IndraLab

Statements

COPS6 affects COPS5
18 3 | 26 43
COPS6 binds COPS5.
18 3 | 25 42
COPS6 binds COPS5. 10 / 71
18 3 | 21 28
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
biogrid
No evidence text available
25144743
sparser
"Interaction of the complex with a NEDDylated CRL leads to a series of conformational change events in Csn2, Csn4 and Csn7, triggering rearrangements in the Csn5–Csn6 dimer, resulting in Csn5 activation by priming the Csn5 JAMM/MPN + motif for deNEDDylation [ xref , xref ]."
33099217
sparser
"In the crystal structure of CSN5-CSN6 heterodimer, initial Ins-1 loop (98–109) conformation blocks the distal ubiquitin binding site which we also observed in cryo-EM structure of Rpn11."
30356695
sparser
"The association of the 1–257 and 31–211 domains of CSN5 and CSN6, amenable to soluble bacterial expression, referred to as CSN5 ΔC and CSN6 Δ , respectively ( xref , xref ) was probed by ITC experiments."
25144743
reach
"These data suggests a hierarchy in the catalytic activity over the enzymatic system and the substrate type, with the inactive CSN5 DeltaC, WT form, CSN5 DeltaC, R106T that has basal activity, the MPN heterodimer alone that has robust isopeptidase activity on non physiological substrates and the CSN5 and CSN6 heterodimer in the context of CSN that recapitulates strong activity on both non physiological and physiological substrates."
25144743
sparser
"This may be the reason behind lower flexibility of Ins-1 loop in the monomeric CSN5 compared to the CSN6 bound CSN5."
30356695
sparser
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation xref ."
25395170
sparser
"Therefore, to characterise further this complex, we probed the association of CSN5 ΔC and CSN6 ΔC by NMR to help define the regions responsible for the interaction."
25144743
reach
"Structural and Biochemical Characterization of the Cop9 Signalosome CSN5 and CSN6 Heterodimer."
25144743
PRSS27 binds COPS6 and COPS5. 3 / 3
| 3
sparser
"The association of CSN5 and CSN6 MPN (for Mpr1/Pad1 N-terminal) domains activates its isopeptidase activity."
25144743
sparser
"Here we report that CSN6 N-terminal domain containing its MPN domain can form a stable heterodimer with CSN5 catalytic domain in vitro ."
25144743
sparser
"CSN5 alone is inactive due to an auto-inhibited conformation of its catalytic domain, and the association of CSN5 with the CSN6 MPN (Mpr1/Pad1 N-terminal) domain activates its isopeptidase activity xref ."
27137867
COPS6 binds COPS5 and MPN domain. 3 / 3
| 3
reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation."
26267535
reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation (Lingaraju et al., 2014)."
26267535
reach
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation 55."
25395170
COPS6 binds COPS5 and CSN3. 3 / 3
| 3
sparser
"Our data also demonstrate that CSNAP binds to CSN3, CSN5 and CSN6, possibly linking the MPN and PCI substructures."
26456823
sparser
"CSNAP interacts with CSN3, CSN5, and CSN6."
26456823
sparser
"We show that CSNAP binds CSN3, CSN5 and CSN6 and its incorporation into the CSN complex is mediated through the C-terminal region involving conserved aromatic residues."
26456823
COPS6 binds COPS5 and GNPTAB. 2 / 2
| 2
sparser
"Sitting atop this platform is the heterodimer formed by the MPN (Mpr1p and Pad1p N terminal) () subunits, CSN5 and CSN6."
26456823
sparser
"Sitting atop this platform is the heterodimer formed by the MPN (Mpr1p and Pad1p N-terminal) ( xref ; xref ; xref ) subunits, CSN5 and CSN6."
26456823
COPS6 binds COPS5 and CRL. 1 / 1
| 1
sparser
"Conformational changes of CSN2, CSN4 and CSN7 provide an induced fit mechanism that propagates the binding of neddylated CRL to the CSN5–CSN6 dimer and activates CSN5 [ xref ]."
29249570
RNF139 binds COPS6 and COPS5. 1 / 1
| 1
sparser
"We previously reported that Drosophila Trc8 genetically and physically interacts with Csn5 and Csn6, but not Rpn8 or Rpn11 ( xref )."
20068067
COPS6 binds COPS5, RPN8, and rpn11. 1 / 1
| 1
sparser
"Considering the Rpn8-Rpn11 and CSN5-CSN6 heterodimers, we found that residues in the distal ubiquitin site are responsible for selectivity and must be taken into consideration for the design of selective inhibitors of CSN5 and Rpn11 in future studies."
30356695
COPS6 binds COPS5 and MPN domains. 1 / 1
| 1
reach
"Having shown that the N-terminal MPN domains of CSN5 and CSN6 heterodimerize, we went on to evaluate the effect of CSN6 DeltaC on CSN5 DeltaC 's affinity for Nedd8 and its isopeptidase activity on substrates ranging from protein to a physiological complex."
25144743
COPS6 binds COPS5 and CSN5i-3. 1 / 1
| 1
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
COPS6 binds COPS5 and CUL1. 1 / 1
| 1
sparser
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose-dependent manner ( xref )."
25395170
PRSS27 binds COPS6, COPS5, and GNPTAB. 1 / 1
| 1
sparser
"Moreover, we demonstrate that CSNAP, which is present at unit stoichiometry, tethers together the two distinct structural elements of the complex by mutually binding the MPN subunits, CSN5 and CSN6, and the PCI subunit CSN3."
26456823
COPS6 activates COPS5.
| 1
COPS6 activates COPS5. 1 / 2
| 1
sparser
"To gain additional insights into the molecular mechanism of CSN5 activation by CSN6, the crystal structure of the MPN − core fragment was determined by molecular replacement at 1.76 Å resolution, using the human Rpn8 ΔC orthologue as the search model (PDB code 2O95; xref )."
25144743
COPS6 inhibits COPS5.
| 1
COPS6 inhibits COPS5. 1 / 1
| 1
reach
"Notably, the MPN domain of CSN6 inhibited the association of CSN5 with Cullin-1, while the C-terminal domain had less impact (XREF_FIG)."
25395170
COPS2 affects COPS3, COPS4, COPS5, COPS6, COPS7A, COPS8, and GPS1
2 |
hprd
No evidence text available
9535219
hprd
No evidence text available
12220626
Rpn11 affects RPN8
| 1
COPS6 binds COPS5, RPN8, and rpn11. 1 / 1
| 1
sparser
"Considering the Rpn8-Rpn11 and CSN5-CSN6 heterodimers, we found that residues in the distal ubiquitin site are responsible for selectivity and must be taken into consideration for the design of selective inhibitors of CSN5 and Rpn11 in future studies."
30356695
RNF139 affects COPS6
| 1
RNF139 binds COPS6 and COPS5. 1 / 1
| 1
sparser
"We previously reported that Drosophila Trc8 genetically and physically interacts with Csn5 and Csn6, but not Rpn8 or Rpn11 ( xref )."
20068067
PRSS27 affects GNPTAB
| 1
PRSS27 binds COPS6, COPS5, and GNPTAB. 1 / 1
| 1
sparser
"Moreover, we demonstrate that CSNAP, which is present at unit stoichiometry, tethers together the two distinct structural elements of the complex by mutually binding the MPN subunits, CSN5 and CSN6, and the PCI subunit CSN3."
26456823
MPN domains affects COPS6
| 1
COPS6 binds COPS5 and MPN domains. 1 / 1
| 1
reach
"Having shown that the N-terminal MPN domains of CSN5 and CSN6 heterodimerize, we went on to evaluate the effect of CSN6 DeltaC on CSN5 DeltaC 's affinity for Nedd8 and its isopeptidase activity on substrates ranging from protein to a physiological complex."
25144743
GNPTAB affects PRSS27
| 1
PRSS27 binds COPS6, COPS5, and GNPTAB. 1 / 1
| 1
sparser
"Moreover, we demonstrate that CSNAP, which is present at unit stoichiometry, tethers together the two distinct structural elements of the complex by mutually binding the MPN subunits, CSN5 and CSN6, and the PCI subunit CSN3."
26456823
CUL1 affects COPS6
| 1
COPS6 binds COPS5 and CUL1. 1 / 1
| 1
sparser
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose-dependent manner ( xref )."
25395170
CSN5i-3 affects COPS6
| 1
COPS6 binds COPS5 and CSN5i-3. 1 / 1
| 1
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
CRL affects COPS6
| 1
COPS6 binds COPS5 and CRL. 1 / 1
| 1
sparser
"Conformational changes of CSN2, CSN4 and CSN7 provide an induced fit mechanism that propagates the binding of neddylated CRL to the CSN5–CSN6 dimer and activates CSN5 [ xref ]."
29249570
COPS6 affects rpn11
| 1
COPS6 binds COPS5, RPN8, and rpn11. 1 / 1
| 1
sparser
"Considering the Rpn8-Rpn11 and CSN5-CSN6 heterodimers, we found that residues in the distal ubiquitin site are responsible for selectivity and must be taken into consideration for the design of selective inhibitors of CSN5 and Rpn11 in future studies."
30356695
COPS6 affects RNF139
| 1
RNF139 binds COPS6 and COPS5. 1 / 1
| 1
sparser
"We previously reported that Drosophila Trc8 genetically and physically interacts with Csn5 and Csn6, but not Rpn8 or Rpn11 ( xref )."
20068067
COPS6 affects MPN domains
| 1
COPS6 binds COPS5 and MPN domains. 1 / 1
| 1
reach
"Having shown that the N-terminal MPN domains of CSN5 and CSN6 heterodimerize, we went on to evaluate the effect of CSN6 DeltaC on CSN5 DeltaC 's affinity for Nedd8 and its isopeptidase activity on substrates ranging from protein to a physiological complex."
25144743
COPS6 affects CUL1
| 1
COPS6 binds COPS5 and CUL1. 1 / 1
| 1
sparser
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose-dependent manner ( xref )."
25395170
COPS6 affects CSN5i-3
| 1
COPS6 binds COPS5 and CSN5i-3. 1 / 1
| 1
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
COPS6 affects CRL
| 1
COPS6 binds COPS5 and CRL. 1 / 1
| 1
sparser
"Conformational changes of CSN2, CSN4 and CSN7 provide an induced fit mechanism that propagates the binding of neddylated CRL to the CSN5–CSN6 dimer and activates CSN5 [ xref ]."
29249570
COPS5 affects rpn11
| 1
COPS6 binds COPS5, RPN8, and rpn11. 1 / 1
| 1
sparser
"Considering the Rpn8-Rpn11 and CSN5-CSN6 heterodimers, we found that residues in the distal ubiquitin site are responsible for selectivity and must be taken into consideration for the design of selective inhibitors of CSN5 and Rpn11 in future studies."
30356695
COPS5 affects RNF139
| 1
RNF139 binds COPS6 and COPS5. 1 / 1
| 1
sparser
"We previously reported that Drosophila Trc8 genetically and physically interacts with Csn5 and Csn6, but not Rpn8 or Rpn11 ( xref )."
20068067
COPS5 affects MPN domains
| 1
COPS6 binds COPS5 and MPN domains. 1 / 1
| 1
reach
"Having shown that the N-terminal MPN domains of CSN5 and CSN6 heterodimerize, we went on to evaluate the effect of CSN6 DeltaC on CSN5 DeltaC 's affinity for Nedd8 and its isopeptidase activity on substrates ranging from protein to a physiological complex."
25144743
COPS5 affects CUL1
| 1
COPS6 binds COPS5 and CUL1. 1 / 1
| 1
sparser
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose-dependent manner ( xref )."
25395170
COPS5 affects CSN5i-3
| 1
COPS6 binds COPS5 and CSN5i-3. 1 / 1
| 1
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
COPS5 affects CRL
| 1
COPS6 binds COPS5 and CRL. 1 / 1
| 1
sparser
"Conformational changes of CSN2, CSN4 and CSN7 provide an induced fit mechanism that propagates the binding of neddylated CRL to the CSN5–CSN6 dimer and activates CSN5 [ xref ]."
29249570
COPS5 affects COPS6, RPN8, and rpn11
| 1
COPS6 binds COPS5, RPN8, and rpn11. 1 / 1
| 1
sparser
"Considering the Rpn8-Rpn11 and CSN5-CSN6 heterodimers, we found that residues in the distal ubiquitin site are responsible for selectivity and must be taken into consideration for the design of selective inhibitors of CSN5 and Rpn11 in future studies."
30356695
COPS5 affects COPS6, and RNF139
| 1
RNF139 binds COPS6 and COPS5. 1 / 1
| 1
sparser
"We previously reported that Drosophila Trc8 genetically and physically interacts with Csn5 and Csn6, but not Rpn8 or Rpn11 ( xref )."
20068067
COPS5 affects COPS6, and MPN domains
| 1
COPS6 binds COPS5 and MPN domains. 1 / 1
| 1
reach
"Having shown that the N-terminal MPN domains of CSN5 and CSN6 heterodimerize, we went on to evaluate the effect of CSN6 DeltaC on CSN5 DeltaC 's affinity for Nedd8 and its isopeptidase activity on substrates ranging from protein to a physiological complex."
25144743
COPS5 affects COPS6, GNPTAB, and PRSS27
| 1
PRSS27 binds COPS6, COPS5, and GNPTAB. 1 / 1
| 1
sparser
"Moreover, we demonstrate that CSNAP, which is present at unit stoichiometry, tethers together the two distinct structural elements of the complex by mutually binding the MPN subunits, CSN5 and CSN6, and the PCI subunit CSN3."
26456823
COPS5 affects COPS6, and CUL1
| 1
COPS6 binds COPS5 and CUL1. 1 / 1
| 1
sparser
"We found that both CSN6 and CSN5 associated with Cullin-1 and that CSN6 and CSN5 competed with each other for Cullin-1 binding in a dose-dependent manner ( xref )."
25395170
COPS5 affects COPS6, and CSN5i-3
| 1
COPS6 binds COPS5 and CSN5i-3. 1 / 1
| 1
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
COPS5 affects COPS6, and CRL
| 1
COPS6 binds COPS5 and CRL. 1 / 1
| 1
sparser
"Conformational changes of CSN2, CSN4 and CSN7 provide an induced fit mechanism that propagates the binding of neddylated CRL to the CSN5–CSN6 dimer and activates CSN5 [ xref ]."
29249570
COPS2 affects COPS3, COPS4, COPS5, COPS6, COPS7A, COPS8, GPS1, and TP53
1 |
TP53 binds COPS4, COPS7A, COPS6, COPS3, COPS5, COPS8, COPS2, and GPS1. 1 / 1
1 |
hprd
No evidence text available
11285227
COPS2 affects COPS3, COPS4, COPS5, COPS6, COPS7A, COPS8, CUL4A, and GPS1
1 |
hprd
No evidence text available
11337588
hprd
No evidence text available
11337588
SKP1 binds FBXO3, FBXO6, COPS4, COPS7A, COPS7B, COPS6, COPS3, COPS5, COPS8, CUL1, COPS2, EEF2, GPS1, and RBX1. 1 / 1
1 |
hprd
No evidence text available
11337588