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OTUB2 binds MYC. 11 / 11
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"The same conclusions were obtained from a proximity ligation assay (PLA) in ES-2 and A2780 cells stably expressing Myc-OTUB2 and Flag-SNX29P2 ( xref )."
38701117
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"RD21A‐FLAG exhibited increased accumulation when co‐expressed with OTU2‐Myc compared to the control (Figure  xref )."
40223806
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"To further elucidate the role of OTU2 in stabilizing RD21A, we co‐expressed RD21A‐FLAG with OTU2‐Myc or an empty vector in N. benthamiana leaves."
40223806
biogrid
No evidence text available
35217790
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"Notably, the presence of OTU2‐Myc suppressed the co‐precipitation of RD21A‐FLAG with PbE3‐2‐GFP, suggesting that OTU2 interferes with the interaction between PbE3‐2 and RD21A in plants (Figure  xref )."
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"Given that OTU2 is a DUB, we hypothesized that it may remove ubiquitin modifications from RD21A. To test this, Ub‐HA, PbE3‐2‐GFP and RD21A‐FLAG were co‐expressed with either OTU2‐Myc or Ev‐Myc in N. benthamiana leaves."
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"Transient expression of PbE3‐2‐GFP, RD21A‐FLAG and OTU2‐Myc in N. benthamiana revealed that OTU2 increased RD21A accumulation compared to the control with an empty vector (Figure  xref ), suggesting that OTU2 may protect RD21A from degradation mediated by PbE3‐2."
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"Similarly, mRD21A also showed enhanced protein accumulation in the presence of OTU2‐Myc (Figure  xref )."
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"To validate the existence of TFs that mediate the activation of CALML3 by OTUB2, immunoprecipitation (IP) experiments in KYSE30 and UM1 cells expressing Myc-OTUB2 proteins were performed using anti-My[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36288705
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"Since our previous data indicated that the tumor-suppressive role of OTUB2 relies on its enzymatic activity, an IP group using cells expressing Myc-OTUB2 C51S was included to exclude proteins whose in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36288705
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"In Co‐IP assays, RD21A‐FLAG and PbE3‐2‐GFP were co‐expressed with either OTU2‐Myc or Ev‐Myc in N. benthamiana leaves."
40223806