"In agreement, pretreatment of MCF-7 cells with 17-beta-estradiol resulted in a reduced phosphorylation of Smad2 and Smad3 as well as a diminished Smad2 and Smad3 gene reporter activity in response to TGF-beta."
"To examine the effect of chronic beta 2 -AR agonist treatment on critical regulators of the myostatin signaling pathway we examined the levels of the Myostatin precursor (pro Myostatin), Smad3, phosphorylated Smad3 relative to Gapdh."
"TGF-beta exerts its pro fibrotic effects through transcription factor signaling Smad3, and selective inhibition of Smad3 phosphorylation and inhibition of Smad3 interaction with Smad4 has been shown to reduce fibroblast activation to the myofibroblast phenotype and also reduce ECM synthetic activity of the cells."
"In the present study, we observed that the TGF-beta1-induced phosphorylation of Smad2 and Smad3 and the TGF-beta1-induced increase in alpha-SMA production were partially dependent on Smad2 and Smad3 and were reversed by knockdown of both Smad2 and Smad3."
"In addition, oridonin reduced the levels of Smad2, Smad3, Smad4, PAI-1 and the phosphorylation of Smad2 and Smad3 induced by TGF-beta1 in vitro."
"To clarify the mechanism by which NDQ relieved renal fibrosis and to verify the systems pharmacology prediction, we detected TGF-beta1, Smad3, phosphorylated Smad3 (p-Smad3), Smad7, p38MAPK, and phosphorylated p38MAPK (p-p38MAPK) by western blotting."
"SMAD-3 mediates EMT in RPE and lens epithelium and inhibition of SMAD-3 phosphorylation can prevent EMT during cataract and PVR [XREF_BIBR]."
"The protein levels of alpha-smooth muscle actin (alpha-SMA), type 1 collagen (Col1), Smad2, Smad3, phosphorylated Smad2 (p-smad2), p-smad3 and Smad7 were assessed by Western blot analysis."
"We then further determined whether resveratrol inhibited TGFbeta and Smad3 stimulated Akt activation by blocking Smad3 phosphorylation."
"Lin et al. (2006) identified protein phosphatase 1A (PP1A)/PP2Calpha as a Smad2 and Smad3 SXS motif specific phosphatase that inhibits Smad2 and Smad3 phosphorylation XREF_BIBR."