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USP22 binds GST. 4 / 4
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"The in vitro deubiquitination assay using a GST-USP22 fusion protein shows that incubation of ubiquitinated Sirt1 with GST-USP22 but not GST or GST-USP22C185A mutant control inhibited Sirt1 ubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Meanwhile, in vitro deubiquitination assays showed that recombinant GST-USP22 WT, but not the catalytically inactive C185A mutant, efficiently removed ubiquitin from the phosphorylation-mimicking mutant VISTA S248D, demonstrating a direct, activity-dependent deubiquitination of VISTA (Figure S5M)."
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"Further, we constructed the truncated GST-USP22 plasmids with the same structure as Fig. xref ."
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"Additionally, EZH2 protein was detected from GST-USP22 pulldown but not GST protein alone ( xref )."
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