The INDRA Database

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"Our data suggest that inhibiting Thr122 phosphorylation of USP13 may provide an effective therapeutic strategy for targeting USP13 in USP13-amplified ovarian cancer."

36612196

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"To understand the biological role of Thr122 phosphorylation of USP13 in ovarian cancer, we examined the effect of inhibiting T122 phosphorylation on cell proliferation."

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"This study revealed that USP13 is phosphorylated at Thr122, which upregulates the stability of USP13 at a post-translation level."

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"Here, we found that USP13 is phosphorylated at Thr122 in ovarian cancer cells."

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"We also reveal that phosphorylation at Threonine 122 of USP13 promotes the proliferation of ovarian cancer cells."

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"Inhibition of CK2 Decreased Thr122 Phosphorylation on USP13."

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"To study the function of phosphorylation on Thr122 of USP13 in ovarian cancer, we generated an anti-phospho-Thr122 USP13 antibody (anti-pT122 antibody)."

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"The treatment with CK2 inhibitor (CX-4945) dramatically reduced the signal of Thr122 phosphorylation of USP13 in HEK293T cells ( xref A)."

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"To validate the activity of the anti-pT122 antibody, we generated the point mutation of Thr122 by using a site-directed substitution of threonine to alanine on USP13 to abolish USP13 phosphorylation on Thr122 residue."

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"The result indicated significant inhibition of USP13 phosphorylation on Thr122 at a concentration from 1 µM of CX-4945."

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"Peptide competition assay further validated the specificity of this antibody against USP13 phosphorylation at Thr122 ( xref A)."

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"The phosphorylation at Thr122 of USP13 could result in various effects on USP13."