IndraLab

Statements

USP15 binds MYC. 9 / 9
| 9
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"HEK293T cells were co-transfected with plasmid encoding HA–RIG-I, Flag-IPS-1, increasing amounts of wild-type Myc–USP15 or its mutants."
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"To determine the precise role of USP15 plays in mediating the IFN signaling pathway and figure out the factors that drive the main different results, we asked for the USP15-Myc and TRIM25-V5 plasmids from Dr."
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"In contrast, poly(I:C)-induced inhibition of VSV replication was recovered by overexpression of both USP15-HA and USP15-Myc (Supplementary Fig. S5)."
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"As shown in supplementary Fig. S5, neither USP15-HA nor USP15-Myc suppressed the replication of VSV, not in accordance with the result described in Pauli’s paper that USP15 limited the VSV replication."
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"However, we also found that co-expression of increasing amounts of USP15-HA and USP15-Myc further enhanced the IFN-β promoter activation stimulated by RIG-I-N and TRIM25 as a function of the dose, in line with the result described by Pauli (Supplementary Fig. S5)."
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"We found both USP15-HA and USP15-Myc dose-dependently inhibited the SEV- and RIG-I-N-induced activation of IFN-β (Supplementary Fig. S5)."
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"Ectopically expressed Myc-USP15 was readily detected in Flag-TET2 immune complex ( xref )."
| PMC
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"Scale bar, 10 μm. (D) Quantification of the percentage of Parkin-positive cells (in the conditions transfected with Parkin and EV) or Parkin- and Myc-positive cells (in the conditions transfected with Parkin and USP15-Myc) in which Parkin colocalized with mitochondria (n = 3). (E) HEK293 cells were transfected with EV or Myc-tagged USP15 and treated with DMSO or CCCP (10 µm) for 3 h."
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"Scale bar, 10 mm. (D) Quantification of the percentage of Parkin-positive cells (in the conditions transfected with Parkin and EV) or Parkin-and Myc-positive cells (in the conditions transfected with Parkin and USP15-Myc) in which Parkin colocalized with mitochondria (n ¼ 3). (E) HEK293 cells were transfected with EV or Myctagged USP15 and treated with DMSO or CCCP (10 mM) for 3 h."
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