / 1

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ctd

No evidence text available

30556269

Cobalt dichloride affects MPND

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Cobalt dichloride decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

24386269

Clofibrate affects MPND

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Clofibrate decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

23811191

Bisphenol A affects MPND

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Bisphenol A methylates MPND. 1 / 1

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ctd

No evidence text available

28505145

Benzo[e]pyrene affects MPND

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Benzo[e]pyrene demethylates MPND. 1 / 1

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ctd

No evidence text available

30157460

Benzo[a]pyrene affects MPND

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Benzo[a]pyrene decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

20106945

Atrazine affects MPND

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Atrazine decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

22378314

Aflatoxin B1 affects MPND

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Aflatoxin B1 decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

27153756

Abrine affects MPND

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Abrine decreases the amount of MPND. 1 / 1

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ctd

No evidence text available

31054353

USP36 affects MPND

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USP36 binds MPND. 1 / 1

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biogrid

No evidence text available

19615732

USP30 affects MPND

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USP30 binds MPND. 1 / 1

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biogrid

No evidence text available

19615732

MPND affects tumor penetration

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MPND activates tumor penetration. 1 / 1

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eidos

"Furthermore , using multicellular tumor spheroids to mimic in vivo experiment , MPND can enhance the tumor penetration and therapeutic effect for killing the deep tumor tissue at the central location ."

35007003

MPND affects pPAJV643

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MPND activates pPAJV643. 1 / 1

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eidos

"The mpnD gene encodes a prenyltransferase that , unlike TleC or LtxC , transfers a dimethylallyl group to the C-7 position of ILV to complete the biosynthesis of pendolmycin.7 Like the verification of tleC above , mpnD was cloned into the position of ltxC to create pPAJV643 ( ltxAB-mpnD ) ."

31846576

MPND affects migration breast cancer cells

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MPND inhibits migration breast cancer cells. 1 / 1

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eidos

"Results showed that MPND causes enhanced bioavailability , better cellular uptake , and inhibition of the migration of breast cancer cells as compared to the drug alone ."

35423422

MPND affects cellular uptake

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MPND activates cellular uptake. 1 / 1

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eidos

"Results showed that MPND causes enhanced bioavailability , better cellular uptake , and inhibition of the migration of breast cancer cells as compared to the drug alone ."

35423422

MPND affects active proteins

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MPND activates active proteins. 1 / 1

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eidos

"These results show that the codon-optimized tleC , tleD , and mpnD all produce active proteins with the expected activity when heterologously expressed in Anabaena 7120.9 , 15 This demonstrates that the native levels of transcriptional and translational regulation exerted when these genes occupy the same positions as ltxC ( tleC , mpnD ) and ltxD ( tleD ) in the ltx gene cluster are sufficient to produce proteins that efficiently catalyzed the desired reactions ; each protein completely converted its substrate to the expected product ( s ) because 1 was not observed in extracts of ltxABC-tleD or ltxAB-mpnD ."

31846576

MPND affects USP36

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USP36 binds MPND. 1 / 1

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biogrid

No evidence text available

19615732

MPND affects USP30

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USP30 binds MPND. 1 / 1

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biogrid

No evidence text available

19615732

MPND affects Therapeutics

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MPND activates Therapeutics. 1 / 1

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eidos

35007003

MPND affects Neoplasms

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MPND activates Neoplasms. 1 / 1

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"Furthermore, using multicellular tumor spheroids to mimic in vivo experiment, MPND can enhance the tumor penetration and therapeutic effect for killing the deep tumor tissue at the central location."

35007003

MPND affects Histone

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Histone binds MPND. 1 / 1

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"Moreover, the DNA and the two acidic regions of MPND synergistically enhance the interaction between MPND and histones."

36834777

MPND affects E7

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MPND binds E7. 1 / 1

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virhostnet

No evidence text available

28786561

MPND affects DOX

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MPND decreases the amount of DOX. 1 / 1

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"When the MPND is internalized into the cancer cell, the rupture of -S-S- bridges and the degradation of MPND occur in the tumor microenvironment with a high level of GSH, inducing the on-demand release of DOX."

35007003

MPND affects C5

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MPND activates C5. 1 / 1

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"This would be the reason why MpnD produces not only the C-7 ' reverse ' prenylation product 2 but also the C-5 ' normal ' prenylation product 4."

26952246

MPND affects Biological Availability

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MPND activates Biological Availability. 1 / 1

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eidos

"Results showed that MPND causes enhanced bioavailability , better cellular uptake , and inhibition of the migration of breast cancer cells as compared to the drug alone ."

35423422