IndraLab

Statements

UBR5 affects OTUD5
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UBR5 binds OTUD5.
5 | 15 24
OTUD5 binds UBR5. 10 / 43
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"UBR5-OTUD5 complex mitigates DNA replication fork stress."
37713620
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"UBR5-OTUD5 complex mitigates transcription-replication conflicts."
37713620
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"We hypothesized that the OTUD5-UBR5 complex may mitigate TRCs, based on our previous finding that the OTUD5-UBR5 complex regulates DNA double strand break (DSB)-induced transcription arrest, and that OTUD5-UBR5 proteins are present at replication forks (Figure 1G)."
37713620
sparser
"DUBA is associated with the UBR5 (a ubiquitin ligase), suppressing abundance of DUBA in naive T cells."
40821838
sparser
"UBR5 co-precipitates with OTUD5 (Figure xref ), and the purified recombinant OTUD5 and UBR5 proteins interact in vitro (Figure xref ; xref for western blot), suggesting that they interact directly."
30508113
sparser
"PLA detected the interaction between UBR5 and OTUD5 at damaged chromatin in situ , and that the interaction spots partially coincide with the DNA DSB marker 53BP1 (Figure xref ), suggesting that at least a fraction of the interaction indeed occurs at damage (DSB) sites."
30508113
sparser
"Previous studies report that OTUD5 interacts with UBR5, a HECT-type K48-linkage specific E3, in the regulation of DNA damage response and NF-κB signaling xref , xref ."
40128189
sparser
"Mass spectrometry coupled to short interfering RNA (siRNA) screening of ubiquitinated proteins in Duba −/− Th17 cells revealed that DUBA directly bound and deubiquitinated UBR5 (Ubiquitin protein ligase E3 component n-recognin 5)."
34635393
sparser
"Transcriptional repression around DSB is also influenced by histone modifiers NuRD ( xref ), KDMA5( xref ), FACT histone chaperone regulators UBR5–OTUD5 ( xref , xref ), RNAPII elongation regulators NELFE ( xref ), ENL ( xref ), and DYRK1B ( xref )."
40440073
reach
"We could confirm the interactions between OTUD5 and ARID1A/B, HDAC2, HCF1, and UBR5 by immunoblotting at the endogenous level in hES H1 cells (XREF_FIG)."
| PMC
OTUD5 binds HDAC2, HCFC1, and UBR5. 1 / 1
| 1
sparser
"We could confirm the interactions between OTUD5 and ARID1A/B, HDAC2, HCF1, and UBR5 by immunoblotting at the endogenous level in hES H1 cells ( xref )."
| PMC
UBR5 ubiquitinates OTUD5.
| 3 5
UBR5 ubiquitinates OTUD5. 8 / 8
| 3 5
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"UBR5-dependent ubiquitylation of OTUD5 was almost completely diminished with TRIP12 knockdown (Fig. 2g, lanes 2 and 4), whereas TRIP12-dependent ubiquitylation was partially decreased by UBR5 knockdown (Fig. 2h, lanes 2 and 4, and supplementary Fig. 1d)."
40128189
sparser
"UBR5-dependent ubiquitylation of OTUD5 was almost completely diminished with TRIP12 knockdown (Fig.  xref , lanes 2 and 4), whereas TRIP12-dependent ubiquitylation was partially decreased by UBR5 knockdown (Fig.  xref , lanes 2 and 4, and supplementary Fig.  xref )."
40128189
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"Notably, UBR5 ubiquitylation of wild-type OTUD5 was less effective than that of C224R OTUD5 (Fig. 5b (i))."
40128189
sparser
"The data on a catalytically-inactive OTUD5 (Fig.  xref ) suggest that the ubiquitylation of OTUD5 by TRIP12 and UBR5 appears as if they act sequentially because OTUD5 DUB activity negates UBR5 activity, which is different from typical chain initiation (priming)-elongation model xref , xref ."
40128189
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"Since UBR5 can stably associate with OTUD5 xref , UBR5 may transiently ubiquitylate OTUD5 with K48-linked chains."
40128189
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"The data on a catalytically-inactive OTUD5 (Fig. 5) suggest that the ubiquitylation of OTUD5 by TRIP12 and UBR5 appears as if they act sequentially because OTUD5 DUB activity negates UBR5 activity, which is different from typical chain initiation (priming)-elongation model ."
40128189
sparser
"Next, we investigated whether OTUD5 DUB activity counteracted OTUD5 ubiquitylation by TRIP12 or UBR5."
40128189
sparser
"Notably, UBR5 ubiquitylation of wild-type OTUD5 was less effective than that of C224R OTUD5 (Fig.  xref (i))."
40128189
UBR5 activates OTUD5.
| 2
UBR5 activates OTUD5. 2 / 2
| 2
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"UBR5 destabilizes DUBA through ubiquitylation, whereas DUBA stabilizes UBR5 in activated T cells by attenuating degradative auto-ubiquitylation, triggering UBR5 mediated ubiquitylation of the transcription factor RORyt in response to TGF-beta [XREF_BIBR]."
27834739
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"Considering other potential post-transcriptional mechanisms, we investigated the ubiquitin ligase UBR5, which has been reported to mediate OTUD5 abundance post-transcriptionally ."
38110369
UBR5 inhibits OTUD5.
| 1
UBR5 bound to OTUD5 inhibits OTUD5. 1 / 1
| 1
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"DUBA interacted with the ubiquitin ligase UBR5, which suppressed DUBA abundance in naive T cells."
25470037
OTUD5 affects UBR5
5 1 | 17 24
OTUD5 binds UBR5.
5 | 15 24
OTUD5 binds UBR5. 10 / 43
5 | 15 23
reach
"UBR5-OTUD5 complex mitigates DNA replication fork stress."
37713620
reach
"UBR5-OTUD5 complex mitigates transcription-replication conflicts."
37713620
reach
"We hypothesized that the OTUD5-UBR5 complex may mitigate TRCs, based on our previous finding that the OTUD5-UBR5 complex regulates DNA double strand break (DSB)-induced transcription arrest, and that OTUD5-UBR5 proteins are present at replication forks (Figure 1G)."
37713620
sparser
"DUBA is associated with the UBR5 (a ubiquitin ligase), suppressing abundance of DUBA in naive T cells."
40821838
sparser
"UBR5 co-precipitates with OTUD5 (Figure xref ), and the purified recombinant OTUD5 and UBR5 proteins interact in vitro (Figure xref ; xref for western blot), suggesting that they interact directly."
30508113
sparser
"PLA detected the interaction between UBR5 and OTUD5 at damaged chromatin in situ , and that the interaction spots partially coincide with the DNA DSB marker 53BP1 (Figure xref ), suggesting that at least a fraction of the interaction indeed occurs at damage (DSB) sites."
30508113
sparser
"Previous studies report that OTUD5 interacts with UBR5, a HECT-type K48-linkage specific E3, in the regulation of DNA damage response and NF-κB signaling xref , xref ."
40128189
sparser
"Mass spectrometry coupled to short interfering RNA (siRNA) screening of ubiquitinated proteins in Duba −/− Th17 cells revealed that DUBA directly bound and deubiquitinated UBR5 (Ubiquitin protein ligase E3 component n-recognin 5)."
34635393
sparser
"Transcriptional repression around DSB is also influenced by histone modifiers NuRD ( xref ), KDMA5( xref ), FACT histone chaperone regulators UBR5–OTUD5 ( xref , xref ), RNAPII elongation regulators NELFE ( xref ), ENL ( xref ), and DYRK1B ( xref )."
40440073
reach
"We could confirm the interactions between OTUD5 and ARID1A/B, HDAC2, HCF1, and UBR5 by immunoblotting at the endogenous level in hES H1 cells (XREF_FIG)."
| PMC
OTUD5 binds HDAC2, HCFC1, and UBR5. 1 / 1
| 1
sparser
"We could confirm the interactions between OTUD5 and ARID1A/B, HDAC2, HCF1, and UBR5 by immunoblotting at the endogenous level in hES H1 cells ( xref )."
| PMC
OTUD5 deubiquitinates UBR5.
1 | 1
OTUD5 deubiquitinates UBR5. 2 / 2
1 | 1
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"When DUBA deubiquitylates UBR5 and rescues it from degradation, UBR5 is free to polyubiquitylate RORgammaT, driving its degradation and consequently limiting Th17 differentiation."
27183634
ubibrowser
"<span class="match term0">DUBA</span> interacted with the ubiquitin ligase <span class="match term1">UBR5</span>, which suppressed <span class="match term0">DUBA</span> abundance in naive T cells. <span class="match term0">DUBA</span> accumulated in activated T cells and stabilized <span class="match term1">UBR5</span>, which then ubiquitylated RORgammat in response to TGF-beta signalling. "
25470037
OTUD5 inhibits UBR5.
| 1
OTUD5 inhibits UBR5. 1 / 1
| 1
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"These properties of the three enzymes constitute a complex “rock–paper–scissors”-like interplay.OTUD5 protects UBR5 from self-ubiquitylation , suggesting that OTUD5 antagonizes UBR5-mediated K48 ubiquitylation."
40128189