IndraLab
Statements
reach
"These include : (a) overexpression of Nedd4-1 in neurons, which enhances GluA1 ubiquitination, causes a reduction in the number of AMPARs on the plasma membrane; (b) shRNA mediated knockdown of Nedd4-1 increases the rate of GluA1 internalization; (c) overexpression of USP46, which downregulates GluA1 ubiquitination, decreases the accumulation of internalized GluA1 in neurons; and (d) overexpression of GluA1-4KR ubiquitin deficient mutants (where all four C-terminal lysine residues have been substituted with arginines) blocks AMPA induced GluA1 internalization."
reach
"By deubiquitinating K63 ubiquitinated glutaminergic AMPARs (α-amino-3-hydroxy-5methyl-4-isoxazolepropionic acid) receptors GluA1 and GluA2, which are considered to mediate most of the excitatory synaptic transmission in the brain, USP46 upregulates the intracellular trafficking, cell surface density, and signal intensity of AMPARs [116,117]."