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UBE2E1 activates OTUB1. 2 / 2

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"A comparison of the effect of several different E2 enzymes on OTUB1 DUB activity showed that the UBE2D enzymes and UBE2E1 robustly stimulate OTUB1, although UBE2N only weakly enhanced cleavage of K48 diubiquitin under the conditions tested 37."

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"UBE2E1 protein levels could also be restored in OTUB1 CRISPR knockout cell lines by expressing either FLAG-OTUB1 or untagged OTUB1 (Fig. 2D)."

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UBE2E1 activates OTUB1. 2 / 2

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