IndraLab

Statements

OTUD4 binds FlaG. 4 / 4
| 4
sparser
"We applied UV crosslinking to stabilize RNA–protein interactions, immunoprecipitated FLAG–OTUD4 and subsequently isolated the co-precipitated RNA from OTUD4 or control beads."
31138677
sparser
"Notably, when using full-length FLAG-OTUD4 proteins affinity purified from HEK293T cells, H148Y unexpectedly cleaved K48-linked Ub 2–7 at WT levels; H148A and the active-site mutant C45A also showed K48 cleavage comparable to WT in both time-course and dose–response assays ( xref , D and E )."
41062071
sparser
"To elucidate whether the OTUD4 granules also contained RNA, we carried out in situ hybridization with a fluorescently (Cy3)-labeled oligo(dT) probe to detect poly(A)-tails of mRNAs ( xref B), together with immunostaining for FLAG–OTUD4."
31138677
sparser
"These RNA-IPs showed a clear enrichment of RNA with FLAG–OTUD4 compared to that seen in control IPs ( xref C)."
31138677