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OTUD3 is acetylated on K129. 14 / 14
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"Although we have not systematically investigated the stoichiometry, we found that Lys129 acetylation of OTUD3 occurred more efficiently in innate immune cells and lymphocytes."
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"To confirm that OTUD3 was acetylated at Lys129, we generated an antibody specific for Lys129-acetylated OTUD3."
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"Intriguingly, OTUD1 with a corresponding amino acid, Gln, possesses a very strong capacity to cleave Lys63-linked Ub chain, while OTUD2 with a corresponding amino acid, Arg, shows absolutely no affini[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To unambiguously determine the effect of acetylation on OTUD3, we utilized genetic code expansion orthogonal systems ( Neumann et al., 2008 ; Ryu and Schultz, 2006 ) to incorporate acetyl-lysine into [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To confirm this, acetyl‐lysine is incorporated into a recombinant OTUD3 to generate a fully Lys129‐acetylated OTUD3 (OTUD3 Lys129‐Ac ) with chemical biology approaches."
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"As expected, antibodies specific for Lys129-acetylated OTUD3 could detect OTUD3 Lys129-Ac but not OTUD3 Non-Ac ( Figure 5 K)."
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"We found that OTUD3 Lys129-Ac was rather efficient in an Ub-AMC assay, while the OTUD3 Non-Ac was much less active in cleaving Ub-AMC in vitro ( Figure 5 L), indicating the Lys129-acetylated OTUD3 is [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We found that cells treated with sirtuin inhibitor NAM, but not the HDAC inhibitor Trichostatin A (TSA), exhibited increased Lys129 acetylation of endogenous OTUD3 ( Figure 6 A)."
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"OTUD3 that is acetylated at Lys129 (acetyl-Lys129 OTUD3) shows markedly enhanced affinity to Lys63 linkage on MAVS."
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"NAM treatment also elevated Lys129 acetylation of ectopic expressed OTUD3 and nearly completely blocked virus-induced deacetylation of OTUD3 ( Figure 6 B), indicating sirtuins might be involved in dea[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Remarkably, acetylation of OTUD3 on lysine 129 (K129) triggers deubiquitination of K63-polyubiquitination chains on MAVS thereby blocking the antiviral response ( xref ) ( xref B )."
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"SeV infection reduced Lys129 acetylation of OTUD3 in control cells, but not in cells depleted with SIRT1 ( Figure 6 G), indicating that endogenous SIRT1 is required."
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"Compared to the control, cells treated with EX527 exhibited increased Lys129 acetylation of endogenous OTUD3 and became resistant to virus-induced deacetylation ( Figure 6 H), showing that endogenous [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To understand the functional importance of acetylation on OTUD3 Lys129, we used the CRISPR-Cas9 system to create mutations resulting in the Lys129R substitution, OTUD3 (Lys129R), and Lys129Gln substit[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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