IndraLab

Statements

USP18 affects MAP3K7
1 | 20
USP18 deubiquitinates MAP3K7. 10 / 20
1 | 19
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"USP18 deubiquitinates TAK1 in a protease dependent manner in HEK293 cells."
33138315
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"In contrast, they suggest that USP18 inhibits ubiquitination of the TAK1 and TAB1 complex in a protease dependent manner XREF_BIBR, XREF_BIBR."
28165509
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"USP18 can inhibit the ubiquitination of the TAK1 and TAB complex, thereby inhibiting IL-2 production and promoting IL-17 production and synthesis."
27809302
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"USP18 directly cleaves the K63 linked polyubiquitin chains, but not K48 linked polyubiquitin chains from TAK1 in a protease dependent manner since the USP18 catalytically inactive mutant can not deubiquitinate TAK1."
26240016
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"Collectively, these data suggest that USP18 targets the TAB1 and TAK1 complex and inhibits TAK1 polyubiquitination modification and kinase activity, thereby restricting TCR mediated NF-kappaB and NFAT activation and subsequent expression of IL-2."
23825189
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"Collectively, our findings revealed that USP18 inhibits TAK1 and NEMO ubiquitination through different mechanisms."
26240016
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"Using similar mechanisms of action, deubiquitination of TAK1 by the deubiquitinase USP18 inhibits TCR (Figure 2)."
| PMC
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"USP18 de-ubiquitinates TAK1, thereby blocking phosphorylation of RCAN1, an inhibitor of calcineurin."
26766444
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"Interestingly, in transfection experiments, Usp18 bound to and deubiquitinated Tak1, thereby reducing its kinase activity and the associated downstream signaling."
29685903
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"Importantly, USP18 is associated with and deubiquitinates the TAK1 and TAB1 complex, thereby restricting expression of IL-2."
23825189
USP18-C61S leads to the deubiquitination of MAP3K7. 1 / 1
| 1
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"In contrast, USP18 (C61S), carrying a single point mutation in the protease domain, could not prevent the polyubiquitination of the TAB1 and TAK1 complex."
23825189
USP18 affects IKBKG
1 | 11
USP18 deubiquitinates IKBKG. 10 / 11
1 | 10
reach
"To investigate the precise mechanisms mediating inhibition of NEMO ubiquitination by USP18, we generated NEMO deletion mutants (XREF_FIG), containing a domain responsible for IKK binding, TANK binding, UBAN, or IkappaBalpha binding."
26240016
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"On the other hand, USP18 inhibits NEMO ubiquitination by directly binding to its UBAN domain."
26240016
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"These results indicate that USP18 inhibits NEMO ubiquitination as well as NF-kappaB activity in a protease independent manner."
26240016
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"USP18 inhibits NEMO ubiquitination at the Lys -325 and 326 sites by masking the UBAN domain of NEMO."
26240016
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"Since the UBAN domain of NEMO contains all five K63 linked ubiquitination sites (Lys 285, Lys 321, Lys 325, Lys 326, and Lys 399) XREF_BIBR and two linear ubiquitination sites for NEMO (Lys 285 and Lys 309) XREF_BIBR, USP18 may prevent NEMO ubiquitination by masking these ubiquitination sites through direct binding."
26240016
reach
"Collectively, our findings revealed that USP18 inhibits TAK1 and NEMO ubiquitination through different mechanisms."
26240016
ubibrowser
"USP18 negatively regulates NF-κB signaling by targeting TAK1 and NEMO for deubiquitination through distinct mechanisms"
26240016
reach
"Another way that USP18 inhibited NF-κB activation is by deubiquitinating K63-Ub of TAK1 and NEMO [42] ."
31208841
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"First, we used the two-step immunoprecipitation assay to assess whether USP18 prevents the conjugated ubiquitination of NEMO."
26240016
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"We next tested whether USP18 inhibited NEMO ubiquitination through USP18 protease activity."
26240016
USP18 leads to the deubiquitination of IKBKG at position 326. 1 / 1
| 1
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"USP18 inhibits NEMO ubiquitination at the Lys -325 and 326 sites by masking the UBAN domain of NEMO."
26240016
USP18 affects TAB1
1 | 6
USP18 deubiquitinates TAB1. 6 / 6
1 | 5
ubibrowser
"USP18 is associated with and deubiquitinates the TAK1-TAB1 complex"
23825189
reach
"Previous studies showed that USP18 potently abolishes the polyubiquitination of TAK1 and TAB1 complex XREF_BIBR."
26240016
reach
"To further examine whether USP18 deubiquitinates the TAK1 and TAB1 complex, we purified Flag tagged USP18 from 293T cells transiently transfected with Flag-USP18 plasmid by immunoprecipitation with anti-Flag agarose and elution with Flag peptide."
23825189
reach
"Importantly, USP18 is associated with and deubiquitinates the TAK1 and TAB1 complex, thereby restricting expression of IL-2."
23825189
reach
"In contrast, they suggest that USP18 inhibits ubiquitination of the TAK1 and TAB1 complex in a protease dependent manner XREF_BIBR, XREF_BIBR."
28165509
reach
"In contrast, they suggest that USP18 inhibits ubiquitination of the TAK1/TAB1 complex in a protease dependent manner30,31."
28165509
USP18-C61S leads to the deubiquitination of TAB1. 1 / 1
| 1
reach
"In contrast, USP18 (C61S), carrying a single point mutation in the protease domain, could not prevent the polyubiquitination of the TAB1 and TAK1 complex."
23825189
USP18 affects STING1
| 4
USP18 deubiquitinates STING1. 4 / 4
| 4
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"USP18 does not deubiquitinate STING in vitro but facilitates USP20 to catalyze deubiquitination of STING in a manner independently of the enzymatic activity of USP18 (91)."
34707613
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"USP18 did not deubiquitinate STING in vitro but facilitated USP20 to catalyze deubiquitination of STING in a manner independent of the enzymatic activity of USP18."
27801882
reach
"Subsequently, they searched for DUBs that interact with USP18 and found that knockdown of USP20 inhibited USP18 induced deubiquitination of STING and knockdown of USP18 inhibited USP20 induced deubiquitination of STING [XREF_BIBR]."
31208841
reach
"These results together supported that although USP18 does not deubiquitinate STING itself, USP18 recruits USP20 to deubiquitinate STING to suppress IFN synthesis [41]."
31208841
USP18 affects TWIST1
| 1 1
USP18 deubiquitinates TWIST1. 2 / 2
| 1 1
trips
"USP18 deubiquitinates and stabilizes Twist1 to promote epithelial-mesenchymal transition in glioblastoma cells."
32368392
reach
"USP18 deubiquitinates and stabilizes Twist1 to promote epithelial-mesenchymal transition in glioblastoma cells."
32368392
USP18 affects TAK1-TAB complexes
| 1
USP18 leads to the deubiquitination of TAK1-TAB complexes. 1 / 1
| 1
reach
"Mechanistically, we found that USP18 interacted with and inhibited ubiquitination and activation of TAK1-TAB complexes."
23825189
USP18 affects TAB1-TAK1
| 1
USP18 deubiquitinates TAB1-TAK1. 1 / 1
| 1
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"We speculate that USP18 might deubiquitinate TAB1-TAK1 directly in a substrate specific manner, which could not be observed in synthesized polyubiquitin chains, or other associated cofactors facilitate this regulation in vivo."
23825189
USP18 affects PTEN
1 |
USP18 deubiquitinates PTEN. 1 / 1
1 |
USP18 affects NR2C2
1 |
USP18 deubiquitinates NR2C2. 1 / 1
1 |
ubibrowser
"USP18 negatively regulates NF-κB signaling by targeting TAK1 and NEMO for deubiquitination through distinct mechanisms"
26240016
USP18 affects NFkappaB
| 1
USP18 leads to the deubiquitination of NFkappaB. 1 / 1
| 1
reach
"These results indicate that USP18 inhibits NEMO ubiquitination as well as NF-kappaB activity in a protease independent manner."
26240016
USP18 affects K285/309R
| 1
USP18 leads to the deubiquitination of K285/309R on K63. 1 / 1
| 1
reach
"To further clarify this point, we constructed NEMO double mutants NEMO (K325/326R) and NEMO (K285/309R) (as control) and found USP18 inhibited K63 linked ubiquitination of the NEMO (WT) and NEMO (K285/309R) constructs, but not the NEMO (K325/326R) mutant (XREF_FIG)."
26240016
USP18 affects ISG15
1 |
USP18 deubiquitinates ISG15. 1 / 1
1 |
ubibrowser
"Prior work implicated the IFN-stimulated gene 15 (ISG15) deubiquitinase (DUB) USP18 as having antineoplastic activity by regulating lung cancer growth and oncoprotein stability."
28242811