IndraLab

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JOSD2 binds GST. 3 / 3
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"Subsequently, we examined the biological activity of GST-JOSD2/JOSD1 by introducing their co-reported substrate proteins YAP xref , xref , as shown in Supplementary Fig.  xref , both GST-JOSD2 and GST-JOSD1 efficiently removing the polyubiquitin chains of YAP, confirming that our purified recombinant protein are biologically active."
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"As shown in Supplementary Fig.  xref , GST-JOSD2 efficiently removes K48-linked ubiquitin chains but failed to cleave the K6-, K33-, K63- and K48R-linked ubiquitin chains of KRAS."
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"Given that JOSD2 has been shown to reverse the polyubiquitin chains of KRAS mediated by LZTR1, which is reported to promote K48, K63, and K33-linked ubiquitin chains as part of its function as a “RAS killer protein” and JOSD2 also can deubiquitinate K6-linked ubiquitination of LKB1 and affecting its kinase activity xref , xref , so we next tested the effects of GST-JOSD2 on K6-, K33-, K48-, K63- and K48R-linked ubiquitin chains of KRAS."
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