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"First, we examined the mechanism through which FBXO31 regulated the stability of β-catenin, a key mediator of the Wnt signaling pathway xref and bone formation during osteogenic differentiation xref , using co-IP and western blot assays with wild-type FBXO31 and myc-FBXO31ΔF. As shown in Fig. xref , FBXO31 specifically interacted with β-catenin, Skp1, and USP53."