"We investigated whether this compound improved NGF induced tyrosine phosphorylation of the Y490 domain of TrkA and conserved the expression of an early gene (c-fos) in human NB cell lines (IMR-32 and NB-39)."
"Nogo-A over-expression also changed NGF-induced phosphorylation of TrkA at tyrosines 490 and 674/675 from sustained to transient, and prevented the regulated intramembrane proteolysis of p75 NTR , indicating that Nogo-A was altering the function of the two neurotrophin receptors."
"NGF (50 ng/ml)-induced phosphorylation of TrkA at tyrosine 490, which is known to be the important site for the induction of MAPK and Akt signaling cascades [ xref ], was significantly enhanced by the addition of LPC (1 μM) ( xref C)."
"The Tyr-490 phosphorylation of TrkA stimulated by Ac-NGF(1–14) (50 μM) was inhibited by pre-treatment with BCS, but this effect was not restored by the presence of CuSO 4 and ZnSO 4 (Figure xref )."
"The presence of BCS totally blocked the Tyr 490 phosphorylation of TrkA stimulated by NGF (1-14) (50 muM) (P < 0.01); this effect was partially inhibited by the presence of metal ions (p < 0.05)."
"Moreover, the upregulation of TrkA tyrosine-490 phosphorylation by NGF was not altered by either wortmannin or SP600125 4 h after NGF treatment, indicating a drug specificity of SP600125 and wortmannin."
"However, the upregulation of TrkA tyrosine-490 phosphorylatin by NGF was significantly downregulated during the long-term inactivation of JNK caused by SP600125 treatment for 12 h, but not by wortmannin, suggesting a role of JNK in the maintenance of TrkA tyrosine-490 phosphorylation enhanced by NGF ( xref )."
"At the molecular level, NS 1231 enhanced NGF induced signalling events, such as TrkA phosphorylation at the Shc binding site Tyr490 as well as ERK activation in PC12 cells."
"SgG2 significantly increased NGF-dependent TrkA phosphorylation at tyrosine 490 (Tyr490) at 15 min, but not at 120 min post-incubation, when compared to control and M3 conditions ( xref )."
"NGF-induced phosphorylation of trkA at Y490 provides a recruitment site for binding of the adaptor protein Shc."