IndraLab

Statements

MYC binds OTUD4. 4 / 4
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"We transfected Huh7 cells with plasmids encoding SHBs-Flag and OTUD4-myc or TRIM21-myc."
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"To investigate the interaction between SPARC and OTUD4 proteins, Caco‐2 cells co‐transfected with the SPARC‐Flag plasmids and OTUD4‐Myc plasmids (MiaolingBio) were harvested using IP lysis buffer."
39888301
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"To assess whether OTUD4 also impacts the stability of SHBs, we co-transfected with plasmids encoding OTUD4-myc and SHBs-Flag into Huh7 cells and treated these cells with cycloheximide at various intervals."
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"The pLV2‐SPARC‐3×Flag (Flag‐SPARC), pLV2‐CMV‐OTUD4‐3×Myc (Myc‐OTUD4), and pCMV‐MYD88‐6×His (His‐MYD88) plasmids were obtained from Miaolingbio (Wuhan, China)."
39888301