IndraLab

Statements

COPS6 binds COPS5. 70 / 71
18 3 | 21 28
sparser
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
biogrid
No evidence text available
25144743
sparser
"Interaction of the complex with a NEDDylated CRL leads to a series of conformational change events in Csn2, Csn4 and Csn7, triggering rearrangements in the Csn5–Csn6 dimer, resulting in Csn5 activation by priming the Csn5 JAMM/MPN + motif for deNEDDylation [ xref , xref ]."
33099217
sparser
"In the crystal structure of CSN5-CSN6 heterodimer, initial Ins-1 loop (98–109) conformation blocks the distal ubiquitin binding site which we also observed in cryo-EM structure of Rpn11."
30356695
sparser
"The association of the 1–257 and 31–211 domains of CSN5 and CSN6, amenable to soluble bacterial expression, referred to as CSN5 ΔC and CSN6 Δ , respectively ( xref , xref ) was probed by ITC experiments."
25144743
reach
"These data suggests a hierarchy in the catalytic activity over the enzymatic system and the substrate type, with the inactive CSN5 DeltaC, WT form, CSN5 DeltaC, R106T that has basal activity, the MPN heterodimer alone that has robust isopeptidase activity on non physiological substrates and the CSN5 and CSN6 heterodimer in the context of CSN that recapitulates strong activity on both non physiological and physiological substrates."
25144743
sparser
"This may be the reason behind lower flexibility of Ins-1 loop in the monomeric CSN5 compared to the CSN6 bound CSN5."
30356695
sparser
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain and the dimer is topologically knotted to engage in Cullin deneddylation xref ."
25395170
sparser
"Therefore, to characterise further this complex, we probed the association of CSN5 ΔC and CSN6 ΔC by NMR to help define the regions responsible for the interaction."
25144743
reach
"Structural and Biochemical Characterization of the Cop9 Signalosome CSN5 and CSN6 Heterodimer."
25144743
reach
"Structural data provide evidence for coordinated domain changes of CSN2, CSN4, and CSN7 and the CSN5-CSN6 heterodimer induced by neddylated CRL4A converting CSN5 into its active conformation [37]."
32708147
biogrid
No evidence text available
21145461
reach
"In the CSN5-CSN6 heterodimer we observed that CSN5i-3 is stable (Figure 12C) and its conformation is very close to the conformation reported in the crystal structure (Figure S6)."
30356695
reach
"Moreover, in the CSN5 and CSN6 heterodimer, alpha4 helix of CSN6, a C-terminal extension of the MPN domain, interacts with alpha6 helix of CSN5."
25242525
biogrid
No evidence text available
22939629
sparser
"Interestingly, STAMBPL1 has a direct interaction with the constitutive photomorphogenic 9 (COP9 or CSN) signalosome subunits CSN5 and CSN6."
35066747
reach
"This may be the reason behind lower flexibility of Ins-1 loop in the monomeric CSN5 compared to the CSN6 bound CSN5."
30356695
hprd
No evidence text available
11337588
reach
"In the crystal structure of CSN5-CSN6 heterodimer, initial Ins-1 loop (98–109) conformation blocks the distal ubiquitin binding site which we also observed in cryo-EM structure of Rpn11."
30356695
sparser
"This analysis could strengthen the view that CSN5 ΔC and CSN6 ΔC interact through an architecture reminiscent of the Rpn11 ΔC /Rpn8 ΔC complex."
25144743
biogrid
No evidence text available
19295130
sparser
"The above suggests that the MPN dimer (CSN5–CSN6 dimer) could be highly conserved."
33806190
biogrid
No evidence text available
19615732
biogrid
No evidence text available
19295130
biogrid
No evidence text available
28514442
hprd
No evidence text available
11285227
sparser
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation ()."
26267535
reach
"Binding of capzimin and CSN5i-3 to monomeric CSN5 and CSN5-CSN6 heterodimer."
30356695
biogrid
No evidence text available
19615732
biogrid
No evidence text available
21145461
sparser
"MD simulations studies and binding energy analysis revealed that the selective binding of the inhibitors can be only explained by the consideration of larger heterodimeric complexes of Rpn11 (Rpn8-Rpn11) and CSN5 (CSN5-CSN6)."
30356695
sparser
"Previous structural studies of the CSN suggested that CSN5 and CSN6 interact xref , xref ."
25144743
sparser
"MD simulation of capzimin with monomeric CSN5 and CSN5-CSN6 heterodimer revealed that its binding to CSN5 is also influenced by the CSN6 (Figure xref )."
30356695
sparser
"The cap sits at the top of the box and consists of a CSN5–CSN6 heterodimer ( xref ) ( xref )."
28271482
biogrid
No evidence text available
19141280
biogrid
No evidence text available
27173435
reach
"CSN5 and CSN6, which have an MPN (Mpr1 and Pad1 N-terminal) domain, form a dimer and are embedded at the core of the helical bundle."
29616013
reach
"In analogy to the CSN5-CSN6 heterodimer, RPN11 partners with another MPN domain protein, RPN8, possessing an inactive JAMM domain."
32708147
biogrid
No evidence text available
18850735
reach
"In our CSN6 homodimer structure, alpha1 and alpha2 from one monomer interact with alpha2 and alpha1 from another monomer, respectively, while in the CSN5 and CSN6 heterodimer, alpha1 and alpha2 helice[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25242525
biogrid
No evidence text available
26186194
sparser
"In analogy to the CSN5-CSN6 heterodimer, RPN11 partners with another MPN domain protein, RPN8, possessing an inactive JAMM domain."
32708147
reach
"The structure of the CSN5-CSN6 heterodimer (Figure S3A) was extracted from the crystal structure of human COP9 signalasome (PDB_code: 4D10) (Lingaraju et al., 2014) C-terminal regions of CSN5 (258–333) and CSN6 (215–316) were also deleted since they are not involved in catalysis and heterodimer formation can take place without them."
30356695
biogrid
No evidence text available
22863883
reach
"The processed CSN5-CSN6 heterodimer is shown in Figure 3B and Figure S3C."
30356695
sparser
"Exploiting the above detailed finding that CSN5 ΔC and Nedd8 interaction proceeds through similar surfaces to that of AMSH-LP-distal ubiquitin's ( xref , xref ), a complex composed of CSN5 ΔC displaying an AMSH-LP-like Ins-1 conformation and of Nedd8 was assembled and used to probe the association of CSN5 ΔC and CSN6 ΔC ."
25144743
sparser
"Rpn8-RPN11 and CSN5-CSN6 heterodimeric states are shown in Figure xref ."
30356695
sparser
"In the CSN5-CSN6 heterodimer we observed that CSN5i-3 is stable (Figure xref ) and its conformation is very close to the conformation reported in the crystal structure (Figure xref )."
30356695
reach
"MD simulation of capzimin with monomeric CSN5 and CSN5-CSN6 heterodimer revealed that its binding to CSN5 is also influenced by the CSN6 (Figure 10)."
30356695
biogrid
No evidence text available
25144743
reach
"These structures suggest a conserved mechanism of CSN activation, consisting of conformational clamping of the CRL2 substrate by CSN2 and CSN4, release of the catalytic CSN5 and CSN6 heterodimer and finally activation of the CSN5 deneddylation machinery."
31444342
hprd
No evidence text available
12220626
sparser
"However, in the CSN5-CSN6 heterodimer, CSN5i-3 forms stable H-bonds with both Thr154 and Asn158 (Figure xref and Figure xref )."
30356695
sparser
"Structural data provide evidence for coordinated domain changes of CSN2, CSN4, and CSN7 and the CSN5-CSN6 heterodimer induced by neddylated CRL4A converting CSN5 into its active conformation [ xref ]."
32708147
sparser
"The MPN domains of CSN5 and CSN6 form a stable heterodimer."
25144743
reach
"Alternatively, based on recent finding on the structure of eight-unit COP9 complex XREF_BIBR, it is possible to modulate COPS5 activity by tackling the interactions between COP9 subunits, particularly the interaction between COPS5 and COPS6 and CSN6 subunits that is crucial for maintaining the isopeptidase activity."
27375289
reach
"38 Also the MPN domain is involved in heterodimerization between CSN6 and CSN5 to regulate Cullin neddylation."
33101846
reach
"However, in the CSN5-CSN6 heterodimer, CSN5i-3 forms stable H-bonds with both Thr154 and Asn158 (Figure S5D and Figure 13B)."
30356695
sparser
"For instance, in plant cells, isoforms have been identified for the MPN‐domain subunits CSN5 (CSN5A and CSN5B) and CSN6 (CSN6A and CSN6B) which form four distinct CSN variants."
27833851
sparser
"It is noteworthy that, although the interaction between CSN5 ΔC and CSN6 ΔC was impaired by the H44A or to a stronger extent by the V115A substitution, the effect of these variants remains modest (2 to 5-fold)."
25144743
sparser
"Structurally, CSN6 and CSN5 form a heterodimer through MPN domain, and the dimer is topologically knotted to engage in Cullin deneddylation ( xref )."
26267535
sparser
"Considering the Rpn8-Rpn11 and CSN5-CSN6 heterodimers, we found that residues in the distal ubiquitin site are responsible for selectivity and must be taken into consideration for the design of selective inhibitors of CSN5 and Rpn11 in future studies."
30356695
biogrid
No evidence text available
26344197
reach
"However, in the CSN5-CSN6 heterodimer, capzimin displayed a monodentate coordination with Zn and only low occupancy H-bonds with side chains of Met78, Arg106, and Asn158 (Figure S5B)."
30356695
biogrid
No evidence text available
21145461
sparser
"However, in the CSN5-CSN6 heterodimer, capzimin displayed a monodentate coordination with Zn 2+ and only low occupancy H-bonds with side chains of Met78, Arg106, and Asn158 (Figure xref )."
30356695
sparser
"Sitting atop…is the CSN5–CSN6 tomato….” [ xref ])."
31195722
reach
"First, the catalytic activity efficiency can be achieved by an increase of affinity for Nedd8 observed either in the CSN5 and CSN6 complex or by the conformational relaxation of the Ins-1 segment."
25144743
reach
"The MPN domains of the CSN5 and CSN6 heterodimer rest on the helical bundle while their carboxy terminal helical tails are inserted into the helical bundle."
26437438
biogrid
No evidence text available
21625211