IndraLab

Statements

OTUD5 binds Flag. 10 / 10
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"In ubiquitination experiments, we transfected 3 µg His‐α‐Syn, 2 µg Flag‐OTUD5 or its mutant and 1 µg HA‐ubiquitin."
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"To validate which type of MyD88 polyubiquitin chain was abrogated by OTUD5, HA-ubiquitin mutants K0, K6, K11, K27, K29, K33, K48 and K63 (containing only one lysine residue with other lysine residues replaced by arginine), together with Myc-MyD88 and Flag-OTUD5 (WT) were co-transfected into HEK293T cells."
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"Each myc-tagged PDCD5 point mutant was co-transfected with Flag-p53 or Flag-OTUD5, and then each PDCD5 mutant was screened by immunoprecipitation analysis."
25499082
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"Moreover, Flag-OTUD5 and His-TAK1 plasmids were co-transfected into NIH/3T3 cells, confirming the exogenous interaction between OTUD5 and TAK1 (Fig.  xref )."
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"To investigate whether ubiquitination was involved in MyD88 oligomerization, we transfected Flag-OTUD5 (WT) or its mutant C224S and GFP-MyD88 into HEK293T cells."
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"The results showed that transfection of Flag-OTUD5 (WT) enhanced MyD88 oligomerization (Fig.  xref )."
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"However, transfection of the Flag-OTUD5 mutant C224S did not promote MyD88 oligomerization (Fig.  xref )."
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"HA-YAP and Flag-OTUD5 were constructed by cloning cDNA into pcDNA3-HA or pcDNA3-Flag vector, respectively."
33587979
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"HCT116 cells were co-transfected with plasmids encoding HA-Ub and Myc-PDCD5 or Flag-OTUD5."
25499082
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"To test this hypothesis, we transfected Flag-OTUD5 (WT) or C224S (mutation of cysteine to serine at position 224) [ xref , xref ], Myc-MyD88 and HA-Ubiquitin into HEK293T cells."
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