IndraLab

Statements

OTUB1 is methylated. 21 / 21
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"Another research confirmed that SETD7-mediated OTUB1 methylation promotes ferroptosis by alleviating OTUB1-dependent ferroptosis suppression [ xref ]."
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"Thus, we sought to know whether SET7-mediated methylation of OTUB1 can affect OTUB1 binding to the E2 enzyme, UBC13."
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"These data suggest that SET7-mediated methylation of OTUB1 attenuates OTUB1 binding to UBC13."
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"However, the methylated OTUB1 was not detected when Flag-tagged OTUB1-K122A (Flag-OTUB1-K122A) was cotransfected with SET7 or its enzymatically deficient mutant (SET7-H297A) in OTUB1 −/− H1299 cells ( xref E )."
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"SET7-mediated methylation of OTUB1 attenuates OTUB1 binding to UBC13."
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"However, reconstitution of Flag-tagged WT OTUB1 in OTUB1 −/− H1299 cells restored the methylated OTUB1 to be detected ( xref D )."
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"By contrast, reconstitution of methylation-site mutant of OTUB1 (OTUB1-K122A) could not restore the methylated OTUB1 ( xref D )."
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"SET7-mediated methylation of OTUB1 relieves the suppressive role of OTUB1 on ferroptosis."
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"Since OTUB1 possesses a noncanonical function independently of its DUB activity by binding to and inhibiting the E2 ubiquitin-conjugation enzymes, such as UBC13 ( xref , xref , xref ), SET7-mediated methylation of OTUB1 attenuates OTUB1 binding to UBC13."
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"Therefore, we sought to determine whether SET7-mediated methylation of OTUB1 has an impact on ferroptosis."
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"To further determine whether endogenous OTUB1 was methylated by SET7, we examined methylation of OTUB1 in SET7 +/+ and SET7 −/− H1299 cells after coimmunoprecipitation with anti-OTUB1 antibody."
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"In addition, methylation of OTUB1 was induced by Erastin treatment ( xref J )."
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"Finally, we validated the role of SET7-mediated methylation of OTUB1 in ferroptosis."
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"Collectively, these data suggest that SET7-mediated methylation of OTUB1 relieves its suppressive role on ferroptosis induced by various stress."
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"Moreover, we found using cell viability analysis and intracellular reactive oxygen species assay that SET7-mediated methylation of OTUB1 relieves its suppressive role on ferroptosis."
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"SET7-mediated methylation of OTUB1 does not affect enzymatic activity, protein stability, and subcellular location of OTUB1."
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"MCAO/R rat brains and OGD/R cells showed decreased FTO expression and increased OTUB1 m6A methylation."
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"These data suggest that SET7-mediated methylation of OTUB1 does not affect deubiquitinase activity of OTUB1."
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"For example, the methylation of OTUB1 compromises its binding with UBC13 (an E2-conjugating enzyme), eventually abolishing the suppressive role of OTUB1 in ferroptosis."
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"Further, SET7-mediated methylation of OTUB1 alleviates the inhibitory effect of OTUB1 on ferroptosis and relieves its effect on erastin-induced intracellular ROS production xref ."
40959280
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"These data suggest that SET7-mediated methylation of OTUB1 does not affect the subcellular localization of OTUB1."
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