IndraLab

Statements

Tmc1 binds USP20. 13 / 13
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"The angiogenic sprouting of MCECs is diminished by the expression of WT and phospho-resistant USP20-S334A, while it is exacerbated by USP20-DN and phospho-mimetic USP20-S334D."
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"To test the role of USP20 and its phosphorylation status in EC sprouting, we performed the spheroid-based sprouting assay using MCECs transduced with recombinant adenoviruses, expressing USP20-WT, USP20-DN, USP20-S334A, and USP20-S334D ( xref – xref )."
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"NFκB activity was augmented in MCECs with overexpression of USP20-DN but diminished with exogenous expression of USP20 WT ( xref )."
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"We therefore evaluated the effect of USP20 (USP20-WT), inactive USP20 (USP20-dominant negative or USP20-DN), phospho-resistant USP20 (USP20-S334A), and phospho-mimetic USP20 (USP20-S334D) on NFκB activation triggered by IL-1β."
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"Assessed by scratch-wound healing and spheroid assays, migration and angiogenesis of MCECs, respectively, were (a) increased by USP20-DN or USP20(S334D), and (b) decreased by USP20-WT or USP20(S334A)."
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"USP20-WT and USP20-S334A transduced MCECs formed weak or no sprouts from spheroids compared with the GFP-treated MCECs ( xref ), whereas angiogenic sprouting was significantly increased in USP20-DN and USP20-S334D transduced MCECs compared with the GFP-treated MCECs ( xref )."
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"Data from our in vitro spheroid assay also demonstrated that MCECs transduced with USP20-DN adenovirus increased angiogenic sprouting compared to the GFP control, whereas MCECs transduced with USP20-WT adenovirus decreased angiogenic sprouting ( xref )."
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"We detected significantly more NFκB activity in cells expressing USP20-DN and USP20-S334D than in cells expressing USP20, USP20-S334A, and GFP ( xref – xref )."
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"Similarly, NFκB activity is elevated in the MCECs transduced with recombinant AVs encoding USP20-DN and USP20-S334D mutants ( xref )."
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"Vascular smooth muscle cell-specific expression of dominant-negative-USP20 (USP20-DN) increases vascular inflammation, NFκB activation, and atherosclerotic lesions in Ldlr −/− mice xref ."
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"To test the role of USP20 and its phosphorylation status in EC migration, we performed the scratch-wound healing assay using MCECs transduced with recombinant AVs, encoding GFP, USP20-WT, USP20-DN, USP20-S334A, and USP20-S334D."
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"MCEC migration was significantly increased in cells expressing USP20-DN compared with cells expressing control GFP protein ( p < 0.05) ( xref , xref and xref )."
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"Similarly, cytokine-induced NFκB activity was elevated in mouse coronary endothelial cells (MCECs) expressing catalytically inactive USP20 (USP20-DN) or phospho-mimetic USP20(S334D)."
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