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USP13 increases the amount of ZHX2. 5 / 5
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"Among them, we found that depletion of USP13, but not the other DUBs, down-regulated ZHX2 protein levels in ccRCC cell lines (UMRC-2) (Fig. 1B)."
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"First, we overexpressed USP13 in 293T cells and found that USP13 led to increased ZHX2 protein levels in 293T cells or HKC cells while VHL overexpression caused down-regulation of ZHX2 (Fig. 1 G and H), reinforcing the role of pVHL as the E3 ligase for ZHX2 as shown previously (4)."
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"WT, but not catalytically inactive USP13 (CD), up-regulated ZHX2 protein levels (Fig.1 I and J), revealing that USP13 overexpression leads to the up-regulation of the ZHX2 protein level in an enzymatically dependent way."
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"Accordingly, USP13 depletion by single guide RNAs down-regulated ZHX2 protein levels in 786-O, the phenotype ameliorated by concurrent treatment with the proteasomal inhibitor MG132 (Fig. 2E)."
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"In conclusion, our results suggest that USP13 depletion down-regulated the ZHX2 level by increasing ZHX2 ubiquitination and degradation."
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