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CALM1 binds IQ. 20 / 20
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"Indeed, electrophysiological experiments confirmed these results, showing that scoparone did not promote CDF or block CDI in L-VGCC mutants (in which the combination of two key positions, Ile and Gln, in the IQ motif was replaced with Ala) because CaM1 binding to the IQ region of the Ca channel was impaired.Notably, it has been reported that CaM binds to the N-termini of Ca channels [36], which was confirmed in this study."
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"These results demonstrated that scoparone blocked CDI or promoted CDF of L-VGCC mediated by CaM by strengthening the CaM1-IQ interaction."
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"The fission yeast Class I myosin, Myo1 contains an IQ motif neck region which binds Cam1 [ xref , xref ]."
35100446
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"There is little evidence supporting this scenario, but the discovery that scoparone facilitates CaM1 binding to the IQ motif is consistent with this hypothesis."
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"Although CaM1 bound to a well-characterized IQ motif, mutations of the IQ motif that markedly weakened CaM1 binding blocked CDF and heightened CDI of Ca 2+ channels during a single depolarizing pulse, suggesting that CaM interactions with the IQ motif actively suppressed inactivation or promoted facilitation of Ca 2+ channels."
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"Indeed, electrophysiological experiments confirmed these results, showing that scoparone did not promote CDF or block CDI in L-VGCC mutants (in which the combination of two key positions, Ile and Gln, in the IQ motif was replaced with Ala) because CaM1 binding to the IQ region of the Ca 2+ channel was impaired."
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"The interaction of Cam1 with the IQ motifs of Myo1 is tightly controlled by cellular calcium concentrations, when local or whole cell calcium concentrations rise, Cam1 dissociates from Myo1 [ xref , xref ]."
35100446
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"Both unmodified and acetylated Cam1 bound to the two Myo1 IQ motifs (comparable amplitudes and 2 rates consistent with cooperative binding to the 2 IQ motifs – Fig.  xref )."
35100446
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"Thus, we hypothesized that yeast Cam1 may interact with the HsMyo1e IQ domain."
31542284
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"This recruitment was specific for the IQ motif-Cam1 interaction as cells overexpressing mGFP-HsMyo1e tail produced no such effect ( xref )."
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"Cam1 binding to the IQ motif(s) stabilizes the α-helix and results in a drop in FRET signal in the absence of calcium (Figure 2E–G)."
31566560
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"In conventional myosin-II the ELC and RLC bind to the IQ motifs, while in most other myosin classes CAM binds to the IQ motifs [76] ."
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"Importantly, mutation of the IQ motif significantly weakened CaM1 binding and eliminated the CaM1-mediated enhancement of scoparone-induced L-VGCC activation, indicating that the effect of scoparone was dependent on the CaM1–IQ interaction."
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"To characterize Cam1 binding to the IQ neck region of Myo1, recombinant FRET constructs were produced in which CyPet and YPet were separated by one of the two Myo1 IQ motifs or by both of these motifs (Myo1 IQ1 -FRET, Myo1 IQ2 -FRET or Myo1 IQ12 -FRET) ( xref and xref )."
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"Cam1 binding to the IQ motif(s) stabilizes the α-helix and results in a drop in FRET signal in the absence of calcium ( xref )."
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"Pull-down assays demonstrated that CaM1 was markedly bound to the NT, CT1, CT1B, PreIQ, and IQ peptides, whereas its binding to CT2, CT3, and CT1A was negligible (Fig. 5a-d)."
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"Scoparone facilitated CaM1 binding to the IQ domain of L-VGCC."
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"Although CaM1 binding to the NT region and PreIQ tended to be decreased, while binding to CT1 and CT1B tended to be only slightly increased, CaM1 binding to the IQ domain was markedly increased in the presence of scoparone (47.06%, xref a-b)."
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"Scoparone markedly left-shifted the binding of CaM1 to the IQ peptide under 1 mM Ca 2+ ( xref c-d), changing theK df value of CaM from 0.43 to 0.23 μM (Table S13)."
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"Overall, these results demonstrated that scoparone facilitated CaM1 binding to the IQ domain of L-VGCC."
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