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SMAD3 phosphorylates SMAD3. 35 / 35
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"In agreement, pretreatment of MCF-7 cells with 17-beta-estradiol resulted in a reduced phosphorylation of Smad2 and Smad3 as well as a diminished Smad2 and Smad3 gene reporter activity in response to TGF-beta."

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"TGF-beta exerts its pro fibrotic effects through transcription factor signaling Smad3, and selective inhibition of Smad3 phosphorylation and inhibition of Smad3 interaction with Smad4 has been shown to reduce fibroblast activation to the myofibroblast phenotype and also reduce ECM synthetic activity of the cells."

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"In the present study, we observed that the TGF-beta1-induced phosphorylation of Smad2 and Smad3 and the TGF-beta1-induced increase in alpha-SMA production were partially dependent on Smad2 and Smad3 and were reversed by knockdown of both Smad2 and Smad3."

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"Trx overexpression further enhanced Smad3 phosphorylation and TD overexpression decreased Smad3 phosphorylation compared with the Ad-GFP control group after ox-LDL stimulation in HUVECs."

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"In vitro study showed that iTregs could suppress expression of IL-1beta and TNF-alpha, promote transcription of IL-10, and increase phosphorylation of mothers against decapentaplegic homolog 3 (SMAD3) in KCs."

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"Our data showed that both DN-Smad-2 and DN-Smad-3 significantly decreased Smad-2 and Smad-3 phosphorylation (Fig 2, A)."

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"Real time PCR was performed to look for gene expression of Fn and collagen IV and collagen I. TGFbeta1 induced the Smad1/5/8, Smad3 and p38 phosphorylation and CCN3 downregulated the TGFbeta1 induced Smad1/5/8 phosphorylation and did not affect Smad3 and p38 phosphorylation."

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"Second, although a Smad3 specific inhibitor, SIS3, potently inhibited TGFβ-induced Smad3 phosphorylation and subsequent activation of TGFβ/Smad3 dependent gene expression, it did not reverse the inhibitory effect of TGFβ on myogenesis."

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"HF showed a decrease in the TGF-β-induced accumulation of Smad3 and phosphorylated Smad3 (p-Smad3) at 300 nM."

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"In addition, knockdown of SMAD3 almost completely abolished TGF-β1-induced phosphorylation of SMAD3 but not SMAD2 ( Fig. 2 C)."

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"In addition, oridonin reduced the levels of Smad2, Smad3, Smad4, PAI-1 and the phosphorylation of Smad2 and Smad3 induced by TGF-beta1 in vitro."

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"We then further determined whether resveratrol inhibited TGFbeta and Smad3 stimulated Akt activation by blocking Smad3 phosphorylation."

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"Lin et al. (2006) identified protein phosphatase 1A (PP1A)/PP2Calpha as a Smad2 and Smad3 SXS motif specific phosphatase that inhibits Smad2 and Smad3 phosphorylation XREF_BIBR."

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"Sorafenib Inhibits the Expression of Smad3 and Phosphorylated Smad3 in Kidney Tissue and NRK-52E Cells."

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"Furthermore, both SMAD2 and SMAD3 are activated by phosphorylation, and our results support that patients whose TAFs exhibit a low (<1) pSMAD3/pSMAD2 ratio may be refractory to nintedanib and possibly to other antifibrotic drugs, whereas those with high (>1) pSMAD3/pSMAD2 ratio may elicit positive responses (where pSMAD2 and pSMAD3 refers to phosphorylated SMAD2 and SMAD3, respectively) (Figure 3D)."

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"In addition, knockdown of SMAD3 almost completely abolished TGF-beta1-induced phosphorylation of SMAD3 but not SMAD2."

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"Notably, CerS4 knockdown had no significant effect on endogenous Smad7 protein abundance (XREF_FIG, bottom) or canonical TGF-beta signaling compared to Scr shRNA transfected A549 controls, measured by Smad3 dependent luciferase reporter assay (XREF_FIG), and phosphorylation of Smad3 using Western blotting with and without TGF-beta exposure (XREF_FIG)."

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"SIS3 has been demonstrated to reduce TGF-β1-induced Smad3 phosphorylation and interaction of Smad3 with Smad4 [ xref ]."

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"The second scenario is that HEF1 tethers Smad3 in a cytoplasmic complex and the type I receptor activation leads to phosphorylation of Smad3 and the subsequent conformational changes that can enhance its interaction with APC10, which recruits the APC ligase and assists CDH1 to bind the substrate HEF1."

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"Treatment of Smad3 +/+ monocytes with TGF-beta1 induced phosphorylation of Smad3 as well as expression of collagen I, fibronectin, and alpha-SMA."

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"To examine the effect of chronic beta 2 -AR agonist treatment on critical regulators of the myostatin signaling pathway we examined the levels of the Myostatin precursor (pro Myostatin), Smad3, phosphorylated Smad3 relative to Gapdh."

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"To clarify the mechanism by which NDQ relieved renal fibrosis and to verify the systems pharmacology prediction, we detected TGF-beta1, Smad3, phosphorylated Smad3 (p-Smad3), Smad7, p38MAPK, and phosphorylated p38MAPK (p-p38MAPK) by western blotting."

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"We detected the change of Smad3 in anti-CD3/CD28 stimulated splenic CD4+ T cells by western blot and indicated significant upregulation of both total Smad3 and phosphorylated Smad3 in oral atorvastatin treatment group ( ∗ P < 0.05, as in xref ); more interestingly, the knockout of GARP by GARP-siRNA inhibited the upregulation of Smad3 and p-Smad3 induced by atorvastatin ( ∗∗ P < 0.05, as in xref )."

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"Our results further demonstrated that PTX inhibited the effect of TGF-β1 on regulating the expression of Smad3 and phosphorylated Smad3 (p-Smad3), and restored the levels of E-cadherin, vimentin and α-SMA."

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"Smad2, Smad3, phosphorylated Smad3, and Smad4 were detected in all cases."

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"However, neither total protein nor the phosphorylated protein of SMAD3, a major TGFbeta receptor signaling downstream effector in GBM, was affected by DHT, suggesting that AR activation may not affect the SMAD3 protein production or phosphorylation of TGFbeta receptor and SMAD3."

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"We found that KD-HIPK2 inhibited TGFbeta induced Smad3 phosphorylation (XREF_FIG) suggesting that HIPK2 is required for TGFbeta induced Smad3 phosphorylation."

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"We previously found that β2M induced SMAD3 phosphorylation despite an inflammatory phenotype , but SMAD3 must translocate to the nucleus to act as a transcription factor."

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"Protein phosphorylation of SMAD 2 and SMAD 3 as well as SMAD 2, SMAD 3, and SMAD 4 levels was measured using western blot analysis in the DFAT cells incubated with or without (vehicle control) 5ng."

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"SMAD-3 mediates EMT in RPE and lens epithelium and inhibition of SMAD-3 phosphorylation can prevent EMT during cataract and PVR [XREF_BIBR]."

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"The protein levels of alpha-smooth muscle actin (alpha-SMA), type 1 collagen (Col1), Smad2, Smad3, phosphorylated Smad2 (p-smad2), p-smad3 and Smad7 were assessed by Western blot analysis."

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"In normal chondrocytes, mechanotransduction signals induced the translocation of the nuclear factor of activated T-cells 3 (NFAT3) to the nucleus and subsequently activated the expression of miR-140, whereas in OA chondrocytes, overexpression of transforming growth factor-β (TGF-β) resulted in the phosphorylation of mothers against decapentaplegic homolog 3 (SMAD3) that directly inhibited miR-140 [33]."

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"Smad3, COOH-terminally phosphorylated mothers against decapentaplegic homolog 3 (pSmad3), and mothers against decapentaplegic homolog 2 proteins (Smad2) were detected in samples from 5 normal anterior pituitaries and 18 ACTH-PAs by Western blot and immunohistochemical analysis."

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"Collagen-I, collagen-III, fibronectin (FN), alpha-smooth muscle actin (alpha-SMA), TGF-beta1, Smad2, Smad3, phosphorylated (p-) Smad2, p-Smad3 and Smad7 proteins were detected by western blotting as previously described."

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"After PGRN knockdown by siRNA, the expression of PGRN, collagen I (Col I), small mothers against decapentaplegic homolog 3 (Smad3), phosphorylated Smad3 (P-Smad3), transforming growth factor (TGF)-beta1 and TGF-beta receptor I (TbetaRI) were detected by real-time reverse transcription polymerase chain reaction (RT-qPCR) or Western blot."