IndraLab

Statements

ASHR3 binds CALM1. 5 / 5
| 5
sparser
"CAM-SML (0.5 mg) was deglycosylated with glycosidase F (5 mU) in 0.5 ml 20 mM Tris–HCl buffer (pH 8.0) at 37 °C for 16 h."
17184920
sparser
"CAM-SML was digested with Achromobacter protease I and subjected to ConA-Sepharose chromatography which could bind mannose-containing sugar chains specifically."
17184920
sparser
"The digest with Achromobacter protease I of CAM-SML was applied to a ConA-Sepharose column (5 × 35 mm, 0.8 ml) pre-equilibrated with 20 mM Tris–HCl buffer (pH 8.0) containing 0.15 M NaCl and 1 mM CaCl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17184920
sparser
"The deglycosylated CAM-SML was digested with endoproteinase Arg-C (5 μg, S / E = 100:1) in 40 μl 0.1 M Tris–HCl buffer (pH 7.6) containing 10 mM CaCl 2 at 37 °C for 16 h."
17184920
sparser
"The enzymatic digests of CAM-SML with Achromobacter protease I and S. aureus V8 protease were separated to peptides, designated K1-K5 and V1-V7, respectively, by RP-HPLC as shown in Fig. 3 A and B . D[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17184920